##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549062_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1699436
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3605878656213	31.0	31.0	33.0	30.0	34.0
2	31.707060460058514	31.0	31.0	34.0	30.0	34.0
3	31.823989252905083	31.0	31.0	34.0	30.0	34.0
4	35.523241828465444	37.0	35.0	37.0	33.0	37.0
5	35.405861120983666	37.0	35.0	37.0	33.0	37.0
6	35.47906540758228	37.0	35.0	37.0	33.0	37.0
7	35.86875292744181	37.0	35.0	37.0	35.0	37.0
8	35.88410566799809	37.0	35.0	37.0	35.0	37.0
9	37.57922098861034	39.0	37.0	39.0	35.0	39.0
10	36.96476948823021	39.0	37.0	39.0	32.0	39.0
11	36.6273969717012	39.0	35.0	39.0	32.0	39.0
12	35.914231545053774	37.0	35.0	39.0	31.0	39.0
13	35.577948801837785	37.0	35.0	39.0	30.0	39.0
14	36.607011384953594	38.0	35.0	41.0	31.0	41.0
15	36.80428918770698	38.0	35.0	41.0	31.0	41.0
16	36.9434118142725	38.0	35.0	41.0	32.0	41.0
17	36.8731149628465	38.0	35.0	41.0	32.0	41.0
18	36.81851802598038	38.0	35.0	40.0	32.0	41.0
19	36.785007496604756	38.0	35.0	40.0	31.0	41.0
20	36.64226249179139	38.0	35.0	40.0	31.0	41.0
21	36.44261684464728	38.0	35.0	40.0	31.0	41.0
22	36.388973753645324	38.0	35.0	40.0	31.0	41.0
23	36.34720107141428	38.0	35.0	40.0	31.0	41.0
24	36.26996368206864	38.0	35.0	40.0	31.0	41.0
25	36.15829486959203	38.0	35.0	40.0	30.0	41.0
26	36.05226969418089	38.0	35.0	40.0	30.0	41.0
27	36.01778766602567	38.0	35.0	40.0	30.0	41.0
28	35.99827942917533	37.0	35.0	40.0	30.0	41.0
29	35.98720104787706	37.0	35.0	40.0	30.0	41.0
30	35.90526151028929	37.0	35.0	40.0	30.0	41.0
31	35.75914479862731	37.0	34.0	40.0	30.0	41.0
32	35.60098703334518	37.0	34.0	40.0	29.0	41.0
33	35.45007696671131	37.0	34.0	40.0	29.0	41.0
34	35.27116113816584	37.0	34.0	40.0	27.0	41.0
35	35.15118015624007	37.0	34.0	40.0	27.0	41.0
36	34.97891476937055	37.0	34.0	40.0	26.0	41.0
37	34.91268985710553	37.0	34.0	40.0	26.0	41.0
38	34.90849846655008	37.0	34.0	40.0	27.0	41.0
39	34.85261816273164	36.0	34.0	40.0	26.0	41.0
40	34.66231149628465	36.0	34.0	40.0	25.0	41.0
41	34.63599276465839	36.0	34.0	40.0	25.0	41.0
42	34.5490968768462	36.0	33.0	40.0	25.0	41.0
43	34.488959866685185	36.0	33.0	40.0	25.0	41.0
44	34.41215673905931	36.0	33.0	40.0	24.0	41.0
45	34.37223937824078	35.0	33.0	40.0	24.0	41.0
46	34.337464900119805	35.0	33.0	40.0	24.0	41.0
47	34.27401326087008	35.0	33.0	40.0	24.0	41.0
48	34.23127437573407	35.0	33.0	40.0	24.0	41.0
49	34.145171692255545	35.0	33.0	40.0	24.0	41.0
50	34.0331262842496	35.0	33.0	39.0	24.0	41.0
51	33.89329224519194	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	3.0
13	7.0
14	23.0
15	59.0
16	172.0
17	378.0
18	885.0
19	1750.0
20	3015.0
21	4590.0
22	7015.0
23	9763.0
24	14333.0
25	19713.0
26	26198.0
27	31075.0
28	34891.0
29	39627.0
30	46781.0
31	57595.0
32	71294.0
33	92841.0
34	156517.0
35	233811.0
36	123271.0
37	152203.0
38	213542.0
39	357881.0
40	200.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.994256918177555	23.474081989554183	27.132766400146874	14.398894692121386
2	30.815929520146685	25.189062724339134	30.108047611089795	13.886960144424384
3	29.93875615204103	25.01765291543783	30.897780204726743	14.145810727794398
4	26.214344052968165	28.156988553849633	30.419445039413073	15.209222353769134
5	22.995334922880296	32.83324585333016	29.075646273234177	15.09577295055536
6	20.619782092411835	42.80631927298233	26.354449358493053	10.219449276112782
7	86.33081798902694	4.855434391174484	6.8425053959078195	1.9712422238907494
8	87.23211700823097	3.8661061669871657	6.8585107059047825	2.043266118877086
9	83.17836035013968	5.501413410096055	8.300871583278218	3.0193546564860343
10	47.99986583784267	24.67748123495089	14.97090799535846	12.351744931847977
11	40.71803821973879	22.811862288429808	21.07711028835449	15.392989203476917
12	38.63858362421415	20.797370421716384	25.998625426317908	14.56542052775156
13	22.46557093059109	36.13822468160025	24.497362654433587	16.898841733375072
14	16.741554256824028	38.57026684146976	27.208320878220775	17.47985802348544
15	16.58179537211169	24.083931374879665	43.38333423559346	15.95093901741519
16	17.220889754012507	19.30234501328676	42.445787896690426	21.030977336010302
17	16.892310154663075	19.665583169945794	30.130054912335623	33.31205176305551
18	20.465613297588142	24.257047632273295	34.00128042479976	21.276058645338807
19	28.209594241854358	24.303592485977703	26.13837767353404	21.3484355986339
20	30.591619807983356	22.243850312691976	26.54033455805338	20.624195321271294
21	22.726363334659265	26.491965569753727	28.439082142546113	22.34258895304089
22	23.474552733965854	23.81531284496739	25.531823499090283	27.17831092197647
23	20.56694103220127	28.899882078524875	25.330403733944674	25.20277315532918
24	19.567197587905635	23.51950882528086	37.97718772581021	18.9361058610033
25	19.857999948218115	25.27126646722795	33.61697645571825	21.253757128835684
26	18.773581352872366	31.677038735203915	27.237212816487354	22.31216709543637
27	18.179737277543843	32.96958520356165	27.91973337036523	20.930944148529278
28	16.44775090088712	28.66221499368026	35.692135508486345	19.19789859694628
29	18.19427151125432	24.4718247701002	34.40541450222309	22.928489216422392
30	19.202429511908655	29.13384205112755	31.026764173525805	20.63696426343799
31	26.67743886795384	28.59130911667165	24.499892905646345	20.23135910972817
32	28.24790106835444	25.796381858451863	26.63230624748446	19.323410825709235
33	27.17219124462469	27.686361828277146	24.186671342727823	20.954775584370346
34	20.14927305294227	27.426157854723566	27.80210611049784	24.62246298183633
35	21.498308850701058	25.23543104888916	30.166184545931706	23.100075554478074
36	28.74388914910594	27.043148432774167	25.28491805516654	18.928044362953354
37	21.069637220819143	32.49013202027026	28.53646739271146	17.903763366199136
38	21.127068039043543	31.749709903756308	25.612909224001374	21.51031283319878
39	21.698139853457267	30.99357669250269	26.843552802223797	20.46473065181625
40	24.08510823590885	25.999213856832505	26.97088916558199	22.94478874167665
41	18.118010916562906	25.935251459896108	28.234426009570235	27.71231161397075
42	21.954460185614522	26.845494622921954	24.30376901513208	26.89627617633144
43	21.410338488769217	26.000508403964613	27.224267345166282	25.3648857620999
44	20.17486978032712	28.658154823129557	28.12180040907689	23.045174987466428
45	18.777641523423064	35.15148555167715	23.71457354086885	22.356299384030937
46	21.571744978922418	32.42258019719483	25.90224050802737	20.103434315855377
47	21.416340480018075	28.893821244224554	27.166130410324367	22.523707865433
48	22.540419292047478	25.5000482513022	29.99895259368402	21.960579862966302
49	21.236751486963907	25.766548431361937	31.134329271593636	21.86237081008052
50	19.958915781471028	32.34826142320158	27.112995134856504	20.579827660470887
51	19.078976789946783	31.807258408083623	26.282543149609634	22.83122165235996
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1213.0
1	2461.0
2	3709.0
3	7931.5
4	12154.0
5	9282.5
6	6411.0
7	6591.5
8	6772.0
9	6917.5
10	7063.0
11	7333.0
12	7603.0
13	7246.5
14	6890.0
15	6894.5
16	6899.0
17	6323.5
18	5748.0
19	6502.5
20	7257.0
21	7283.0
22	7309.0
23	7506.5
24	7704.0
25	8728.0
26	11310.0
27	12868.0
28	17122.5
29	21377.0
30	24811.0
31	28245.0
32	35403.0
33	42561.0
34	51010.0
35	59459.0
36	60064.5
37	60670.0
38	66736.0
39	72802.0
40	90415.5
41	108029.0
42	126140.5
43	144252.0
44	148793.5
45	153335.0
46	150675.5
47	148016.0
48	136089.0
49	124162.0
50	115517.0
51	106872.0
52	100280.5
53	93689.0
54	86231.0
55	78773.0
56	72280.0
57	65787.0
58	64886.0
59	63985.0
60	60904.5
61	57824.0
62	53045.5
63	48267.0
64	40802.0
65	33337.0
66	28506.0
67	23675.0
68	20801.5
69	17928.0
70	16247.0
71	14566.0
72	11807.0
73	9048.0
74	7481.5
75	4734.5
76	3554.0
77	2824.0
78	2094.0
79	1665.0
80	1236.0
81	771.0
82	306.0
83	243.0
84	180.0
85	125.0
86	70.0
87	48.0
88	26.0
89	23.0
90	20.0
91	13.5
92	7.0
93	8.0
94	9.0
95	7.0
96	5.0
97	4.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1699436.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.664114668648452
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.11248390305236	12.548911342476654
2	6.558545904794732	2.0546763022459875
3	2.290646294124022	1.0764283864941997
4	1.178403458568755	0.7383458760381162
5	0.7303123871117773	0.5719848487826628
6	0.47094529417164627	0.4426164654339027
7	0.36264514692966626	0.39763606158746106
8	0.29100856137982445	0.3646713180009593
9	0.2603249217814558	0.3669983483302496
>10	4.01244367567646	16.095329123935308
>50	2.0640076746404517	23.49898844373599
>100	1.6606653802738083	40.433243033930644
>500	0.0056755481212365285	0.5395785217403289
>1k	0.0015134794989964076	0.5070402299526048
>5k	3.783698747491019E-4	0.36355169731493603
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6134	0.36094327765211515	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4206	0.24749387443834306	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1809	0.10644708009010048	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.014122332350262086	0.0
2	0.0	0.0	0.0	0.05007543679197098	0.0
3	0.0	0.0	0.0	0.06943480072212192	0.0
4	0.0	0.0	0.0	0.1141555198312852	0.0
5	0.0	0.0	0.0	0.19294636573545576	0.0
6	0.0	0.0	0.0	0.28409425244610564	0.0
7	0.0	0.0	0.0	0.33834754589169586	0.0
8	0.0	0.0	0.0	0.4725097032191857	0.0
9	5.884305145942536E-5	0.0	0.0	0.5196429874381854	0.0
10	5.884305145942536E-5	0.0	0.0	0.6018467303270026	0.0
11	5.884305145942536E-5	0.0	0.0	0.7025271913740794	0.0
12	5.884305145942536E-5	0.0	0.0	0.7961464862460251	0.0
13	5.884305145942536E-5	0.0	0.0	0.8316288462760586	0.0
14	5.884305145942536E-5	0.0	0.0	0.8438093579281597	0.0
15	5.884305145942536E-5	0.0	0.0	0.8652282286593905	0.0
16	5.884305145942536E-5	0.0	0.0	0.9144210196794701	0.0
17	5.884305145942536E-5	0.0	0.0	0.9740878738593275	0.0
18	5.884305145942536E-5	0.0	0.0	1.057998065240468	0.0
19	1.1768610291885073E-4	0.0	0.0	1.096010676483257	0.0
20	1.765291543782761E-4	0.0	0.0	1.1424966871362028	0.0
21	1.765291543782761E-4	0.0	0.0	1.1989271734857918	0.0
22	1.765291543782761E-4	0.0	0.0	1.2611242788784043	0.0
23	1.765291543782761E-4	0.0	0.0	1.3379144610329545	0.0
24	1.765291543782761E-4	0.0	0.0	1.3933446155077331	0.0
25	1.765291543782761E-4	0.0	0.0	1.4437142675570012	0.0
26	1.765291543782761E-4	0.0	0.0	1.491553668393514	0.0
27	1.765291543782761E-4	0.0	0.0	1.5375100915833253	0.0
28	1.765291543782761E-4	0.0	0.0	1.5857025507285947	0.0
29	1.765291543782761E-4	0.0	0.0	1.6429568397986156	0.0
30	1.765291543782761E-4	0.0	0.0	1.7180405734608424	0.0
31	1.765291543782761E-4	0.0	0.0	1.7875342172344237	0.0
32	1.765291543782761E-4	0.0	0.0	1.8527911613029264	0.0
33	2.3537220583770145E-4	0.0	0.0	1.9205195135327249	0.0
34	2.3537220583770145E-4	0.0	0.0	1.9905427447694413	0.0
35	2.942152572971268E-4	0.0	0.0	2.0811022009654967	0.0
36	2.942152572971268E-4	0.0	0.0	2.154185270878103	0.0
37	2.942152572971268E-4	0.0	0.0	2.231093139135572	0.0
38	2.942152572971268E-4	0.0	0.0	2.3045881104083943	0.0
39	2.942152572971268E-4	0.0	0.0	2.386144579731158	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	20	7.03424E-4	45.000004	1
ACGTTAT	30	2.166118E-6	45.000004	11
TAGCGAA	20	7.03424E-4	45.000004	33
CGCGATA	20	7.03424E-4	45.000004	37
TCTAGCG	20	7.03424E-4	45.000004	1
CGCAATA	20	7.03424E-4	45.000004	13
ACGTTCA	25	3.8915976E-5	45.0	21
CCGTATG	25	3.8915976E-5	45.0	38
CTACGTT	35	1.2123928E-7	45.0	23
TATAGCG	130	0.0	43.269234	1
TACGGGA	345	0.0	42.391304	4
TACGGGC	145	0.0	41.896553	4
AGGGTAC	925	0.0	41.837837	6
GTATGCG	70	0.0	41.785713	1
AACGGGA	305	0.0	41.311478	4
TATTTCG	60	3.6379788E-12	41.250004	17
ATACCGG	60	3.6379788E-12	41.250004	2
TATGCCA	775	0.0	41.225807	10
ACGGGTA	230	0.0	41.086956	5
CAGGGTA	570	0.0	40.657894	5
>>END_MODULE