##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549059_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	992085
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32461432236149	31.0	31.0	33.0	30.0	34.0
2	31.668749149518437	31.0	31.0	34.0	30.0	34.0
3	31.768596440829164	31.0	31.0	34.0	30.0	34.0
4	35.49534666888422	37.0	35.0	37.0	33.0	37.0
5	35.35172591058226	37.0	35.0	37.0	33.0	37.0
6	35.43032603053166	37.0	35.0	37.0	33.0	37.0
7	35.844680647323564	37.0	35.0	37.0	35.0	37.0
8	35.871196520459435	37.0	35.0	37.0	35.0	37.0
9	37.58140381116537	39.0	37.0	39.0	35.0	39.0
10	36.970572077997346	39.0	37.0	39.0	32.0	39.0
11	36.54274482529219	39.0	35.0	39.0	32.0	39.0
12	35.58385722997525	37.0	35.0	39.0	31.0	39.0
13	35.143734659832575	37.0	35.0	39.0	30.0	39.0
14	36.0810454749341	38.0	35.0	40.0	30.0	41.0
15	36.37020114203924	38.0	35.0	40.0	31.0	41.0
16	36.585128290418666	38.0	35.0	40.0	32.0	41.0
17	36.55419948895508	38.0	35.0	40.0	32.0	41.0
18	36.51044819748308	38.0	35.0	40.0	31.0	41.0
19	36.413906066516475	37.0	35.0	40.0	31.0	41.0
20	36.22168564185529	37.0	35.0	40.0	31.0	41.0
21	36.009684654036704	36.0	34.0	40.0	31.0	41.0
22	35.94002429227334	36.0	34.0	40.0	30.0	41.0
23	35.9247645111054	36.0	34.0	40.0	30.0	41.0
24	35.84658068613072	36.0	34.0	40.0	30.0	41.0
25	35.74175196681736	36.0	34.0	40.0	30.0	41.0
26	35.637503842916686	36.0	34.0	40.0	30.0	41.0
27	35.576463710266765	36.0	34.0	40.0	30.0	41.0
28	35.578697389840585	36.0	34.0	40.0	30.0	41.0
29	35.56367952342793	36.0	34.0	40.0	30.0	41.0
30	35.50243678717045	36.0	34.0	40.0	30.0	41.0
31	35.27160172767454	36.0	34.0	40.0	29.0	41.0
32	35.05573917557467	35.0	34.0	40.0	29.0	41.0
33	34.86544499715246	35.0	34.0	40.0	27.0	41.0
34	34.685928121078334	35.0	34.0	40.0	26.0	41.0
35	34.526357116577714	35.0	33.0	40.0	25.0	41.0
36	34.297557164960665	35.0	33.0	40.0	24.0	41.0
37	34.23772156619644	35.0	33.0	40.0	24.0	41.0
38	34.259968651879625	35.0	33.0	40.0	24.0	41.0
39	34.21490497286019	35.0	33.0	40.0	24.0	41.0
40	34.03771753428386	35.0	33.0	40.0	23.0	41.0
41	34.017501524566946	35.0	33.0	40.0	23.0	41.0
42	33.91516452723305	35.0	33.0	40.0	23.0	41.0
43	33.813267008371255	35.0	33.0	40.0	23.0	41.0
44	33.753625949389416	35.0	33.0	40.0	23.0	41.0
45	33.726341996905504	35.0	33.0	40.0	23.0	41.0
46	33.694543310301036	35.0	33.0	39.0	23.0	41.0
47	33.63477020618193	35.0	33.0	39.0	23.0	41.0
48	33.62093772207019	35.0	33.0	39.0	23.0	41.0
49	33.580693186571715	35.0	33.0	39.0	24.0	41.0
50	33.42014948315921	35.0	33.0	39.0	23.0	41.0
51	33.247000005039894	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	2.0
12	0.0
13	2.0
14	11.0
15	41.0
16	106.0
17	284.0
18	627.0
19	1185.0
20	2099.0
21	3262.0
22	4950.0
23	6998.0
24	9943.0
25	13975.0
26	18139.0
27	21015.0
28	22887.0
29	25481.0
30	30068.0
31	35804.0
32	44405.0
33	57459.0
34	100094.0
35	162248.0
36	64780.0
37	77850.0
38	107725.0
39	180503.0
40	141.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.81807002424188	23.52369000640066	27.539374146368505	15.118865822988958
2	31.353664252559003	25.791842432856054	29.517732855551692	13.336760459033247
3	28.49221588875953	24.268686654873324	32.981649757833246	14.257447698533895
4	25.989002958415863	27.484842528613978	32.06670799377069	14.459446519199465
5	22.76286810101957	32.085657982934926	30.795042763472885	14.356431152572613
6	21.246566574436663	41.95134489484268	26.653562950755227	10.148525579965426
7	86.06026701341115	4.675506635016153	7.474057162440719	1.7901691891319798
8	87.31066390480655	3.541128028344345	7.350579839429081	1.7976282274200295
9	83.64696573378289	5.1795965063477425	8.492215888759532	2.6812218711098343
10	54.11401240821099	21.540795395555826	14.157456266348145	10.18773592988504
11	46.37939289476204	20.471128985923585	20.631397511301955	12.518080608012419
12	43.53941446549439	20.417504548501388	22.880095959519597	13.162985026484625
13	21.20110676000544	42.19608198894248	23.173417600306426	13.429393650745652
14	14.501479207930773	43.17472797189757	27.01945901812849	15.304333802043171
15	13.585630263535887	23.01899534818085	48.79672608697844	14.598648301304829
16	14.528089831012464	17.861271967623743	48.31995242343146	19.290685777932335
17	14.74198279381303	18.567461457435602	30.78738212955543	35.90317361919594
18	20.70497991603542	22.26946279804654	36.80773320834405	20.217824077573997
19	28.871316469858932	24.527737038661	25.478865218202067	21.122081273277995
20	31.46444105091802	21.34938034543411	27.765866836006996	19.42031176764088
21	20.58825604660891	28.27076308985621	29.348090133405908	21.79289073012897
22	22.048715583846143	24.344385813715558	25.614438278978113	27.99246032346019
23	18.70525207013512	30.302443843017485	25.899494498959264	25.092809587888134
24	18.767242726177695	23.023732845471912	41.36833033459835	16.84069409375205
25	16.14750752203692	25.89082588689477	37.12040802955392	20.841258561514387
26	16.053261565289265	35.04296506851731	27.292318702530526	21.61145466366289
27	16.119284133919976	36.09851978409108	28.749855103141364	19.03234097884758
28	14.606107339592878	28.995600175388198	38.82883019096146	17.569462294057463
29	14.950936663693131	24.023949560773524	38.06649631835982	22.958617457173528
30	17.80129726787523	33.29432457904313	30.94986820685727	17.954509946224366
31	29.01979165091701	28.22137216065156	24.397405464249537	18.361430724181897
32	29.910945130709564	26.138889308879783	26.454487266716058	17.495678293694592
33	28.01503903395374	29.32641860324468	23.480447743892913	19.178094618908663
34	18.18684890911565	28.16421979971474	27.908193350368165	25.740737940801445
35	20.592791948270563	25.488743404043003	31.20639864527737	22.712066002409067
36	30.361309766804258	25.359722201222677	25.81643709964368	18.462530932329386
37	19.250265854236282	33.92370613405101	29.165948482236903	17.6600795294758
38	19.189484771970143	33.58119515968894	25.411633075794914	21.817686992546
39	19.931356688186998	32.72330495874849	27.337879314776455	20.00745903828805
40	24.425124863292965	26.817560995277624	25.6021409455843	23.155173195845112
41	17.118795264518667	24.501932798096938	29.109602503817715	29.26966943356668
42	21.094865863308083	25.265879435733833	24.579143924159723	29.06011077679836
43	20.94467711940005	26.243416642727187	26.908984613213587	25.902921624659175
44	18.554055348080052	30.36584566846591	28.678288654701966	22.401810328752074
45	16.84462520852548	39.24835069575692	22.20706895074515	21.699955144972456
46	21.045273338474022	35.2546404793944	24.77368370653724	18.92640247559433
47	19.77290252347329	28.87686034966762	27.881885120730583	23.468352006128505
48	21.98148344143899	25.21527893275274	32.32182726278494	20.48141036302333
49	20.601258964705647	25.237857643246294	32.15712363355963	22.003759758488435
50	19.208031569875565	34.457934551978916	27.57586295529113	18.758170922854394
51	17.725900502477106	34.78260431313849	24.29872440365493	23.192770780729475
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1363.0
1	1895.0
2	2427.0
3	6379.0
4	10331.0
5	7473.0
6	4615.0
7	4858.5
8	5102.0
9	5431.0
10	5760.0
11	5903.5
12	6047.0
13	6078.5
14	6110.0
15	5905.0
16	5700.0
17	5221.0
18	4742.0
19	4362.0
20	3982.0
21	4084.5
22	4187.0
23	4531.5
24	4876.0
25	4840.5
26	6038.0
27	7271.0
28	9520.5
29	11770.0
30	14723.5
31	17677.0
32	18699.5
33	19722.0
34	23540.5
35	27359.0
36	28382.5
37	29406.0
38	34385.5
39	39365.0
40	54939.0
41	70513.0
42	87205.0
43	103897.0
44	105527.5
45	107158.0
46	99745.0
47	92332.0
48	84967.0
49	77602.0
50	70681.0
51	63760.0
52	57081.5
53	50403.0
54	46842.5
55	43282.0
56	39200.0
57	35118.0
58	32522.5
59	29927.0
60	27993.5
61	26060.0
62	23550.5
63	21041.0
64	18273.0
65	15505.0
66	12595.0
67	9685.0
68	8449.0
69	7213.0
70	6333.5
71	5454.0
72	5016.0
73	4578.0
74	3517.5
75	2135.0
76	1813.0
77	1391.0
78	969.0
79	699.0
80	429.0
81	297.5
82	166.0
83	123.0
84	80.0
85	49.5
86	19.0
87	11.5
88	4.0
89	3.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	992085.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.115064823272352
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.13055136290885	14.934705539800127
2	7.275840106157069	2.7815631054591434
3	2.785083477767969	1.597111536472786
4	1.5966206432032684	1.2207802837202109
5	0.9995086188267209	0.9552836020146092
6	0.7797182724185568	0.8942619192702386
7	0.6094225232686392	0.8154405725929568
8	0.4835200960192841	0.7394014383010789
9	0.3938816484289716	0.6776165918175447
>10	4.211964256986358	19.16170091410114
>50	1.5561565774509973	21.152351917380702
>100	1.1729556739995448	33.552146424877535
>500	0.0026537458687772505	0.3663469585391478
>1k	0.0015922475212663502	0.5210628576729612
>5k	5.307491737554501E-4	0.6302263379798355
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6212	0.6261560249373793	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2562	0.2582440012700525	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1555	0.1567406018637516	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1019	0.10271297318274139	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02691301652580172	0.0
2	0.0	0.0	0.0	0.0814446342803288	0.0
3	0.0	0.0	0.0	0.11662307161180746	0.0
4	0.0	0.0	0.0	0.16833235055463996	0.0
5	0.0	0.0	0.0	0.27074293029327123	0.0
6	0.0	0.0	0.0	0.3807133461346558	0.0
7	0.0	0.0	0.0	0.4436111825095632	0.0
8	0.0	0.0	0.0	0.5863408881295453	0.0
9	0.0	0.0	0.0	0.6327078828930989	0.0
10	0.0	0.0	0.0	0.7259458614937229	0.0
11	1.0079781470337723E-4	0.0	0.0	0.8554710533875626	0.0
12	1.0079781470337723E-4	0.0	0.0	0.9737068900346241	0.0
13	1.0079781470337723E-4	0.0	0.0	1.0230978192392788	0.0
14	1.0079781470337723E-4	0.0	0.0	1.04103983025648	0.0
15	1.0079781470337723E-4	0.0	0.0	1.0710775790380864	0.0
16	1.0079781470337723E-4	0.0	0.0	1.141232858071637	0.0
17	1.0079781470337723E-4	0.0	0.0	1.2205607382431949	0.0
18	1.0079781470337723E-4	0.0	0.0	1.3225681267230127	0.0
19	1.0079781470337723E-4	0.0	0.0	1.3773013401069465	0.0
20	1.0079781470337723E-4	0.0	0.0	1.4297162037527027	0.0
21	1.0079781470337723E-4	0.0	0.0	1.5045081822626085	0.0
22	1.0079781470337723E-4	0.0	0.0	1.585549625284124	0.0
23	2.0159562940675447E-4	0.0	0.0	1.6898753635021193	0.0
24	2.0159562940675447E-4	0.0	0.0	1.7625505879032544	0.0
25	2.0159562940675447E-4	0.0	0.0	1.823432467984094	0.0
26	2.0159562940675447E-4	0.0	0.0	1.8824999874002732	0.0
27	2.0159562940675447E-4	0.0	0.0	1.9386443701900542	0.0
28	2.0159562940675447E-4	0.0	0.0	2.0015422065649617	0.0
29	2.0159562940675447E-4	0.0	0.0	2.0745198244102068	0.0
30	2.0159562940675447E-4	0.0	0.0	2.1565692455787557	0.0
31	2.0159562940675447E-4	0.0	0.0	2.2440617487412875	0.0
32	2.0159562940675447E-4	0.0	0.0	2.3207688857305575	0.0
33	3.023934441101317E-4	0.0	0.0	2.3966696402022003	0.0
34	3.023934441101317E-4	0.0	0.0	2.4839605477353253	0.0
35	3.023934441101317E-4	0.0	0.0	2.6030027669000138	0.0
36	3.023934441101317E-4	0.0	0.0	2.6891848984714013	0.0
37	3.023934441101317E-4	0.0	0.0	2.7838340464778724	0.0
38	3.023934441101317E-4	0.0	0.0	2.873544101563878	0.0
39	3.023934441101317E-4	0.0	0.0	2.971015588382044	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATACG	35	1.2117016E-7	45.000004	1
CTTACCG	55	1.8189894E-12	45.000004	1
TTTAGCG	50	2.1827873E-11	45.0	1
CTATGCG	20	7.0328184E-4	45.0	1
GTACCTA	20	7.0328184E-4	45.0	22
CCTTAGT	40	6.8139343E-9	45.0	43
GATACCC	40	6.8139343E-9	45.0	3
TACTTCG	25	3.890419E-5	45.0	29
GATCGAC	25	3.890419E-5	45.0	9
GCGCAAA	25	3.890419E-5	45.0	10
ATCCGTT	25	3.890419E-5	45.0	33
ATATCGT	20	7.0328184E-4	45.0	1
TACGCGG	40	6.8139343E-9	45.0	2
GCCGACC	25	3.890419E-5	45.0	14
TACGCAT	25	3.890419E-5	45.0	38
CGTAGGC	45	3.8380676E-10	45.0	39
TATTACG	20	7.0328184E-4	45.0	1
TCGTAAT	25	3.890419E-5	45.0	33
CGTAATC	25	3.890419E-5	45.0	34
ACGTATA	20	7.0328184E-4	45.0	36
>>END_MODULE