FastQCFastQC Report
Sat 18 Jun 2016
SRR3549057_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549057_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5031159
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393490.782106071384347No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC72620.14434049887908532No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT61360.12195996985982752No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCTA5400.042.531
CGTTTTT283150.042.4095041
TACGTAG4250.041.2941171
ACGGGAT17550.038.717955
CGAATAT8950.038.46368814
GTTGATC32750.038.3358816
GGCGATA22900.038.1222728
CGCTATA950.037.89473743
GCGTTAG4400.037.8409081
CGCATCG3350.037.6119421
GGGCGAT98900.037.583427
TAGTAGG23600.037.3728832
GCTACGA9400.037.34042710
ATAGCGG7900.037.3101232
AACGGGA12850.037.295724
CGTATGG5450.037.155962
GGTACCT34850.037.0588238
ACGGGTA3650.036.98635
TATGGGC17250.036.9130444
GTAGCAT33250.036.879729