##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549057_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	5031159
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.055424803708252	31.0	31.0	33.0	30.0	34.0
2	31.345393178788427	31.0	31.0	34.0	30.0	34.0
3	31.42683604314632	31.0	31.0	34.0	30.0	34.0
4	35.218618215007716	37.0	35.0	37.0	33.0	37.0
5	35.09347945473399	37.0	35.0	37.0	32.0	37.0
6	35.1381872447283	37.0	35.0	37.0	32.0	37.0
7	35.74611257565106	37.0	35.0	37.0	35.0	37.0
8	35.73840898290036	37.0	35.0	37.0	35.0	37.0
9	37.389925064980055	39.0	37.0	39.0	35.0	39.0
10	36.6173020967932	39.0	35.0	39.0	32.0	39.0
11	36.281642659275924	38.0	35.0	39.0	31.0	39.0
12	35.76760722529342	37.0	35.0	39.0	30.0	39.0
13	35.549484522353595	37.0	35.0	39.0	30.0	39.0
14	36.55244785545438	38.0	35.0	40.0	31.0	41.0
15	36.75458000830425	38.0	35.0	41.0	31.0	41.0
16	36.821932083641165	38.0	35.0	41.0	31.0	41.0
17	36.730284016068666	38.0	35.0	40.0	31.0	41.0
18	36.61736510414399	38.0	35.0	40.0	31.0	41.0
19	36.53919345423192	38.0	35.0	40.0	31.0	41.0
20	36.40223475346337	38.0	35.0	40.0	31.0	41.0
21	36.279759991683825	38.0	35.0	40.0	30.0	41.0
22	36.173727763324514	38.0	34.0	40.0	30.0	41.0
23	36.09578508649796	38.0	34.0	40.0	30.0	41.0
24	35.994907137699286	38.0	34.0	40.0	30.0	41.0
25	35.8719758210782	37.0	34.0	40.0	30.0	41.0
26	35.699491707576726	37.0	34.0	40.0	29.0	41.0
27	35.60565130221486	37.0	34.0	40.0	29.0	41.0
28	35.573112676423065	37.0	34.0	40.0	29.0	41.0
29	35.52367814255125	37.0	34.0	40.0	29.0	41.0
30	35.423864362068464	37.0	34.0	40.0	28.0	41.0
31	35.28221588703517	37.0	34.0	40.0	27.0	41.0
32	35.103535984452094	37.0	34.0	40.0	27.0	41.0
33	34.85652152913474	37.0	33.0	40.0	25.0	41.0
34	34.65584053296666	37.0	33.0	40.0	24.0	41.0
35	34.477865636923816	37.0	33.0	40.0	24.0	41.0
36	34.30353980067019	37.0	33.0	40.0	23.0	41.0
37	34.241226524544345	37.0	33.0	40.0	23.0	41.0
38	34.17772723938957	36.0	33.0	40.0	23.0	41.0
39	34.10799181659733	36.0	33.0	40.0	23.0	41.0
40	33.969688495235395	36.0	33.0	40.0	23.0	41.0
41	33.87200364766846	36.0	33.0	40.0	23.0	41.0
42	33.80992510870755	36.0	33.0	40.0	23.0	41.0
43	33.763681290931174	36.0	33.0	40.0	23.0	41.0
44	33.6435862989025	35.0	33.0	40.0	22.0	41.0
45	33.58198458844175	35.0	32.0	40.0	22.0	41.0
46	33.50521201973541	35.0	32.0	40.0	22.0	41.0
47	33.387350707858765	35.0	32.0	39.0	22.0	41.0
48	33.30185549691433	35.0	32.0	39.0	22.0	41.0
49	33.259055815966065	35.0	32.0	39.0	21.0	41.0
50	33.221807937296354	35.0	32.0	39.0	21.0	41.0
51	33.05400564760525	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	1.0
11	6.0
12	8.0
13	25.0
14	82.0
15	214.0
16	644.0
17	1877.0
18	3947.0
19	7503.0
20	12840.0
21	19987.0
22	28714.0
23	40993.0
24	57319.0
25	77936.0
26	99144.0
27	113526.0
28	123866.0
29	139335.0
30	161723.0
31	193117.0
32	232526.0
33	289579.0
34	432555.0
35	566329.0
36	407863.0
37	488804.0
38	640408.0
39	889817.0
40	470.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.64706998924105	24.114980265978474	27.241317557246745	15.996632187533727
2	32.855590530929355	25.320686545585218	28.471193218103423	13.352529705381999
3	28.898033236476923	25.242791969007538	32.48583477485009	13.37334001966545
4	25.41748332740031	27.919332304942063	31.885476090101704	14.777708277555925
5	22.35141048016968	32.45761066187731	30.626621023108196	14.564357834844813
6	21.0858969076509	41.135471965803504	27.99086651803292	9.78776460851267
7	85.80551717804983	4.128969090422306	8.368668133923018	1.6968455976048462
8	86.43449749848891	3.521912147876861	8.24466092206587	1.7989294315683524
9	80.7996924764254	6.03103579115667	10.00167158302888	3.1676001493890373
10	42.43557001478188	30.364216277004964	15.31498010696939	11.885233601243769
11	35.58619395650187	24.158707764950382	25.063310461863757	15.191787816683988
12	33.68058930357796	21.276151280450488	28.35736258782519	16.685896828146358
13	22.742672215288763	30.873522383212297	28.298072074446463	18.085733327052473
14	18.660710186261255	33.19984520465364	29.505487701740297	18.63395690734481
15	17.822215517339046	24.312151534069983	40.16901473398078	17.696618214610194
16	19.73233205311142	21.16122746269796	39.317421691502894	19.789018792687727
17	19.72726363845786	20.81824486167104	31.275417851035915	28.17907364883519
18	21.332062850726842	23.471371109519694	33.46545000863618	21.731116031117285
19	27.423959369998048	24.292732549299277	27.663049408694896	20.620258672007783
20	29.156939782662405	23.476837046891184	27.625483511850845	19.74073965859556
21	24.38656778686581	25.886798648184246	29.16918348237454	20.557450082575407
22	23.93879819739348	24.505844478379636	27.203374013820675	24.35198331040621
23	21.525099882551913	28.00742333923456	27.256463172799744	23.211013605413783
24	21.00750542767581	24.245188832235275	35.494445713204456	19.25286002688446
25	21.279450719009276	25.19349915198466	32.25201191216577	21.275038216840294
26	20.092070236698937	29.397858425861717	28.287577474693208	22.22249386274614
27	20.286597978716237	29.809155305964293	28.834747619783034	21.069499095536436
28	19.70472410035143	27.88178628423391	33.43881598653511	18.97467362887955
29	19.873870016829123	24.732154161695146	33.2329985993287	22.160977222147025
30	21.066060524026373	28.011517823229198	30.511001540599292	20.411420112145134
31	26.00993131006196	26.907597235547513	26.516096986797677	20.566374467592855
32	27.307206947743058	26.507729133585322	27.270277087247692	18.914786831423932
33	26.596376699682917	26.838269273541147	25.678834638301034	20.886519388474902
34	21.640381470750576	27.400306768281425	27.94215010895104	23.01716165201696
35	22.568915035283123	25.542404841508688	29.198719420316472	22.68996070289172
36	26.865102057001177	27.491061204784028	25.350540501701495	20.293296236513296
37	22.369795905873776	31.233499080430573	27.244776004892714	19.15192900880294
38	22.473589087524363	30.452366939705144	25.56854196021235	21.50550201255814
39	22.1448974282069	30.30852334422347	25.92498070524108	21.621598522328554
40	24.66552935417068	26.651354091572138	27.23370499719846	21.44941155705872
41	20.298980811379643	26.035492020824623	28.438814992728318	25.226712175067416
42	22.081432926289946	27.216015236250733	25.902262281911582	24.80028955554774
43	22.392136682621242	27.048876809498566	27.000100772008995	23.5588857358712
44	21.567495680418766	28.425895504395708	28.074545050156434	21.932063765029092
45	20.47828343330036	31.206566916291056	25.585198162093466	22.729951488315116
46	22.056210109837515	30.314088662274436	25.92152225759512	21.70817897029293
47	22.091589631732965	28.08752416689673	27.21251703633298	22.60836916503732
48	22.772347286181972	25.907171687477977	29.146723448811695	22.17375757752836
49	21.60571748974739	26.854925475422263	29.41856935946568	22.12078767536466
50	21.018516806962374	30.00479611159178	27.55156416245243	21.425122918993416
51	19.829148711062402	30.857104694961933	26.288932629638616	23.024813964337042
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4643.0
1	6436.5
2	8230.0
3	36945.5
4	65661.0
5	47278.5
6	28896.0
7	29383.5
8	29871.0
9	31071.0
10	32271.0
11	32385.5
12	32500.0
13	31794.0
14	31088.0
15	30347.5
16	29607.0
17	28282.5
18	26958.0
19	26534.5
20	26111.0
21	26591.0
22	27071.0
23	26823.5
24	26576.0
25	30201.5
26	39891.5
27	45956.0
28	54369.0
29	62782.0
30	70873.5
31	78965.0
32	92011.0
33	105057.0
34	120101.5
35	135146.0
36	145111.0
37	155076.0
38	185239.5
39	215403.0
40	246440.0
41	277477.0
42	333664.5
43	389852.0
44	397263.5
45	404675.0
46	399373.5
47	394072.0
48	364182.5
49	334293.0
50	319256.0
51	304219.0
52	289905.0
53	275591.0
54	262442.5
55	249294.0
56	238845.5
57	228397.0
58	227609.0
59	226821.0
60	213095.0
61	199369.0
62	178023.5
63	156678.0
64	134993.5
65	113309.0
66	97334.5
67	81360.0
68	70992.0
69	60624.0
70	53103.5
71	45583.0
72	40045.5
73	34508.0
74	29182.0
75	18669.5
76	13483.0
77	10435.0
78	7387.0
79	6124.5
80	4862.0
81	3666.5
82	2471.0
83	1630.0
84	789.0
85	561.5
86	334.0
87	224.0
88	114.0
89	64.5
90	15.0
91	19.0
92	23.0
93	14.5
94	6.0
95	4.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	5031159.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.042511772032974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.83813424592738	13.776848545153994
2	6.7615726865339845	2.304683642154241
3	2.3002132915795834	1.1760423629979528
4	1.2684960444274407	0.8647343507972768
5	0.8244133304824975	0.7025036944884439
6	0.6074297444636034	0.6211277142422366
7	0.481432584090632	0.5743374337263395
8	0.3653402028812674	0.4981051766720732
9	0.32075120522484496	0.49197655718443656
>10	3.3376731836927123	13.30343644195596
>50	1.300643523057514	16.169968089879074
>100	1.5782373512689927	46.438370762148864
>500	0.01283627138564153	1.4071032373943257
>1k	0.002473043110995057	0.6121330586296613
>5k	2.3552791533286262E-4	0.2688970071973837
>10k+	1.1776395766643131E-4	0.7897319253776571
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	39349	0.782106071384347	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7262	0.14434049887908532	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	6136	0.12195996985982752	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.9876135896321303E-5	0.0	0.0	0.020889818827033692	0.0
2	1.9876135896321303E-5	0.0	0.0	0.0702422642575995	0.0
3	1.9876135896321303E-5	0.0	0.0	0.10828518836315847	0.0
4	3.9752271792642606E-5	0.0	0.0	0.1623284018652561	0.0
5	3.9752271792642606E-5	0.0	0.0	0.26852659595930084	0.0
6	3.9752271792642606E-5	0.0	0.0	0.4569126119846342	0.0
7	3.9752271792642606E-5	0.0	0.0	0.5633493197094347	0.0
8	3.9752271792642606E-5	0.0	0.0	0.8163327773978123	0.0
9	3.9752271792642606E-5	0.0	0.0	0.9512718639979376	0.0
10	3.9752271792642606E-5	0.0	0.0	1.151424552473893	0.0
11	3.9752271792642606E-5	0.0	0.0	1.2974743990400621	0.0
12	3.9752271792642606E-5	0.0	0.0	1.4250394392226522	0.0
13	3.9752271792642606E-5	0.0	0.0	1.4872716207140342	0.0
14	3.9752271792642606E-5	0.0	0.0	1.5176423563636132	0.0
15	3.9752271792642606E-5	0.0	0.0	1.5515112919309448	0.0
16	3.9752271792642606E-5	0.0	0.0	1.6160888574580927	0.0
17	3.9752271792642606E-5	0.0	0.0	1.6912405272820834	0.0
18	3.9752271792642606E-5	0.0	0.0	1.8068600097909846	0.0
19	3.9752271792642606E-5	0.0	0.0	1.8606845857982226	0.0
20	5.962840768896391E-5	0.0	0.0	1.9210086582435577	0.0
21	5.962840768896391E-5	0.0	0.0	1.994888255370184	0.0
22	5.962840768896391E-5	0.0	0.0	2.079163071570586	0.0
23	5.962840768896391E-5	0.0	0.0	2.176317623831805	0.0
24	5.962840768896391E-5	0.0	0.0	2.2536755447402874	0.0
25	5.962840768896391E-5	0.0	0.0	2.3211749022441945	0.0
26	5.962840768896391E-5	0.0	0.0	2.38571271549955	0.0
27	5.962840768896391E-5	0.0	0.0	2.4530331877803904	0.0
28	7.950454358528521E-5	0.0	0.0	2.522500282738033	0.0
29	7.950454358528521E-5	0.0	0.0	2.601905445643837	0.0
30	7.950454358528521E-5	0.0	0.0	2.7127347794017242	0.0
31	7.950454358528521E-5	0.0	0.0	2.803767481806876	0.0
32	7.950454358528521E-5	0.0	0.0	2.894561670581272	0.0
33	7.950454358528521E-5	0.0	0.0	2.9795917799457343	0.0
34	7.950454358528521E-5	0.0	0.0	3.073168627745615	0.0
35	1.1925681537792783E-4	0.0	0.0	3.18320291606765	0.0
36	1.1925681537792783E-4	0.0	0.0	3.2852072454875705	0.0
37	1.3913295127424913E-4	0.0	0.0	3.391186802086756	0.0
38	1.5900908717057043E-4	0.0	0.0	3.4930519985554023	0.0
39	1.7888522306689175E-4	0.0	0.0	3.602390622121066	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCTA	540	0.0	42.5	31
CGTTTTT	28315	0.0	42.409504	1
TACGTAG	425	0.0	41.294117	1
ACGGGAT	1755	0.0	38.71795	5
CGAATAT	895	0.0	38.463688	14
GTTGATC	3275	0.0	38.33588	16
GGCGATA	2290	0.0	38.122272	8
CGCTATA	95	0.0	37.894737	43
GCGTTAG	440	0.0	37.840908	1
CGCATCG	335	0.0	37.61194	21
GGGCGAT	9890	0.0	37.58342	7
TAGTAGG	2360	0.0	37.372883	2
GCTACGA	940	0.0	37.340427	10
ATAGCGG	790	0.0	37.310123	2
AACGGGA	1285	0.0	37.29572	4
CGTATGG	545	0.0	37.15596	2
GGTACCT	3485	0.0	37.058823	8
ACGGGTA	365	0.0	36.9863	5
TATGGGC	1725	0.0	36.913044	4
GTAGCAT	3325	0.0	36.8797	29
>>END_MODULE