FastQCFastQC Report
Sat 18 Jun 2016
SRR3549052_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549052_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2495395
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133930.5367086172730169No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58180.23314946130772884No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATAT453.8562575E-1045.00000416
ATTACGC207.034878E-445.012
GCGATCG351.212702E-745.09
GTACGTC207.034878E-445.027
TACCGTA207.034878E-445.034
CTTACGA253.8921236E-545.036
TACCGGA207.034878E-445.018
CTTACCG302.1665292E-644.9999961
TATAGCG1050.042.857141
CGTTTTT90000.041.51
CGGTCTA3800.041.4473731
ACGTTAG1200.041.2499961
ACGTAGG2750.040.9090922
ACTACGT501.0822987E-940.522
CGTAAGG5300.040.330192
TCGTTAG950.040.2631571
TACGGGA6500.040.1538474
CTATACG2250.040.01
ATACTAT21450.039.96503445
CGACGGT3900.039.80769328
Produced by FastQC (version 0.11.5)