##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549052_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2495395
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.039350483590773	31.0	31.0	33.0	30.0	34.0
2	31.31793603818233	31.0	31.0	34.0	28.0	34.0
3	31.42797513018981	31.0	31.0	34.0	30.0	34.0
4	35.20682857824112	37.0	35.0	37.0	32.0	37.0
5	35.05850696983844	37.0	35.0	37.0	32.0	37.0
6	35.09825418420731	37.0	35.0	37.0	32.0	37.0
7	35.71477060745894	37.0	35.0	37.0	35.0	37.0
8	35.71156550365774	37.0	35.0	37.0	35.0	37.0
9	37.351692617801994	39.0	37.0	39.0	35.0	39.0
10	36.57221802560316	39.0	35.0	39.0	32.0	39.0
11	36.22008219139655	38.0	35.0	39.0	31.0	39.0
12	35.392999505088376	37.0	35.0	39.0	30.0	39.0
13	35.01207343927514	37.0	34.0	39.0	29.0	39.0
14	35.939696921729826	38.0	34.0	40.0	29.0	41.0
15	36.25261251224756	38.0	35.0	40.0	30.0	41.0
16	36.40713955105304	38.0	35.0	40.0	31.0	41.0
17	36.356664576149264	38.0	35.0	40.0	31.0	41.0
18	36.29475373638242	38.0	35.0	40.0	31.0	41.0
19	36.20924663229669	37.0	35.0	40.0	30.0	41.0
20	36.0714143452239	37.0	34.0	40.0	30.0	41.0
21	35.93029600524166	37.0	34.0	40.0	30.0	41.0
22	35.81835180402301	37.0	34.0	40.0	30.0	41.0
23	35.76504361033023	37.0	34.0	40.0	30.0	41.0
24	35.674839454274775	37.0	34.0	40.0	30.0	41.0
25	35.56409826901152	36.0	34.0	40.0	29.0	41.0
26	35.383983697971665	36.0	34.0	40.0	29.0	41.0
27	35.28854029121642	36.0	34.0	40.0	29.0	41.0
28	35.28289789792798	36.0	34.0	40.0	29.0	41.0
29	35.30673781104795	36.0	34.0	40.0	29.0	41.0
30	35.25673731012525	36.0	34.0	40.0	29.0	41.0
31	35.10398273620008	36.0	34.0	40.0	28.0	41.0
32	34.93780303318713	36.0	34.0	40.0	27.0	41.0
33	34.75253937753342	36.0	33.0	40.0	26.0	41.0
34	34.630024905876624	36.0	34.0	40.0	25.0	41.0
35	34.47356230175984	36.0	33.0	40.0	25.0	41.0
36	34.276972182760645	36.0	33.0	40.0	24.0	41.0
37	34.20009377272937	35.0	33.0	40.0	24.0	41.0
38	34.18244887082005	35.0	33.0	40.0	24.0	41.0
39	34.12444362515754	35.0	33.0	40.0	24.0	41.0
40	33.97126987911734	35.0	33.0	40.0	23.0	41.0
41	33.946537121377574	35.0	33.0	40.0	23.0	41.0
42	33.873123894213144	35.0	33.0	40.0	23.0	41.0
43	33.824253074162606	35.0	33.0	40.0	23.0	41.0
44	33.69835396800907	35.0	33.0	39.0	23.0	41.0
45	33.62572738985211	35.0	32.0	39.0	23.0	41.0
46	33.56958036703608	35.0	32.0	39.0	23.0	41.0
47	33.48947040448506	35.0	32.0	39.0	23.0	41.0
48	33.43339511379962	35.0	32.0	39.0	23.0	41.0
49	33.413391066344204	35.0	32.0	39.0	23.0	41.0
50	33.3220183578151	35.0	32.0	39.0	23.0	41.0
51	33.1517342945706	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	2.0
13	16.0
14	24.0
15	102.0
16	378.0
17	843.0
18	1939.0
19	3614.0
20	6024.0
21	9615.0
22	13943.0
23	19325.0
24	26781.0
25	35973.0
26	46354.0
27	54141.0
28	61540.0
29	70119.0
30	83920.0
31	100855.0
32	123118.0
33	155724.0
34	247575.0
35	338040.0
36	187302.0
37	217515.0
38	286037.0
39	404287.0
40	285.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.574965085687836	24.231875113959912	28.09839724773032	14.094762552621928
2	30.702273587949	26.243380306524617	29.3795972180757	13.674748887450683
3	28.728437782395172	25.92234896679684	31.31331913384454	14.035894116963446
4	26.18795821903947	28.23432763149722	30.895589676183533	14.682124473279782
5	23.291422800799072	32.9315799703053	29.374387621999727	14.402609606895902
6	21.95924893654111	41.051216340499195	26.602882509582653	10.38665221337704
7	86.75949098238956	4.598831046788184	6.786781251064461	1.854896719757794
8	87.50646691205199	3.8731743872212614	6.521813179877334	2.0985455208494046
9	82.11513608066058	6.146321524247664	8.366691445642873	3.3718509494488846
10	47.81070732288876	26.206592543465064	13.954985082522006	12.02771505112417
11	42.9767231239944	22.18863145914775	20.568887891496136	14.265757525361716
12	38.94545753277537	21.06179582791502	24.19384506260532	15.798901576704289
13	21.92755054810962	37.75895198956478	24.858509374267403	15.454988088058203
14	17.39027288264984	40.01009860162419	26.88444114058095	15.715187375145018
15	16.027682992071394	23.014152068109457	44.48417985930083	16.473985080518315
16	17.27421911160357	19.459965256001553	43.05013835484963	20.21567727754524
17	17.55221117298063	19.647550788552515	28.796683490990404	34.00355454747645
18	21.218564595985807	22.462255474584182	34.63680098741882	21.682378942011184
19	29.232085501493753	23.45708795601498	26.086811907533676	21.22401463495759
20	30.28250036567357	23.159419650997137	25.655737869154983	20.90234211417431
21	23.21632446967314	27.893539900496716	28.124565449558087	20.76557018027206
22	23.8186739975034	24.415693707809787	24.58276144658461	27.182870848102205
23	20.391521182017275	29.312313281063716	25.00790456019989	25.28826097671912
24	19.678728217376406	24.465385239611365	37.83112493212498	18.024761610887253
25	18.688904962941738	25.595466849937587	34.65872136475387	21.0569068223668
26	18.32828069303657	32.6579158810529	27.54998707619435	21.46381634971618
27	19.21691756214948	32.779259395807074	27.045938618936084	20.957884423107366
28	16.830241304482858	28.49580928069504	35.88377791892666	18.79017149589544
29	16.78006888688965	24.718331166007786	35.26139148311189	23.240208463990673
30	18.87440665706231	30.86705711921359	30.320370121764288	19.93816610195981
31	27.571506715369708	27.11157952949333	25.261812258179567	20.055101496957395
32	28.944555871916066	25.695491094596246	26.497247930688328	18.862705102799357
33	27.369935421045565	26.770270838885224	24.995842341593214	20.863951398475994
34	20.320390158672275	27.286662031461955	27.22130163761649	25.171646172249286
35	20.922178653078973	25.52076925697134	30.462511947006387	23.094540142943302
36	29.157027244183787	26.293111912142166	24.69336517865909	19.856495665014958
37	20.660456560985335	33.27020371524348	27.11354314647581	18.955796577295377
38	21.36110715938759	32.90998018349801	24.69492805748188	21.033984599632525
39	21.060673761067886	31.716181205781048	25.799202130324055	21.423942902827005
40	24.18482845401229	26.570583013911627	25.63626199459404	23.608326537482043
41	19.13228166282292	25.189879758515183	26.743741972713735	28.934096605948156
42	20.831732050436905	26.707795759789533	25.04549379957882	27.41497839019474
43	21.33421762887238	25.78609799250219	27.12203879546124	25.75764558316419
44	20.250741866518126	28.256007565936454	28.979740682336864	22.513509885208556
45	18.374125138505125	35.71001785288501	23.767740177406782	22.148116831203076
46	21.861468825576715	32.19458242081915	26.27291470889378	19.671034044710357
47	21.913484638704496	27.386606128488676	26.902915169742663	23.796994063064165
48	21.88443112212696	25.53022667754003	30.30049350904366	22.284848691289355
49	21.698528689846697	24.668399191310396	32.09924681262886	21.533825306214048
50	20.77378531254571	31.91178150152581	26.840440090646972	20.47399309528151
51	19.787408406284378	32.49938386507948	24.86207594388864	22.851131784747505
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2346.0
1	3187.0
2	4028.0
3	12775.0
4	21522.0
5	15598.5
6	9675.0
7	10049.5
8	10424.0
9	10957.5
10	11491.0
11	11664.5
12	11838.0
13	12058.5
14	12279.0
15	11635.5
16	10992.0
17	10464.0
18	9936.0
19	9878.0
20	9820.0
21	10980.0
22	12140.0
23	11982.0
24	11824.0
25	13043.0
26	16998.0
27	19734.0
28	23771.0
29	27808.0
30	33283.0
31	38758.0
32	42728.0
33	46698.0
34	54955.0
35	63212.0
36	67627.0
37	72042.0
38	83299.0
39	94556.0
40	124812.5
41	155069.0
42	189067.0
43	223065.0
44	240038.0
45	257011.0
46	243446.0
47	229881.0
48	214230.0
49	198579.0
50	186142.5
51	173706.0
52	157107.0
53	140508.0
54	128375.0
55	116242.0
56	109133.5
57	102025.0
58	97197.5
59	92370.0
60	84866.5
61	77363.0
62	67888.0
63	58413.0
64	52629.5
65	46846.0
66	40443.0
67	34040.0
68	28588.5
69	23137.0
70	20830.5
71	18524.0
72	15990.5
73	13457.0
74	11586.5
75	7155.5
76	4595.0
77	3427.0
78	2259.0
79	2110.5
80	1962.0
81	1416.5
82	871.0
83	547.0
84	223.0
85	162.5
86	102.0
87	66.0
88	30.0
89	19.0
90	8.0
91	5.0
92	2.0
93	3.0
94	4.0
95	2.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2495395.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.12114145885759
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57207375830203	13.794858724493963
2	7.094024554442356	2.429155958184335
3	2.5173325257944663	1.2929881881933099
4	1.3529162538973751	0.926538822598586
5	0.8742757535900992	0.7484299425632704
6	0.597412154214764	0.6137026800931091
7	0.4225042548048114	0.506362857943767
8	0.3419444464990888	0.4683583391665327
9	0.27164402921868713	0.41857702656364676
>10	2.982247238599011	12.212899320637115
>50	1.2886489848780316	16.1460325821689
>100	1.6732422293262443	48.119814329533284
>500	0.009152376817792709	1.0193078042420967
>1k	0.0021120869579521633	0.5310895406390489
>5k	2.346763286613515E-4	0.23376297075487867
>10k+	2.346763286613515E-4	0.5381209122241475
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13393	0.5367086172730169	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5818	0.23314946130772884	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02636857090761182	0.0
2	0.0	0.0	0.0	0.09257051488842448	0.0
3	0.0	0.0	0.0	0.1337263238886028	0.0
4	0.0	0.0	0.0	0.21756074689578203	0.0
5	0.0	0.0	0.0	0.3728868575916839	0.0
6	0.0	0.0	0.0	0.5999050250561534	0.0
7	0.0	0.0	0.0	0.7278206456292491	0.0
8	0.0	0.0	0.0	1.095297538065116	0.0
9	4.0073815969014924E-5	0.0	0.0	1.2517056417921812	0.0
10	4.0073815969014924E-5	0.0	0.0	1.503529501341471	0.0
11	8.014763193802985E-5	0.0	0.0	1.6908345171806467	0.0
12	8.014763193802985E-5	0.0	0.0	1.8695236625864844	0.0
13	8.014763193802985E-5	0.0	0.0	1.9508735090035847	0.0
14	8.014763193802985E-5	0.0	0.0	1.9808887971643767	0.0
15	8.014763193802985E-5	0.0	0.0	2.0210026869493607	0.0
16	8.014763193802985E-5	0.0	0.0	2.1059591768036725	0.0
17	1.2022144790704477E-4	0.0	0.0	2.2054624618547365	0.0
18	1.2022144790704477E-4	0.0	0.0	2.361830491765833	0.0
19	2.0036907984507464E-4	0.0	0.0	2.421660699007572	0.0
20	2.0036907984507464E-4	0.0	0.0	2.4931924605122635	0.0
21	2.0036907984507464E-4	0.0	0.0	2.5842401703938656	0.0
22	2.805167117831045E-4	0.0	0.0	2.6777724568655463	0.0
23	2.805167117831045E-4	0.0	0.0	2.7864125719575457	0.0
24	3.205905277521194E-4	0.0	0.0	2.8769793960475196	0.0
25	3.205905277521194E-4	0.0	0.0	2.946186876226008	0.0
26	3.205905277521194E-4	0.0	0.0	3.0167167923314744	0.0
27	3.205905277521194E-4	0.0	0.0	3.091454459113687	0.0
28	3.205905277521194E-4	0.0	0.0	3.167554635638847	0.0
29	3.205905277521194E-4	0.0	0.0	3.249465515479513	0.0
30	3.606643437211343E-4	0.0	0.0	3.369005708515085	0.0
31	3.606643437211343E-4	0.0	0.0	3.4712740868680108	0.0
32	4.808857916281791E-4	0.0	0.0	3.5614000989823253	0.0
33	4.808857916281791E-4	0.0	0.0	3.652047070704237	0.0
34	4.808857916281791E-4	0.0	0.0	3.7444973641447548	0.0
35	4.808857916281791E-4	0.0	0.0	3.8671232410099403	0.0
36	5.20959607597194E-4	0.0	0.0	3.970513686209999	0.0
37	5.61033423566209E-4	0.0	0.0	4.07827217735068	0.0
38	5.61033423566209E-4	0.0	0.0	4.180460408071668	0.0
39	5.61033423566209E-4	0.0	0.0	4.284051222351572	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATAT	45	3.8562575E-10	45.000004	16
ATTACGC	20	7.034878E-4	45.0	12
GCGATCG	35	1.212702E-7	45.0	9
GTACGTC	20	7.034878E-4	45.0	27
TACCGTA	20	7.034878E-4	45.0	34
CTTACGA	25	3.8921236E-5	45.0	36
TACCGGA	20	7.034878E-4	45.0	18
CTTACCG	30	2.1665292E-6	44.999996	1
TATAGCG	105	0.0	42.85714	1
CGTTTTT	9000	0.0	41.5	1
CGGTCTA	380	0.0	41.44737	31
ACGTTAG	120	0.0	41.249996	1
ACGTAGG	275	0.0	40.909092	2
ACTACGT	50	1.0822987E-9	40.5	22
CGTAAGG	530	0.0	40.33019	2
TCGTTAG	95	0.0	40.263157	1
TACGGGA	650	0.0	40.153847	4
CTATACG	225	0.0	40.0	1
ATACTAT	2145	0.0	39.965034	45
CGACGGT	390	0.0	39.807693	28
>>END_MODULE