##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549051_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2342255
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.00035179773338	31.0	31.0	33.0	30.0	34.0
2	31.279392721970922	31.0	31.0	34.0	28.0	34.0
3	31.4030624334242	31.0	31.0	34.0	30.0	34.0
4	35.19132075713362	37.0	35.0	37.0	32.0	37.0
5	35.066598641053176	37.0	35.0	37.0	32.0	37.0
6	35.10912347289258	37.0	35.0	37.0	32.0	37.0
7	35.693276778147556	37.0	35.0	37.0	35.0	37.0
8	35.668864406309304	37.0	35.0	37.0	35.0	37.0
9	37.31898021351219	39.0	37.0	39.0	35.0	39.0
10	36.52503207379213	38.0	35.0	39.0	32.0	39.0
11	36.21763215362973	38.0	35.0	39.0	31.0	39.0
12	35.521693410836995	37.0	35.0	39.0	30.0	39.0
13	35.212641663695884	37.0	34.0	39.0	30.0	39.0
14	36.16077028333807	38.0	35.0	40.0	30.0	41.0
15	36.43183556017598	38.0	35.0	40.0	31.0	41.0
16	36.54588590909188	38.0	35.0	40.0	31.0	41.0
17	36.461589792742465	38.0	35.0	40.0	31.0	41.0
18	36.4083146369631	38.0	35.0	40.0	31.0	41.0
19	36.33773649752055	38.0	35.0	40.0	30.0	41.0
20	36.22666404810749	38.0	34.0	40.0	30.0	41.0
21	36.09592209217186	38.0	34.0	40.0	30.0	41.0
22	35.986673953092215	38.0	34.0	40.0	30.0	41.0
23	35.93416984914111	37.0	34.0	40.0	30.0	41.0
24	35.82277762241942	37.0	34.0	40.0	30.0	41.0
25	35.69581663823965	37.0	34.0	40.0	29.0	41.0
26	35.51871679215115	37.0	34.0	40.0	29.0	41.0
27	35.45272568529046	37.0	34.0	40.0	29.0	41.0
28	35.45511483591667	37.0	34.0	40.0	29.0	41.0
29	35.43370469910407	37.0	34.0	40.0	29.0	41.0
30	35.36081084254276	37.0	34.0	40.0	29.0	41.0
31	35.25806284968972	37.0	34.0	40.0	28.0	41.0
32	35.10147486076452	37.0	34.0	40.0	27.0	41.0
33	34.91604073851908	37.0	33.0	40.0	27.0	41.0
34	34.77229422074027	36.0	33.0	40.0	26.0	41.0
35	34.635060657357975	36.0	33.0	40.0	25.0	41.0
36	34.46507318801753	36.0	33.0	40.0	24.0	41.0
37	34.41496677347257	36.0	33.0	40.0	24.0	41.0
38	34.35657005748734	36.0	33.0	40.0	24.0	41.0
39	34.297363438225126	36.0	33.0	40.0	24.0	41.0
40	34.17051046961155	36.0	33.0	40.0	23.0	41.0
41	34.10600084106982	36.0	33.0	40.0	23.0	41.0
42	34.04008871792354	35.0	33.0	40.0	23.0	41.0
43	33.976727128344265	35.0	33.0	40.0	23.0	41.0
44	33.86450706690775	35.0	33.0	40.0	23.0	41.0
45	33.78313590962555	35.0	33.0	40.0	23.0	41.0
46	33.71226531697018	35.0	32.0	39.0	23.0	41.0
47	33.61394382763619	35.0	32.0	39.0	23.0	41.0
48	33.53754736354496	35.0	32.0	39.0	23.0	41.0
49	33.50262631523895	35.0	32.0	39.0	23.0	41.0
50	33.442565817983095	35.0	32.0	39.0	23.0	41.0
51	33.27419943601358	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	3.0
12	3.0
13	8.0
14	28.0
15	70.0
16	292.0
17	690.0
18	1548.0
19	3070.0
20	5253.0
21	8113.0
22	11920.0
23	16769.0
24	23532.0
25	31583.0
26	40827.0
27	49519.0
28	56463.0
29	65715.0
30	78317.0
31	95271.0
32	115504.0
33	145951.0
34	223442.0
35	289996.0
36	187622.0
37	223177.0
38	286423.0
39	380912.0
40	233.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.01286367197423	23.92327052349125	27.82643222023221	14.237433584302305
2	30.936469342577986	26.52836689429631	29.078217358912674	13.456946404213033
3	29.614751596218174	25.80158864000717	30.764626396357357	13.819033367417296
4	26.274508966786282	28.588774492956574	30.18407474847956	14.952641791777582
5	23.403856539958287	32.658058153360756	29.01084638521425	14.927238921466707
6	21.19542919110003	41.91452254344638	26.68210762705171	10.207940638401881
7	87.42382874622959	4.497460780316405	6.368350158287633	1.7103603151663673
8	87.63418158996352	3.947051025614205	6.3520837825087355	2.0666836019135406
9	82.3819353571665	6.1469822884357175	8.126143396000863	3.3449389583969293
10	46.311695353409426	26.818685412134887	14.291014428403399	12.578604806052288
11	40.628710366719254	23.004412414532148	21.002922397433245	15.363954821315357
12	36.686142200571666	21.664250903509654	25.420844442641815	16.22876245327686
13	22.635537121278425	34.193885806626525	25.61821834087236	17.55235873122269
14	18.016185257369504	35.96922623710911	28.112267878604165	17.902320626917223
15	17.92016667698436	23.16237984335608	41.66139041223095	17.25606306742861
16	19.86884434017645	19.669933461557346	39.82794358428096	20.63327861398524
17	19.39541168660116	20.051104597919526	28.969561384221613	31.583922331257696
18	22.74013717549968	22.331428473842514	33.387611511129236	21.540822839528573
19	29.117324971021514	23.796640417034013	25.300276869939438	21.785757742005032
20	30.377264644541263	22.744833504464715	25.9626727235079	20.915229127486118
21	24.11441111236821	27.23913493620464	27.03676585171128	21.60968809971587
22	24.41890400490126	24.026504372922673	25.31462202023264	26.239969601943425
23	21.970622327628718	28.37786662852678	25.176080315764082	24.47543072808042
24	21.049757605384556	23.53138321830885	36.34736610659386	19.071493069712734
25	21.013382402855367	24.853570597565167	33.03666765574201	21.096379343837455
26	19.499456720126545	31.68536303690247	26.470303190728593	22.34487705224239
27	19.742598478816355	31.141229285453548	27.59212809877661	21.524044136953492
28	18.703087409355515	27.02583621339265	34.27841972799717	19.992656649254673
29	19.81919133484612	24.14075324847209	32.9496574881898	23.09039792849199
30	20.294032887110923	29.786210297341665	29.061780207535048	20.857976608012365
31	28.138481933009	26.77308832727436	24.742566458391593	20.345863281325048
32	28.927764056432796	26.05164681044549	25.870923533090973	19.14966560003074
33	27.866137546936603	26.665883945172492	24.611410798568045	20.856567709322853
34	20.962747437832345	26.904073211499174	27.487997677451858	24.645181673216623
35	22.432613016089196	25.100085174329866	29.748554277822016	22.718747531758922
36	29.11365329564885	26.43922203175999	24.288388753572946	20.158735919018213
37	22.14839972590516	31.865360517962394	26.956202463011074	19.03003729312137
38	22.531406699953678	31.164668236379043	24.579902700602624	21.724022363064655
39	21.750321805268854	30.358351246982075	26.403231074327945	21.488095873421127
40	24.878204977681765	26.29905795910351	25.995504332363474	22.82723273085125
41	19.508806684156934	25.018710601535698	27.63072338408926	27.841759330218103
42	21.87007819387727	25.955670923960028	25.08138524626909	27.092865635893617
43	21.78076255574222	25.94277736625602	27.29467969969111	24.981780378310646
44	21.293795935967687	27.652454579027474	27.698179745587055	23.355569739417785
45	19.414068920762258	33.083075924696494	24.82257482639593	22.680280328145315
46	22.11821513882989	31.086751869459135	25.26778681228133	21.527246179429653
47	22.130639063637393	27.460075867059736	26.974945084971537	23.43433998433134
48	22.163897611489784	24.96436126724033	30.67010210246109	22.201639018808798
49	22.12940094054661	25.54995079528062	30.21285043686533	22.107797827307447
50	21.29499136515879	31.16240545969589	26.45177403826654	21.090829136878778
51	20.077105182825953	30.858254118360296	25.787798510409843	23.276842188403908
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1941.0
1	2722.5
2	3504.0
3	10941.5
4	18379.0
5	13653.5
6	8928.0
7	9254.5
8	9581.0
9	10053.5
10	10526.0
11	10364.5
12	10203.0
13	10365.0
14	10527.0
15	9991.5
16	9456.0
17	8802.0
18	8148.0
19	8511.0
20	8874.0
21	8911.0
22	8948.0
23	9802.5
24	10657.0
25	11998.0
26	15416.0
27	17493.0
28	21647.5
29	25802.0
30	31325.0
31	36848.0
32	43130.0
33	49412.0
34	56711.0
35	64010.0
36	67453.0
37	70896.0
38	79980.0
39	89064.0
40	116597.0
41	144130.0
42	163938.0
43	183746.0
44	192776.5
45	201807.0
46	190468.0
47	179129.0
48	172506.5
49	165884.0
50	164350.0
51	162816.0
52	150098.5
53	137381.0
54	130414.0
55	123447.0
56	118550.0
57	113653.0
58	108815.5
59	103978.0
60	96732.5
61	89487.0
62	81648.5
63	73810.0
64	62650.0
65	51490.0
66	45177.0
67	38864.0
68	33870.0
69	28876.0
70	24859.0
71	20842.0
72	17353.5
73	13865.0
74	11527.0
75	7697.5
76	6206.0
77	4816.5
78	3427.0
79	2706.0
80	1985.0
81	1368.5
82	752.0
83	685.5
84	619.0
85	420.0
86	221.0
87	153.0
88	85.0
89	53.0
90	21.0
91	11.5
92	2.0
93	4.0
94	6.0
95	3.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2342255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.703841820881646
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.65690649525705	13.47280207858415
2	6.387447700175226	2.1338983204576247
3	2.189593743518546	1.0972388263117767
4	1.1083735511061745	0.7405638590450567
5	0.7851085193476739	0.655716425970507
6	0.5493635962011707	0.5505889567857031
7	0.4071574204105943	0.4760765202715742
8	0.32262450979988	0.4311255023389341
9	0.26669028674404177	0.4009277128444238
>10	3.877804554031888	16.3998008448059
>50	1.908976095228567	23.29063879568075
>100	1.5307414432741284	38.526621561435284
>500	0.0076767374208714925	0.8604728045221379
>1k	0.0010235649894495323	0.27894518515282263
>5k	2.558912473623831E-4	0.23966375316455354
>10k+	2.558912473623831E-4	0.44491885262882785
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10409	0.44440080179143604	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5607	0.23938469551778094	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2748	0.11732283632653148	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017632580568725437	0.0
2	0.0	0.0	0.0	0.05883219376199432	0.0
3	0.0	0.0	0.0	0.08538779936428784	0.0
4	4.2693899682143915E-5	0.0	0.0	0.13171068051941398	0.0
5	4.2693899682143915E-5	0.0	0.0	0.22367334043475198	0.0
6	4.2693899682143915E-5	0.0	0.0	0.3590130024271482	0.0
7	4.2693899682143915E-5	0.0	0.0	0.43692936934706084	0.0
8	4.2693899682143915E-5	0.0	0.0	0.6378895551509123	0.0
9	4.2693899682143915E-5	0.0	0.0	0.7257109067970823	0.0
10	4.2693899682143915E-5	0.0	0.0	0.8651918770586465	0.0
11	4.2693899682143915E-5	0.0	0.0	0.9771352820252278	0.0
12	4.2693899682143915E-5	0.0	0.0	1.0788748449677767	0.0
13	4.2693899682143915E-5	0.0	0.0	1.1236607457343457	0.0
14	4.2693899682143915E-5	0.0	0.0	1.1424033676948069	0.0
15	4.2693899682143915E-5	0.0	0.0	1.1694713000932861	0.0
16	4.2693899682143915E-5	0.0	0.0	1.2297123925447913	0.0
17	4.2693899682143915E-5	0.0	0.0	1.2981934076349502	0.0
18	4.2693899682143915E-5	0.0	0.0	1.4068920762256885	0.0
19	4.2693899682143915E-5	0.0	0.0	1.447067035826586	0.0
20	4.2693899682143915E-5	0.0	0.0	1.4967200411569193	0.0
21	4.2693899682143915E-5	0.0	0.0	1.5542714179284494	0.0
22	4.2693899682143915E-5	0.0	0.0	1.617373001658658	0.0
23	4.2693899682143915E-5	0.0	0.0	1.6899526311183026	0.0
24	4.2693899682143915E-5	0.0	0.0	1.7524991941526435	0.0
25	4.2693899682143915E-5	0.0	0.0	1.8050126907616806	0.0
26	4.2693899682143915E-5	0.0	0.0	1.8568430849758033	0.0
27	4.2693899682143915E-5	0.0	0.0	1.9052579672153545	0.0
28	8.538779936428783E-5	0.0	0.0	1.9554232993418736	0.0
29	8.538779936428783E-5	0.0	0.0	2.0123769615178535	0.0
30	8.538779936428783E-5	0.0	0.0	2.0903360223374485	0.0
31	8.538779936428783E-5	0.0	0.0	2.156725036343182	0.0
32	8.538779936428783E-5	0.0	0.0	2.223669071044784	0.0
33	8.538779936428783E-5	0.0	0.0	2.2886064924613247	0.0
34	8.538779936428783E-5	0.0	0.0	2.357258283150212	0.0
35	8.538779936428783E-5	0.0	0.0	2.4452077164954287	0.0
36	8.538779936428783E-5	0.0	0.0	2.521971348123923	0.0
37	1.2808169904643174E-4	0.0	0.0	2.5952767738781644	0.0
38	1.2808169904643174E-4	0.0	0.0	2.671698854309202	0.0
39	1.2808169904643174E-4	0.0	0.0	2.750597180921804	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGTAC	25	3.8920494E-5	45.000004	13
CCCGATA	30	2.166471E-6	45.000004	24
TGCGCTA	20	7.034789E-4	45.0	35
TATACGA	20	7.034789E-4	45.0	18
CGACGGT	275	0.0	42.545456	28
ACGGGTA	415	0.0	41.20482	5
CGTTTTT	7565	0.0	40.925312	1
TATTCCG	55	6.184564E-11	40.909092	1
TACGATC	50	1.0822987E-9	40.500004	11
TACGGGT	240	0.0	40.312504	4
GCGATCG	45	1.9297659E-8	40.0	9
TAGTGCG	130	0.0	39.80769	1
TCGATAG	80	0.0	39.375	1
CGTAAGG	470	0.0	39.255318	2
ATGTACG	115	0.0	39.130432	1
TATTGCG	105	0.0	38.57143	1
GTTAGTC	35	6.251068E-6	38.571426	13
TCGTAAG	100	0.0	38.250004	1
TTACGGG	365	0.0	38.219177	3
CGGGTAC	265	0.0	38.207546	6
>>END_MODULE