##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549049_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1745736
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14000455968142	31.0	31.0	33.0	30.0	34.0
2	31.422471095285886	31.0	31.0	34.0	30.0	34.0
3	31.524905254860986	31.0	31.0	34.0	30.0	34.0
4	35.30796580926326	37.0	35.0	37.0	33.0	37.0
5	35.16849569465257	37.0	35.0	37.0	32.0	37.0
6	35.21956699065609	37.0	35.0	37.0	32.0	37.0
7	35.779804048263884	37.0	35.0	37.0	35.0	37.0
8	35.78127162411728	37.0	35.0	37.0	35.0	37.0
9	37.488560125929695	39.0	37.0	39.0	35.0	39.0
10	36.7257391724751	39.0	37.0	39.0	32.0	39.0
11	36.36208911313051	38.0	35.0	39.0	32.0	39.0
12	35.47405678750968	37.0	35.0	39.0	30.0	39.0
13	35.078846973425534	37.0	34.0	39.0	30.0	39.0
14	36.041470760756496	38.0	35.0	40.0	30.0	41.0
15	36.400531924643815	38.0	35.0	40.0	31.0	41.0
16	36.5756133802591	38.0	35.0	40.0	31.0	41.0
17	36.52617807045281	38.0	35.0	40.0	31.0	41.0
18	36.43603099208586	38.0	35.0	40.0	31.0	41.0
19	36.33453511871211	37.0	35.0	40.0	31.0	41.0
20	36.18473870046788	37.0	34.0	40.0	31.0	41.0
21	36.056937589647	37.0	34.0	40.0	30.0	41.0
22	35.95872457232938	37.0	34.0	40.0	30.0	41.0
23	35.90104574803979	37.0	34.0	40.0	30.0	41.0
24	35.80849108914521	36.0	34.0	40.0	30.0	41.0
25	35.68265247437184	36.0	34.0	40.0	30.0	41.0
26	35.514016437766074	36.0	34.0	40.0	30.0	41.0
27	35.46407761540118	36.0	34.0	40.0	29.0	41.0
28	35.49183610809423	36.0	34.0	40.0	29.0	41.0
29	35.5251263650403	36.0	34.0	40.0	30.0	41.0
30	35.46074549645537	36.0	34.0	40.0	29.0	41.0
31	35.284394089369755	36.0	34.0	40.0	29.0	41.0
32	35.10633509304958	36.0	34.0	40.0	29.0	41.0
33	34.905806490786695	36.0	34.0	40.0	27.0	41.0
34	34.72830026991481	36.0	34.0	40.0	26.0	41.0
35	34.574514130429804	36.0	33.0	40.0	26.0	41.0
36	34.35222622435466	35.0	33.0	40.0	24.0	41.0
37	34.291351040478055	35.0	33.0	40.0	24.0	41.0
38	34.28628039978553	35.0	33.0	40.0	24.0	41.0
39	34.23414651470784	35.0	33.0	40.0	24.0	41.0
40	34.06990461329777	35.0	33.0	40.0	23.0	41.0
41	34.03850066676748	35.0	33.0	40.0	23.0	41.0
42	33.95919428825435	35.0	33.0	40.0	23.0	41.0
43	33.895493934936326	35.0	33.0	40.0	23.0	41.0
44	33.79358791936467	35.0	33.0	40.0	23.0	41.0
45	33.71851413959499	35.0	33.0	39.0	23.0	41.0
46	33.659846620565766	35.0	33.0	39.0	23.0	41.0
47	33.56061798576646	35.0	33.0	39.0	23.0	41.0
48	33.54260151592222	35.0	33.0	39.0	23.0	41.0
49	33.5151517755262	35.0	32.0	39.0	24.0	41.0
50	33.424041779513054	35.0	32.0	39.0	23.0	41.0
51	33.243219478775714	35.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	0.0
13	2.0
14	15.0
15	54.0
16	179.0
17	446.0
18	1140.0
19	2147.0
20	3709.0
21	5847.0
22	8755.0
23	12465.0
24	17496.0
25	23897.0
26	30526.0
27	36125.0
28	40243.0
29	46153.0
30	55113.0
31	67566.0
32	84684.0
33	108293.0
34	176406.0
35	252582.0
36	129705.0
37	153086.0
38	201091.0
39	287814.0
40	193.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.515131726675737	23.962729759826228	29.072608916812165	15.449529596685869
2	30.035755692727882	26.21261175802069	30.133823212673622	13.61780933657781
3	27.45804634835966	26.071926110248057	32.48400674557894	13.986020795813342
4	25.069426305008317	28.844624845910268	31.545033155070413	14.540915694011009
5	21.72264305714037	32.550397081803894	31.06535008729842	14.661609773757315
6	20.978200598486826	41.66615112479779	27.05832955269296	10.297318724022418
7	88.02722748456812	4.277565450904374	6.257475357098668	1.4377317074288438
8	88.84361667514446	3.2799919346338737	6.160324356030923	1.7160670341907367
9	84.4822470293332	5.079118492143142	7.8615552408840745	2.5770792376395972
10	50.14274781524812	24.862579450730237	13.596729402383867	11.397943331637773
11	43.55658587552757	20.552935839095944	21.1877970093989	14.702681275977584
12	40.126055715182595	19.899916138522663	25.76145534032637	14.212572805968371
13	21.252583437587354	40.384399473918165	23.53030469670099	14.832712391793489
14	15.080287053712588	40.99915451133504	26.642172699652182	17.278385735300184
15	12.85486465307469	22.750347131525043	47.116975304398835	17.277812911001433
16	14.96560762910314	18.43491799447339	45.27843843513566	21.3210359412878
17	15.91603770558664	18.916090405422125	29.002781634794722	36.165090254196514
18	19.878549792179346	23.06963939564745	34.38297657836007	22.668834233813133
19	28.173618462356277	24.82637695504933	24.740396027807183	22.259608554787206
20	30.564988062341612	22.678343117172357	25.048690065393618	21.70797875509241
21	21.013142880710486	28.688415659641546	27.22536511820802	23.073076341439943
22	22.734193486300335	24.792408474133545	23.426107956758642	29.047290082807482
23	18.98752159547606	30.608465426616625	24.308887483559943	26.09512549434737
24	19.8850227067552	23.243548852747494	39.51256089122296	17.358867549274347
25	17.702733975813068	25.983653885810913	34.73652373554764	21.577088402828377
26	17.00841364330002	33.85752484911808	26.635298808067198	22.498762699514703
27	16.563501010462062	33.935829930757	28.364598083559027	21.136070975221912
28	14.712190159336808	28.013800483005447	37.96123812535229	19.312771232305455
29	15.422606854644688	23.507620854470552	36.89990926463108	24.169863026253683
30	17.4430154387605	31.604778729429878	30.415996462237132	20.53620936957249
31	27.545287489059056	28.110264094914694	24.196556638575366	20.147891777450884
32	29.31027371836291	25.972942071424317	25.825783509075826	18.891000701136942
33	27.370289665791393	27.0252203082253	24.410334666868298	21.194155359115012
34	19.15381248940275	26.677286829165464	28.22820861802701	25.940692063404775
35	19.949751852513785	25.905406086601868	30.59219721653217	23.55264484435218
36	29.376492207298238	25.637438879647323	25.791757745730166	19.19431116732427
37	19.821897469033118	32.95572755559832	29.00163598619723	18.220738989171327
38	20.351989075094977	32.10594270840494	26.35197990990619	21.190088306593896
39	20.895198357598172	32.04785832451184	26.32047457347503	20.736468744414964
40	24.28700559534775	26.268462127148663	26.18826672532387	23.256265552179713
41	17.603749936989328	24.014054816994093	28.14165486648611	30.24054037953047
42	20.22081231068157	26.252537611643454	24.864469770916106	28.662180306758867
43	20.19050990527777	27.543168039153688	26.108644147797833	26.157677907770704
44	19.856324209387903	29.47593450556098	28.64700046284203	22.02074082220909
45	17.304334676033488	37.271614952088974	23.164441817090328	22.259608554787206
46	20.860084228084887	32.89936164460148	25.806593895067753	20.433960232245884
47	20.679930986128486	28.02336664879455	28.21577833074417	23.0809240343328
48	22.56887639368152	23.86494865202986	30.89178432477763	22.67439062951099
49	20.103154199718627	25.452359348721686	32.115050614755035	22.32943583680465
50	19.511827676120557	33.01782170958266	27.156282507778958	20.314068106517823
51	18.58093090822438	33.385002085080444	24.97164519721195	23.062421809483222
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2286.0
1	2518.5
2	2751.0
3	8625.0
4	14499.0
5	10565.5
6	6632.0
7	7315.5
8	7999.0
9	8702.5
10	9406.0
11	9827.5
12	10249.0
13	10220.0
14	10191.0
15	9650.0
16	9109.0
17	8629.5
18	8150.0
19	8118.5
20	8087.0
21	7939.5
22	7792.0
23	7947.5
24	8103.0
25	8770.5
26	11348.0
27	13258.0
28	16001.5
29	18745.0
30	23028.0
31	27311.0
32	31830.0
33	36349.0
34	43354.5
35	50360.0
36	54229.5
37	58099.0
38	63382.5
39	68666.0
40	94418.0
41	120170.0
42	142267.0
43	164364.0
44	163353.0
45	162342.0
46	151511.0
47	140680.0
48	134924.5
49	129169.0
50	124343.0
51	119517.0
52	115320.0
53	111123.0
54	97599.5
55	84076.0
56	77858.5
57	71641.0
58	66471.5
59	61302.0
60	55298.5
61	49295.0
62	46770.0
63	44245.0
64	38714.5
65	33184.0
66	27961.0
67	22738.0
68	19394.5
69	16051.0
70	13708.0
71	11365.0
72	9140.5
73	6916.0
74	5864.5
75	3535.0
76	2257.0
77	1753.0
78	1249.0
79	1112.5
80	976.0
81	792.0
82	608.0
83	357.0
84	106.0
85	70.0
86	34.0
87	24.0
88	14.0
89	10.5
90	7.0
91	8.0
92	9.0
93	7.0
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1745736.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.596109968550827
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4656593255527	14.15882590184373
2	6.778611093976024	2.385543724872815
3	2.471111525059264	1.3044585041848846
4	1.2860651025815228	0.9051897188696031
5	0.8402287291185904	0.739237855815321
6	0.60262844932259	0.6362349878674534
7	0.4277949640641976	0.5269269062166109
8	0.353082022727404	0.4970296079863812
9	0.2764127492751828	0.43774102196600256
>10	3.3952742628727646	14.367804802183493
>50	1.507673741829589	19.3984094785941
>100	1.590889325812779	43.28782869997818
>500	0.002937026447654124	0.2903281058079053
>1k	0.0013053450878462774	0.5468343654289324
>5k	3.2633627196156935E-4	0.5176063183845844
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9014	0.5163438228918921	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4220	0.24173185407186426	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2400	0.13747783169963843	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1842	0.1055142358294725	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.018616789709326037	0.0
2	0.0	0.0	0.0	0.07733128033104662	0.0
3	0.0	0.0	0.0	0.11891832442018724	0.0
4	0.0	0.0	0.0	0.18473583634638915	0.0
5	0.0	0.0	0.0	0.29294234637997957	0.0
6	0.0	0.0	0.0	0.4759597098301232	0.0
7	0.0	0.0	0.0	0.5641746518373912	0.0
8	0.0	0.0	0.0	0.8256116617862036	0.0
9	0.0	0.0	0.0	0.9579913572269805	0.0
10	0.0	0.0	0.0	1.167244073559805	0.0
11	0.0	0.0	0.0	1.3394350577636023	0.0
12	0.0	0.0	0.0	1.4924937103891998	0.0
13	0.0	0.0	0.0	1.5652423963302584	0.0
14	0.0	0.0	0.0	1.598065228648547	0.0
15	0.0	0.0	0.0	1.6350123959178249	0.0
16	0.0	0.0	0.0	1.7147495383036153	0.0
17	0.0	0.0	0.0	1.8035373046096317	0.0
18	0.0	0.0	0.0	1.948290004903376	0.0
19	0.0	0.0	0.0	2.004140374031354	0.0
20	0.0	0.0	0.0	2.069556908948432	0.0
21	5.7282429874849345E-5	0.0	0.0	2.1472318838587277	0.0
22	5.7282429874849345E-5	0.0	0.0	2.2318953152137553	0.0
23	5.7282429874849345E-5	0.0	0.0	2.3332852160922384	0.0
24	5.7282429874849345E-5	0.0	0.0	2.4130796409079034	0.0
25	5.7282429874849345E-5	0.0	0.0	2.4772932447976097	0.0
26	5.7282429874849345E-5	0.0	0.0	2.5392728339221966	0.0
27	5.7282429874849345E-5	0.0	0.0	2.6036010026716525	0.0
28	1.7184728962454804E-4	0.0	0.0	2.669189384878355	0.0
29	1.7184728962454804E-4	0.0	0.0	2.748410985395272	0.0
30	1.7184728962454804E-4	0.0	0.0	2.8591952047732305	0.0
31	2.2912971949939738E-4	0.0	0.0	2.9427129875307605	0.0
32	2.2912971949939738E-4	0.0	0.0	3.0184403598253113	0.0
33	2.2912971949939738E-4	0.0	0.0	3.0939958848302376	0.0
34	2.2912971949939738E-4	0.0	0.0	3.17860203375539	0.0
35	2.2912971949939738E-4	0.0	0.0	3.293510588084338	0.0
36	2.2912971949939738E-4	0.0	0.0	3.3842459570060996	0.0
37	2.2912971949939738E-4	0.0	0.0	3.4756687150863588	0.0
38	2.2912971949939738E-4	0.0	0.0	3.5635972449442526	0.0
39	2.2912971949939738E-4	0.0	0.0	3.6588006433962525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAATGCG	30	2.1661508E-6	45.000004	1
CTTCGCA	55	1.8189894E-12	45.0	26
TCCGCGT	20	7.034294E-4	45.0	32
GTCGATA	20	7.034294E-4	45.0	38
CGACGAA	25	3.8916412E-5	45.0	11
ATAGCCG	25	3.8916412E-5	45.0	1
CGTTCGC	20	7.034294E-4	45.0	42
CGCTAGC	20	7.034294E-4	45.0	35
TATTACG	20	7.034294E-4	45.0	1
TCCGTAC	20	7.034294E-4	45.0	43
ACTCGAT	25	3.8916412E-5	45.0	33
ACGTATA	70	0.0	45.0	13
ATCGGCG	20	7.034294E-4	45.0	10
CGGTAAG	25	3.8916412E-5	45.0	1
CGATCGA	25	3.8916412E-5	45.0	10
CGATATC	20	7.034294E-4	45.0	10
TATCCGT	25	3.8916412E-5	45.0	30
CGTTTTT	6615	0.0	42.959183	1
TGCGAAG	145	0.0	41.89655	1
TAGCACG	70	0.0	41.785713	1
>>END_MODULE