##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549048_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4354041
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.082372674028562	31.0	31.0	33.0	30.0	34.0
2	31.36840856574387	31.0	31.0	34.0	30.0	34.0
3	31.452540065653952	31.0	31.0	34.0	30.0	34.0
4	35.23367441877557	37.0	35.0	37.0	33.0	37.0
5	35.102454708166505	37.0	35.0	37.0	32.0	37.0
6	35.14786769348291	37.0	35.0	37.0	32.0	37.0
7	35.74416065443573	37.0	35.0	37.0	35.0	37.0
8	35.728968790142304	37.0	35.0	37.0	35.0	37.0
9	37.36415573486791	39.0	37.0	39.0	35.0	39.0
10	36.62729220969669	39.0	35.0	39.0	32.0	39.0
11	36.298132470502686	38.0	35.0	39.0	32.0	39.0
12	35.580915981268895	37.0	35.0	39.0	30.0	39.0
13	35.2183941308775	37.0	35.0	39.0	30.0	39.0
14	36.19119824549195	38.0	35.0	40.0	30.0	41.0
15	36.49524338424925	38.0	35.0	40.0	31.0	41.0
16	36.62537468067021	38.0	35.0	40.0	31.0	41.0
17	36.55693205461318	38.0	35.0	40.0	31.0	41.0
18	36.45385907022924	38.0	35.0	40.0	31.0	41.0
19	36.37773277743595	38.0	35.0	40.0	31.0	41.0
20	36.24665385557922	38.0	35.0	40.0	30.0	41.0
21	36.08495969606166	37.0	34.0	40.0	30.0	41.0
22	35.975767338892766	37.0	34.0	40.0	30.0	41.0
23	35.91996859928513	37.0	34.0	40.0	30.0	41.0
24	35.807502501699	37.0	34.0	40.0	30.0	41.0
25	35.6946333302787	37.0	34.0	40.0	30.0	41.0
26	35.51125104242243	37.0	34.0	40.0	29.0	41.0
27	35.399183654908164	37.0	34.0	40.0	29.0	41.0
28	35.40200815747946	37.0	34.0	40.0	29.0	41.0
29	35.371382814263804	36.0	34.0	40.0	29.0	41.0
30	35.30520084675363	36.0	34.0	40.0	29.0	41.0
31	35.158637688528884	36.0	34.0	40.0	27.0	41.0
32	34.983308379503086	36.0	34.0	40.0	27.0	41.0
33	34.75638079659792	36.0	33.0	40.0	26.0	41.0
34	34.59887171480471	36.0	33.0	40.0	25.0	41.0
35	34.42069516570928	36.0	33.0	40.0	24.0	41.0
36	34.25234764670337	36.0	33.0	40.0	23.0	41.0
37	34.17860603517514	36.0	33.0	40.0	23.0	41.0
38	34.12323701131891	36.0	33.0	40.0	23.0	41.0
39	34.061586925800654	36.0	33.0	40.0	23.0	41.0
40	33.870604112363665	35.0	33.0	40.0	23.0	41.0
41	33.77977331862516	35.0	33.0	40.0	23.0	41.0
42	33.740216272653385	35.0	33.0	40.0	23.0	41.0
43	33.69420591124429	35.0	33.0	40.0	23.0	41.0
44	33.58298693099123	35.0	32.0	40.0	23.0	41.0
45	33.499914906635006	35.0	32.0	39.0	23.0	41.0
46	33.42513563836445	35.0	32.0	39.0	23.0	41.0
47	33.3218100151101	35.0	32.0	39.0	23.0	41.0
48	33.229744735982045	35.0	32.0	39.0	23.0	41.0
49	33.18310438509881	35.0	32.0	39.0	22.0	41.0
50	33.10136147087269	35.0	32.0	39.0	22.0	41.0
51	32.95560606801819	35.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	3.0
11	5.0
12	11.0
13	19.0
14	62.0
15	195.0
16	662.0
17	1587.0
18	3533.0
19	6645.0
20	11293.0
21	17231.0
22	25474.0
23	35358.0
24	48972.0
25	65129.0
26	82368.0
27	95982.0
28	106996.0
29	122306.0
30	143163.0
31	171185.0
32	208683.0
33	262479.0
34	412764.0
35	557263.0
36	337102.0
37	401317.0
38	520830.0
39	715028.0
40	394.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.15848426783303	23.960293437751275	27.95931411762085	14.921908176794844
2	32.57534782056485	26.03705385410932	28.218613467351368	13.168984857974467
3	29.431992027635935	25.692362566177028	31.132550198769373	13.74309520741766
4	26.249592045642196	29.00546871285778	29.546368534425838	15.198570707074188
5	23.173943469985698	32.22112515706673	29.702637159365285	14.902294213582278
6	21.761623282830822	41.41607761617311	26.28842953017668	10.533869570819384
7	85.92399566287962	4.652298864434211	7.519405536144469	1.904299936541709
8	86.39753277472583	4.215348454458743	7.21993660601726	2.1671821647981724
9	81.13159706121279	6.255981512346806	9.211259149833454	3.4011622766069496
10	46.81469467099644	26.67395644643677	14.743682937298939	11.767665945267856
11	41.247866062813834	23.618197440033292	21.218059269538344	13.915877227614532
12	38.218748973654584	20.870841592901858	24.73102572989092	16.17938370355263
13	23.19750778644482	34.37560647683382	25.498221077844697	16.928664658876663
14	16.98465861942963	37.779961190076065	28.054765676299326	17.180614514194975
15	16.241693635866085	24.051702774503042	43.57331499634478	16.1332885932861
16	18.128354785818505	19.630155067441947	43.315049169265976	18.92644097747357
17	18.26705352567879	18.97756589797845	30.288805273078502	32.46657530326426
18	21.613439101744795	23.24550917182452	33.061677646122305	22.079374080308384
19	29.100231256435116	23.941023982089284	26.15064947711792	20.80809528435768
20	31.446855920741214	22.818067170244838	25.26099317852083	20.47408373049312
21	24.86425828328213	26.483191132100043	27.70520534831895	20.94734523629888
22	23.411102467799452	24.84149781777434	24.82700553348028	26.920394180945927
23	21.956568622114492	29.754405160631237	25.04723772697593	23.241788490278342
24	21.778435251298735	23.487720947046665	35.74665465943017	18.98718914222443
25	20.563655693641838	24.234797054047032	33.005339178018765	22.196208074292365
26	20.02413390227607	31.528504210226778	26.975790995077908	21.471570892419248
27	20.64638344011919	32.256310861565154	27.416416152259476	19.680889546056182
28	18.972536087740103	28.35735814155172	33.947682164683336	18.72242360602484
29	19.58991658553514	25.486324083764945	33.008141172763416	21.9156181579365
30	21.034046303192827	28.79871824817451	29.318534207647563	20.848701240985097
31	27.742090623400195	26.96789028858479	24.746941978727346	20.543077109287672
32	30.21576967235724	26.309145917551074	25.688113639719973	17.786970770371706
33	28.906273505463087	27.356196232419492	24.36570992326439	19.371820338853034
34	22.549810624199452	27.827046185371245	26.06514270306596	23.558000487363348
35	23.297805418001346	25.21747039129857	28.859443445755335	22.625280744944753
36	29.668140469968012	25.863651720321425	24.022419632704423	20.44578817700614
37	23.141307121361514	32.216554690229145	26.325843050168796	18.316295138240545
38	23.772789461559963	30.767142523462688	23.64137131460177	21.818696700375583
39	22.82233906387193	29.629142215243263	25.66565174742268	21.88286697346212
40	25.78887061467726	25.628077457240295	26.367298792087627	22.215753135994813
41	19.66968156707757	26.14463207856793	27.8804448557099	26.305241498644595
42	23.52527227005901	26.922047817188677	24.18932205737153	25.36335785538078
43	23.430463792141598	26.742421580320443	25.449898152084465	24.37721647545349
44	21.285651650960567	29.08406236872827	27.756238400143683	21.87404758016748
45	20.178266580401978	33.55861830423738	23.774466064972746	22.488649050387906
46	22.359252014393068	31.475013671207964	24.529167272425774	21.636567041973194
47	23.02757369533268	28.55338293782718	25.602239390947396	22.81680397589274
48	24.355650302787687	24.682817639980883	29.103676331940836	21.857855725290598
49	22.41370717455348	25.649207253675378	29.313274725708833	22.623810846062316
50	21.035768840945686	32.22879619185947	25.59879431544168	21.136640651753165
51	20.307479879036507	32.37661289822489	24.272830687630183	23.04307653510842
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4484.0
1	6307.5
2	8131.0
3	22860.0
4	37589.0
5	28374.0
6	19159.0
7	20255.5
8	21352.0
9	22608.0
10	23864.0
11	24455.5
12	25047.0
13	24877.5
14	24708.0
15	23737.5
16	22767.0
17	22857.0
18	22947.0
19	21766.5
20	20586.0
21	21291.0
22	21996.0
23	21476.5
24	20957.0
25	24135.5
26	33182.5
27	39051.0
28	42881.0
29	46711.0
30	54144.0
31	61577.0
32	69533.0
33	77489.0
34	89197.0
35	100905.0
36	109285.5
37	117666.0
38	134709.5
39	151753.0
40	198944.5
41	246136.0
42	307291.0
43	368446.0
44	373030.0
45	377614.0
46	360405.0
47	343196.0
48	317688.0
49	292180.0
50	286434.5
51	280689.0
52	269455.0
53	258221.0
54	242056.0
55	225891.0
56	211581.5
57	197272.0
58	201285.5
59	205299.0
60	194019.5
61	182740.0
62	161677.0
63	140614.0
64	118912.0
65	97210.0
66	82992.0
67	68774.0
68	61307.0
69	53840.0
70	48002.5
71	42165.0
72	37033.5
73	31902.0
74	25930.0
75	15776.0
76	11594.0
77	9410.0
78	7226.0
79	5409.5
80	3593.0
81	2713.0
82	1833.0
83	1464.0
84	1095.0
85	697.5
86	300.0
87	200.0
88	100.0
89	66.0
90	32.0
91	27.0
92	22.0
93	18.5
94	15.0
95	10.0
96	5.0
97	15.5
98	26.0
99	13.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4354041.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.384547872914919
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.96634883910596	12.610152176832697
2	6.809717204416349	2.0952884066471134
3	2.378873707324092	1.0979368930183828
4	1.2676022429192246	0.7800594956002055
5	0.8067825411851822	0.6205992313947694
6	0.5709951606162137	0.527070143022173
7	0.4161139715178784	0.44812077207838885
8	0.3414409453755856	0.42023316559232193
9	0.2785467577900747	0.3856784337059977
>10	2.3665881745201274	8.212733545543193
>50	0.9393799073471369	10.63684666959228
>100	1.8253449944789613	57.95172997144691
>500	0.028213600185243423	2.7765108364148974
>1k	0.003751808535271732	0.7547451250012721
>5k	1.5007234141086926E-4	0.18592727108091908
>10k+	1.5007234141086926E-4	0.49636786302852093
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	21499	0.49377118864980835	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	8053	0.18495462031708015	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.012517107670782153	0.0
2	0.0	0.0	0.0	0.053329768828543414	0.0
3	0.0	0.0	0.0	0.09078922316073734	0.0
4	0.0	0.0	0.0	0.15041199657972903	0.0
5	0.0	0.0	0.0	0.24790763339160105	0.0
6	0.0	0.0	0.0	0.4691733495389685	0.0
7	0.0	0.0	0.0	0.5822866619767706	0.0
8	0.0	0.0	0.0	0.8680901259312901	0.0
9	0.0	0.0	0.0	1.0293885611090938	0.0
10	0.0	0.0	0.0	1.2709802227402085	0.0
11	0.0	0.0	0.0	1.4508820656488994	0.0
12	0.0	0.0	0.0	1.5995485573057304	0.0
13	0.0	0.0	0.0	1.6769479203342366	0.0
14	0.0	0.0	0.0	1.709446465938194	0.0
15	0.0	0.0	0.0	1.7438972209953925	0.0
16	0.0	0.0	0.0	1.8150495137735267	0.0
17	0.0	0.0	0.0	1.882733763875903	0.0
18	0.0	0.0	0.0	2.001244361272666	0.0
19	2.2967170038132394E-5	0.0	0.0	2.044767148494927	0.0
20	2.2967170038132394E-5	0.0	0.0	2.097752409772898	0.0
21	2.2967170038132394E-5	0.0	0.0	2.1630710413613468	0.0
22	2.2967170038132394E-5	0.0	0.0	2.2298136374921596	0.0
23	2.2967170038132394E-5	0.0	0.0	2.31244951528936	0.0
24	2.2967170038132394E-5	0.0	0.0	2.372853172489648	0.0
25	2.2967170038132394E-5	0.0	0.0	2.422462259772014	0.0
26	2.2967170038132394E-5	0.0	0.0	2.4726684934753713	0.0
27	2.2967170038132394E-5	0.0	0.0	2.525676721923381	0.0
28	4.593434007626479E-5	0.0	0.0	2.577352854509179	0.0
29	4.593434007626479E-5	0.0	0.0	2.6350234184749293	0.0
30	4.593434007626479E-5	0.0	0.0	2.7202316193164005	0.0
31	4.593434007626479E-5	0.0	0.0	2.7883522456495013	0.0
32	4.593434007626479E-5	0.0	0.0	2.854359892339094	0.0
33	6.890151011439717E-5	0.0	0.0	2.917887084664568	0.0
34	6.890151011439717E-5	0.0	0.0	2.983136814742902	0.0
35	6.890151011439717E-5	0.0	0.0	3.06813831105403	0.0
36	6.890151011439717E-5	0.0	0.0	3.1414954521558247	0.0
37	6.890151011439717E-5	0.0	0.0	3.218711077824026	0.0
38	1.1483585019066197E-4	0.0	0.0	3.290345681172961	0.0
39	1.1483585019066197E-4	0.0	0.0	3.3634501834043364	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAAACG	40	6.8230293E-9	45.0	36
CACTACG	25	3.892605E-5	45.0	1
ACGTTAG	260	0.0	42.40385	1
CGTAAGG	655	0.0	41.564884	2
CGTTTTT	17415	0.0	41.07235	1
ACGTAAG	285	0.0	41.05263	1
CGTTGAT	700	0.0	40.82143	25
ACGGGAT	1380	0.0	39.94565	5
ACGGGTA	360	0.0	39.375004	5
TTACGAG	115	0.0	39.130436	1
CATACGA	1065	0.0	39.084507	18
GCGATCG	180	0.0	38.750004	9
ACATACG	1080	0.0	38.75	17
GGCGATA	1850	0.0	38.554054	8
GACCGAT	2555	0.0	38.131115	9
AACGGGA	1140	0.0	38.092106	4
TCGAATC	65	9.094947E-12	38.076927	44
CACGACG	350	0.0	37.928574	26
CTAGTCG	190	0.0	37.894737	1
TCGTTGA	790	0.0	37.879745	24
>>END_MODULE