##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549047_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1316598
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.05693537435117	31.0	31.0	33.0	30.0	34.0
2	31.341174754936585	31.0	31.0	34.0	30.0	34.0
3	31.43557486795514	31.0	31.0	34.0	30.0	34.0
4	35.209765623219845	37.0	35.0	37.0	32.0	37.0
5	35.069273992517076	37.0	35.0	37.0	32.0	37.0
6	35.1150784066207	37.0	35.0	37.0	32.0	37.0
7	35.71568770421951	37.0	35.0	37.0	35.0	37.0
8	35.70882835914987	37.0	35.0	37.0	35.0	37.0
9	37.35472634775383	39.0	37.0	39.0	35.0	39.0
10	36.61158987025652	39.0	35.0	39.0	32.0	39.0
11	36.2543669366048	38.0	35.0	39.0	31.0	39.0
12	35.38694954724221	37.0	35.0	39.0	30.0	39.0
13	34.96987691003632	37.0	34.0	39.0	28.0	39.0
14	35.88793845957536	38.0	34.0	40.0	29.0	41.0
15	36.236643227469585	38.0	35.0	40.0	30.0	41.0
16	36.41324231086482	38.0	35.0	40.0	31.0	41.0
17	36.35550183123474	38.0	35.0	40.0	31.0	41.0
18	36.27136832958883	38.0	35.0	40.0	31.0	41.0
19	36.16994253371188	37.0	35.0	40.0	30.0	41.0
20	36.02298347711298	37.0	34.0	40.0	30.0	41.0
21	35.85405112266614	37.0	34.0	40.0	30.0	41.0
22	35.74571281438981	37.0	34.0	40.0	30.0	41.0
23	35.69236167759635	36.0	34.0	40.0	30.0	41.0
24	35.58247544049133	36.0	34.0	40.0	29.0	41.0
25	35.45644912114404	36.0	34.0	40.0	29.0	41.0
26	35.27456064797303	36.0	34.0	40.0	29.0	41.0
27	35.165087596973414	36.0	34.0	40.0	29.0	41.0
28	35.17492051484204	36.0	34.0	40.0	28.0	41.0
29	35.18475723037708	36.0	34.0	40.0	29.0	41.0
30	35.10376591791876	36.0	34.0	40.0	28.0	41.0
31	34.923124598396775	36.0	34.0	40.0	27.0	41.0
32	34.719420810300484	35.0	33.0	40.0	26.0	41.0
33	34.50357132549191	35.0	33.0	40.0	25.0	41.0
34	34.34324220453016	35.0	33.0	40.0	24.0	41.0
35	34.17579245904977	35.0	33.0	40.0	24.0	41.0
36	33.95352947520807	35.0	33.0	40.0	23.0	41.0
37	33.88022995629645	35.0	33.0	40.0	23.0	41.0
38	33.84308118347438	35.0	33.0	40.0	23.0	41.0
39	33.77651112944118	35.0	33.0	40.0	23.0	41.0
40	33.60649568053422	35.0	33.0	40.0	23.0	41.0
41	33.55587734448936	35.0	33.0	40.0	23.0	41.0
42	33.49237884304852	35.0	33.0	39.0	23.0	41.0
43	33.40653942965127	35.0	32.0	39.0	23.0	41.0
44	33.274836358554396	35.0	32.0	39.0	22.0	41.0
45	33.20032386499144	35.0	32.0	39.0	22.0	41.0
46	33.128282133194794	35.0	32.0	39.0	22.0	41.0
47	33.037901470304526	35.0	32.0	39.0	22.0	41.0
48	32.96804111809375	35.0	32.0	39.0	22.0	41.0
49	32.954684725329976	35.0	32.0	39.0	21.0	40.0
50	32.86176570221131	35.0	32.0	39.0	20.0	40.0
51	32.68868781511137	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	3.0
13	4.0
14	23.0
15	86.0
16	240.0
17	602.0
18	1285.0
19	2367.0
20	3961.0
21	5992.0
22	8341.0
23	11921.0
24	16089.0
25	20930.0
26	25987.0
27	29636.0
28	33420.0
29	37624.0
30	44915.0
31	53418.0
32	65100.0
33	82240.0
34	132130.0
35	182097.0
36	97003.0
37	114495.0
38	148482.0
39	198088.0
40	119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.944906493857655	23.894309424744684	28.724181564911994	15.43660251648567
2	31.268618059574756	26.243773725920892	29.02564032453338	13.461967889970971
3	28.45371176319575	26.608501607931956	31.004908104068214	13.93287852480408
4	26.266407817724165	28.726004444788767	29.99602004560238	15.011567691884691
5	23.1896144457154	32.68894529689396	29.920446484044483	14.200993773346154
6	21.338252070867494	41.802053474181186	26.308334054889954	10.55136040006137
7	85.41004923294734	5.4422078721067475	7.497353026512268	1.6503898684336449
8	86.33090738403067	4.495297729451207	6.828811831705653	2.344983054812479
9	81.05131558759773	6.424132499061977	9.214429917104537	3.3101219962357527
10	50.47736666773002	23.228578503081426	14.980274920666748	11.313779908521813
11	44.941508341954034	22.173662727727066	19.69750827511511	13.187320655203791
12	39.97059087132139	21.20373872662726	23.932969668797917	14.892700733253431
13	22.034668137123102	38.51790751618945	23.985073651942354	15.462350694745092
14	16.76844412645318	41.086725029204054	27.066272316986655	15.078558527356108
15	15.387004993171796	23.600977671240575	45.70081376395832	15.311203571629306
16	16.993265977921887	18.675252430886268	45.350820827617845	18.980660763573997
17	17.20692269014536	19.431291859778003	29.43092728380265	33.930858166273985
18	21.304832606459982	23.372206246705524	33.64337481904119	21.6795863277933
19	29.87115277404341	24.509607336483878	25.741418413213445	19.877821476259268
20	32.05929220612518	23.028061716636362	24.54165964098381	20.37098643625465
21	23.004516184894705	28.323755618647457	27.166226896896394	21.505501299561445
22	23.29260715875309	25.752735459115083	24.409197036604947	26.545460345526884
23	20.715890499605802	31.006730983944987	23.56733034684847	24.710048169600743
24	20.482258062066023	24.519861035790726	36.83151577018953	18.166365131953718
25	18.744445912875456	25.448390473022137	34.57942363576429	21.22773997833811
26	18.56451247837229	33.514937741056876	26.794967028660228	21.125582751910606
27	19.380630989869346	33.79011664912145	27.27187797642105	19.557374384588158
28	17.386856124648524	29.066427261776184	35.306676753268654	18.240039860306638
29	18.039599027189773	25.430237627582603	35.21393773953781	21.316225605689816
30	19.94739472488945	30.66691579358316	30.01219810450874	19.373491377018652
31	29.378519487345418	26.966013923764127	24.153765993872085	19.501700595018374
32	30.955310580754336	25.881020630443007	25.455606039201033	17.708062749601623
33	28.716130512122913	27.562171596797203	23.725768989471348	19.99592890160854
34	20.98385384149148	27.649366017569527	27.26367501697557	24.103105123963427
35	21.228195698307303	25.95439154548313	30.041895855834504	22.775516900375056
36	29.844265295861	26.372362710561614	24.99107548393663	18.792296509640753
37	21.776578727903278	32.64268972002084	27.64298593800082	17.937745614075062
38	21.697815126561032	32.03612644102452	23.844332134789813	22.421726297624637
39	21.5290468313031	32.517290775164476	25.750532812597314	20.203129580935105
40	25.207542469303462	26.35709609159364	25.37031045163368	23.06505098746922
41	18.974736403974486	24.820636215458325	27.455760983990558	28.748866396576634
42	22.19454989298176	26.247115672361648	23.964110533359463	27.594223901297134
43	22.545606175917023	26.08700605651839	26.22212702738421	25.145260740180376
44	20.76184226316613	29.515159524775214	28.213623292759067	21.50937491929959
45	18.41290963528731	35.344121744070705	23.999960504269335	22.24300811637265
46	22.325113664155648	31.933589447956017	25.410034042281698	20.33126284560663
47	22.401142945682736	27.498294847781935	26.376464190284356	23.724098016250974
48	23.39985325816992	24.41177944976371	29.991234985925846	22.197132306140524
49	21.573327621643053	24.54765995391152	30.635774929021615	23.243237495423813
50	20.135531118838095	32.281683551091525	26.850564864901816	20.732220465168563
51	19.580160383047826	32.971871444434825	24.446490120750603	23.00147805176675
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2088.0
1	2468.0
2	2848.0
3	7319.5
4	11791.0
5	8809.0
6	5827.0
7	6182.0
8	6537.0
9	7190.5
10	7844.0
11	8007.0
12	8170.0
13	8166.0
14	8162.0
15	8098.5
16	8035.0
17	7638.5
18	7242.0
19	7075.5
20	6909.0
21	6780.5
22	6652.0
23	6592.0
24	6532.0
25	7599.5
26	9073.5
27	9480.0
28	12156.5
29	14833.0
30	16211.0
31	17589.0
32	20034.5
33	22480.0
34	24469.5
35	26459.0
36	30171.0
37	33883.0
38	41075.5
39	48268.0
40	67816.0
41	87364.0
42	102186.0
43	117008.0
44	122636.5
45	128265.0
46	121419.0
47	114573.0
48	104998.5
49	95424.0
50	89579.5
51	83735.0
52	79067.5
53	74400.0
54	67413.0
55	60426.0
56	58787.5
57	57149.0
58	55194.0
59	53239.0
60	49631.5
61	46024.0
62	41553.5
63	37083.0
64	32166.5
65	27250.0
66	23136.0
67	19022.0
68	17248.5
69	15475.0
70	12689.5
71	9904.0
72	8769.0
73	7634.0
74	6381.0
75	4188.5
76	3249.0
77	2712.5
78	2176.0
79	1508.0
80	840.0
81	676.5
82	513.0
83	388.5
84	264.0
85	165.5
86	67.0
87	56.0
88	45.0
89	32.5
90	20.0
91	19.0
92	18.0
93	12.5
94	7.0
95	3.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1316598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.257873468541867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.20220404472718	16.449839749048017
2	6.080616665228292	2.463607260298035
3	2.186568838961118	1.328857046097925
4	1.18350569629099	0.9590123457904564
5	0.7554827519897661	0.7652236998737239
6	0.5358787888584928	0.6513458819502487
7	0.40363418958747665	0.5723739238168378
8	0.35331848747716527	0.5725984970727206
9	0.26685328731305263	0.4865292116147041
>10	4.084714693841431	20.969941213792712
>50	2.0927535692167814	30.46382077528569
>100	0.8514616165602009	23.209880941102675
>500	0.0018796062175721875	0.22929863970594946
>1k	7.51842487028875E-4	0.34825064077559176
>5k	3.759212435144375E-4	0.5294201737747462
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6952	0.5280275376386718	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2999	0.22778403126846616	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1574	0.11955053858505026	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.024608878336439825	0.0
2	0.0	0.0	0.0	0.08886539399269937	0.0
3	0.0	0.0	0.0	0.13983007721415344	0.0
4	0.0	0.0	0.0	0.22580924473529507	0.0
5	0.0	0.0	0.0	0.354398229376013	0.0
6	0.0	0.0	0.0	0.7146448650233405	0.0
7	0.0	0.0	0.0	0.8717923010668405	0.0
8	0.0	0.0	0.0	1.3777174201996358	0.0
9	0.0	0.0	0.0	1.6356549227630606	0.0
10	0.0	0.0	0.0	1.9671912003512082	0.0
11	0.0	0.0	0.0	2.1860886922204044	0.0
12	0.0	0.0	0.0	2.378782285860984	0.0
13	0.0	0.0	0.0	2.4826104855088644	0.0
14	0.0	0.0	0.0	2.5297015489921755	0.0
15	0.0	0.0	0.0	2.569501092968393	0.0
16	0.0	0.0	0.0	2.6459101411364743	0.0
17	0.0	0.0	0.0	2.720876076068777	0.0
18	0.0	0.0	0.0	2.8452116743303573	7.595332819888835E-5
19	0.0	0.0	0.0	2.892378691141867	7.595332819888835E-5
20	0.0	0.0	0.0	2.951698240465199	7.595332819888835E-5
21	0.0	0.0	0.0	3.0202840958287953	7.595332819888835E-5
22	0.0	0.0	0.0	3.089173764505187	7.595332819888835E-5
23	0.0	0.0	0.0	3.1709754989753898	7.595332819888835E-5
24	0.0	0.0	0.0	3.2350801079752514	7.595332819888835E-5
25	0.0	0.0	0.0	3.2864245578377	7.595332819888835E-5
26	7.595332819888835E-5	0.0	0.0	3.3411109541408996	7.595332819888835E-5
27	7.595332819888835E-5	0.0	0.0	3.3974683236644747	7.595332819888835E-5
28	7.595332819888835E-5	0.0	0.0	3.4519268599830775	7.595332819888835E-5
29	7.595332819888835E-5	0.0	0.0	3.5110945026500118	7.595332819888835E-5
30	7.595332819888835E-5	0.0	0.0	3.6080109494317933	7.595332819888835E-5
31	7.595332819888835E-5	0.0	0.0	3.6792551712823505	7.595332819888835E-5
32	7.595332819888835E-5	0.0	0.0	3.745410520143582	7.595332819888835E-5
33	7.595332819888835E-5	0.0	0.0	3.8167306953223386	7.595332819888835E-5
34	7.595332819888835E-5	0.0	0.0	3.88562036399873	7.595332819888835E-5
35	7.595332819888835E-5	0.0	0.0	3.9737262247094405	7.595332819888835E-5
36	7.595332819888835E-5	0.0	0.0	4.051730292769699	7.595332819888835E-5
37	7.595332819888835E-5	0.0	0.0	4.1294305475171615	7.595332819888835E-5
38	7.595332819888835E-5	0.0	0.0	4.20401671580847	7.595332819888835E-5
39	7.595332819888835E-5	0.0	0.0	4.281413157243137	7.595332819888835E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGTTAG	55	1.8189894E-12	45.000004	1
ACGTTAC	25	3.8911152E-5	45.000004	45
GCGATAC	50	2.1827873E-11	45.000004	9
TTACGAG	45	3.8562575E-10	45.000004	1
CTTAACG	20	7.0336607E-4	45.0	1
AACGCAT	20	7.0336607E-4	45.0	42
ACGTGCG	35	1.2121018E-7	45.0	15
TATCGCC	20	7.0336607E-4	45.0	32
CCGTTGT	20	7.0336607E-4	45.0	29
TCGAATA	30	2.1657434E-6	44.999996	12
AATGCGG	195	0.0	41.538464	2
CGTTTTT	5260	0.0	41.44962	1
CGTTAGG	280	0.0	40.98214	2
CGTTCGA	55	6.184564E-11	40.909092	14
TGCGTAG	155	0.0	40.64516	1
TACGTTG	45	1.9286745E-8	40.000004	1
TCGAGTA	40	3.4587356E-7	39.375	24
AGGGTAC	645	0.0	39.069767	6
AGGGCGA	1400	0.0	39.053574	6
TCGTTAG	75	0.0	39.0	1
>>END_MODULE