##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549041_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1773899
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.953523284020115	31.0	31.0	33.0	30.0	34.0
2	31.238838287861935	31.0	31.0	34.0	28.0	34.0
3	31.330368301690232	31.0	31.0	34.0	28.0	34.0
4	35.156037068626794	35.0	35.0	37.0	32.0	37.0
5	35.01422967147509	35.0	35.0	37.0	32.0	37.0
6	35.06768874665356	37.0	35.0	37.0	32.0	37.0
7	35.57920039415998	37.0	35.0	37.0	35.0	37.0
8	35.509811437968004	37.0	35.0	37.0	35.0	37.0
9	37.20722882193406	39.0	37.0	39.0	34.0	39.0
10	36.634759363413586	39.0	35.0	39.0	32.0	39.0
11	36.28033726835631	38.0	35.0	39.0	32.0	39.0
12	35.21799268165775	37.0	35.0	39.0	30.0	39.0
13	34.60757968745684	37.0	33.0	39.0	27.0	39.0
14	35.534290847449604	37.0	33.0	40.0	27.0	41.0
15	35.9752900249676	37.0	34.0	40.0	30.0	41.0
16	36.14948878149207	37.0	34.0	40.0	31.0	41.0
17	36.09878860070388	37.0	34.0	40.0	31.0	41.0
18	36.0472546633151	37.0	35.0	40.0	31.0	41.0
19	35.922941497796664	36.0	34.0	40.0	30.0	41.0
20	35.77404406902535	36.0	34.0	40.0	30.0	41.0
21	35.56910793681038	36.0	34.0	40.0	30.0	41.0
22	35.463302589380795	35.0	34.0	40.0	30.0	41.0
23	35.43696963581354	35.0	34.0	40.0	30.0	41.0
24	35.329629815451725	35.0	34.0	40.0	29.0	41.0
25	35.20963087526404	35.0	34.0	40.0	29.0	41.0
26	35.06255880408073	35.0	34.0	40.0	29.0	41.0
27	34.923376133590466	35.0	34.0	40.0	29.0	41.0
28	35.0066446849567	36.0	34.0	40.0	29.0	41.0
29	35.08970240132048	36.0	34.0	40.0	29.0	41.0
30	35.007464348308446	36.0	34.0	40.0	29.0	41.0
31	34.81651266503899	35.0	34.0	40.0	28.0	41.0
32	34.55624756539127	35.0	33.0	40.0	27.0	41.0
33	34.283630578742084	35.0	33.0	40.0	25.0	41.0
34	34.120407644403656	35.0	33.0	40.0	24.0	41.0
35	33.93043403260276	35.0	33.0	40.0	23.0	41.0
36	33.69560273724716	35.0	33.0	40.0	23.0	41.0
37	33.62697481649181	35.0	33.0	40.0	23.0	41.0
38	33.6514807212812	35.0	33.0	40.0	23.0	41.0
39	33.6352779949704	35.0	33.0	40.0	23.0	41.0
40	33.44342434377605	35.0	32.0	39.0	23.0	41.0
41	33.435168518613516	35.0	32.0	39.0	23.0	41.0
42	33.36791215283395	35.0	32.0	39.0	23.0	41.0
43	33.260069485354016	35.0	32.0	39.0	23.0	41.0
44	33.211801799313264	35.0	32.0	39.0	22.0	41.0
45	33.18894649582643	35.0	32.0	39.0	23.0	41.0
46	33.12046401739896	35.0	32.0	39.0	23.0	41.0
47	33.0470325537136	35.0	32.0	39.0	22.0	41.0
48	33.02714923453928	35.0	32.0	39.0	22.0	41.0
49	33.06813296585657	35.0	32.0	39.0	22.0	41.0
50	32.92915718425908	35.0	32.0	39.0	21.0	40.0
51	32.74947784513098	35.0	32.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	2.0
12	1.0
13	7.0
14	22.0
15	81.0
16	245.0
17	662.0
18	1434.0
19	2759.0
20	4769.0
21	7477.0
22	11094.0
23	15594.0
24	21722.0
25	29248.0
26	37816.0
27	43846.0
28	47701.0
29	52627.0
30	61204.0
31	73580.0
32	91327.0
33	116562.0
34	195998.0
35	265345.0
36	119237.0
37	136852.0
38	182087.0
39	254493.0
40	106.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.14935010392362	23.340167619464243	28.413342586020963	16.097139690591177
2	31.459175522394457	26.439273036401733	29.015462548882432	13.08608889232138
3	28.595370987863454	25.115296868649228	32.79820327989361	13.4911288635937
4	25.480368386249726	27.99832459457951	31.93338515890702	14.587921860263748
5	22.915509845825497	32.49931365878215	30.992745359234092	13.59243113615826
6	21.57856788915265	40.50236231036829	28.026962076194867	9.89210772428419
7	84.59185105803655	5.433905763518667	8.202834546949967	1.7714086314948034
8	85.65983745410534	4.676421825594355	7.872714286439081	1.791026433861229
9	81.17880443024096	6.155987460390924	9.851068183701553	2.8141399256665682
10	53.15866348647808	21.451728649714553	14.870181447759991	10.519426416047363
11	48.930745211536845	20.24619214509958	19.93591517893634	10.887147464427231
12	44.03728735401508	21.21321450657563	22.96427248676503	11.785225652644261
13	21.134856043100537	43.13312088230503	22.888845419045843	12.843177655548596
14	14.780942996190877	44.75215330748819	27.48792349508061	12.97898020124032
15	13.395238398578499	22.55303148600907	51.18724346763823	12.864486647774198
16	14.796445569899976	17.605511925989024	49.38144730900688	18.21659519510412
17	15.560694267260988	17.8900264332975	30.397897512767074	36.15138178667444
18	20.47348806217265	22.163268596464626	37.53911581211782	19.8241275292449
19	31.047709029657266	22.003563900763233	27.403476748112492	19.545250321467005
20	33.003175490825576	21.447387929076005	26.288024289996216	19.261412290102196
21	22.18733986546021	28.51921107120529	29.185539875720096	20.1079091876144
22	23.228210850786883	25.251043041345646	24.66301632731063	26.857729780556845
23	19.06376856855999	31.166317811780715	25.365029237854014	24.404884381805278
24	18.366885600589438	23.54282853758867	42.64171748222418	15.44856837959771
25	16.37590415237846	24.246589010986533	39.29806601165004	20.079440824984964
26	15.820291910644293	34.75135844825438	29.21502295226504	20.213326688836286
27	17.188351760725947	37.00385422168906	28.779146952560435	17.02864706502456
28	13.983095993627598	29.60416573886112	39.611105254583265	16.80163301292802
29	14.301490671114871	24.64198920006156	40.12426863085215	20.932251497971418
30	17.228094722416554	32.409173239288144	32.62891517499023	17.73381686330507
31	29.14342924822665	27.59988026375797	25.74537783718239	17.511312650833
32	31.14901130222183	25.527045226362944	28.42348972517601	14.900453746239217
33	29.001143808074755	26.939245131769056	25.656872234552246	18.402738825603937
34	19.156389399847455	27.89110315750784	28.749551130024876	24.20295631261983
35	19.990371492401763	25.031019240667028	32.50314702246295	22.475462244468257
36	31.514082819822324	25.693852919472864	25.813532788507125	16.978531472197684
37	19.440114685221648	33.93840348294914	29.648925897133942	16.97255593469527
38	19.700557923534543	34.89285466647199	24.16930163442225	21.237285775571213
39	20.022729591707307	33.00108968999926	27.30459851434608	19.67158220394735
40	24.12589442803677	26.086096220810767	26.104304698294552	23.683704652857916
41	16.86708206047808	24.23300311911783	28.858745621932254	30.04116919847184
42	20.707717857668335	25.693176443529197	25.49846411774289	28.100641581059577
43	21.43143437140446	26.543844942694033	26.679140131428003	25.345580554473507
44	18.19810485264381	30.487756067284554	29.308714870463316	22.005424209608325
45	16.065570813219917	38.96112461870715	23.545308949382125	21.427995618690804
46	20.95598452899517	35.22528621979041	25.100639889869715	18.7180893613447
47	21.34399985568513	28.560588849759768	26.854065535861967	23.24134575869314
48	23.067491441169988	24.14207347768954	32.07629070200727	20.7141443791332
49	21.104696490611925	24.257187134104026	33.42896072437044	21.20915565091361
50	19.813303914146182	34.8270673809501	26.265249599892666	19.094379105011054
51	18.326691654936386	35.18323196529227	23.210227865284324	23.279848514487014
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1964.0
1	2676.5
2	3389.0
3	10687.0
4	17985.0
5	13567.0
6	9149.0
7	9567.0
8	9985.0
9	10807.5
10	11630.0
11	11959.5
12	12289.0
13	12076.5
14	11864.0
15	11251.0
16	10638.0
17	10143.0
18	9648.0
19	9379.5
20	9111.0
21	8315.0
22	7519.0
23	8444.5
24	9370.0
25	10123.0
26	11940.5
27	13005.0
28	17664.5
29	22324.0
30	26042.5
31	29761.0
32	33364.0
33	36967.0
34	41043.0
35	45119.0
36	47357.5
37	49596.0
38	61819.0
39	74042.0
40	101587.0
41	129132.0
42	156602.0
43	184072.0
44	185149.5
45	186227.0
46	175195.0
47	164163.0
48	151286.0
49	138409.0
50	128057.5
51	117706.0
52	109065.5
53	100425.0
54	92582.5
55	84740.0
56	78823.0
57	72906.0
58	65056.0
59	57206.0
60	53374.5
61	49543.0
62	41955.0
63	34367.0
64	27143.0
65	19919.0
66	16168.5
67	12418.0
68	9650.0
69	6882.0
70	5424.0
71	3966.0
72	3331.5
73	2697.0
74	2099.5
75	1091.0
76	680.0
77	478.0
78	276.0
79	241.0
80	206.0
81	175.0
82	144.0
83	88.5
84	33.0
85	29.5
86	26.0
87	20.5
88	15.0
89	10.0
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1773899.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.418163696116732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.91743933795412	13.6135209231336
2	6.320330514553603	2.075364420030055
3	2.004832554379853	0.9874700718333684
4	1.038044308859039	0.6817112554668023
5	0.620243689515813	0.5091631212977011
6	0.40992777393918484	0.40381567776709626
7	0.3064003728563872	0.3521372034675157
8	0.2620603685559863	0.34420400233734927
9	0.21177957256025148	0.31293285208090565
>10	2.841306219366552	11.380394253825802
>50	1.2537953075282855	15.045043471730434
>100	1.8007112334095865	51.79352345471324
>500	0.011401279873851939	1.152227713669143
>1k	0.0010364799885319944	0.24209658257962402
>5k	6.909866590213297E-4	1.1063949960673773
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9978	0.5624897471614787	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9527	0.5370655262785536	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.011894702009528163	0.0
2	0.0	0.0	0.0	0.043463579380787744	0.0
3	0.0	0.0	0.0	0.06060096995375723	0.0
4	0.0	0.0	0.0	0.10040030463966663	0.0
5	0.0	0.0	0.0	0.15829537081874448	0.0
6	0.0	0.0	0.0	0.24054357096993684	0.0
7	0.0	0.0	0.0	0.2866003081347923	0.0
8	0.0	0.0	0.0	0.42708181243689747	0.0
9	5.637299530582068E-5	0.0	0.0	0.48227097484129594	0.0
10	5.637299530582068E-5	0.0	0.0	0.5796835107297541	0.0
11	5.637299530582068E-5	0.0	0.0	0.6602405210217718	0.0
12	5.637299530582068E-5	0.0	0.0	0.7414740072574594	0.0
13	5.637299530582068E-5	0.0	0.0	0.7684766720089475	0.0
14	5.637299530582068E-5	0.0	0.0	0.779018422131136	0.0
15	5.637299530582068E-5	0.0	0.0	0.7957612017369647	0.0
16	5.637299530582068E-5	0.0	0.0	0.839055662131835	0.0
17	1.1274599061164136E-4	0.0	0.0	0.8825192415126227	0.0
18	1.1274599061164136E-4	0.0	0.0	0.9603139750346553	0.0
19	1.1274599061164136E-4	0.0	0.0	0.9875421317673667	0.0
20	1.1274599061164136E-4	0.0	0.0	1.0205203340212718	0.0
21	1.1274599061164136E-4	0.0	0.0	1.0617853665851325	0.0
22	1.6911898591746204E-4	0.0	0.0	1.0998371384165615	0.0
23	1.6911898591746204E-4	0.0	0.0	1.1454992646142763	0.0
24	1.6911898591746204E-4	0.0	0.0	1.1808451326710259	0.0
25	1.6911898591746204E-4	0.0	0.0	1.2137105889343192	0.0
26	1.6911898591746204E-4	0.0	0.0	1.2479289970849523	0.0
27	1.6911898591746204E-4	0.0	0.0	1.2790468904937655	0.0
28	1.6911898591746204E-4	0.0	0.0	1.312814314681952	0.0
29	1.6911898591746204E-4	0.0	0.0	1.3527263953584732	0.0
30	1.6911898591746204E-4	0.0	0.0	1.402109139246372	0.0
31	1.6911898591746204E-4	0.0	0.0	1.4516610021201883	0.0
32	1.6911898591746204E-4	0.0	0.0	1.4946735975385295	0.0
33	1.6911898591746204E-4	0.0	0.0	1.5381371769193173	0.0
34	1.6911898591746204E-4	0.0	0.0	1.5873508018212987	0.0
35	1.6911898591746204E-4	0.0	0.0	1.648910112695255	0.0
36	1.6911898591746204E-4	0.0	0.0	1.7026899502170079	0.0
37	1.6911898591746204E-4	0.0	0.0	1.7541584949312221	0.0
38	1.6911898591746204E-4	0.0	0.0	1.8070927375233878	0.0
39	1.6911898591746204E-4	0.0	0.0	1.8647059387259364	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGATCG	40	6.8193913E-9	45.000004	20
AACCGTA	20	7.034323E-4	45.000004	10
ACTATCG	20	7.034323E-4	45.000004	1
CGACGGT	130	0.0	45.000004	28
CCACTCG	20	7.034323E-4	45.000004	34
CTACGTT	20	7.034323E-4	45.000004	41
CGTAGAT	40	6.8193913E-9	45.000004	31
ACTACGT	20	7.034323E-4	45.000004	31
CGAATCG	20	7.034323E-4	45.000004	12
CCGAATC	20	7.034323E-4	45.000004	11
CGGTCTA	130	0.0	45.000004	31
GAACCGT	20	7.034323E-4	45.000004	9
AGGATCG	40	6.8193913E-9	45.000004	18
TATACGC	20	7.034323E-4	45.000004	20
CGCCGAT	20	7.034323E-4	45.000004	21
ACGTTCA	25	3.891666E-5	45.0	13
ATTAACG	25	3.891666E-5	45.0	1
GGGCGTA	45	3.8562575E-10	45.0	7
AACGCCA	50	2.1827873E-11	45.0	11
CGCATCC	30	2.1661726E-6	44.999996	26
>>END_MODULE