FastQCFastQC Report
Sat 18 Jun 2016
SRR3549039_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549039_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1269621
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82330.6484612337067519No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC33490.26377950585253396No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG19020.14980848615452957No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15330.12074469467660033No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG15280.12035087636389127No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC207.033566E-445.09
ATAATCG207.033566E-445.044
CGACACT207.033566E-445.018
TCGATAG406.8157533E-945.01
ATTCACG207.033566E-445.039
CGTAGCA207.033566E-445.028
TCATCGG502.1827873E-1145.044
CTCGAGG253.8910373E-545.02
TATAGCG351.2120654E-745.01
TACTAGC351.2120654E-745.036
TTACGAC253.8910373E-545.014
ATAACGG502.1827873E-1145.02
ACCGTCC207.033566E-445.027
ACTCGAC302.1656815E-644.99999634
ACGTAAG600.044.9999961
GGCCTAT4400.041.9318168
ACGTATG700.041.7857131
TCGATGG1250.041.3999982
GTCGATG603.6379788E-1241.2499961
CGTTGAT4450.040.9550625
Produced by FastQC (version 0.11.5)