##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549036_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	882984
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.932330597156913	31.0	31.0	33.0	28.0	34.0
2	31.222487610194523	31.0	31.0	34.0	28.0	34.0
3	31.29728851258913	31.0	31.0	34.0	28.0	34.0
4	35.13546225073161	35.0	35.0	37.0	32.0	37.0
5	34.99619925162857	35.0	35.0	37.0	32.0	37.0
6	35.03798030315385	37.0	35.0	37.0	32.0	37.0
7	35.56538397071748	37.0	35.0	37.0	35.0	37.0
8	35.497431437036234	37.0	35.0	37.0	35.0	37.0
9	37.205707011678584	39.0	37.0	39.0	34.0	39.0
10	36.61336332255171	39.0	35.0	39.0	32.0	39.0
11	36.2497610375726	38.0	35.0	39.0	32.0	39.0
12	35.060460891703585	35.0	34.0	39.0	30.0	39.0
13	34.37672709811277	35.0	33.0	39.0	25.0	39.0
14	35.25564676143622	36.0	33.0	40.0	26.0	41.0
15	35.771989073414694	37.0	33.0	40.0	30.0	41.0
16	36.00072707999239	36.0	34.0	40.0	31.0	41.0
17	35.9603424297609	36.0	34.0	40.0	31.0	41.0
18	35.908783171608995	36.0	34.0	40.0	30.0	41.0
19	35.77820889166735	36.0	34.0	40.0	30.0	41.0
20	35.60729526242831	36.0	34.0	40.0	30.0	41.0
21	35.38591865764272	35.0	34.0	40.0	29.0	41.0
22	35.26040335951728	35.0	34.0	40.0	29.0	41.0
23	35.25561844835241	35.0	34.0	40.0	29.0	41.0
24	35.148616509472426	35.0	34.0	40.0	29.0	41.0
25	35.01650086524784	35.0	34.0	40.0	29.0	41.0
26	34.82083367309034	35.0	34.0	39.0	29.0	41.0
27	34.69469775216765	35.0	33.0	39.0	27.0	41.0
28	34.7886247089415	35.0	34.0	40.0	27.0	41.0
29	34.91138571027334	35.0	34.0	40.0	29.0	41.0
30	34.84569482572731	36.0	34.0	40.0	29.0	41.0
31	34.59785228271407	35.0	33.0	39.0	27.0	41.0
32	34.32721317713571	35.0	33.0	40.0	26.0	41.0
33	34.055285259982064	35.0	33.0	40.0	24.0	41.0
34	33.88940682956883	35.0	33.0	39.0	23.0	41.0
35	33.69516208674223	35.0	33.0	39.0	23.0	41.0
36	33.437745191305844	35.0	33.0	39.0	22.0	41.0
37	33.358097088961976	35.0	32.0	39.0	23.0	41.0
38	33.39798003134825	35.0	32.0	39.0	23.0	41.0
39	33.386574388663895	35.0	32.0	39.0	23.0	41.0
40	33.17287062959238	35.0	32.0	39.0	22.0	41.0
41	33.16662929339603	35.0	32.0	39.0	22.0	41.0
42	33.15197104364292	35.0	32.0	39.0	22.0	41.0
43	33.020742165203444	35.0	32.0	39.0	22.0	41.0
44	32.970095720873765	35.0	32.0	39.0	22.0	41.0
45	32.92772462468176	35.0	32.0	39.0	22.0	41.0
46	32.88932642041079	35.0	32.0	38.0	22.0	40.0
47	32.819160936098505	35.0	32.0	38.0	22.0	40.0
48	32.824335435296675	35.0	32.0	38.0	22.0	40.0
49	32.87107580658313	35.0	32.0	38.0	21.0	40.0
50	32.71427794841129	35.0	32.0	38.0	20.0	40.0
51	32.51766170168429	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	14.0
15	40.0
16	138.0
17	343.0
18	804.0
19	1553.0
20	2510.0
21	3951.0
22	5841.0
23	8290.0
24	11241.0
25	15328.0
26	20104.0
27	23235.0
28	24912.0
29	26851.0
30	31406.0
31	37803.0
32	46331.0
33	59424.0
34	101209.0
35	137124.0
36	56898.0
37	64999.0
38	86524.0
39	116067.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.183351000697634	24.15128699953793	27.937992081396718	16.727369918367714
2	31.744856078932347	25.50544517227945	29.29803937557193	13.451659373216277
3	27.937992081396718	25.120500484719994	33.610801554728056	13.330705879155227
4	25.80171328132786	27.969249725929345	31.314610457267626	14.914426535475162
5	22.168125356744856	31.617560454096566	31.61303036068604	14.601283828472544
6	20.947038677937538	40.11318438386199	29.110267003705616	9.82950993449485
7	84.70629139372853	4.696461091027697	8.726998450708054	1.8702490645357106
8	86.55185144917688	3.743782446794053	7.993576327543874	1.7107897764851911
9	82.46276263216548	4.902806845877162	10.115245576363785	2.519184945593578
10	56.52820436157393	18.860024643708154	14.625066818877805	9.986704175840105
11	52.693706794234096	17.95264693357977	18.837034419649733	10.5166118525364
12	46.85747420111802	20.305124441665985	21.992697489422234	10.844703867793754
13	21.064368097270165	43.54767470305237	22.89067525572377	12.497281943953684
14	14.006256058999936	46.0986835548549	26.80999882217571	13.08506156396945
15	12.717331231369991	21.941733938553813	53.50334773903038	11.837587091045817
16	13.710893968633634	15.702889293577233	52.817378344341456	17.768838393447673
17	14.231741458508873	16.858063113261395	30.089446694390837	38.82074873383889
18	20.712719596278077	22.017159993839073	37.358774337926846	19.911346071956004
19	31.442698848450256	21.242061011298052	27.401855526260952	19.91338461399074
20	33.609216022034374	20.761644605111755	25.93104744819838	19.698091924655486
21	22.205045618040646	28.071743089342505	28.96235945385194	20.760851838764914
22	23.25364899024218	25.182562764444206	24.680741666893173	26.88304657842045
23	19.133755538039193	31.23340853288395	25.149946091888413	24.48288983718844
24	18.76727098112763	22.71207632301378	42.70417131001241	15.816481385846176
25	16.32634339920089	23.467242894548484	40.40220434345356	19.804209362797064
26	14.760629864187798	35.61491487954482	29.75082221195401	19.873633044313372
27	17.22568019352559	36.93373832368423	29.594760493961385	16.24582098882879
28	13.969449050039412	29.911527275692425	41.08896650448932	15.030057169778841
29	14.07567974051625	24.053663486541094	40.36641660551041	21.504240167432254
30	17.14130720375454	31.73409710708235	33.05631812127966	18.06827756788345
31	29.96067878919663	26.393116976072044	26.053020213276795	17.593184021454523
32	32.17963179400759	25.166707437507362	27.41657832984516	15.237082438639884
33	30.276199795239776	26.753146149873608	25.160705063738416	17.809948991148197
34	19.97329509934495	26.849637139517817	28.159287144500922	25.017780616636315
35	19.860042764081793	24.97519773857737	32.3889221095739	22.775837387766938
36	32.09695758926549	24.176542270301614	26.17453996901416	17.551960171418735
37	20.805020249517543	33.735605628188054	29.66894077355875	15.79043334873565
38	20.510677430168613	34.928492475514844	22.69452221104799	21.866307883268554
39	20.744656754822284	33.337183912732286	27.195509771411487	18.72264956103395
40	25.022197457711577	25.5716977884084	25.08799706449947	24.318107689380554
41	16.001875458671957	24.579720583838437	27.626774664093574	31.791629293396028
42	21.724062950178034	24.79206871245685	24.396138548376868	29.08772978898825
43	21.42734183178857	27.13741132342149	25.51903545251103	25.91621139227891
44	18.832730830909732	31.745648845279188	28.375259347847752	21.046360975963324
45	15.581482790175134	39.91374702146358	22.643218903173786	21.8615512851875
46	20.570361410852293	35.35545377945693	25.63387332046787	18.440311489222907
47	21.97854094751434	27.319521078524637	27.483850216991474	23.21808775696955
48	23.987524122747413	23.892392161126363	31.807371367997604	20.312712348128617
49	21.428134598135415	23.690463247352163	33.51204551837859	21.369356636133837
50	19.432741703133917	35.86780734418743	26.242604622507315	18.456846330171327
51	18.29297020104555	35.54707673072219	23.47392478232901	22.686028285903255
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	635.0
1	994.0
2	1353.0
3	4996.5
4	8640.0
5	6655.0
6	4670.0
7	4952.0
8	5234.0
9	5636.0
10	6038.0
11	6089.5
12	6141.0
13	6096.5
14	6052.0
15	5708.0
16	5364.0
17	4833.0
18	4302.0
19	4194.0
20	4086.0
21	3835.0
22	3584.0
23	3466.0
24	3348.0
25	3878.0
26	5850.5
27	7293.0
28	8479.0
29	9665.0
30	10429.0
31	11193.0
32	12664.0
33	14135.0
34	16547.0
35	18959.0
36	20736.0
37	22513.0
38	27780.0
39	33047.0
40	49391.5
41	65736.0
42	81887.5
43	98039.0
44	98226.0
45	98413.0
46	91164.5
47	83916.0
48	76830.0
49	69744.0
50	65264.5
51	60785.0
52	56756.5
53	52728.0
54	46561.0
55	40394.0
56	37549.0
57	34704.0
58	32130.0
59	29556.0
60	27814.5
61	26073.0
62	21654.0
63	17235.0
64	13841.0
65	10447.0
66	8255.5
67	6064.0
68	4823.0
69	3582.0
70	2770.5
71	1959.0
72	1617.5
73	1276.0
74	1044.0
75	582.0
76	352.0
77	297.0
78	242.0
79	214.0
80	186.0
81	115.5
82	45.0
83	38.0
84	31.0
85	17.5
86	4.0
87	2.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	882984.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.115414608120577
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.23769598442898	15.72007655158933
2	5.478343177325736	2.0944160240030016
3	1.707395693044968	0.9791272971802184
4	0.9314869138373091	0.7122303424015541
5	0.5915503313400957	0.5653864922568517
6	0.45432341769946144	0.5210748297302131
7	0.37227448888976733	0.4981326842207856
8	0.31534686809762463	0.48223889032467254
9	0.2787260927158317	0.47951683419281227
>10	4.422164804381204	20.846132110142364
>50	2.1836971491454116	30.301813214079598
>100	1.0240337322680815	25.613930278759856
>500	0.0011845387302117773	0.17763393449215537
>1k	0.0017768080953176658	1.0082905166266
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4688	0.5309269477136619	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3140	0.35561233272630083	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1078	0.12208601741367908	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	885	0.10022831670789052	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.010645719514736394	0.0
2	0.0	0.0	0.0	0.04269613039420873	0.0
3	0.0	0.0	0.0	0.057079176972629174	0.0
4	0.0	0.0	0.0	0.0906018682105225	0.0
5	0.0	0.0	0.0	0.14632201715999385	0.0
6	0.0	0.0	0.0	0.2116686146068332	0.0
7	0.0	0.0	0.0	0.2498346515905158	0.0
8	0.0	0.0	0.0	0.36546528589419514	0.0
9	0.0	0.0	0.0	0.4083879209589302	0.0
10	0.0	0.0	0.0	0.4810959201978745	0.0
11	0.0	0.0	0.0	0.5528979007547136	0.0
12	0.0	0.0	0.0	0.6132613954499742	0.0
13	0.0	0.0	0.0	0.6423672456126045	0.0
14	0.0	0.0	0.0	0.6518804417747094	0.0
15	0.0	0.0	0.0	0.666376740688393	0.0
16	0.0	0.0	0.0	0.7021644786315494	0.0
17	0.0	0.0	0.0	0.7398775062741794	0.0
18	0.0	0.0	0.0	0.8107734681489133	0.0
19	0.0	0.0	0.0	0.835009467895228	0.0
20	0.0	0.0	0.0	0.863888813387332	0.0
21	0.0	0.0	0.0	0.9028476167178567	0.0
22	0.0	0.0	0.0	0.9446377284299602	0.0
23	0.0	0.0	0.0	0.9942422512752213	0.0
24	0.0	0.0	0.0	1.0322950359236407	0.0
25	0.0	0.0	0.0	1.0609478767452185	0.0
26	0.0	0.0	0.0	1.090959745589954	0.0
27	0.0	0.0	0.0	1.1201788480878476	0.0
28	0.0	0.0	0.0	1.1510967356146884	0.0
29	0.0	0.0	0.0	1.183260398829424	0.0
30	0.0	0.0	0.0	1.2338841926920534	0.0
31	0.0	0.0	0.0	1.2819031828436303	0.0
32	0.0	0.0	0.0	1.3230137805441549	0.0
33	0.0	0.0	0.0	1.364577387585732	0.0
34	0.0	0.0	0.0	1.4065940039683618	0.0
35	0.0	0.0	0.0	1.471827349079938	0.0
36	0.0	0.0	0.0	1.521998133601515	0.0
37	0.0	0.0	0.0	1.5752267311751968	0.0
38	0.0	0.0	0.0	1.6252842633615106	0.0
39	1.1325233526315313E-4	0.0	0.0	1.6734165058483506	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGAGAC	30	2.1649284E-6	45.000004	21
TAGAGTC	25	3.890068E-5	45.000004	16
AACCGTG	25	3.890068E-5	45.000004	20
TCGTTAG	30	2.1649284E-6	45.000004	1
GTACCGT	25	3.890068E-5	45.000004	15
GTACAAC	30	2.1649284E-6	45.000004	41
ATAATCG	25	3.890068E-5	45.000004	45
CACGCTC	30	2.1649284E-6	45.000004	13
TACGGGT	85	0.0	45.000004	4
TGCATCG	25	3.890068E-5	45.000004	24
CTACGTG	30	2.1649284E-6	45.000004	1
CGCTATA	30	2.1649284E-6	45.000004	39
CTACGAA	60	0.0	45.000004	11
TACCTAG	30	2.1649284E-6	45.000004	1
CGAATGT	25	3.890068E-5	45.000004	17
ACCGTTC	25	3.890068E-5	45.000004	17
GCTACGA	60	0.0	45.000004	10
ATACGTA	25	3.890068E-5	45.000004	44
CCGTTTA	25	3.890068E-5	45.000004	23
TGCACGG	60	0.0	45.000004	2
>>END_MODULE