##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549035_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1800160
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.90288252155364	31.0	31.0	33.0	28.0	34.0
2	31.193308372589104	31.0	31.0	34.0	28.0	34.0
3	31.273938983201493	31.0	31.0	34.0	28.0	34.0
4	35.124359501377654	35.0	35.0	37.0	32.0	37.0
5	34.98183161496756	35.0	35.0	37.0	32.0	37.0
6	35.03415918585014	37.0	35.0	37.0	32.0	37.0
7	35.53772164696471	37.0	35.0	37.0	35.0	37.0
8	35.45733768109501	37.0	35.0	37.0	35.0	37.0
9	37.12343958314816	39.0	37.0	39.0	34.0	39.0
10	36.525247755755046	38.0	35.0	39.0	32.0	39.0
11	36.24066749622256	38.0	35.0	39.0	31.0	39.0
12	35.676105457292685	37.0	35.0	39.0	30.0	39.0
13	35.332935405741715	37.0	35.0	39.0	30.0	39.0
14	36.367397342458446	38.0	35.0	40.0	30.0	41.0
15	36.6294301617634	38.0	35.0	40.0	31.0	41.0
16	36.66198393476135	38.0	35.0	40.0	31.0	41.0
17	36.55494511598969	38.0	35.0	40.0	31.0	41.0
18	36.467018487245575	38.0	35.0	40.0	31.0	41.0
19	36.37687538885432	38.0	35.0	40.0	30.0	41.0
20	36.2422545773709	38.0	34.0	40.0	30.0	41.0
21	36.09219069416052	38.0	34.0	40.0	30.0	41.0
22	35.96660519064972	37.0	34.0	40.0	30.0	41.0
23	35.87160974580037	37.0	34.0	40.0	30.0	41.0
24	35.76024631143898	37.0	34.0	40.0	30.0	41.0
25	35.602624766687406	37.0	34.0	40.0	29.0	41.0
26	35.44479046306995	37.0	34.0	40.0	29.0	41.0
27	35.36051850946583	37.0	34.0	40.0	29.0	41.0
28	35.37792640654164	37.0	34.0	40.0	29.0	41.0
29	35.38812605546174	37.0	34.0	40.0	29.0	41.0
30	35.26236390098658	37.0	34.0	40.0	28.0	41.0
31	35.13875211092348	37.0	34.0	40.0	27.0	41.0
32	34.91672406897165	37.0	34.0	40.0	26.0	41.0
33	34.6662007821527	37.0	33.0	40.0	25.0	41.0
34	34.46650019998222	37.0	33.0	40.0	24.0	41.0
35	34.27076370989246	36.0	33.0	40.0	23.0	41.0
36	34.084933561461206	36.0	33.0	40.0	23.0	41.0
37	34.031792729535155	36.0	33.0	40.0	23.0	41.0
38	33.98765165318638	36.0	33.0	40.0	23.0	41.0
39	33.88665951915385	36.0	33.0	40.0	23.0	41.0
40	33.721192005155096	36.0	33.0	40.0	22.0	41.0
41	33.622787418896095	36.0	32.0	40.0	22.0	41.0
42	33.58160274642254	36.0	32.0	40.0	22.0	41.0
43	33.50298862323349	35.0	32.0	40.0	21.0	41.0
44	33.408028730779485	35.0	32.0	40.0	21.0	41.0
45	33.33948482357124	35.0	32.0	40.0	21.0	41.0
46	33.25336803395254	35.0	32.0	39.0	21.0	41.0
47	33.179408497022486	35.0	32.0	39.0	21.0	41.0
48	33.102064260954585	35.0	32.0	39.0	20.0	41.0
49	33.079809572482446	35.0	32.0	39.0	20.0	41.0
50	33.01316160785708	35.0	32.0	39.0	20.0	41.0
51	32.838863767665096	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	3.0
13	6.0
14	24.0
15	90.0
16	228.0
17	701.0
18	1529.0
19	2917.0
20	4692.0
21	7455.0
22	11014.0
23	15396.0
24	21202.0
25	28916.0
26	37756.0
27	43640.0
28	47540.0
29	52955.0
30	60879.0
31	72139.0
32	86927.0
33	108772.0
34	163248.0
35	204070.0
36	143614.0
37	171408.0
38	225526.0
39	287373.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.1956381654964	23.37208914763132	27.14392054039641	17.28835214647587
2	33.3042618433917	25.911307883743667	27.687316682961516	13.09711358990312
3	28.839992000711046	25.172873522353566	32.48144609368056	13.505688383254821
4	27.339903119722692	27.09742467336237	30.954859568038394	14.607812638876544
5	23.15866367434006	31.529530708381476	30.657441560750154	14.654364056528307
6	21.285607945960358	40.126155452848636	28.26676517642876	10.321471424762244
7	83.41536307883743	5.252310905697271	9.40694160519065	1.9253844102746422
8	83.86226779841792	4.607479335170208	9.450659941338548	2.0795929250733267
9	78.3237045595947	6.595247089147631	11.275608834770242	3.805439516487423
10	44.300173317927296	25.237534441382987	17.645375966580747	12.81691627410897
11	38.17338458803662	23.581070571504757	23.752777530886142	14.492767309572482
12	34.92628433028175	21.83428139720914	28.19021642520665	15.049217847302462
13	22.500611056794952	32.190860812372236	28.232490445293752	17.076037685539063
14	17.233357034930226	35.13115500844369	29.748744556039462	17.886743400586614
15	17.661818949426717	23.658341480757265	42.4810572393565	16.198782330459515
16	18.90070882588214	20.212258910319083	41.71068127277575	19.17635099102302
17	18.75466625188872	20.698437916629633	31.491756288329924	29.05513954315172
18	21.8847213581015	23.77710870144876	33.293318371700295	21.044851568749444
19	29.041529641809618	23.760276864278733	27.72959070304862	19.468602790863034
20	30.475124433383698	22.395009332503776	27.76214336503422	19.367722869078303
21	24.678639676473203	25.597557994844905	28.97903519687139	20.74476713181051
22	23.569849346724737	25.41957381566083	27.29362945515954	23.716947382454894
23	22.502944182739313	28.07456003910763	27.361790063105502	22.06070571504755
24	21.28038618789441	23.609512487778865	35.92758421473647	19.182517109590258
25	21.01629855123989	25.074826682072704	33.36392320682606	20.544951559861346
26	19.64430939472047	30.494178295262643	28.790163096613636	21.071349213403252
27	20.809372500222203	30.446571415874146	29.444049417829525	19.300006666074125
28	18.840103101946493	28.243156163896543	34.59981334992445	18.31692738423251
29	19.600313305483958	25.764376499866675	33.84510265754155	20.790207537107815
30	20.177039818682786	28.074893342813972	31.41504088525464	20.3330259532486
31	25.7078259710248	26.891776286552304	27.21335881255	20.187038929872898
32	27.538607679317394	26.580081770509288	28.176828726335433	17.70448182383788
33	26.28794107190472	27.510387965514177	26.4292618433917	19.772409119189405
34	21.458814772020265	28.029286285663495	28.760554617367344	21.751344324948892
35	22.5592725091103	25.809316949604476	29.787018931650522	21.8443916096347
36	26.176895387076705	28.658563683228156	26.160063549906674	19.00447737978846
37	22.356235001333218	32.01570971469202	27.738812105590615	17.88924317838414
38	22.367956181672742	30.39646475868812	25.77904186294552	21.456537196693628
39	22.65065327526442	30.383854768465024	26.585081326104348	20.380410630166207
40	23.93909430272865	27.270853701893166	27.580770598169053	21.209281397209136
41	19.118745000444402	27.08842547329126	28.807439338725448	24.985390187538886
42	22.114200959914672	28.081614967558437	25.48734556928273	24.316838503244156
43	23.007565994133856	27.58149275619945	26.74078970758155	22.670151542085147
44	21.179172962403342	29.768298373477915	27.67315127544218	21.379377388676563
45	19.891231890498624	32.676873166829616	25.278919651586524	22.152975291085237
46	20.466569638254377	32.37662207803751	26.409652475335527	20.74715580837259
47	22.905741711847835	28.691394098302375	27.581881610523507	20.820982579326284
48	23.353035285752377	25.821760287974406	29.422106923828995	21.403097502444226
49	21.1431206115012	27.193249488934317	30.514398720113768	21.149231179450716
50	20.148375699937784	31.315827482001602	27.351513198826773	21.184283619233845
51	19.76807617100702	30.98030175095547	26.467536219002756	22.784085859034754
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1384.0
1	2751.0
2	4118.0
3	14669.5
4	25221.0
5	18558.5
6	11896.0
7	11912.5
8	11929.0
9	12267.5
10	12606.0
11	12983.0
12	13360.0
13	12718.0
14	12076.0
15	11467.5
16	10859.0
17	10533.0
18	10207.0
19	9773.5
20	9340.0
21	9637.5
22	9935.0
23	10531.0
24	11127.0
25	12390.0
26	15125.5
27	16598.0
28	21800.5
29	27003.0
30	29724.0
31	32445.0
32	36594.0
33	40743.0
34	45745.0
35	50747.0
36	50041.0
37	49335.0
38	57807.0
39	66279.0
40	84349.5
41	102420.0
42	117215.5
43	132011.0
44	137107.0
45	142203.0
46	137858.0
47	133513.0
48	126501.0
49	119489.0
50	116672.0
51	113855.0
52	110368.0
53	106881.0
54	101873.5
55	96866.0
56	95883.0
57	94900.0
58	92657.5
59	90415.0
60	84138.0
61	77861.0
62	66437.0
63	55013.0
64	45291.5
65	35570.0
66	28216.0
67	20862.0
68	17480.5
69	14099.0
70	11603.5
71	9108.0
72	7721.0
73	6334.0
74	5102.5
75	2911.0
76	1951.0
77	1508.5
78	1066.0
79	826.5
80	587.0
81	419.5
82	252.0
83	177.5
84	103.0
85	68.5
86	34.0
87	26.0
88	18.0
89	14.5
90	11.0
91	7.0
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1800160.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.183024080469437
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.31182201446654	16.007524135838842
2	5.653613359075262	2.282140291357594
3	2.1855279390644142	1.3233168906802746
4	1.3045428423048806	1.053184784009738
5	0.9370181884218467	0.9455930330377992
6	0.6988890575392639	0.8463416806734932
7	0.5577023600090871	0.7879284113258629
8	0.47967003298929206	0.774495346120196
9	0.4340684651088521	0.7884732855477932
>10	5.76226824273981	27.518526184792552
>50	2.0859940141407627	30.06259215869646
>100	0.582524648400305	15.482220930912563
>500	0.004147066786740503	0.5633033475592635
>1k	0.0019352978338122349	0.8115027819271292
>5k	0.0	0.0
>10k+	2.7647111911603353E-4	0.7528567375205134
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	13492	0.7494889343169495	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3348	0.18598346813616567	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2844	0.15798595680383964	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2473	0.13737667762865524	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.555061772286908E-5	0.0	0.0	0.011776730957248245	0.0
2	5.555061772286908E-5	0.0	0.0	0.04188516576304328	0.0
3	5.555061772286908E-5	0.0	0.0	0.058717002933072616	0.0
4	5.555061772286908E-5	0.0	0.0	0.08310372411341214	0.0
5	5.555061772286908E-5	0.0	0.0	0.13309928006399432	0.0
6	5.555061772286908E-5	0.0	0.0	0.21475868811661186	0.0
7	5.555061772286908E-5	0.0	0.0	0.26436538974313395	0.0
8	5.555061772286908E-5	0.0	0.0	0.38813216602968625	0.0
9	5.555061772286908E-5	0.0	0.0	0.45929250733268157	0.0
10	1.1110123544573816E-4	0.0	0.0	0.5712825526619856	0.0
11	1.1110123544573816E-4	0.0	0.0	0.6684961336770064	0.0
12	1.1110123544573816E-4	0.0	0.0	0.7555439516487423	0.0
13	1.1110123544573816E-4	0.0	0.0	0.7971513643231712	0.0
14	1.1110123544573816E-4	0.0	0.0	0.815094213847658	0.0
15	1.1110123544573816E-4	0.0	0.0	0.8377033152608657	0.0
16	1.1110123544573816E-4	0.0	0.0	0.8803661896720292	0.0
17	1.6665185316860724E-4	0.0	0.0	0.9283063727668651	0.0
18	1.6665185316860724E-4	0.0	0.0	0.9983557017154031	0.0
19	1.6665185316860724E-4	0.0	0.0	1.0312972180250644	0.0
20	1.6665185316860724E-4	0.0	0.0	1.0656274997777975	0.0
21	1.6665185316860724E-4	0.0	0.0	1.106957159363612	0.0
22	1.6665185316860724E-4	0.0	0.0	1.1548417918407252	0.0
23	1.6665185316860724E-4	0.0	0.0	1.2051706514976446	0.0
24	1.6665185316860724E-4	0.0	0.0	1.2486112345569282	0.0
25	1.6665185316860724E-4	0.0	0.0	1.287274464492045	0.0
26	1.6665185316860724E-4	0.0	0.0	1.3236045684828015	0.0
27	1.6665185316860724E-4	0.0	0.0	1.3643231712736645	0.0
28	2.2220247089147632E-4	0.0	0.0	1.4050417740645276	0.0
29	2.2220247089147632E-4	0.0	0.0	1.4517598435694605	0.0
30	2.2220247089147632E-4	0.0	0.0	1.5150875477735313	0.0
31	2.2220247089147632E-4	0.0	0.0	1.5636387876633189	0.0
32	2.2220247089147632E-4	0.0	0.0	1.6178006399431162	0.0
33	2.2220247089147632E-4	0.0	0.0	1.6701293218380588	0.0
34	2.2220247089147632E-4	0.0	0.0	1.7202359790240866	0.0
35	2.2220247089147632E-4	0.0	0.0	1.785952359790241	0.0
36	2.2220247089147632E-4	0.0	0.0	1.8435027997511333	0.0
37	2.777530886143454E-4	0.0	0.0	1.9032752644209403	0.0
38	2.777530886143454E-4	0.0	0.0	1.9646586970047106	0.0
39	2.777530886143454E-4	0.0	0.0	2.0293196160341305	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATTACG	20	7.0343516E-4	45.000004	37
TATTACG	45	3.8562575E-10	45.0	1
TACGTAG	45	3.8562575E-10	45.0	1
ATACCGG	30	2.1661908E-6	44.999996	2
ATTACGG	140	0.0	43.392857	2
CGTTTTT	10080	0.0	40.736603	1
TCGATTG	45	1.929402E-8	40.0	1
CGCATCG	85	0.0	39.705883	21
CACGACG	155	0.0	39.193546	26
ACTCACG	35	6.2502622E-6	38.571426	42
TATAGCG	100	0.0	38.25	1
TATACTA	1110	0.0	37.905403	44
ATAGGGC	930	0.0	37.741936	4
CTATGCG	60	1.5643309E-10	37.499996	1
AACGCGT	30	1.1401788E-4	37.499996	32
TGGGCGA	1105	0.0	37.46606	6
GTTGATC	1070	0.0	37.219627	16
ATAACGC	115	0.0	37.173912	11
TACGGGA	480	0.0	37.031246	4
CGAGGGA	810	0.0	36.944447	4
>>END_MODULE