##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549032_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1197847
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.85184585343537	31.0	31.0	33.0	28.0	34.0
2	31.139441848583335	31.0	31.0	34.0	28.0	34.0
3	31.243372484131946	31.0	31.0	34.0	28.0	34.0
4	35.08246295228022	35.0	35.0	37.0	32.0	37.0
5	34.94738810549261	35.0	35.0	37.0	32.0	37.0
6	34.99621738001598	37.0	35.0	37.0	32.0	37.0
7	35.49186665742787	36.0	35.0	37.0	35.0	37.0
8	35.40675812520297	37.0	35.0	37.0	35.0	37.0
9	37.10120491181261	39.0	37.0	39.0	34.0	39.0
10	36.50573570748184	38.0	35.0	39.0	32.0	39.0
11	36.1551901035775	38.0	35.0	39.0	31.0	39.0
12	35.32841005570828	37.0	35.0	39.0	30.0	39.0
13	34.850420796645984	37.0	33.0	39.0	27.0	39.0
14	35.79464071788801	38.0	34.0	40.0	27.0	41.0
15	36.138268910804136	38.0	34.0	40.0	30.0	41.0
16	36.24278392816445	38.0	34.0	40.0	31.0	41.0
17	36.138529378125924	38.0	34.0	40.0	30.0	41.0
18	36.083706850707976	37.0	34.0	40.0	30.0	41.0
19	36.002699843970056	37.0	34.0	40.0	30.0	41.0
20	35.85399220434663	37.0	34.0	40.0	30.0	41.0
21	35.69537177953445	36.0	34.0	40.0	30.0	41.0
22	35.57568871483587	36.0	34.0	40.0	29.0	41.0
23	35.50777686966699	36.0	34.0	40.0	29.0	41.0
24	35.41342675650563	36.0	34.0	40.0	29.0	41.0
25	35.24659159308326	36.0	34.0	40.0	29.0	41.0
26	35.06570455158297	35.0	34.0	40.0	28.0	41.0
27	34.976501172520365	36.0	34.0	40.0	27.0	41.0
28	35.02072468353638	36.0	34.0	40.0	27.0	41.0
29	35.06206969671419	36.0	34.0	40.0	28.0	41.0
30	34.93282030175807	36.0	34.0	40.0	27.0	41.0
31	34.750645950609716	36.0	33.0	40.0	27.0	41.0
32	34.51302712282954	35.0	33.0	40.0	25.0	41.0
33	34.270714874270254	35.0	33.0	40.0	24.0	41.0
34	34.08007366550152	35.0	33.0	40.0	23.0	41.0
35	33.90154836135166	35.0	33.0	40.0	23.0	41.0
36	33.700642068644825	35.0	33.0	40.0	22.0	41.0
37	33.66851526113101	35.0	33.0	40.0	23.0	41.0
38	33.66071710326945	35.0	33.0	40.0	23.0	41.0
39	33.57921003266694	35.0	32.0	40.0	23.0	41.0
40	33.415588134377764	35.0	32.0	40.0	22.0	41.0
41	33.38159213989767	35.0	32.0	40.0	22.0	41.0
42	33.316717410487314	35.0	32.0	39.0	22.0	41.0
43	33.19468346124338	35.0	32.0	39.0	21.0	41.0
44	33.09874466438535	35.0	32.0	39.0	21.0	41.0
45	33.04167059733004	35.0	32.0	39.0	21.0	41.0
46	32.96747414319191	35.0	32.0	39.0	21.0	41.0
47	32.88489681904283	35.0	31.0	39.0	20.0	41.0
48	32.876989298299364	35.0	31.0	39.0	20.0	41.0
49	32.906589906724314	35.0	31.0	39.0	20.0	41.0
50	32.84012983294193	35.0	32.0	39.0	20.0	40.0
51	32.656060415061354	35.0	31.0	38.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	7.0
12	1.0
13	6.0
14	18.0
15	66.0
16	197.0
17	524.0
18	1127.0
19	2174.0
20	3544.0
21	5604.0
22	8065.0
23	11249.0
24	14877.0
25	20604.0
26	26144.0
27	30492.0
28	33242.0
29	36942.0
30	42729.0
31	50404.0
32	61756.0
33	77359.0
34	122079.0
35	154158.0
36	88206.0
37	103464.0
38	134760.0
39	167989.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.41273718596782	23.31458024271881	27.532982092036796	16.739700479276568
2	32.35062574769565	25.634909967633597	29.157897461027993	12.856566823642751
3	28.930739902508417	25.05102905462885	32.65124844825758	13.366982594605153
4	26.275475916373292	27.59784847313555	31.27786770764547	14.84880790284569
5	22.724855511597056	32.287512512032	30.845675616334972	14.141956360035964
6	21.707947676122245	40.225588075939584	28.34886258428664	9.717601663651534
7	83.30237501116586	5.38115468837005	9.328403377059006	1.9880669234050758
8	84.47013683717537	4.43094986254505	8.927433971116512	2.1714793291630734
9	79.96655666374754	5.929638760208941	10.750872189853963	3.3529323861895546
10	50.15164708013627	21.832003586434663	16.058311286833792	11.958038046595266
11	44.138191271506294	20.261853141511395	22.47607582604456	13.123879760937749
12	40.23368593818743	20.731195219422847	25.65912007126119	13.375998771128533
13	22.949258127290044	35.80724416390407	25.295217168803696	15.948280540002187
14	17.158117856454123	36.56335074512855	30.041566243435096	16.23696515498223
15	16.641023436215143	21.992708584652295	46.132018529912415	15.234249449220142
16	18.732025041595463	18.337483835581672	43.553392044226015	19.37709907859685
17	18.029848553279344	19.39805334070211	30.700999376381123	31.871098729637424
18	22.20801154070595	22.695803387243945	34.55040585316823	20.545779218881876
19	30.099420042793447	22.78855312907241	26.8374007698813	20.27462605825285
20	31.308923426781547	22.413380005960697	26.826881897270688	19.450814669987068
21	24.11802175069103	27.121326847251776	28.25427621390712	20.506375188150074
22	24.03395425292212	24.393849965813665	26.175379660340592	25.396816120923628
23	21.881258624849416	28.503640281271313	27.06881596731469	22.546285126564577
24	21.066546896222974	22.27471455035576	38.82257082916266	17.83616772425861
25	19.68565267517471	23.872330940428952	35.666074214820426	20.775942169575913
26	17.974248798051836	31.569557714799974	28.962880902151944	21.493312584996247
27	18.625667551865973	31.62741151415832	29.357338625049778	20.38958230892593
28	16.759652943990343	27.367602039325554	37.18287894864703	18.68986606803707
29	18.014821592407042	24.546039686203663	36.47101841887987	20.96812030250942
30	19.812547011429675	29.109644220004725	31.82534998209287	19.25245878647273
31	28.56399857410838	25.716639938155705	26.9045211951109	18.814840292625018
32	31.24422401191471	24.14148050627501	27.293051616775767	17.321243865034518
33	28.004077315383352	26.72428114775927	25.766228909034293	19.505412627823084
34	20.466303292490608	26.805426736469684	29.447500390283565	23.28076958075614
35	21.609354116176775	25.354072765553532	31.33179780055383	21.704775317715868
36	28.063934709524673	28.035383483867303	25.800456986576748	18.100224820031272
37	22.0297750881373	33.188712748790124	27.355330021279844	17.426182141792733
38	21.202039993421533	32.900445549389865	24.69864682217345	21.198867635015155
39	22.180879528019855	32.24101241644384	26.01876533480486	19.559342720731447
40	24.71459209732128	26.09481845344188	25.806718220273538	23.383871228963297
41	17.956967793048694	25.997727589583647	27.061469453110455	28.983835164257204
42	21.900626707751492	25.676067143800502	24.253097432309804	28.170208716138205
43	21.78349989606352	26.52759492656408	25.894709424492447	25.79419575287996
44	20.023341879221636	29.32185830076796	28.18707230556156	22.46772751444884
45	19.038408077158437	34.679971649133826	23.97459775747654	22.307022516231203
46	20.997255909978488	33.01030932998955	25.64918558046228	20.34324917956968
47	21.401648123675226	27.48464536789757	27.608283862630202	23.505422645797
48	22.723519781741743	24.026774704949798	32.050587428945434	21.19911808436303
49	20.996588045050828	25.597342565452852	32.1248039190314	21.281265470464923
50	19.397886374470193	32.24076196709596	27.500423676813483	20.860927981620357
51	18.410865494508062	32.28434015362563	25.94388097979124	23.360913372075064
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	970.0
1	1870.0
2	2770.0
3	8982.0
4	15194.0
5	11130.0
6	7066.0
7	7030.5
8	6995.0
9	7313.0
10	7631.0
11	7754.0
12	7877.0
13	7935.5
14	7994.0
15	7711.0
16	7428.0
17	6825.5
18	6223.0
19	6249.5
20	6276.0
21	6339.0
22	6402.0
23	6065.0
24	5728.0
25	6118.0
26	7212.0
27	7916.0
28	9933.0
29	11950.0
30	15398.0
31	18846.0
32	21890.0
33	24934.0
34	27572.0
35	30210.0
36	29957.5
37	29705.0
38	34699.0
39	39693.0
40	57258.5
41	74824.0
42	88437.0
43	102050.0
44	104144.0
45	106238.0
46	99518.5
47	92799.0
48	87414.5
49	82030.0
50	81537.5
51	81045.0
52	77204.5
53	73364.0
54	69205.0
55	65046.0
56	63059.5
57	61073.0
58	59968.0
59	58863.0
60	54118.0
61	49373.0
62	42872.0
63	36371.0
64	29763.5
65	23156.0
66	18052.5
67	12949.0
68	10399.5
69	7850.0
70	6415.0
71	4980.0
72	4156.5
73	3333.0
74	2607.5
75	1493.0
76	1104.0
77	859.0
78	614.0
79	471.0
80	328.0
81	245.0
82	162.0
83	109.0
84	56.0
85	39.5
86	23.0
87	16.0
88	9.0
89	5.0
90	1.0
91	2.5
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1197847.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.355067140441246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.99375492438024	16.07923185161321
2	5.701281198869328	2.320999231790411
3	2.21599179433312	1.3531998526895257
4	1.327534230185143	1.0808819354661026
5	0.9009226330904322	0.9169170342449429
6	0.6518011981450111	0.796047429027705
7	0.5989373642486415	0.8533987183540007
8	0.49882953385960005	0.8122966922677735
9	0.44593891818604425	0.8169404958191401
>10	6.028145697127431	28.67151894637875
>50	2.051672032684684	29.74170758822095
>100	0.5810665110081181	15.195127766103194
>500	0.002474378329346138	0.29841936187130896
>1k	0.001237189164673069	0.4482586195197721
>5k	4.123963882243564E-4	0.6150544766332153
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7327	0.6116807906185013	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3110	0.25963249062693317	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0070960648563631245	0.0
2	0.0	0.0	0.0	0.02404313739567741	0.0
3	0.0	0.0	0.0	0.03548032428181563	0.0
4	0.0	0.0	0.0	0.05927301232962139	0.0
5	0.0	0.0	0.0	0.10301816509120112	0.0
6	0.0	0.0	0.0	0.17331094872717467	0.0
7	8.348311595721323E-5	0.0	0.0	0.2149690235898241	0.0
8	8.348311595721323E-5	0.0	0.0	0.34495223513520507	0.0
9	8.348311595721323E-5	0.0	0.0	0.3955430034052763	0.0
10	8.348311595721323E-5	0.0	0.0	0.4630808442146618	0.0
11	8.348311595721323E-5	0.0	0.0	0.5195989137176952	0.0
12	8.348311595721323E-5	0.0	0.0	0.57787012865583	0.0
13	8.348311595721323E-5	0.0	0.0	0.6049186582259671	0.0
14	8.348311595721323E-5	0.0	0.0	0.6172741593876346	0.0
15	8.348311595721323E-5	0.0	0.0	0.6316332553322753	0.0
16	8.348311595721323E-5	0.0	0.0	0.6604349303375139	0.0
17	8.348311595721323E-5	0.0	0.0	0.6920750312852977	0.0
18	8.348311595721323E-5	0.0	0.0	0.7409126541202674	0.0
19	8.348311595721323E-5	0.0	0.0	0.7613660175297847	0.0
20	8.348311595721323E-5	0.0	0.0	0.7879971315201357	0.0
21	8.348311595721323E-5	0.0	0.0	0.8196372324679195	0.0
22	8.348311595721323E-5	0.0	0.0	0.8547836242859063	0.0
23	8.348311595721323E-5	0.0	0.0	0.8939372056698393	0.0
24	8.348311595721323E-5	0.0	0.0	0.9290835974878261	0.0
25	8.348311595721323E-5	0.0	0.0	0.9598053841600805	0.0
26	8.348311595721323E-5	0.0	0.0	0.9870208799621321	0.0
27	8.348311595721323E-5	0.0	0.0	1.011314466705681	0.0
28	8.348311595721323E-5	0.0	0.0	1.0430380507694221	0.0
29	8.348311595721323E-5	0.0	0.0	1.07785051012358	0.0
30	8.348311595721323E-5	0.0	0.0	1.1234322914362185	0.0
31	8.348311595721323E-5	0.0	0.0	1.1645894676031245	0.0
32	8.348311595721323E-5	0.0	0.0	1.1990679944934537	0.0
33	8.348311595721323E-5	0.0	0.0	1.2408930355880174	0.0
34	8.348311595721323E-5	0.0	0.0	1.2845547052336401	0.0
35	8.348311595721323E-5	0.0	0.0	1.3389856968377432	0.0
36	8.348311595721323E-5	0.0	0.0	1.3873224209769695	0.0
37	8.348311595721323E-5	0.0	0.0	1.4379131892470407	0.0
38	8.348311595721323E-5	0.0	0.0	1.4945147418660314	0.0
39	8.348311595721323E-5	0.0	0.0	1.5523685412243802	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGGA	30	2.1655778E-6	45.000004	37
CTATACG	30	2.1655778E-6	45.000004	1
TATCGTG	30	2.1655778E-6	45.000004	1
GTCGAAC	25	3.8909046E-5	45.0	31
GGTCGAA	25	3.8909046E-5	45.0	30
CCTGTCG	20	7.033405E-4	45.0	8
TAGCCGT	50	2.1827873E-11	45.0	44
TCGTATA	20	7.033405E-4	45.0	42
TCCGTAG	45	3.8562575E-10	45.0	1
CGCAACA	20	7.033405E-4	45.0	44
CAATCCA	20	7.033405E-4	45.0	28
ATACCGG	20	7.033405E-4	45.0	2
TGCGTAG	25	3.8909046E-5	45.0	1
CTTACGA	20	7.033405E-4	45.0	34
TATGACG	65	0.0	44.999996	1
CGGTAGT	110	0.0	42.954544	12
ATACTAT	615	0.0	41.341465	45
GCGATAT	170	0.0	41.02941	9
TATCACG	55	6.184564E-11	40.909092	1
CGTTTTT	6400	0.0	40.289062	1
>>END_MODULE