FastQCFastQC Report
Sat 18 Jun 2016
SRR3549029_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549029_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences322734
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38601.1960314066692694No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC7580.2348683435894576No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG4110.12734945806763465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3560.1103075597860777No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3480.10782873821785124No Hit
AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG3370.10442035856153985No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA207.025738E-445.013
ACACGTC207.025738E-445.041
ACCGAAT207.025738E-445.043
TCGATAG207.025738E-445.01
ACGGGCC207.025738E-445.05
GTAGCGA207.025738E-445.038
CTACGGG700.045.03
AACGAGC207.025738E-445.015
TCCGTTA207.025738E-445.024
ACGTCGG207.025738E-445.043
ACGTATG253.88455E-545.01
ATAACGC207.025738E-445.011
CCGCCTC207.025738E-445.031
GGTTACG253.88455E-545.01
CGAGGGT302.160632E-644.9999964
CGTTTTT30350.042.7018131
TTGTTAG750.042.0000041
TAGGGTC700.041.7857135
GCGTAGG650.041.5384642
TAAGGGC1800.041.2500044