##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR3549029_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 322734 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.724866298561665 31.0 31.0 33.0 30.0 34.0 2 32.10763043249239 31.0 31.0 34.0 31.0 34.0 3 32.169879839124484 33.0 31.0 34.0 30.0 34.0 4 35.96361089937844 37.0 35.0 37.0 35.0 37.0 5 35.9398111137965 37.0 35.0 37.0 35.0 37.0 6 36.00482750500412 37.0 35.0 37.0 35.0 37.0 7 36.08217293498671 37.0 35.0 37.0 35.0 37.0 8 35.992284667868894 37.0 35.0 37.0 35.0 37.0 9 37.76699696964063 39.0 38.0 39.0 35.0 39.0 10 37.56198913036742 39.0 37.0 39.0 35.0 39.0 11 37.39676327873729 39.0 37.0 39.0 35.0 39.0 12 36.92285597426983 39.0 35.0 39.0 33.0 39.0 13 36.595614344940415 39.0 35.0 39.0 33.0 39.0 14 37.88229315783277 40.0 35.0 41.0 33.0 41.0 15 38.043983590201215 40.0 35.0 41.0 33.0 41.0 16 38.02635916885113 40.0 35.0 41.0 34.0 41.0 17 38.005007219567815 40.0 35.0 41.0 34.0 41.0 18 37.89507768007089 39.0 36.0 41.0 34.0 41.0 19 37.7532611996257 39.0 36.0 41.0 34.0 41.0 20 37.53782371860417 39.0 35.0 41.0 34.0 41.0 21 37.440833627693394 39.0 35.0 41.0 33.0 41.0 22 37.37454064337814 39.0 35.0 41.0 33.0 41.0 23 37.276462969504294 39.0 35.0 41.0 33.0 41.0 24 37.15693419348442 38.0 35.0 41.0 33.0 41.0 25 37.02956614425502 38.0 35.0 41.0 33.0 41.0 26 36.94560845773919 38.0 35.0 41.0 33.0 41.0 27 36.8575049421505 38.0 35.0 41.0 33.0 41.0 28 36.878354930066244 38.0 35.0 41.0 33.0 41.0 29 36.88153711725446 38.0 35.0 41.0 33.0 41.0 30 36.744913767994696 38.0 35.0 41.0 33.0 41.0 31 36.5389763706334 38.0 35.0 41.0 32.0 41.0 32 36.21791630258975 38.0 35.0 41.0 31.0 41.0 33 35.852265333060664 38.0 35.0 41.0 30.0 41.0 34 35.5529414316434 38.0 35.0 41.0 28.0 41.0 35 35.28992297061977 38.0 35.0 41.0 26.0 41.0 36 35.138138528943344 38.0 35.0 40.0 24.0 41.0 37 35.05441013342257 38.0 35.0 40.0 24.0 41.0 38 34.99425223248868 38.0 35.0 40.0 24.0 41.0 39 34.92605055556589 38.0 35.0 40.0 23.0 41.0 40 34.76933945602261 38.0 34.0 40.0 23.0 41.0 41 34.674192988653196 38.0 34.0 40.0 23.0 41.0 42 34.620461432634926 37.0 34.0 40.0 23.0 41.0 43 34.54767083728395 37.0 34.0 40.0 23.0 41.0 44 34.44671773039097 37.0 34.0 40.0 22.0 41.0 45 34.380220243296336 37.0 34.0 40.0 23.0 41.0 46 34.30254946798292 37.0 34.0 40.0 22.0 41.0 47 34.24563262624948 37.0 34.0 40.0 22.0 41.0 48 34.172959774923 36.0 34.0 40.0 22.0 41.0 49 34.14602428005726 36.0 34.0 40.0 21.0 41.0 50 34.07652432033811 36.0 34.0 40.0 21.0 41.0 51 33.927206306122066 36.0 34.0 40.0 20.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 12 2.0 13 2.0 14 1.0 15 6.0 16 19.0 17 53.0 18 106.0 19 203.0 20 381.0 21 581.0 22 902.0 23 1504.0 24 2546.0 25 4165.0 26 6154.0 27 6968.0 28 6642.0 29 5786.0 30 5938.0 31 6926.0 32 8683.0 33 12355.0 34 24914.0 35 41626.0 36 22044.0 37 29562.0 38 49331.0 39 85293.0 40 41.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.900884319594464 21.842136248427497 26.839750382668083 19.41722904930996 2 36.3956075281811 24.45884226638656 26.95470573289458 12.190844472537755 3 27.949952592537507 24.351943086256796 35.12892970681738 12.56917461438832 4 26.218805579827347 26.659106260883576 33.43465516493459 13.687432994354484 5 22.318689694919037 30.720965253118663 33.13192908091494 13.828415971047365 6 20.659428507687448 38.8725699802314 31.35461401649656 9.113387495584599 7 80.78665402467668 4.871813939653089 12.569794319780375 1.7717377158898662 8 81.32362874689373 4.5759046149460545 12.32903877496638 1.7714278631938376 9 75.9402480060979 6.553074668302688 14.250435343037921 3.2562419825614906 10 41.88061995327421 24.995816988603618 20.844410567216347 12.279152490905823 11 36.70050258107296 22.798651521066883 27.19732039388475 13.303525503975411 12 34.44415524859482 21.09477154560722 30.892933499414376 13.568139706383587 13 20.31611172048808 32.85306165448946 30.270749285789535 16.56007733923293 14 15.808994404060309 35.337770423940455 32.53298381949221 16.320251352507018 15 15.668631132759486 23.654154814801043 45.80645361195288 14.870760440486594 16 17.28296367906697 19.252077562326868 44.99309028487857 18.47186847372759 17 17.666871169446043 20.500164221928895 33.66890380313199 28.16406080549307 18 20.13856612566386 23.69164699102047 37.26412463514845 18.90566224816722 19 28.361127120167072 22.756201701711007 30.666741031313716 18.21593014680821 20 29.05023951613403 21.738025742561987 31.431147632415552 17.780587108888433 21 22.31218278830244 26.21012970433856 32.12118958647059 19.356497920888412 22 22.168411137345306 24.75506144378962 29.790477606945657 23.286049811919412 23 20.50078392732095 27.939727453568576 30.53040584506126 21.02908277404922 24 19.06678565010194 22.86186147105666 40.819684322073286 17.251668556768113 25 18.59797852101111 25.193812861365707 37.171478679035985 19.036729938587197 26 17.603351366760243 31.310924786356566 31.63193217944189 19.453791667441298 27 18.673892431538047 31.036705150371514 32.478139892295204 17.81126252579524 28 16.961956285981643 27.984656094492678 38.22497784553223 16.828409773993442 29 17.924358759845568 24.95119820037554 37.041650399400126 20.082792640378763 30 18.480234496520353 29.879405330705783 32.922468658399794 18.717891514374067 31 24.809285665594576 27.56480569137432 29.823941698116712 17.801966944914387 32 26.479701549883185 26.312071241331868 30.739246562184334 16.468980646600606 33 24.613148909008657 28.68616259830077 28.42278780667671 18.27790068601387 34 19.98673830460999 27.701140877626777 30.818258999671556 21.493861818091677 35 21.204769252697268 25.754336388480915 32.25783462541907 20.78305973340274 36 25.562537569639392 29.113449466123804 26.966170282647628 18.357842681589172 37 20.306816139607232 33.005509180935384 29.871659013305074 16.81601566615231 38 20.39853253763161 32.43135213519493 26.81217349272156 20.3579418344519 39 21.423835108789284 31.19473002534595 28.454082928975566 18.927351936889202 40 23.27365570407828 27.241009624024738 29.136688418325928 20.34864625357105 41 17.761995947126735 27.504074562952773 30.274467518141872 24.459461971778616 42 20.73472271282232 28.177074618726255 26.686683150830092 24.401519517621324 43 20.433545892282808 28.957903412717595 28.119751869961018 22.48879882503858 44 19.617084038248215 30.178103329677068 29.908531484132446 20.29628114794227 45 18.40586984947356 34.5216184226019 26.361027967304345 20.7114837606202 46 19.71933542793756 33.8080276636487 27.208475090941764 19.264161817471972 47 21.41144100094815 28.890355524983423 29.049000105349915 20.64920336871851 48 21.126686373298135 26.31609932638024 31.869589197295607 20.687625103026022 49 19.63629490540197 28.36732417408764 32.17076601783512 19.825614902675266 50 18.782031022451925 32.482787682735626 29.17480029993741 19.560380994875036 51 18.25497158650777 32.79945713807656 27.57750965191148 21.368061623504186 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 525.0 1 724.5 2 924.0 3 4134.5 4 7345.0 5 5330.0 6 3315.0 7 3306.5 8 3298.0 9 3417.5 10 3537.0 11 3480.0 12 3423.0 13 3335.0 14 3247.0 15 3050.0 16 2853.0 17 2667.5 18 2482.0 19 2432.0 20 2382.0 21 2358.0 22 2334.0 23 2447.5 24 2561.0 25 2828.5 26 3266.0 27 3436.0 28 4186.0 29 4936.0 30 5533.5 31 6131.0 32 7193.5 33 8256.0 34 9075.5 35 9895.0 36 10331.5 37 10768.0 38 12064.5 39 13361.0 40 16652.5 41 19944.0 42 22840.0 43 25736.0 44 25669.0 45 25602.0 46 24461.0 47 23320.0 48 21574.0 49 19828.0 50 19088.0 51 18348.0 52 17403.5 53 16459.0 54 15872.0 55 15285.0 56 14791.0 57 14297.0 58 13648.5 59 13000.0 60 12633.0 61 12266.0 62 9962.0 63 7658.0 64 6243.5 65 4829.0 66 3923.0 67 3017.0 68 2426.0 69 1835.0 70 1539.0 71 1243.0 72 1039.5 73 836.0 74 703.5 75 423.0 76 275.0 77 209.0 78 143.0 79 111.5 80 80.0 81 58.5 82 37.0 83 22.5 84 8.0 85 6.5 86 5.0 87 4.0 88 3.0 89 2.0 90 1.0 91 1.0 92 1.0 93 1.5 94 2.0 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 322734.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 28.66757143653907 #Duplication Level Percentage of deduplicated Percentage of total 1 66.29161262429744 19.004195405504223 2 7.715088629485517 4.423457088500127 3 4.399048854301773 3.783301418505643 4 3.04798962386511 3.495138411199316 5 2.2632944228274967 3.2441577274163866 6 1.922827496757458 3.307367677406161 7 1.5931690445309121 3.1970601176200835 8 1.3391699092088196 3.0712599230325903 9 1.1867704280155642 3.061964342151741 >10 10.158884565499351 49.87822789046088 >50 0.06376999567661046 1.1641165789783536 >100 0.016212710765239946 0.9388536689657736 >500 0.00108084738434933 0.2348683435894576 >1k 0.00108084738434933 1.1960314066692694 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3860 1.1960314066692694 No Hit GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC 758 0.2348683435894576 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 411 0.12734945806763465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 356 0.1103075597860777 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 348 0.10782873821785124 No Hit AAGGAAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 337 0.10442035856153985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.003718232352339698 0.0 2 0.0 0.0 0.0 0.015492634801415408 0.0 3 0.0 0.0 0.0 0.024478362986236343 0.0 4 0.0 0.0 0.0 0.037492176219425286 0.0 5 0.0 0.0 0.0 0.05825230685332193 0.0 6 0.0 0.0 0.0 0.09047698724026598 0.0 7 0.0 0.0 0.0 0.10565976934565308 0.0 8 0.0 0.0 0.0 0.15120811566181439 0.0 9 0.0 0.0 0.0 0.16948942472748454 0.0 10 0.0 0.0 0.0 0.20450277937868339 0.0 11 0.0 0.0 0.0 0.2432343663822219 0.0 12 0.0 0.0 0.0 0.2770083102493075 0.0 13 0.0 0.0 0.0 0.29188123965866625 0.0 14 0.0 0.0 0.0 0.30148667323554385 0.0 15 0.0 0.0 0.0 0.3114019595084497 0.0 16 0.0 0.0 0.0 0.3284438577900066 0.0 17 0.0 0.0 0.0 0.35942912739283744 0.0 18 0.0 0.0 0.0 0.38917498621155505 0.0 19 0.0 0.0 0.0 0.4068365898851686 0.0 20 0.0 0.0 0.0 0.4238784881667255 0.0 21 0.0 0.0 0.0 0.44463861880062217 0.0 22 0.0 0.0 0.0 0.4743844776193398 0.0 23 0.0 0.0 0.0 0.5013416621738026 0.0 24 0.0 0.0 0.0 0.5264397305520956 0.0 25 0.0 0.0 0.0 0.5459604504018789 0.0 26 0.0 0.0 0.0 0.566100875643719 0.0 27 0.0 0.0 0.0 0.587170858973644 0.0 28 0.0 0.0 0.0 0.6039029045591726 0.0 29 0.0 0.0 0.0 0.6280714148493806 0.0 30 0.0 0.0 0.0 0.6599862425402964 0.0 31 0.0 0.0 0.0 0.6909715121431271 0.0 32 0.0 0.0 0.0 0.7176188440015616 0.0 33 0.0 0.0 0.0 0.7489139663004207 0.0 34 0.0 0.0 0.0 0.7811386466873649 0.0 35 0.0 0.0 0.0 0.8229687606511864 0.0 36 0.0 0.0 0.0 0.8573624099103286 0.0 37 0.0 0.0 0.0 0.8954742915218106 0.0 38 0.0 0.0 0.0 0.9276989719087546 0.0 39 0.0 0.0 0.0 0.9667404116083214 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACAACGA 20 7.025738E-4 45.0 13 ACACGTC 20 7.025738E-4 45.0 41 ACCGAAT 20 7.025738E-4 45.0 43 TCGATAG 20 7.025738E-4 45.0 1 ACGGGCC 20 7.025738E-4 45.0 5 GTAGCGA 20 7.025738E-4 45.0 38 CTACGGG 70 0.0 45.0 3 AACGAGC 20 7.025738E-4 45.0 15 TCCGTTA 20 7.025738E-4 45.0 24 ACGTCGG 20 7.025738E-4 45.0 43 ACGTATG 25 3.88455E-5 45.0 1 ATAACGC 20 7.025738E-4 45.0 11 CCGCCTC 20 7.025738E-4 45.0 31 GGTTACG 25 3.88455E-5 45.0 1 CGAGGGT 30 2.160632E-6 44.999996 4 CGTTTTT 3035 0.0 42.701813 1 TTGTTAG 75 0.0 42.000004 1 TAGGGTC 70 0.0 41.785713 5 GCGTAGG 65 0.0 41.538464 2 TAAGGGC 180 0.0 41.250004 4 >>END_MODULE