##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549028_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	773190
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96288493125881	31.0	31.0	33.0	30.0	34.0
2	31.250895640140197	31.0	31.0	34.0	30.0	34.0
3	31.32496152304091	31.0	31.0	34.0	28.0	34.0
4	35.15167940609682	37.0	35.0	37.0	32.0	37.0
5	35.02691576455981	37.0	35.0	37.0	32.0	37.0
6	35.07296524786922	37.0	35.0	37.0	32.0	37.0
7	35.57922244209056	37.0	35.0	37.0	35.0	37.0
8	35.51733855843971	37.0	35.0	37.0	35.0	37.0
9	37.244943674905265	39.0	37.0	39.0	34.0	39.0
10	36.68268472173722	39.0	37.0	39.0	32.0	39.0
11	36.30661157024793	38.0	35.0	39.0	32.0	39.0
12	35.01608272222869	35.0	34.0	39.0	30.0	39.0
13	34.33662747836883	35.0	33.0	39.0	25.0	39.0
14	35.241755584009105	36.0	33.0	40.0	26.0	41.0
15	35.75914975620481	36.0	33.0	40.0	30.0	41.0
16	35.95665619058705	36.0	34.0	40.0	31.0	41.0
17	35.897114551403924	36.0	34.0	40.0	31.0	41.0
18	35.82790646542247	36.0	34.0	40.0	30.0	41.0
19	35.69294222636092	36.0	34.0	40.0	30.0	41.0
20	35.48481356458309	35.0	34.0	40.0	30.0	41.0
21	35.26614674271524	35.0	34.0	40.0	29.0	41.0
22	35.15720456808805	35.0	34.0	40.0	29.0	41.0
23	35.17977728630738	35.0	34.0	40.0	29.0	41.0
24	35.038465319003095	35.0	34.0	40.0	29.0	41.0
25	34.90087947335066	35.0	34.0	39.0	29.0	41.0
26	34.71800333682536	35.0	34.0	39.0	29.0	41.0
27	34.56753191324254	35.0	33.0	39.0	27.0	41.0
28	34.63649426402307	35.0	33.0	39.0	27.0	41.0
29	34.76418862116685	35.0	34.0	39.0	29.0	41.0
30	34.67022982708002	35.0	34.0	39.0	28.0	41.0
31	34.395382764909016	35.0	33.0	39.0	27.0	41.0
32	34.08372974301271	35.0	33.0	39.0	25.0	41.0
33	33.77027768077704	35.0	33.0	39.0	23.0	41.0
34	33.57690347779977	35.0	33.0	39.0	23.0	41.0
35	33.36316688006829	35.0	33.0	39.0	23.0	41.0
36	33.08664752518786	35.0	32.0	39.0	21.0	41.0
37	33.027182193251335	35.0	32.0	39.0	21.0	41.0
38	33.09407131494199	35.0	32.0	39.0	22.0	41.0
39	33.06823549192307	35.0	32.0	39.0	21.0	41.0
40	32.8576507714792	35.0	32.0	39.0	20.0	41.0
41	32.862957358475924	35.0	32.0	39.0	20.0	41.0
42	32.81930444004708	35.0	32.0	39.0	20.0	41.0
43	32.663596269998315	35.0	32.0	38.0	19.0	41.0
44	32.59694253676328	35.0	31.0	38.0	18.0	41.0
45	32.56255254206599	35.0	31.0	38.0	20.0	40.0
46	32.53733623042202	35.0	31.0	38.0	20.0	40.0
47	32.472490590928494	35.0	31.0	38.0	19.0	40.0
48	32.460220644343565	35.0	32.0	38.0	18.0	40.0
49	32.555485715024766	35.0	32.0	38.0	18.0	40.0
50	32.43250171367969	35.0	32.0	38.0	18.0	40.0
51	32.21053816009002	35.0	31.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	11.0
15	39.0
16	125.0
17	342.0
18	776.0
19	1478.0
20	2410.0
21	3781.0
22	5556.0
23	7754.0
24	10743.0
25	14761.0
26	18825.0
27	21863.0
28	22715.0
29	23956.0
30	27852.0
31	32515.0
32	40238.0
33	52492.0
34	90525.0
35	125225.0
36	47823.0
37	53251.0
38	70333.0
39	97747.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.111757782692486	22.933431627413704	28.00333682535987	17.951473764533944
2	32.28042266454558	25.601081234884056	29.63424255357674	12.484253546993623
3	27.567609513832302	24.42582030290097	34.60675901136849	13.399811171898241
4	24.872023694046742	28.190095578059726	32.73658479804447	14.201295929849067
5	22.426829110567905	31.683932797889263	31.990455127458965	13.89878296408386
6	20.38101889574361	39.33172958781153	30.30548765503951	9.981763861405346
7	83.4295580646413	5.1727259793841105	10.016425458166815	1.3812904978077833
8	85.34965532404712	3.874985449889419	9.208215315769733	1.5671439102937181
9	81.59559745987403	5.072750552904202	10.849597123604807	2.4820548636169635
10	58.59348931051876	17.143910293718232	15.070939872476362	9.191660523286643
11	54.642325948343874	17.174562526675203	18.741835771285196	9.441275753695729
12	47.63460468966231	20.310661027690475	22.127937505658377	9.926796776988839
13	19.80522251969115	46.70908832240458	22.33409640579935	11.151592752104916
14	13.087986135361296	47.72746672874714	27.514453109843632	11.67009402604793
15	12.263997206378768	20.718581461219106	56.54845510159211	10.468966230810022
16	13.378600344029282	15.025155524515318	54.40137611712515	17.194868014330243
17	14.086447056997633	15.780985268821377	30.788292657690864	39.34427501649013
18	20.84688110296305	21.69570222067021	38.40220385674931	19.055212819617427
19	31.726483787943454	21.86732885836599	27.638355384834256	18.767831968856296
20	33.992291674750064	20.837310363558764	26.25383152911962	18.916566432571553
21	20.34920265394017	29.93973020861625	29.68481227123993	20.02625486620365
22	21.905223813034315	24.562268006570186	26.058278042913123	27.47423013748238
23	18.68415266622693	32.09198256573417	25.68204451687166	23.54182025116724
24	18.72321162974172	21.95799221407416	44.611027043805535	14.707769112378589
25	14.578176127471904	23.897101617972297	41.719629069180925	19.805093185374876
26	13.68990804330113	37.816578072660015	29.79358243122648	18.699931452812375
27	16.68955884064719	36.68464413662877	30.615372676832344	16.010424345891696
28	11.907163827778424	29.369236539531034	44.24449359148463	14.479106041205913
29	13.424126023357777	23.951292696491162	42.5634061485534	20.06117513159767
30	16.119323840194518	32.52551119388507	33.9265898420828	17.428575123837607
31	30.86628125040417	26.274913022672308	25.887427411114995	16.971378315808533
32	32.82116944088775	25.916010295011578	27.857447716602646	13.405372547498029
33	29.743788719460934	27.637062041671516	25.034338260970785	17.584810977896765
34	18.34400341442595	27.987687373090704	28.748690490047725	24.919618722435626
35	19.379324616200417	24.72535857939187	33.56846312031971	22.326853684088
36	32.44803993843687	24.864263635070294	26.33414813952586	16.353548286966983
37	19.31349344921688	34.723677233280306	29.686881620300316	16.275947697202497
38	19.251800980354115	35.593321175907604	23.776432700888527	21.378445142849753
39	19.79591044891941	34.361282479080174	27.46582340692456	18.376983665075855
40	24.666640799803414	26.591523428911394	24.88068909323711	23.861146678048087
41	15.60353858689326	24.54041050711985	27.647796789922268	32.208254116064616
42	21.346887569678863	25.828062959945164	23.986859633466548	28.83818983690943
43	21.782356212573884	26.22731799428342	25.549218174058126	26.44110761908457
44	18.77546269351647	31.617972296589453	29.691020318421085	19.915544691472988
45	16.08246356005639	41.41401207982514	21.469108498557922	21.03441586156055
46	21.09481498726057	36.84023331910656	24.039369365873846	18.025582327759025
47	21.352319610962375	27.891462641782745	26.4444703113077	24.31174743594718
48	23.55746970343641	22.84703630414258	33.361657548597364	20.233836443823638
49	20.37519885151127	23.582948563742416	34.67595287057515	21.36589971417116
50	18.375431653280565	36.551559125182685	25.689804575848108	19.38320464568864
51	16.481201257129555	37.734968119091036	23.32738395478472	22.456446668994683
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	847.0
1	1058.0
2	1269.0
3	5462.5
4	9656.0
5	7246.5
6	4837.0
7	5328.0
8	5819.0
9	6310.0
10	6801.0
11	6844.5
12	6888.0
13	6736.5
14	6585.0
15	6101.5
16	5618.0
17	5183.5
18	4749.0
19	4382.0
20	4015.0
21	3752.0
22	3489.0
23	3645.5
24	3802.0
25	3859.5
26	4374.5
27	4832.0
28	6257.5
29	7683.0
30	8806.5
31	9930.0
32	11327.0
33	12724.0
34	14701.0
35	16678.0
36	17105.0
37	17532.0
38	23864.0
39	30196.0
40	44860.5
41	59525.0
42	76805.0
43	94085.0
44	92882.0
45	91679.0
46	82634.0
47	73589.0
48	64275.5
49	54962.0
50	53341.5
51	51721.0
52	47266.0
53	42811.0
54	38459.5
55	34108.0
56	30548.5
57	26989.0
58	25593.0
59	24197.0
60	21031.0
61	17865.0
62	16358.5
63	14852.0
64	11523.0
65	8194.0
66	6295.5
67	4397.0
68	3491.0
69	2585.0
70	2094.0
71	1603.0
72	1234.5
73	866.0
74	722.5
75	437.5
76	296.0
77	247.0
78	198.0
79	160.5
80	123.0
81	96.0
82	69.0
83	41.5
84	14.0
85	13.0
86	12.0
87	8.0
88	4.0
89	2.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	773190.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	20.842012847327435
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.61248187069347	17.21810408498598
2	5.67293648925139	2.364708303821001
3	1.732771149746001	1.0834331569345343
4	0.9289893377704377	0.7744803085136667
5	0.5855293751335708	0.6101805379510744
6	0.4142853097908558	0.5180723849472025
7	0.32976460626672977	0.4811070712283537
8	0.27387010583189625	0.45664034113978447
9	0.2593688916421061	0.4865192794621667
>10	4.271884819013887	22.416105101468286
>50	2.1845579838590408	32.687160835516124
>100	0.7304596380885466	19.5952112233882
>500	0.0018602537472543633	0.25395288287214707
>1k	6.200845824181211E-4	0.3563094646709972
>5k	6.200845824181211E-4	0.6980150231004918
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5401	0.698534642196614	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2757	0.3565747099677957	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.007889393292722358	0.0
2	0.0	0.0	0.0	0.03298025064990494	0.0
3	0.0	0.0	0.0	0.04668968817496346	0.0
4	0.0	0.0	0.0	0.07553124070409602	0.0
5	0.0	0.0	0.0	0.11588354738162676	0.0
6	0.0	0.0	0.0	0.1795160309885022	0.0
7	0.0	0.0	0.0	0.212366947322133	0.0
8	0.0	0.0	0.0	0.3008316196536427	0.0
9	0.0	0.0	0.0	0.3405372547498028	0.0
10	0.0	0.0	0.0	0.3944696646361179	0.0
11	0.0	0.0	0.0	0.45693813939652606	0.0
12	0.0	0.0	0.0	0.5036278275714895	0.0
13	1.2933431627413703E-4	0.0	0.0	0.5249679897567221	0.0
14	1.2933431627413703E-4	0.0	0.0	0.5358320723237496	0.0
15	1.2933431627413703E-4	0.0	0.0	0.5476014951046961	0.0
16	1.2933431627413703E-4	0.0	0.0	0.5761843790012804	0.0
17	1.2933431627413703E-4	0.0	0.0	0.6070952805907992	0.0
18	1.2933431627413703E-4	0.0	0.0	0.6533969658169402	0.0
19	1.2933431627413703E-4	0.0	0.0	0.6738317877882538	0.0
20	1.2933431627413703E-4	0.0	0.0	0.696594627452502	0.0
21	1.2933431627413703E-4	0.0	0.0	0.7254361799816346	0.0
22	1.2933431627413703E-4	0.0	0.0	0.7549244040921378	0.0
23	1.2933431627413703E-4	0.0	0.0	0.7912673469651703	0.0
24	1.2933431627413703E-4	0.0	0.0	0.819332893596658	0.0
25	1.2933431627413703E-4	0.0	0.0	0.8370516949262148	0.0
26	1.2933431627413703E-4	0.0	0.0	0.8640825670275094	0.0
27	1.2933431627413703E-4	0.0	0.0	0.8874920782731283	0.0
28	1.2933431627413703E-4	0.0	0.0	0.9132296072116814	0.0
29	1.2933431627413703E-4	0.0	0.0	0.9397431420478796	0.0
30	1.2933431627413703E-4	0.0	0.0	0.9772500937673793	0.0
31	1.2933431627413703E-4	0.0	0.0	1.0159210543333463	0.0
32	1.2933431627413703E-4	0.0	0.0	1.0481252990856065	0.0
33	1.2933431627413703E-4	0.0	0.0	1.078001526144932	0.0
34	2.5866863254827405E-4	0.0	0.0	1.1160258151295284	0.0
35	2.5866863254827405E-4	0.0	0.0	1.1738382545040675	0.0
36	2.5866863254827405E-4	0.0	0.0	1.2188465965674673	0.0
37	2.5866863254827405E-4	0.0	0.0	1.2619149238867549	0.0
38	2.5866863254827405E-4	0.0	0.0	1.3022672305642857	0.0
39	2.5866863254827405E-4	0.0	0.0	1.344947554934751	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGGAT	20	7.031851E-4	45.000004	42
CGTGGTA	20	7.031851E-4	45.000004	3
ACGCCGA	20	7.031851E-4	45.000004	12
CGCGGTA	20	7.031851E-4	45.000004	32
CCGATTC	20	7.031851E-4	45.000004	11
CACGGAA	20	7.031851E-4	45.000004	11
CCGTGTT	20	7.031851E-4	45.000004	23
GCACGGT	30	2.1645756E-6	45.000004	3
TGTATCG	20	7.031851E-4	45.000004	1
TAACGCG	20	7.031851E-4	45.000004	1
CCTAAAC	20	7.031851E-4	45.000004	20
TAGCCGT	30	2.1645756E-6	45.000004	44
ACGATAT	30	2.1645756E-6	45.000004	38
GCCGACC	20	7.031851E-4	45.000004	14
TGCGCAC	20	7.031851E-4	45.000004	12
CATAGTA	20	7.031851E-4	45.000004	31
TACGATA	30	2.1645756E-6	45.000004	37
ACTACGA	30	2.1645756E-6	45.000004	35
ACTCGAA	20	7.031851E-4	45.000004	13
GTTCACG	40	6.8102963E-9	45.000004	1
>>END_MODULE