##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549026_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1344263
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.871949164709584	31.0	31.0	33.0	28.0	34.0
2	31.15674016170943	31.0	31.0	34.0	28.0	34.0
3	31.235192071789523	31.0	31.0	34.0	28.0	34.0
4	35.08472895556896	35.0	35.0	37.0	32.0	37.0
5	34.95717504684723	35.0	35.0	37.0	32.0	37.0
6	35.01325112719758	37.0	35.0	37.0	32.0	37.0
7	35.517087802014935	37.0	35.0	37.0	35.0	37.0
8	35.43031534751756	37.0	35.0	37.0	35.0	37.0
9	37.12231981390546	39.0	37.0	39.0	34.0	39.0
10	36.51183957305974	38.0	35.0	39.0	32.0	39.0
11	36.1822604654	38.0	35.0	39.0	31.0	39.0
12	35.4750707264873	37.0	35.0	39.0	30.0	39.0
13	35.04430457432809	37.0	34.0	39.0	29.0	39.0
14	36.04599471978326	38.0	34.0	40.0	30.0	41.0
15	36.376448656252535	38.0	35.0	40.0	30.0	41.0
16	36.42880448245619	38.0	35.0	40.0	31.0	41.0
17	36.32516256119524	38.0	34.0	40.0	31.0	41.0
18	36.23079709848445	38.0	35.0	40.0	30.0	41.0
19	36.122410569955434	37.0	35.0	40.0	30.0	41.0
20	35.96794600461368	37.0	34.0	40.0	30.0	41.0
21	35.79292668175796	37.0	34.0	40.0	30.0	41.0
22	35.6856842745802	37.0	34.0	40.0	30.0	41.0
23	35.60053203874539	36.0	34.0	40.0	29.0	41.0
24	35.49584195949751	36.0	34.0	40.0	29.0	41.0
25	35.334043263855364	36.0	34.0	40.0	29.0	41.0
26	35.16163652499548	36.0	34.0	40.0	28.0	41.0
27	35.072865205692636	36.0	34.0	40.0	27.0	41.0
28	35.08811891720593	36.0	34.0	40.0	27.0	41.0
29	35.112832087173416	36.0	34.0	40.0	28.0	41.0
30	34.971887941570955	36.0	34.0	40.0	27.0	41.0
31	34.802383908506	36.0	33.0	40.0	27.0	41.0
32	34.563483485002564	36.0	33.0	40.0	25.0	41.0
33	34.27992736540394	36.0	33.0	40.0	24.0	41.0
34	34.07499797286692	36.0	33.0	40.0	23.0	41.0
35	33.85478213712644	35.0	33.0	40.0	23.0	41.0
36	33.66254966476054	35.0	33.0	40.0	22.0	41.0
37	33.61123009411105	35.0	33.0	40.0	22.0	41.0
38	33.58045709805298	35.0	32.0	40.0	22.0	41.0
39	33.49743539768632	35.0	32.0	40.0	21.0	41.0
40	33.32561634144509	35.0	32.0	40.0	21.0	41.0
41	33.25778214530936	35.0	32.0	40.0	20.0	41.0
42	33.202344332916994	35.0	32.0	40.0	20.0	41.0
43	33.09372942645896	35.0	32.0	39.0	20.0	41.0
44	32.98528338576603	35.0	32.0	39.0	19.0	41.0
45	32.93031497556654	35.0	31.0	39.0	20.0	41.0
46	32.855006051643166	35.0	31.0	39.0	20.0	41.0
47	32.758853736210845	35.0	31.0	39.0	20.0	41.0
48	32.72216895056994	35.0	31.0	39.0	19.0	41.0
49	32.73890972227905	35.0	31.0	39.0	19.0	41.0
50	32.643750516082044	35.0	31.0	39.0	18.0	41.0
51	32.46508830489272	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	3.0
11	1.0
12	6.0
13	12.0
14	20.0
15	76.0
16	254.0
17	613.0
18	1363.0
19	2565.0
20	4320.0
21	6568.0
22	9429.0
23	13282.0
24	17874.0
25	24040.0
26	30119.0
27	34670.0
28	37349.0
29	40816.0
30	47352.0
31	55547.0
32	67187.0
33	83565.0
34	129920.0
35	166447.0
36	101253.0
37	119075.0
38	155402.0
39	195073.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.96140710560359	22.85624167294644	26.9762687807371	17.206082440712866
2	33.93398464437391	24.874299151282152	28.34542050179169	12.846295702552254
3	28.349883914085268	24.635878544600274	33.19171917995214	13.822518361362324
4	25.78476086896686	26.759570113883964	32.36457449174752	15.091094525401653
5	22.29147123739923	30.44419135243624	32.225539198802615	15.038798211361915
6	21.74879469270522	39.082753895629054	29.2558078292715	9.91264358239422
7	83.01954305072744	4.7394743439341855	10.010466701828436	2.230515903509953
8	83.85278773573326	4.258467279096427	9.789974134525758	2.098770850644554
9	79.00061223138627	5.755272591747299	11.700091425561814	3.544023751304618
10	47.43588122264765	21.946598247515553	17.990452761104038	12.627067768732756
11	40.30171179300479	21.90627875646358	23.53884619304407	14.25316325748756
12	36.27504439235477	21.451010702518776	27.759672028464667	14.514272876661785
13	21.3738680600448	34.63466598426052	27.42357708275836	16.567888872936322
14	16.519460849551017	35.978450645446614	30.891202093637926	16.610886411364444
15	16.4132316369639	22.383268750237118	45.538856607672756	15.66464300512623
16	18.49370249720479	18.812836476195507	43.53656985277434	19.15689117382536
17	18.578581720987632	19.377978862767183	31.311729921897722	30.73170949434746
18	22.038544540763226	22.46732968176614	35.41115094293304	20.082974834537588
19	29.067675001097253	23.283539009851495	27.634324533220063	20.014461455831185
20	30.745769243072225	21.98237993606906	28.477686286091338	18.794164534767376
21	23.913624045294707	26.309806935101243	30.03802083372078	19.738548185883268
22	23.201932955083937	23.99530449026716	27.982098741094564	24.82066381355434
23	21.50114970061662	27.45876364967272	27.97979264474288	23.060294004967776
24	20.934742680561765	22.47990162639305	38.41264692995344	18.172708763091748
25	19.496184898342065	24.43004084766151	35.193187642596726	20.880586611399703
26	18.188851437553513	30.89514477449725	29.846986787555707	21.069017000393526
27	18.247768479828725	30.891202093637926	30.429313311457655	20.431716115075695
28	16.98164719255086	27.124454068883843	37.12450614202727	18.76939259653803
29	18.575159771562557	24.228145831582065	35.890967764492515	21.305726632362866
30	19.822757897822076	28.179307174265748	32.53098538009303	19.46694954781914
31	26.840283486192806	26.47264709361189	27.864934168388178	18.822135251807122
32	29.196221275152258	24.588417593878578	28.417058269103592	17.798302861865572
33	27.137397964535214	27.031912653997026	26.969648052501633	18.86104132896613
34	20.837440292561798	27.220119872376163	28.999384793005532	22.9430550420565
35	21.333399788583037	25.73023284878034	31.674902902185064	21.261464460451563
36	27.26958935862997	27.952268268932496	26.08075949423587	18.697382878201662
37	21.399904631757327	32.34597694052429	27.946837783975308	18.307280643743077
38	21.457036309115107	31.606091962659093	26.113342403978983	20.823529324246817
39	21.560066742891827	31.32326040365613	26.89250541002765	20.224167443424392
40	24.12206539940473	25.76713039040723	27.373512474865407	22.737291735322625
41	18.421246437639063	26.075477789688478	28.39474120763571	27.108534565036752
42	21.86633121643607	26.581777524189835	25.264550166150524	26.28734109322357
43	22.012433578845805	26.227605758694537	27.374553937733907	24.38540672472574
44	20.290374725779106	28.38261560423816	29.315543163800534	22.0114665061822
45	18.87026571437286	34.30251371941353	24.72678337497945	22.100437191234157
46	21.473699715011126	32.1024234097048	26.354813009061473	20.069063866222606
47	21.40600462855855	28.18607668291101	27.49030509654733	22.917613591983116
48	22.400080936542924	24.9981588424289	31.290826274322807	21.31093394670537
49	21.674032536787816	25.60994388746845	31.20825314689164	21.50777042885209
50	19.986267568176764	31.24634093179683	27.73661106494786	21.03078043507855
51	18.560058559969292	31.65332974276611	27.038458992027604	22.748152705236997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1673.0
1	2469.5
2	3266.0
3	11733.5
4	20201.0
5	14572.5
6	8944.0
7	9257.5
8	9571.0
9	9841.5
10	10112.0
11	10147.0
12	10182.0
13	9890.5
14	9599.0
15	9329.0
16	9059.0
17	8636.5
18	8214.0
19	8191.0
20	8168.0
21	7825.0
22	7482.0
23	7757.0
24	8032.0
25	8853.5
26	10924.0
27	12173.0
28	14064.5
29	15956.0
30	17930.0
31	19904.0
32	23450.5
33	26997.0
34	30588.5
35	34180.0
36	35524.0
37	36868.0
38	43502.5
39	50137.0
40	64583.5
41	79030.0
42	93946.0
43	108862.0
44	110524.5
45	112187.0
46	107341.5
47	102496.0
48	97149.5
49	91803.0
50	87544.0
51	83285.0
52	80531.0
53	77777.0
54	75089.0
55	72401.0
56	69942.5
57	67484.0
58	64796.0
59	62108.0
60	57508.0
61	52908.0
62	47249.5
63	41591.0
64	34295.5
65	27000.0
66	21830.5
67	16661.0
68	13497.0
69	10333.0
70	8540.0
71	6747.0
72	5744.5
73	4742.0
74	3850.0
75	2272.0
76	1586.0
77	1263.0
78	940.0
79	725.5
80	511.0
81	372.0
82	233.0
83	178.5
84	124.0
85	97.0
86	70.0
87	47.5
88	25.0
89	13.0
90	1.0
91	0.5
92	0.0
93	1.5
94	3.0
95	3.5
96	4.0
97	2.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1344263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.423378210128007
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.21513605100762	16.970558195874823
2	5.583860886181244	2.392503272748027
3	2.260675600452888	1.4529392519673134
4	1.3119554531696382	1.1242607147237218
5	0.9183086165940055	0.9836636403456405
6	0.7155898367085396	0.9198211029078471
7	0.5939607936882207	0.8907252707619394
8	0.5136522225051289	0.8803332664960161
9	0.46609679107852237	0.8986831054021982
>10	5.978065811978344	30.10864616792433
>50	2.0101950976421303	30.336021174899468
>100	0.4300573463661375	11.751012499901293
>500	6.987121793112974E-4	0.09512656688793229
>1k	0.0013974243586225947	0.3979448907499103
>5k	0.0	0.0
>10k+	3.493560896556487E-4	0.7977608784094967
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10659	0.7929251939538617	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	1882	0.14000236560851562	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.439020489294134E-5	0.0	0.0	0.007290240079508251	0.0
2	7.439020489294134E-5	0.0	0.0	0.032954860767573015	0.0
3	7.439020489294134E-5	0.0	0.0	0.05266826506420247	0.0
4	1.4878040978588269E-4	0.0	0.0	0.081159713538199	0.0
5	1.4878040978588269E-4	0.0	0.0	0.1321170038898638	0.0
6	1.4878040978588269E-4	0.0	0.0	0.23023768414365345	0.0
7	1.4878040978588269E-4	0.0	0.0	0.28476570433017945	0.0
8	1.4878040978588269E-4	0.0	0.0	0.43362050432095506	0.0
9	1.4878040978588269E-4	0.0	0.0	0.5126229019172588	0.0
10	1.4878040978588269E-4	0.0	0.0	0.6210838206511672	0.0
11	1.4878040978588269E-4	0.0	0.0	0.7087154820150521	0.0
12	1.4878040978588269E-4	0.0	0.0	0.7825849554737428	0.0
13	1.4878040978588269E-4	0.0	0.0	0.8190361558712841	0.0
14	1.4878040978588269E-4	0.0	0.0	0.8371873658651618	0.0
15	1.4878040978588269E-4	0.0	0.0	0.8548178444247889	0.0
16	1.4878040978588269E-4	0.0	0.0	0.8911202644125442	0.0
17	1.4878040978588269E-4	0.0	0.0	0.9289104884981585	0.0
18	1.4878040978588269E-4	0.0	0.0	0.9925885038865162	0.0
19	2.23170614678824E-4	0.0	0.0	1.0187738560088315	0.0
20	2.9756081957176537E-4	0.0	0.0	1.048678718375794	0.0
21	2.9756081957176537E-4	0.0	0.0	1.083046993036333	0.0
22	2.9756081957176537E-4	0.0	0.0	1.1212091681464118	0.0
23	3.7195102446470666E-4	0.0	0.0	1.1663640225164271	0.0
24	3.7195102446470666E-4	0.0	0.0	1.206088391929258	0.0
25	3.7195102446470666E-4	0.0	0.0	1.242762762941478	0.0
26	3.7195102446470666E-4	0.0	0.0	1.274378600020978	0.0
27	3.7195102446470666E-4	0.0	0.0	1.3103090689842687	0.0
28	3.7195102446470666E-4	0.0	0.0	1.3491407559383841	0.0
29	3.7195102446470666E-4	0.0	0.0	1.390948051088217	0.0
30	3.7195102446470666E-4	0.0	0.0	1.444285827996456	0.0
31	3.7195102446470666E-4	0.0	0.0	1.4962101910117291	0.0
32	3.7195102446470666E-4	0.0	0.0	1.5434479711187468	0.0
33	3.7195102446470666E-4	0.0	0.0	1.5917272140942658	0.0
34	3.7195102446470666E-4	0.0	0.0	1.638295482357247	0.0
35	3.7195102446470666E-4	0.0	0.0	1.7024942291798555	0.0
36	3.7195102446470666E-4	0.0	0.0	1.7566502983419168	0.0
37	3.7195102446470666E-4	0.0	0.0	1.8131868540605522	0.0
38	3.7195102446470666E-4	0.0	0.0	1.871731945311297	0.0
39	3.7195102446470666E-4	0.0	0.0	1.9358563019290125	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGACGG	125	0.0	45.000004	2
ATTACCG	35	1.2121382E-7	45.000004	1
ACCGAAT	25	3.8911592E-5	45.000004	16
ATCGTAC	50	2.1827873E-11	45.000004	29
TATGTCG	25	3.8911592E-5	45.000004	1
CGTCAGT	35	1.2121382E-7	45.000004	42
TTGATCG	20	7.033715E-4	45.0	1
CAACGAC	20	7.033715E-4	45.0	12
CTATCGA	20	7.033715E-4	45.0	33
TAGCGAT	20	7.033715E-4	45.0	28
TAGCCGT	55	6.184564E-11	40.909092	44
CGTACTC	55	6.184564E-11	40.909092	31
CGTTTTT	8410	0.0	40.90666	1
CGGTCTA	155	0.0	40.645164	31
AGCGTAA	50	1.0822987E-9	40.500004	15
GCGCGAC	415	0.0	40.120483	9
ATCGTAG	45	1.9286745E-8	40.0	1
GCTACGA	125	0.0	39.600002	10
ACTAACC	165	0.0	39.545452	14
AGGGCGA	1410	0.0	38.617023	6
>>END_MODULE