FastQCFastQC Report
Sat 18 Jun 2016
SRR3549023_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549023_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4416745
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT173060.39182701287939425No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC120220.2721913988695295No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTA253.8926126E-545.00000420
GTCGAAT207.035469E-445.030
TAATGCG1950.043.8461531
CGTTTTT158000.040.7136081
GCGATCT3100.039.1935469
GTCGACG750.039.01
TAACGCG659.094947E-1238.076921
GGATGCC45050.038.0577138
TACGGGC6800.038.051474
AGGGTAT48400.037.840916
ACGGGTA5650.037.8318565
GATGCCA44450.037.5590559
GCACCGA18900.037.59
TACGGGA6650.037.218054
CGTAAGG7900.037.025322
TAGGGTC14300.036.818185
CGTTGTA800.036.562530
GGGCGAT72650.036.483147
GTAGGGT26200.036.4122124
AGGGCGA41650.036.302526
Produced by FastQC (version 0.11.5)