##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549023_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4416745
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.249562970015248	31.0	31.0	33.0	30.0	34.0
2	31.601509935484163	31.0	31.0	34.0	30.0	34.0
3	31.702881420593673	31.0	31.0	34.0	30.0	34.0
4	35.420207868011396	37.0	35.0	37.0	33.0	37.0
5	35.26270862365837	37.0	35.0	37.0	33.0	37.0
6	35.336754782084995	37.0	35.0	37.0	32.0	37.0
7	35.82675228024258	37.0	35.0	37.0	35.0	37.0
8	35.85743664168975	37.0	35.0	37.0	35.0	37.0
9	37.52626719450636	39.0	37.0	39.0	35.0	39.0
10	36.772995724226774	39.0	37.0	39.0	32.0	39.0
11	36.4767202996777	39.0	35.0	39.0	32.0	39.0
12	35.798518365900684	37.0	35.0	39.0	31.0	39.0
13	35.506151249392936	37.0	35.0	39.0	30.0	39.0
14	36.51324833106734	38.0	35.0	41.0	31.0	41.0
15	36.72414277935448	38.0	35.0	41.0	31.0	41.0
16	36.86160600170488	38.0	35.0	41.0	32.0	41.0
17	36.81102237054664	38.0	35.0	41.0	32.0	41.0
18	36.74152526351419	38.0	35.0	40.0	31.0	41.0
19	36.6992814844416	38.0	35.0	40.0	31.0	41.0
20	36.55155685918023	38.0	35.0	40.0	31.0	41.0
21	36.36987374186194	38.0	35.0	40.0	31.0	41.0
22	36.29189369094208	38.0	35.0	40.0	30.0	41.0
23	36.22535736158642	38.0	35.0	40.0	30.0	41.0
24	36.16938990138665	38.0	35.0	40.0	30.0	41.0
25	36.05237476920221	37.0	34.0	40.0	30.0	41.0
26	35.95375689563242	37.0	34.0	40.0	30.0	41.0
27	35.91976014010318	37.0	34.0	40.0	30.0	41.0
28	35.899992188817784	37.0	34.0	40.0	30.0	41.0
29	35.89519204753727	37.0	35.0	40.0	30.0	41.0
30	35.80821985421391	37.0	34.0	40.0	30.0	41.0
31	35.65599734646216	37.0	34.0	40.0	29.0	41.0
32	35.48274690977179	37.0	34.0	40.0	29.0	41.0
33	35.30523790710127	37.0	34.0	40.0	28.0	41.0
34	35.136210037029535	37.0	34.0	40.0	27.0	41.0
35	35.003859629659395	37.0	34.0	40.0	27.0	41.0
36	34.83319978853205	37.0	34.0	40.0	25.0	41.0
37	34.76043217346711	37.0	33.0	40.0	25.0	41.0
38	34.745053880176464	37.0	33.0	40.0	25.0	41.0
39	34.68084369824384	37.0	33.0	40.0	25.0	41.0
40	34.52975981180711	36.0	33.0	40.0	24.0	41.0
41	34.50570929496722	36.0	33.0	40.0	24.0	41.0
42	34.42992271457827	36.0	33.0	40.0	24.0	41.0
43	34.368565991471094	36.0	33.0	40.0	24.0	41.0
44	34.294309044330156	36.0	33.0	40.0	24.0	41.0
45	34.259418644273104	36.0	33.0	40.0	24.0	41.0
46	34.22352071491562	36.0	33.0	40.0	24.0	41.0
47	34.160378966863604	35.0	33.0	40.0	23.0	41.0
48	34.13125706827086	35.0	33.0	40.0	23.0	41.0
49	34.076446115861344	36.0	33.0	40.0	24.0	41.0
50	33.95829689058345	35.0	33.0	40.0	24.0	41.0
51	33.80314190653977	35.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	4.0
13	8.0
14	29.0
15	130.0
16	413.0
17	1149.0
18	2638.0
19	5125.0
20	8620.0
21	13412.0
22	20389.0
23	28900.0
24	40733.0
25	56959.0
26	74118.0
27	85138.0
28	94118.0
29	107299.0
30	126745.0
31	154700.0
32	190344.0
33	243600.0
34	391836.0
35	590177.0
36	323096.0
37	390374.0
38	545806.0
39	920397.0
40	487.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.915381123429132	24.58491490905633	28.95417326560623	14.545530701908305
2	30.334329919431617	26.379426478096423	29.9791135779856	13.307130024486359
3	27.40547620476165	26.2973298209428	32.228213310933725	14.068980663361819
4	25.089766332446178	27.92923295322687	31.81598665985924	15.165014054467713
5	22.26642018047227	32.570909119725044	30.765756229983847	14.396914469818837
6	20.979703378845734	41.26885296751341	27.653509541528887	10.097934112111973
7	86.15602666669685	4.741636657764937	7.156582505895178	1.945754169643029
8	87.30531194352402	3.65325596112069	6.924714920150472	2.1167171752048173
9	82.39703673180136	5.5139474884785065	8.882921699124582	3.2060940805955513
10	46.20642577282592	22.438945422477413	18.805523071854953	12.54910573284172
11	40.81517044792036	24.306814181031505	20.354039003836537	14.5239763672116
12	35.65195636152868	22.47861264347387	26.535718045755413	15.333712949242031
13	21.04624559488945	38.143066896549385	25.676012538645544	15.134674969915629
14	17.054256018855515	38.425310947315275	28.463947997903432	16.05648503592578
15	15.895778452231223	25.209945333045034	43.48985508558905	15.40442112913469
16	16.765468687913838	21.148243785864928	42.57202985456484	19.51425767165639
17	17.551409465567968	22.33303031983961	29.068624065912797	31.046936148679627
18	21.115753796064748	24.809831674683505	33.984484048773474	20.089930480478273
19	27.170869044964107	25.83123997423442	26.28311120519749	20.71477977560398
20	28.687121398224257	23.6799951095207	27.870705689370794	19.762177802884253
21	21.875566735231487	28.792447832057317	28.27330986959854	21.058675563112654
22	22.644164424253606	25.4630502779762	26.467658875484094	25.42512642228609
23	19.963774227400496	29.83570480070731	26.688636088341074	23.511884883551122
24	19.665047450101827	24.169631708418756	38.30959224496773	17.855728596511685
25	17.718206507280815	26.999158882842455	34.5295234386409	20.75311117123583
26	17.89775049272711	33.354857479886206	28.344629359403818	20.40276266798287
27	18.24692165837059	33.41431302916514	28.685536520673026	19.653228791791243
28	16.60498851529803	29.675881220219868	35.75085272072533	17.968277543756773
29	17.178940599921436	25.398341991670335	34.61469928646548	22.808018121942744
30	18.226046556910124	31.62650322805596	30.18886080133673	19.95858941369719
31	25.663446723775085	28.367927059406874	26.71614503440882	19.252481182409216
32	27.03846384611292	26.895960713149613	27.56604694180896	18.499528498928512
33	25.87289961272385	28.91969085831308	25.52807553979231	19.679333989170757
34	19.164429913884547	28.435669254167944	28.986119868817422	23.413780963130087
35	20.07390057610299	28.040672486186097	30.16314050279108	21.722286434919834
36	27.334066150524876	27.946010014162013	25.714502422032513	19.005421413280597
37	19.782486876647848	33.21989383584518	28.91554753557201	18.082071751934965
38	20.348922113456855	32.64114183635234	26.022398848020433	20.987537202170376
39	20.78578228989901	32.48514460309572	27.07982915019998	19.649243956805297
40	23.29181331500913	27.39929518231186	26.883938284868155	22.424953217810852
41	17.82769890496282	26.2020560390061	28.259385588255604	27.710859467775478
42	20.476278345251988	27.261886298620364	26.403878874601094	25.857956481526557
43	21.14611552172471	27.23584902456447	27.521421318187944	24.096614135522877
44	20.147642664450856	30.072349660213572	28.652072963234236	21.127934712101332
45	17.683225995614418	36.28484324994991	24.497225898257653	21.53470485617802
46	20.48472347848925	33.201758308437554	26.43990087722973	19.873617335843477
47	20.523032233013225	28.872846406120345	27.655162342403738	22.94895901846269
48	21.427952032548855	26.667602499125486	30.644128198481006	21.26031726984465
49	20.66338446072843	26.88287415279805	31.204676747242594	21.249064639230927
50	19.55836254979629	32.604508523811084	27.441679336253284	20.395449590139343
51	18.760897448233937	32.82439896349008	25.517411577983335	22.897292010292645
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2775.0
1	4758.5
2	6742.0
3	19094.5
4	31447.0
5	23955.5
6	16464.0
7	17366.5
8	18269.0
9	19404.0
10	20539.0
11	21535.5
12	22532.0
13	22440.5
14	22349.0
15	22378.0
16	22407.0
17	21551.5
18	20696.0
19	21265.5
20	21835.0
21	21132.0
22	20429.0
23	23262.5
24	26096.0
25	30162.5
26	42093.0
27	49957.0
28	58487.5
29	67018.0
30	79596.0
31	92174.0
32	108577.5
33	124981.0
34	130679.0
35	136377.0
36	160131.5
37	183886.0
38	192745.5
39	201605.0
40	258085.5
41	314566.0
42	363795.0
43	413024.0
44	414310.5
45	415597.0
46	401380.0
47	387163.0
48	368816.0
49	350469.0
50	328975.5
51	307482.0
52	277150.5
53	246819.0
54	221232.5
55	195646.0
56	176073.5
57	156501.0
58	141602.0
59	126703.0
60	115920.0
61	105137.0
62	88916.5
63	72696.0
64	66918.0
65	61140.0
66	50522.5
67	39905.0
68	34176.0
69	28447.0
70	24592.5
71	20738.0
72	17033.5
73	13329.0
74	10658.5
75	6617.5
76	5247.0
77	4042.5
78	2838.0
79	2141.5
80	1445.0
81	998.0
82	551.0
83	490.5
84	430.0
85	237.5
86	45.0
87	28.0
88	11.0
89	11.0
90	11.0
91	7.0
92	3.0
93	4.0
94	5.0
95	3.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4416745.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.641702961833376
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.43446798409656	12.581320561003349
2	7.554059108000315	2.3631669744694586
3	2.610873824296688	1.2251553849142423
4	1.3877369347680424	0.8682627569122744
5	0.8768081385754927	0.6857386229057947
6	0.6101668642966959	0.5726429309089326
7	0.46754531822762363	0.5119243492238644
8	0.36449088350659387	0.45610065056850824
9	0.28441431193974287	0.40038517669101104
>10	2.616444067407874	9.35641351696042
>50	1.0215281317970968	11.689653016469668
>100	1.7417527470096263	55.34369631191004
>500	0.02649170039452764	2.615578156004557
>1k	0.0029272597121025013	0.6585362292665238
>5k	0.0	0.0
>10k+	2.9272597121025016E-4	0.6714253617913648
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	17306	0.39182701287939425	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	12022	0.2721913988695295	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038512524494848585	0.0
2	0.0	0.0	0.0	0.12889582713061315	0.0
3	0.0	0.0	0.0	0.1894607906954103	0.0
4	0.0	0.0	0.0	0.29637210207969894	0.0
5	2.2641107874690523E-5	0.0	0.0	0.514088995402723	0.0
6	2.2641107874690523E-5	0.0	0.0	0.7793295741547226	0.0
7	2.2641107874690523E-5	0.0	0.0	0.9238477657188722	0.0
8	2.2641107874690523E-5	0.0	0.0	1.2611097086202623	0.0
9	2.2641107874690523E-5	0.0	0.0	1.3969337147605307	0.0
10	9.056443149876209E-5	0.0	0.0	1.6233674346153106	0.0
11	9.056443149876209E-5	0.0	0.0	1.832933529103446	0.0
12	9.056443149876209E-5	0.0	0.0	2.0099643515756513	0.0
13	9.056443149876209E-5	0.0	0.0	2.0852913174747467	0.0
14	9.056443149876209E-5	0.0	0.0	2.1134794967787363	0.0
15	1.1320553937345262E-4	0.0	0.0	2.156293831769776	0.0
16	1.1320553937345262E-4	0.0	0.0	2.2484204997118917	0.0
17	1.1320553937345262E-4	0.0	0.0	2.353995985731574	0.0
18	1.1320553937345262E-4	0.0	0.0	2.505487638521128	0.0
19	1.1320553937345262E-4	0.0	0.0	2.57703353940515	0.0
20	1.1320553937345262E-4	0.0	0.0	2.6554623370830783	0.0
21	1.5848775512283366E-4	0.0	0.0	2.770343318439258	0.0
22	1.8112886299752418E-4	0.0	0.0	2.89382792078782	0.0
23	1.8112886299752418E-4	0.0	0.0	3.0352216394652625	0.0
24	2.0376997087221473E-4	0.0	0.0	3.1429933129487893	0.0
25	2.0376997087221473E-4	0.0	0.0	3.2430217275391717	0.0
26	2.0376997087221473E-4	0.0	0.0	3.333155977988315	0.0
27	2.2641107874690525E-4	0.0	0.0	3.4195318045302594	0.0
28	2.716932944962863E-4	0.0	0.0	3.5117037546881242	0.0
29	2.716932944962863E-4	0.0	0.0	3.6131812001824875	0.0
30	2.943344023709768E-4	0.0	0.0	3.7458128101124246	0.0
31	3.169755102456673E-4	0.0	0.0	3.861486230244218	0.0
32	3.3961661812035785E-4	0.0	0.0	3.9644127066425616	0.0
33	3.3961661812035785E-4	0.0	0.0	4.068471238434639	0.0
34	3.6225772599504836E-4	0.0	0.0	4.177895712793019	0.0
35	3.8489883386973894E-4	0.0	0.0	4.319651689196456	0.0
36	3.8489883386973894E-4	0.0	0.0	4.440691051894551	0.0
37	3.8489883386973894E-4	0.0	0.0	4.566326559491209	0.0
38	3.8489883386973894E-4	0.0	0.0	4.687297998865681	0.0
39	3.8489883386973894E-4	0.0	0.0	4.816556083722289	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGCTA	25	3.8926126E-5	45.000004	20
GTCGAAT	20	7.035469E-4	45.0	30
TAATGCG	195	0.0	43.846153	1
CGTTTTT	15800	0.0	40.713608	1
GCGATCT	310	0.0	39.193546	9
GTCGACG	75	0.0	39.0	1
TAACGCG	65	9.094947E-12	38.07692	1
GGATGCC	4505	0.0	38.057713	8
TACGGGC	680	0.0	38.05147	4
AGGGTAT	4840	0.0	37.84091	6
ACGGGTA	565	0.0	37.831856	5
GATGCCA	4445	0.0	37.559055	9
GCACCGA	1890	0.0	37.5	9
TACGGGA	665	0.0	37.21805	4
CGTAAGG	790	0.0	37.02532	2
TAGGGTC	1430	0.0	36.81818	5
CGTTGTA	80	0.0	36.5625	30
GGGCGAT	7265	0.0	36.48314	7
GTAGGGT	2620	0.0	36.412212	4
AGGGCGA	4165	0.0	36.30252	6
>>END_MODULE