##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549022_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	942254
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.40981837169171	31.0	31.0	33.0	30.0	34.0
2	31.762759298448188	31.0	31.0	34.0	30.0	34.0
3	31.849944919310506	31.0	31.0	34.0	30.0	34.0
4	35.53590751538333	37.0	35.0	37.0	33.0	37.0
5	35.407753111156865	37.0	35.0	37.0	33.0	37.0
6	35.47210094093525	37.0	35.0	37.0	33.0	37.0
7	35.8975159564194	37.0	35.0	37.0	35.0	37.0
8	35.93289282932203	37.0	35.0	37.0	35.0	37.0
9	37.630736510537496	39.0	37.0	39.0	35.0	39.0
10	37.04161086076578	39.0	37.0	39.0	33.0	39.0
11	36.65083512513611	39.0	35.0	39.0	32.0	39.0
12	35.59073986419798	37.0	35.0	39.0	31.0	39.0
13	35.092381672033234	37.0	35.0	39.0	30.0	39.0
14	36.01584392318844	38.0	35.0	40.0	30.0	41.0
15	36.32390629278305	38.0	35.0	40.0	31.0	41.0
16	36.54849435502529	38.0	35.0	40.0	32.0	41.0
17	36.53179079101813	37.0	35.0	40.0	32.0	41.0
18	36.472076531381134	37.0	35.0	40.0	31.0	41.0
19	36.403239466216114	37.0	35.0	40.0	31.0	41.0
20	36.23033385902315	36.0	35.0	40.0	31.0	41.0
21	36.007141386505126	36.0	34.0	40.0	31.0	41.0
22	35.92644021675684	36.0	34.0	40.0	30.0	41.0
23	35.89451358126365	36.0	34.0	40.0	31.0	41.0
24	35.821773110010675	36.0	34.0	40.0	31.0	41.0
25	35.71534957665343	35.0	34.0	40.0	30.0	41.0
26	35.621659340262816	35.0	34.0	40.0	30.0	41.0
27	35.57896278498154	35.0	34.0	40.0	30.0	41.0
28	35.57019126477574	36.0	34.0	40.0	30.0	41.0
29	35.60479127708665	36.0	34.0	40.0	30.0	41.0
30	35.54727706117459	36.0	34.0	40.0	30.0	41.0
31	35.34875415758383	36.0	34.0	40.0	30.0	41.0
32	35.13363700233695	35.0	34.0	40.0	29.0	41.0
33	34.984142280107065	35.0	34.0	40.0	28.0	41.0
34	34.828155677768414	35.0	34.0	40.0	27.0	41.0
35	34.684473613272004	35.0	34.0	40.0	27.0	41.0
36	34.48601438677893	35.0	33.0	40.0	25.0	41.0
37	34.37004884033392	35.0	33.0	40.0	25.0	41.0
38	34.37230725473174	35.0	33.0	40.0	26.0	41.0
39	34.330742029219294	35.0	33.0	40.0	25.0	41.0
40	34.09814126551864	35.0	33.0	40.0	23.0	41.0
41	34.13763592407143	35.0	33.0	40.0	24.0	41.0
42	34.069464284577194	35.0	33.0	40.0	24.0	41.0
43	33.98116431450543	35.0	33.0	40.0	24.0	41.0
44	33.92081540646153	35.0	33.0	39.0	24.0	41.0
45	33.87355744841624	35.0	33.0	39.0	23.0	41.0
46	33.87698752141142	35.0	33.0	39.0	24.0	41.0
47	33.823239805827306	35.0	33.0	39.0	24.0	41.0
48	33.81655052671572	35.0	33.0	39.0	24.0	41.0
49	33.79003219938573	35.0	33.0	39.0	24.0	41.0
50	33.63888187261609	35.0	33.0	39.0	24.0	41.0
51	33.45456002309356	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	3.0
15	25.0
16	73.0
17	207.0
18	516.0
19	1073.0
20	1800.0
21	2802.0
22	4273.0
23	6092.0
24	8553.0
25	11872.0
26	15508.0
27	18562.0
28	20405.0
29	23047.0
30	27542.0
31	33822.0
32	42710.0
33	55356.0
34	99988.0
35	162831.0
36	63056.0
37	73498.0
38	101644.0
39	166878.0
40	113.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	30.430329826140294	25.040169635788228	30.13858259025698	14.390917947814497
2	30.104303086004407	26.084898551770543	30.665298316589794	13.145500045635252
3	28.22986158721534	25.27110524338448	32.567651609863155	13.931381559537027
4	25.72660874880871	28.201100764761943	31.23085707250911	14.841433413920239
5	23.03519008674943	32.34796562285753	30.30966172603141	14.307182564361625
6	22.500196337717856	39.9448556334067	26.870143294695488	10.684804734179956
7	86.56710398682308	4.776206840193833	6.8094165692053314	1.8472726037777498
8	88.13610767372704	3.788150541149202	6.285884697756656	1.7898570873671007
9	84.13092435797567	4.981034837740142	8.245441250448394	2.6425995538358023
10	55.90074438527191	20.20208988234595	13.368051502036607	10.529114230345533
11	50.69864389007635	18.87176918325632	18.10371725670573	12.325869669961603
12	44.40564858307845	21.00378454217228	21.85726990811395	12.733296966635324
13	21.06120005858293	43.58654884988549	21.91362413956322	13.438626951968367
14	15.21298927889932	43.93698514413311	26.4323632481263	14.417662328841269
15	14.094713315093383	21.588340298900295	50.27115830763255	14.045788078373771
16	15.091472150821328	16.484090277143952	48.54773765884783	19.87669991318689
17	16.20168234892078	17.19186121788817	28.818556355292735	37.787900077898314
18	21.503012988005356	21.953846839599514	34.862468081854786	21.68067209054034
19	30.11502206411435	22.205265246950397	26.388638307717454	21.291074381217804
20	32.58017477240744	21.585899343489125	25.28108132202145	20.552844562081987
21	22.489052845623366	27.669821513095194	28.1286150018997	21.712510639381737
22	24.17670819120959	24.241340445357622	23.881777100442132	27.700174262990657
23	19.98155486737122	29.216644344306314	24.773999367474165	26.027801420848306
24	19.908007819547596	21.96658225913607	40.87305546062951	17.25235446068682
25	16.68849376070571	23.671642678088922	37.75913925544492	21.88072430576044
26	16.92017226777493	34.31781663967465	27.408533155603475	21.353477936946938
27	17.979122402239735	34.80229322454455	28.23240867112265	18.986175702093067
28	15.35520146372422	28.584224635820064	38.66218662908303	17.398387271372687
29	15.596537663941994	24.055827834108428	37.721994281796626	22.625640220152953
30	18.18734651166246	31.814776058260296	31.423161907511137	18.574715522566102
31	30.06885616829432	26.926073012160202	25.059485022085344	17.945585797460133
32	30.75805462221439	24.910586742003748	27.609752784281095	16.72160585150076
33	29.272998575755583	26.45985052862604	24.634440395052714	19.632710500565665
34	19.479779337630827	26.966507969188775	28.040209964616757	25.513502728563637
35	20.84448566946917	25.131546270963028	31.467948132881368	22.556019926686435
36	32.4361584031482	24.796074094670864	24.50209816036865	18.265669341812295
37	20.952842864026046	33.44862425630456	28.12702307445763	17.471509805211756
38	21.162871157883117	33.12641814202964	23.91234210732987	21.798368592757367
39	21.788498642616535	31.857121328219357	26.19240671835832	20.16197331080579
40	24.51769904930093	25.998085441929668	25.311646328909188	24.172569179860208
41	17.241529354080747	24.898169707955603	27.67555245188665	30.184748486077
42	22.195501425305704	24.628921713253536	24.899761635397674	28.275815226043083
43	22.313728570003416	25.17155671400705	26.757753217285362	25.75696149870417
44	19.313263727190332	30.77439841061964	28.352546128750845	21.55979173343918
45	16.935879285203352	38.260702528193036	22.857637112710584	21.945781073893027
46	21.43339269453884	33.318404591543256	25.93504511522371	19.313157598694193
47	22.23625476782269	27.445465872259494	26.728567880847415	23.5897114790704
48	23.379895442205605	23.470423049411306	31.520800123958082	21.628881384425007
49	21.78871089960881	24.470365740023393	32.11013166301231	21.63079169735549
50	19.214882611270422	34.38170599434972	26.364334882101854	20.039076512278005
51	19.236745081474847	33.60887828547292	24.166944369564895	22.98743226348734
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	579.0
1	1138.5
2	1698.0
3	4078.5
4	6459.0
5	4871.0
6	3283.0
7	3513.5
8	3744.0
9	4017.0
10	4290.0
11	4418.5
12	4547.0
13	4501.5
14	4456.0
15	4281.0
16	4106.0
17	4041.0
18	3976.0
19	3566.0
20	3156.0
21	3233.0
22	3310.0
23	3540.0
24	3770.0
25	4081.0
26	5573.0
27	6754.0
28	9075.5
29	11397.0
30	12306.5
31	13216.0
32	14950.5
33	16685.0
34	18778.0
35	20871.0
36	24095.5
37	27320.0
38	32713.5
39	38107.0
40	54055.5
41	70004.0
42	84397.5
43	98791.0
44	98448.0
45	98105.0
46	93850.0
47	89595.0
48	82676.0
49	75757.0
50	70188.0
51	64619.0
52	58143.0
53	51667.0
54	45918.5
55	40170.0
56	38132.5
57	36095.0
58	32422.0
59	28749.0
60	27506.0
61	26263.0
62	23860.0
63	21457.0
64	19387.5
65	17318.0
66	14695.5
67	12073.0
68	10236.0
69	8399.0
70	7643.5
71	6888.0
72	5757.5
73	4627.0
74	3478.0
75	2047.5
76	1766.0
77	1322.5
78	879.0
79	587.5
80	296.0
81	236.5
82	177.0
83	129.5
84	82.0
85	54.0
86	26.0
87	14.0
88	2.0
89	1.5
90	1.0
91	1.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	942254.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.714740029191592
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.86016803646581	14.147021154535476
2	5.927873104350964	2.1002146193922853
3	1.9863002116537434	1.055603756081229
4	0.9777041771276233	0.6927910129308213
5	0.6173922228883951	0.5468471362256316
6	0.48026996619795775	0.5104714557015274
7	0.38600181019215374	0.4786545202845953
8	0.33905895319044976	0.48050729682709276
9	0.28684877910646944	0.45733063946059
>10	5.764461129780114	26.466063747090686
>50	2.426244802518192	30.23908508041947
>100	0.9434729170188316	21.753136307248987
>500	0.0024022225767506853	0.2989471077867368
>1k	0.001801666932563014	0.7733261660148718
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3414	0.3623226858150775	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2836	0.3009804150473227	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1019	0.10814493756460572	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.021437956219872774	0.0
2	0.0	0.0	0.0	0.0857518248794911	0.0
3	0.0	0.0	0.0	0.12236615604709558	0.0
4	0.0	0.0	0.0	0.1872106671874038	0.0
5	0.0	0.0	0.0	0.3080910242885676	0.0
6	0.0	0.0	0.0	0.4496664381366383	0.0
7	0.0	0.0	0.0	0.5222583294950194	0.0
8	0.0	0.0	0.0	0.7295272824525022	0.0
9	0.0	0.0	0.0	0.8189936046968227	0.0
10	0.0	0.0	0.0	0.9612057895217213	0.0
11	0.0	0.0	0.0	1.1049037732925517	0.0
12	0.0	0.0	0.0	1.2350173095577202	0.0
13	0.0	0.0	0.0	1.2844731887580207	0.0
14	0.0	0.0	0.0	1.3037885750551337	0.0
15	0.0	0.0	0.0	1.3297900566089398	0.0
16	0.0	0.0	0.0	1.3928303833149023	0.0
17	0.0	0.0	0.0	1.4692429005342509	0.0
18	0.0	0.0	0.0	1.5806778214791342	0.0
19	0.0	0.0	0.0	1.626313074818467	0.0
20	1.0612849613798402E-4	0.0	0.0	1.680756993337253	0.0
21	1.0612849613798402E-4	0.0	0.0	1.7511201862767365	0.0
22	1.0612849613798402E-4	0.0	0.0	1.8302920443956725	0.0
23	1.0612849613798402E-4	0.0	0.0	1.9163622547635775	0.0
24	1.0612849613798402E-4	0.0	0.0	1.9870438331914748	0.0
25	1.0612849613798402E-4	0.0	0.0	2.04679417651716	0.0
26	1.0612849613798402E-4	0.0	0.0	2.105058720896913	0.0
27	1.0612849613798402E-4	0.0	0.0	2.158016840469767	0.0
28	2.1225699227596804E-4	0.0	0.0	2.2073665911739297	0.0
29	2.1225699227596804E-4	0.0	0.0	2.270300789383754	0.0
30	2.1225699227596804E-4	0.0	0.0	2.3559464857671073	0.0
31	2.1225699227596804E-4	0.0	0.0	2.4291751481023165	0.0
32	2.1225699227596804E-4	0.0	0.0	2.496248357661522	0.0
33	2.1225699227596804E-4	0.0	0.0	2.5606683548172784	0.0
34	2.1225699227596804E-4	0.0	0.0	2.6334725031679356	0.0
35	2.1225699227596804E-4	0.0	0.0	2.738964228329092	0.0
36	3.183854884139521E-4	0.0	0.0	2.812829661641129	0.0
37	3.183854884139521E-4	0.0	0.0	2.887756379914545	0.0
38	3.183854884139521E-4	0.0	0.0	2.9657608245759635	0.0
39	3.183854884139521E-4	0.0	0.0	3.043128498260554	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	60	0.0	45.000004	1
TCGCACC	20	7.0326374E-4	45.000004	45
CACGGTT	20	7.0326374E-4	45.000004	25
CAACGCG	20	7.0326374E-4	45.000004	41
ATTACGC	20	7.0326374E-4	45.000004	23
ATCAAGT	30	2.165083E-6	45.000004	37
CGTTAGA	20	7.0326374E-4	45.000004	16
GAACGTA	20	7.0326374E-4	45.000004	11
CATACGT	20	7.0326374E-4	45.000004	23
GTATACG	55	1.8189894E-12	45.000004	1
CACAACG	30	2.165083E-6	45.000004	12
ATTGACG	20	7.0326374E-4	45.000004	1
GCGTATG	20	7.0326374E-4	45.000004	1
AATCGAT	20	7.0326374E-4	45.000004	16
CGCTTAA	20	7.0326374E-4	45.000004	27
CGTTGTA	25	3.890269E-5	45.0	13
TGATTCG	35	1.2116107E-7	45.0	15
ACTAAGC	25	3.890269E-5	45.0	24
CCTATGC	140	0.0	45.0	35
TATCGCG	25	3.890269E-5	45.0	1
>>END_MODULE