##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549021_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3216683
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252118408932432	31.0	31.0	33.0	30.0	34.0
2	31.609899079268924	31.0	31.0	34.0	30.0	34.0
3	31.669979292333128	31.0	31.0	34.0	30.0	34.0
4	35.422229980386625	37.0	35.0	37.0	33.0	37.0
5	35.292578721621	37.0	35.0	37.0	33.0	37.0
6	35.364566542615485	37.0	35.0	37.0	33.0	37.0
7	35.823043178329975	37.0	35.0	37.0	35.0	37.0
8	35.8476122763729	37.0	35.0	37.0	35.0	37.0
9	37.52140357007514	39.0	37.0	39.0	35.0	39.0
10	36.831214328549	39.0	37.0	39.0	32.0	39.0
11	36.52906550008192	39.0	35.0	39.0	32.0	39.0
12	36.04932254748137	38.0	35.0	39.0	31.0	39.0
13	35.854737007034885	38.0	35.0	39.0	31.0	39.0
14	36.92314816225286	39.0	35.0	41.0	31.0	41.0
15	37.02403811628314	39.0	35.0	41.0	31.0	41.0
16	37.1020877717823	39.0	35.0	41.0	32.0	41.0
17	37.011443154330095	39.0	35.0	41.0	31.0	41.0
18	36.89720249088891	38.0	35.0	40.0	31.0	41.0
19	36.8044488686016	38.0	35.0	40.0	31.0	41.0
20	36.65672184669736	38.0	35.0	40.0	31.0	41.0
21	36.48577587533493	38.0	35.0	40.0	31.0	41.0
22	36.41374701827939	38.0	35.0	40.0	30.0	41.0
23	36.33356752903534	38.0	35.0	40.0	30.0	41.0
24	36.26523129571674	38.0	35.0	40.0	30.0	41.0
25	36.13184389011911	38.0	35.0	40.0	30.0	41.0
26	36.04731924159142	38.0	35.0	40.0	30.0	41.0
27	35.98678234690829	38.0	35.0	40.0	30.0	41.0
28	35.940088594368795	38.0	35.0	40.0	30.0	41.0
29	35.88451084548897	38.0	35.0	40.0	30.0	41.0
30	35.740489815129436	38.0	34.0	40.0	29.0	41.0
31	35.55276817765381	37.0	34.0	40.0	29.0	41.0
32	35.326299483038895	37.0	34.0	40.0	27.0	41.0
33	35.06469770257125	37.0	34.0	40.0	26.0	41.0
34	34.806754349123	37.0	34.0	40.0	24.0	41.0
35	34.61896214205752	37.0	33.0	40.0	24.0	41.0
36	34.48051642017569	37.0	33.0	40.0	23.0	41.0
37	34.430948899844964	37.0	33.0	40.0	23.0	41.0
38	34.36639109293642	37.0	33.0	40.0	23.0	41.0
39	34.271863904525254	37.0	33.0	40.0	23.0	41.0
40	34.124596362153184	37.0	33.0	40.0	23.0	41.0
41	34.042972527911516	36.0	33.0	40.0	23.0	41.0
42	33.93298624701284	36.0	33.0	40.0	22.0	41.0
43	33.833626440653305	36.0	33.0	40.0	22.0	41.0
44	33.72134151857674	36.0	33.0	40.0	22.0	41.0
45	33.66356927306794	36.0	33.0	40.0	22.0	41.0
46	33.607387796683724	35.0	33.0	40.0	22.0	41.0
47	33.53529085707233	35.0	32.0	40.0	22.0	41.0
48	33.45643820046924	35.0	32.0	40.0	21.0	41.0
49	33.39603156419206	35.0	32.0	40.0	21.0	41.0
50	33.271268570760625	35.0	32.0	39.0	20.0	41.0
51	33.11737525892356	35.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	8.0
13	9.0
14	45.0
15	156.0
16	409.0
17	1078.0
18	2274.0
19	4441.0
20	7432.0
21	11493.0
22	16887.0
23	23910.0
24	33579.0
25	47490.0
26	62587.0
27	72838.0
28	78127.0
29	84890.0
30	97475.0
31	113501.0
32	136695.0
33	169473.0
34	261356.0
35	360978.0
36	252111.0
37	306224.0
38	421331.0
39	649553.0
40	331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.132199535981634	23.987816020416062	26.194654555640078	17.685329887962226
2	33.31680491985067	25.077012562319634	28.535233344410997	13.070949173418706
3	27.529694408805593	25.090597985564635	33.80389052946778	13.575817076161997
4	24.818516465564063	27.300514225368183	33.46509432231898	14.415874986748772
5	21.49605665214757	31.505808934234423	32.334581928029586	14.663552485588415
6	20.43020714195337	40.07790012257969	29.234960361341173	10.256932374125768
7	84.4751254630935	4.238807492065584	9.542252065248581	1.7438149795923317
8	85.69958556687122	3.2698279563140047	9.300792151418092	1.7297943253966896
9	81.12515283601151	5.017249135211645	11.01986114267399	2.8377368861028582
10	42.42892445416599	23.785122749117647	18.55134621596222	15.23460658075415
11	35.24400756928799	23.959588184474505	24.95996652452231	15.836437721715196
12	30.829428949013625	22.34833211727733	30.524798371490135	16.297440562218906
13	22.065369823510743	29.83110241201884	29.71837759580288	18.38515016866754
14	18.804153222434415	31.742605659308047	31.41344670892345	18.039794409334085
15	18.300870803868456	24.23384585922828	39.84589093796311	17.61939239894015
16	20.452683711761463	21.69996235252277	39.18962484024692	18.65772909546884
17	19.61958949638494	22.240954424169246	31.568202399801287	26.57125367964453
18	22.28957593894083	24.066686086257178	33.0509098969342	20.592828077867793
19	26.117587589451617	25.993080449643312	28.21997069652185	19.669361264383216
20	27.663310310652307	24.38791761575511	28.558487112345233	19.390284961247346
21	23.96397158190596	26.655533044443608	29.867288756772115	19.513206616878318
22	23.08946825036847	24.075732672445497	29.537881102987146	23.296917974198887
23	21.471776982686823	27.889350613660095	28.978298452163298	21.66057395148978
24	21.651092134350822	23.83365100011409	35.726709781473645	18.788547084061438
25	20.555273864412502	24.928567720226084	33.04130372809506	21.474854687266355
26	19.591548187993656	28.990889061806836	29.844998714514297	21.572564035685208
27	19.367870567289348	28.99536572301343	30.864060897514612	20.77270281218261
28	18.196353199864582	27.834915656904958	34.908662121819276	19.060069021411184
29	19.763277885946486	25.540688964377278	33.9932781688466	20.70275498082963
30	20.779790859093048	27.384389447141665	32.257390610140945	19.578429083624343
31	26.048261516599553	26.18974266348285	28.211701308459673	19.55029451145792
32	26.812029659124008	26.054043870658067	28.43839445789343	18.6955320123245
33	24.907521194970098	26.840195319215475	27.87219007903483	20.380093406779594
34	20.756226211908356	27.569424777014085	29.732025194897975	21.942323816179588
35	21.115726977137626	28.045038942289306	30.394042558747632	20.445191521825436
36	25.24812050177154	28.542570094721796	26.748112885229908	19.461196518276747
37	21.402264382284482	31.89574477808351	27.672947567416493	19.02904327221551
38	21.350440811233188	31.62854406231512	26.18576340907699	20.8352517173747
39	21.97962932623451	30.48242552965275	27.06691955657427	20.471025587538467
40	23.338824497160584	27.409943721529288	27.337073625222008	21.91415815608812
41	18.92505416293741	27.605797649317637	28.269462673194717	25.199685514550236
42	21.53668857018239	28.112779530964037	26.16931789672778	24.18121400212579
43	22.05797089734985	26.49894316598807	27.167333554472105	24.275752382189978
44	20.988235396524928	28.855687675782786	28.613730355151564	21.54234657254072
45	20.642848549266436	31.272804936016385	25.9516402455573	22.132706269159875
46	21.30029598813436	30.67038312447947	27.169198829974857	20.860122057411314
47	22.065494175210922	28.311089404831	27.622554040917308	22.00086237904077
48	21.89245878440617	26.8245891808425	30.30447824669077	20.97847378806056
49	21.66343404059399	26.696848896829433	29.54098989549172	22.09872716708485
50	20.282508410060924	30.08204414298829	28.24819853246341	21.38724891448738
51	19.91262427786636	30.805553422578473	27.30166447859488	21.980157820960287
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1987.0
1	4047.5
2	6108.0
3	31981.5
4	57855.0
5	40317.5
6	22780.0
7	22603.0
8	22426.0
9	22552.5
10	22679.0
11	22714.5
12	22750.0
13	22312.0
14	21874.0
15	21139.5
16	20405.0
17	19259.0
18	18113.0
19	18270.0
20	18427.0
21	18620.5
22	18814.0
23	21472.0
24	24130.0
25	26757.5
26	33423.0
27	37461.0
28	43321.0
29	49181.0
30	55217.5
31	61254.0
32	70058.0
33	78862.0
34	89017.5
35	99173.0
36	103679.0
37	108185.0
38	118680.5
39	129176.0
40	151527.5
41	173879.0
42	197663.5
43	221448.0
44	234033.5
45	246619.0
46	240224.0
47	233829.0
48	221325.0
49	208821.0
50	201809.5
51	194798.0
52	184077.5
53	173357.0
54	166755.5
55	160154.0
56	156133.0
57	152112.0
58	144202.5
59	136293.0
60	126673.5
61	117054.0
62	106492.5
63	95931.0
64	84196.0
65	72461.0
66	62350.0
67	52239.0
68	44086.5
69	35934.0
70	30506.5
71	25079.0
72	21848.5
73	18618.0
74	14847.5
75	8860.0
76	6643.0
77	5584.0
78	4525.0
79	3627.0
80	2729.0
81	1966.5
82	1204.0
83	842.5
84	481.0
85	361.0
86	241.0
87	163.5
88	86.0
89	55.5
90	25.0
91	20.5
92	16.0
93	9.0
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3216683.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.25964617536664
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.06802507228116	15.228221866771532
2	6.204119527408337	2.389782938551349
3	2.3220709029055024	1.3416679195222236
4	1.4104781741352623	1.086612422876893
5	0.9408987613721874	0.906068861543453
6	0.7489692897262956	0.8654930109806469
7	0.5677239489540903	0.7653913667496383
8	0.46476960655164573	0.7161038540199249
9	0.40404271947986337	0.7003547835223578
>10	5.174363998402735	24.416065539410727
>50	1.7992704412457858	24.569629798090865
>100	0.8906867226551913	25.061615493570944
>500	0.0032720249157242526	0.4206769403979118
>1k	0.0011452087205034885	0.433091494702217
>5k	0.0	0.0
>10k+	1.6360124578621263E-4	1.0992237092893082
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	34886	1.0845333531467043	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4449	0.13831017852862718	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0147356764716946	0.0
2	0.0	0.0	0.0	0.051605955575976865	0.0
3	3.10879250457692E-5	0.0	0.0	0.08204103419578491	0.0
4	3.10879250457692E-5	0.0	0.0	0.1227351280806968	0.0
5	3.10879250457692E-5	0.0	0.0	0.21948075082313054	0.0
6	3.10879250457692E-5	0.0	0.0	0.3753556070026173	0.0
7	3.10879250457692E-5	0.0	0.0	0.4682152391143299	0.0
8	3.10879250457692E-5	0.0	0.0	0.6994161376797154	0.0
9	3.10879250457692E-5	0.0	0.0	0.8316641708244176	0.0
10	3.10879250457692E-5	0.0	0.0	1.0170103799472936	0.0
11	3.10879250457692E-5	0.0	0.0	1.1651754307154296	0.0
12	6.21758500915384E-5	0.0	0.0	1.2869468331197074	0.0
13	9.326377513730759E-5	0.0	0.0	1.337122744143579	0.0
14	9.326377513730759E-5	0.0	0.0	1.3630500736317506	0.0
15	9.326377513730759E-5	0.0	0.0	1.3956613070047623	0.0
16	1.243517001830768E-4	0.0	0.0	1.4523967702132912	0.0
17	1.243517001830768E-4	0.0	0.0	1.5169042146832623	0.0
18	1.243517001830768E-4	0.0	0.0	1.5997535349302372	0.0
19	1.55439625228846E-4	0.0	0.0	1.6472247964751268	0.0
20	1.55439625228846E-4	0.0	0.0	1.6991416313015613	0.0
21	1.55439625228846E-4	0.0	0.0	1.7653899995740954	0.0
22	1.55439625228846E-4	0.0	0.0	1.842083910662008	0.0
23	1.55439625228846E-4	0.0	0.0	1.928570518139338	0.0
24	1.55439625228846E-4	0.0	0.0	1.9988292287427762	0.0
25	1.55439625228846E-4	0.0	0.0	2.061657925260276	0.0
26	1.55439625228846E-4	0.0	0.0	2.1229944013755784	0.0
27	1.8652755027461518E-4	0.0	0.0	2.185854185818124	0.0
28	1.8652755027461518E-4	0.0	0.0	2.2544963243191822	0.0
29	1.8652755027461518E-4	0.0	0.0	2.325407881348582	0.0
30	1.8652755027461518E-4	0.0	0.0	2.4148478417052597	0.0
31	1.8652755027461518E-4	0.0	0.0	2.491075433917486	0.0
32	1.8652755027461518E-4	0.0	0.0	2.569385917107778	0.0
33	1.8652755027461518E-4	0.0	0.0	2.652670468305394	0.0
34	1.8652755027461518E-4	0.0	0.0	2.7354576127022776	0.0
35	1.8652755027461518E-4	0.0	0.0	2.8383586446037734	0.0
36	2.176154753203844E-4	0.0	0.0	2.930534342364479	0.0
37	2.7979132541192277E-4	0.0	0.0	3.025507953379304	0.0
38	2.7979132541192277E-4	0.0	0.0	3.1312690743850107	0.0
39	2.7979132541192277E-4	0.0	0.0	3.2369058436905345	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTCGC	695	0.0	42.410072	19
CGTTTTT	20785	0.0	41.925667	1
GTACCGA	640	0.0	39.375004	42
TATTACG	110	0.0	38.863636	1
ATAGGGC	1885	0.0	38.67374	4
TCTACGG	205	0.0	38.414635	2
TACCGAC	665	0.0	38.23308	43
GGGCGAT	5745	0.0	38.146214	7
TATAGCG	90	0.0	37.5	1
CGTTAGG	580	0.0	37.241383	2
CACCTCG	800	0.0	37.125	18
AGGGCGA	2905	0.0	37.099827	6
CCGACCA	720	0.0	36.875	45
TGTACCG	705	0.0	36.70213	41
GTAGGGC	1935	0.0	36.16279	4
CTCGCGG	820	0.0	35.94512	21
AGGGCTT	2425	0.0	35.907215	6
TGGGCGA	2100	0.0	35.892857	6
GGCGATA	1185	0.0	35.886078	8
GGTACCT	1430	0.0	35.874126	8
>>END_MODULE