##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549019_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1620238
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.324545529730816	31.0	31.0	33.0	30.0	34.0
2	31.670758246627965	31.0	31.0	34.0	30.0	34.0
3	31.782151140758334	31.0	31.0	34.0	30.0	34.0
4	35.47494010139251	37.0	35.0	37.0	33.0	37.0
5	35.328509762146055	37.0	35.0	37.0	33.0	37.0
6	35.40122685679511	37.0	35.0	37.0	33.0	37.0
7	35.838278080133904	37.0	35.0	37.0	35.0	37.0
8	35.87178179995779	37.0	35.0	37.0	35.0	37.0
9	37.532325497858956	39.0	37.0	39.0	35.0	39.0
10	36.950151150633424	39.0	37.0	39.0	32.0	39.0
11	36.58810495741984	39.0	35.0	39.0	32.0	39.0
12	35.59060150422346	37.0	35.0	39.0	31.0	39.0
13	35.14874975157971	37.0	35.0	39.0	30.0	39.0
14	36.0785940090283	38.0	35.0	40.0	30.0	41.0
15	36.35817762575621	38.0	35.0	40.0	31.0	41.0
16	36.58191265727628	38.0	35.0	40.0	32.0	41.0
17	36.55580167851883	38.0	35.0	40.0	32.0	41.0
18	36.50668543757152	38.0	35.0	40.0	31.0	41.0
19	36.43225007684056	37.0	35.0	40.0	31.0	41.0
20	36.26599734113137	37.0	35.0	40.0	31.0	41.0
21	36.05295086277448	37.0	34.0	40.0	31.0	41.0
22	35.975692459996615	37.0	34.0	40.0	30.0	41.0
23	35.95709087183488	36.0	34.0	40.0	30.0	41.0
24	35.87531029391978	36.0	34.0	40.0	30.0	41.0
25	35.759626054937605	36.0	34.0	40.0	30.0	41.0
26	35.656780670494086	36.0	34.0	40.0	30.0	41.0
27	35.592021048759506	36.0	34.0	40.0	30.0	41.0
28	35.593514039295464	36.0	34.0	40.0	30.0	41.0
29	35.63623986105745	36.0	34.0	40.0	30.0	41.0
30	35.57374780742089	36.0	34.0	40.0	30.0	41.0
31	35.37350438639262	36.0	34.0	40.0	29.0	41.0
32	35.16491774665204	36.0	34.0	40.0	29.0	41.0
33	34.99735470961674	36.0	34.0	40.0	28.0	41.0
34	34.84148686797865	35.0	34.0	40.0	27.0	41.0
35	34.70629500110478	35.0	34.0	40.0	27.0	41.0
36	34.517959707154134	35.0	33.0	40.0	25.0	41.0
37	34.43504843115641	35.0	33.0	40.0	25.0	41.0
38	34.434146711779384	35.0	33.0	40.0	26.0	41.0
39	34.38623399772132	35.0	33.0	40.0	25.0	41.0
40	34.19589776316813	35.0	33.0	40.0	24.0	41.0
41	34.1928963522643	35.0	33.0	40.0	24.0	41.0
42	34.110883092483945	35.0	33.0	40.0	24.0	41.0
43	34.02348543855903	35.0	33.0	40.0	24.0	41.0
44	33.935303331979625	35.0	33.0	40.0	23.0	41.0
45	33.882954849843046	35.0	33.0	39.0	23.0	41.0
46	33.87616263783469	35.0	33.0	39.0	23.0	41.0
47	33.82856716112077	35.0	33.0	39.0	23.0	41.0
48	33.80336037051347	35.0	33.0	39.0	23.0	41.0
49	33.75755166833515	35.0	33.0	39.0	24.0	41.0
50	33.60450193119776	35.0	33.0	39.0	24.0	41.0
51	33.433881318670466	35.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	1.0
13	5.0
14	13.0
15	45.0
16	148.0
17	384.0
18	884.0
19	1704.0
20	2932.0
21	4793.0
22	7154.0
23	10389.0
24	14722.0
25	20443.0
26	27168.0
27	32151.0
28	36366.0
29	41003.0
30	48999.0
31	59587.0
32	74053.0
33	96147.0
34	165764.0
35	262994.0
36	111791.0
37	131793.0
38	179625.0
39	288988.0
40	188.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.3229673665227	24.91029095725443	28.722014913858334	14.044726762364542
2	28.444277939413837	27.053803206689388	30.54322883428237	13.958690019614403
3	28.249985495958	25.41083470453106	32.07109079036536	14.268089009145571
4	25.459222657412063	29.258973064451027	30.548660135115952	14.733144143020963
5	23.053094668807915	33.292824881282876	29.014996562233446	14.63908388767576
6	21.574176139554808	41.29257553519915	26.48493616369941	10.648312161546638
7	85.6658713102643	5.576032656930648	6.706545581575051	2.0515504512300047
8	87.24125714864113	4.429843023062044	6.184276630964093	2.144623197332738
9	81.70645300258357	6.320614625752513	8.415306887012894	3.5576254846510205
10	53.76080551128908	21.14065958211078	13.667806828379533	11.430728078220607
11	49.0134165474455	20.016195151576497	18.198746110139375	12.77164219083863
12	42.64071080915273	21.43845533804293	22.900092455552826	13.020741397251514
13	21.648547929378275	41.49569384250956	23.252262939148448	13.603495288963721
14	15.877605635715247	43.29388645371853	26.17566061282355	14.652847297742678
15	14.359927368695216	22.847507588391338	48.555397416922695	14.237167625990748
16	16.100967882496274	17.31097530115946	46.67659936379717	19.911457452547097
17	16.555160414704506	18.350081901547796	27.948486580366588	37.1462711033811
18	21.541773492536283	22.53156635012881	35.263955048579284	20.662705108755627
19	30.54150069310805	23.095989601527677	25.74158858143063	20.620921123933645
20	32.59101440652546	21.361429617130323	25.348436464272535	20.699119512071682
21	22.112430396028238	28.298743764804925	28.081553450789325	21.507272388377512
22	22.688703758336736	25.01873181594309	24.72655251882748	27.56601190689269
23	19.83060513331992	30.45904367136186	24.234587758094797	25.47576343722342
24	20.11414372456392	23.001312152905932	38.91996114151131	17.964582981018836
25	17.11112811821473	24.493500337604722	37.083008792535416	21.31236275164513
26	16.873385268090242	34.842041724734266	26.843093422077498	21.44147958509799
27	18.096600622871456	34.3816772597606	28.577715125802506	18.944006991565438
28	15.14166437276499	29.433885639023405	37.99139385695188	17.43305613125973
29	16.066466778337503	24.39820569570643	36.924390120463784	22.61093740549228
30	18.91543094286148	31.583569821223794	29.90819867204695	19.592800563867776
31	29.43394735835106	26.904010398472323	24.821229967449227	18.84081227572739
32	29.881103887206695	26.130111749014652	26.427537188980878	17.561247174797774
33	28.627028868598316	28.036436622274014	23.932718526537457	19.40381598259021
34	20.15784100854319	27.901147856055715	26.62898907444462	25.31202206095648
35	21.069373758670025	25.77220136794718	30.168037041471685	22.990387831911114
36	30.703390489545363	25.034470244494944	25.1804981737251	19.081641092234598
37	20.85773818414332	33.68943328078961	27.904974454370286	17.547854080696787
38	20.148644828722695	33.42570659372265	23.754719985582366	22.670928591972288
39	21.283107790336974	32.39610476979308	26.47814703765743	19.842640402212513
40	24.424621567942488	26.04734613063019	25.337141827311786	24.19089047411553
41	16.867953967256664	24.97855253364012	27.514229390990707	30.639264108112513
42	21.707489887288165	24.81345333216478	25.388862623886123	28.090194156660935
43	22.633835276052036	24.682855234848216	26.741688566741427	25.94162092235832
44	19.836406750119426	29.884436730900028	28.79200463141835	21.4871518875622
45	16.834316933685052	38.551188158776675	22.31474635207914	22.299748555459136
46	21.97257439956352	33.398858686193016	25.2967156676982	19.331851246545263
47	22.138599390953676	27.64482748830727	26.24132997744776	23.975243143291294
48	22.633711837396728	24.790864058243294	30.935887196819234	21.639536907540744
49	22.007384100360564	24.405797173007915	32.34382849926986	21.24299022736166
50	19.949476558382166	34.593868308236196	25.34973257015327	20.106922563228366
51	19.50435676733912	33.93686606535583	23.179742729154608	23.379034438150445
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	880.0
1	1628.5
2	2377.0
3	6410.5
4	10444.0
5	7874.0
6	5304.0
7	5628.0
8	5952.0
9	6493.0
10	7034.0
11	7416.0
12	7798.0
13	7927.0
14	8056.0
15	7605.0
16	7154.0
17	6654.0
18	6154.0
19	6011.0
20	5868.0
21	6077.0
22	6286.0
23	6332.5
24	6379.0
25	8223.5
26	11683.0
27	13298.0
28	16685.0
29	20072.0
30	23203.5
31	26335.0
32	29374.5
33	32414.0
34	36456.5
35	40499.0
36	41711.0
37	42923.0
38	53751.0
39	64579.0
40	92209.5
41	119840.0
42	139123.5
43	158407.0
44	167615.0
45	176823.0
46	160350.5
47	143878.0
48	135133.0
49	126388.0
50	118247.0
51	110106.0
52	103309.5
53	96513.0
54	86212.5
55	75912.0
56	66901.0
57	57890.0
58	55524.0
59	53158.0
60	49853.0
61	46548.0
62	41934.0
63	37320.0
64	33536.0
65	29752.0
66	24749.5
67	19747.0
68	15947.0
69	12147.0
70	11341.0
71	10535.0
72	8588.0
73	6641.0
74	5342.0
75	3250.0
76	2457.0
77	1851.5
78	1246.0
79	919.5
80	593.0
81	420.5
82	248.0
83	184.5
84	121.0
85	72.0
86	23.0
87	21.0
88	19.0
89	11.0
90	3.0
91	2.5
92	2.0
93	1.5
94	1.0
95	2.0
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1620238.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.835255387757936
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.1275320091077	13.489674649640767
2	7.299866355805647	2.457902287929798
3	2.4627145416530674	1.2438128476762385
4	1.2178744893217968	0.8201291223187089
5	0.7084961039681709	0.5963856425767826
6	0.48086622238676724	0.4857303396736578
7	0.3511159856435538	0.4137789102323504
8	0.28270500832735496	0.3807528811671403
9	0.22404246333001082	0.33946308790768115
>10	3.536572817305768	15.258908676269911
>50	1.8032720122511194	22.004288507620743
>100	1.497188364351253	40.68697661390129
>500	0.005169084365207778	0.5645918540580103
>1k	0.0018461015590027778	0.47986888839896946
>5k	7.384406236011111E-4	0.7777356906279671
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7006	0.43240560954625185	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5506	0.33982661806475345	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1990	0.1228214620321212	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1883	0.11621749397310766	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05283174447210842	0.0
2	0.0	0.0	0.0	0.16516092080299313	0.0
3	0.0	0.0	0.0	0.2358295509672036	0.0
4	0.0	0.0	0.0	0.34309774243043306	0.0
5	0.0	0.0	0.0	0.5436238379793585	0.0
6	0.0	0.0	0.0	0.7819838813803898	0.0
7	0.0	0.0	0.0	0.9273328980063423	0.0
8	0.0	0.0	0.0	1.3161029429009812	0.0
9	0.0	0.0	0.0	1.4941014838560756	0.0
10	0.0	0.0	0.0	1.7599266280632846	0.0
11	0.0	0.0	0.0	1.991312387439376	0.0
12	0.0	0.0	0.0	2.206959718263613	0.0
13	0.0	0.0	0.0	2.2880589148014057	0.0
14	0.0	0.0	0.0	2.3181162273690656	0.0
15	0.0	0.0	0.0	2.365393232352284	0.0
16	0.0	0.0	0.0	2.467600438947858	0.0
17	0.0	0.0	0.0	2.585916390061213	0.0
18	0.0	0.0	0.0	2.772185320921988	0.0
19	0.0	0.0	0.0	2.845816478813606	0.0
20	0.0	0.0	0.0	2.9269773946790534	0.0
21	0.0	0.0	0.0	3.0278267760662323	0.0
22	0.0	0.0	0.0	3.1325644750956343	0.0
23	0.0	0.0	0.0	3.2514976194855323	0.0
24	0.0	0.0	0.0	3.3422250311374007	0.0
25	0.0	0.0	0.0	3.413942889871735	0.0
26	0.0	0.0	0.0	3.488499837678168	0.0
27	6.171932765433226E-5	0.0	0.0	3.563859136744108	0.0
28	6.171932765433226E-5	0.0	0.0	3.6453903685754807	0.0
29	6.171932765433226E-5	0.0	0.0	3.725502055870804	0.0
30	6.171932765433226E-5	0.0	0.0	3.838633583461195	0.0
31	6.171932765433226E-5	0.0	0.0	3.946395529545659	0.0
32	1.2343865530866453E-4	0.0	0.0	4.037987011784688	0.0
33	1.2343865530866453E-4	0.0	0.0	4.125813615036804	0.0
34	1.2343865530866453E-4	0.0	0.0	4.222466082143487	0.0
35	1.2343865530866453E-4	0.0	0.0	4.354236846685487	0.0
36	1.2343865530866453E-4	0.0	0.0	4.45656749193637	0.0
37	1.2343865530866453E-4	0.0	0.0	4.556552802736388	0.0
38	1.2343865530866453E-4	0.0	0.0	4.6572787454682585	0.0
39	1.8515798296299678E-4	0.0	0.0	4.7607203386169195	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTCG	30	2.1660544E-6	45.000004	1
CGATATC	30	2.1660544E-6	45.000004	10
TCGTTAG	100	0.0	45.0	1
ACACGAA	20	7.034144E-4	45.0	42
CGTATGA	45	3.8562575E-10	45.0	15
GTCGAAA	35	1.2123382E-7	45.0	39
CGCATCG	20	7.034144E-4	45.0	45
ATTACCG	20	7.034144E-4	45.0	1
CACGCTA	20	7.034144E-4	45.0	15
CTAAGCG	35	1.2123382E-7	45.0	1
CGCGAAC	20	7.034144E-4	45.0	28
GCGCATC	35	1.2123382E-7	45.0	21
GCGTTAG	50	2.1827873E-11	45.0	1
CGTTAGT	40	6.8175723E-9	45.0	21
GTAGCAC	20	7.034144E-4	45.0	33
CGCTAAG	35	1.2123382E-7	45.0	42
ACGTCTT	35	1.2123382E-7	45.0	34
CTATTAG	50	2.1827873E-11	45.0	1
ACCGTAT	25	3.8915154E-5	45.0	20
CCGCGAA	20	7.034144E-4	45.0	27
>>END_MODULE