##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549018_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6613561
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27256042546519	31.0	31.0	33.0	30.0	34.0
2	31.62409494673142	31.0	31.0	34.0	30.0	34.0
3	31.72138564987909	31.0	31.0	34.0	30.0	34.0
4	35.43628447669871	37.0	35.0	37.0	33.0	37.0
5	35.29619383566584	37.0	35.0	37.0	33.0	37.0
6	35.36427637092937	37.0	35.0	37.0	33.0	37.0
7	35.83231681086785	37.0	35.0	37.0	35.0	37.0
8	35.855152466273466	37.0	35.0	37.0	35.0	37.0
9	37.498346503494865	39.0	37.0	39.0	35.0	39.0
10	36.81306258458945	39.0	37.0	39.0	32.0	39.0
11	36.51697927939275	39.0	35.0	39.0	32.0	39.0
12	35.94112884117951	37.0	35.0	39.0	31.0	39.0
13	35.69142947347125	37.0	35.0	39.0	30.0	39.0
14	36.732305183243945	38.0	35.0	41.0	31.0	41.0
15	36.89514861963169	38.0	35.0	41.0	31.0	41.0
16	37.00782785552292	38.0	35.0	41.0	32.0	41.0
17	36.94616999828081	38.0	35.0	41.0	32.0	41.0
18	36.85689600504176	38.0	35.0	40.0	31.0	41.0
19	36.81315481931746	38.0	35.0	40.0	31.0	41.0
20	36.66884738796542	38.0	35.0	40.0	31.0	41.0
21	36.49689357972203	38.0	35.0	40.0	31.0	41.0
22	36.41440730644202	38.0	35.0	40.0	31.0	41.0
23	36.35779015268779	38.0	35.0	40.0	31.0	41.0
24	36.29021959576694	38.0	35.0	40.0	30.0	41.0
25	36.17920769159005	38.0	35.0	40.0	30.0	41.0
26	36.086269711582005	38.0	35.0	40.0	30.0	41.0
27	36.02686011363621	38.0	34.0	40.0	30.0	41.0
28	35.98119091968759	38.0	35.0	40.0	30.0	41.0
29	35.974309906569246	38.0	35.0	40.0	30.0	41.0
30	35.89737450066613	38.0	35.0	40.0	30.0	41.0
31	35.76722419283651	38.0	34.0	40.0	30.0	41.0
32	35.59327131026689	38.0	34.0	40.0	29.0	41.0
33	35.40915053176345	37.0	34.0	40.0	28.0	41.0
34	35.21111425448408	37.0	34.0	40.0	27.0	41.0
35	35.06290181643445	37.0	34.0	40.0	26.0	41.0
36	34.90706655612612	37.0	34.0	40.0	25.0	41.0
37	34.832679701600995	37.0	34.0	40.0	25.0	41.0
38	34.809977710948765	37.0	33.0	40.0	25.0	41.0
39	34.72727430804675	37.0	34.0	40.0	25.0	41.0
40	34.59202856071033	37.0	33.0	40.0	24.0	41.0
41	34.542357740406416	37.0	33.0	40.0	24.0	41.0
42	34.45728919715113	36.0	33.0	40.0	24.0	41.0
43	34.37700294894082	36.0	33.0	40.0	24.0	41.0
44	34.31452420261944	36.0	33.0	40.0	23.0	41.0
45	34.25320655543965	36.0	33.0	40.0	23.0	41.0
46	34.21570073973764	36.0	33.0	40.0	23.0	41.0
47	34.15627466050438	36.0	33.0	40.0	23.0	41.0
48	34.103111168098394	36.0	33.0	40.0	23.0	41.0
49	34.035355536903644	36.0	33.0	40.0	24.0	41.0
50	33.8982920094031	35.0	33.0	40.0	24.0	41.0
51	33.74140708764915	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	6.0
12	6.0
13	16.0
14	63.0
15	209.0
16	617.0
17	1631.0
18	3768.0
19	7222.0
20	12383.0
21	19970.0
22	30277.0
23	43241.0
24	60345.0
25	84081.0
26	109389.0
27	128544.0
28	141053.0
29	159646.0
30	188866.0
31	228440.0
32	279932.0
33	356657.0
34	565533.0
35	824540.0
36	493756.0
37	607549.0
38	855267.0
39	1409792.0
40	761.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.96305878179698	24.394921283707824	28.45594075566854	14.186079178826658
2	31.383455902198527	25.99387833574076	29.449127330949242	13.17353843111147
3	28.898924497710084	25.295374156222344	32.22055410088453	13.585147245183041
4	25.4366596149941	27.723339967681554	31.37284134825399	15.467159069070355
5	23.371221645948378	32.26130370612745	29.959956519641988	14.407518128282176
6	21.45523417717021	40.581148340508236	27.775883521751748	10.187733960569805
7	85.98325470952788	4.678145404571002	7.507483487337609	1.831116398563497
8	86.62094445034982	4.1126709196452556	7.308498401995537	1.9578862280093885
9	81.18753270741738	6.450896876886748	9.034467210629796	3.327103205066076
10	45.35993544173857	23.131441594021737	18.203733208176352	13.304889756063337
11	38.961037782822295	24.146371372396807	21.322824420913335	15.569766423867565
12	34.14404131148106	23.139258865231604	27.038187143053495	15.678512680233842
13	21.433974828386702	34.888662855003524	27.166529499009684	16.51083281760008
14	17.706829951368107	36.8492556430643	29.062890627303506	16.381023778264083
15	17.589873292164388	24.429441264698397	41.91197450208745	16.068710941049762
16	18.63927769018839	21.04621095957231	40.68079208765142	19.63371926258789
17	18.887026217797036	22.00235243917762	29.672002722890134	29.438618620135205
18	22.013919581296673	24.284043044284314	33.54809912541821	20.153938249000802
19	27.881000870786554	25.042968530871647	26.80743399811388	20.268596600227927
20	29.482452796609877	23.05124274199633	27.809541637251094	19.6567628241427
21	23.128236059212277	27.42088566205105	28.430946656423068	21.019931622313607
22	22.68455677659887	25.23604152135287	27.18311662960393	24.89628507244433
23	21.05036908255628	28.446823125998232	26.990346048066993	23.51246174337849
24	20.645141097209205	24.60350785303107	36.43776779257045	18.31358325718928
25	19.563076533202008	25.932549801838977	33.71791686808363	20.78645679687539
26	18.958893703407288	31.546242636909223	28.585175218010388	20.9096884416731
27	19.562139065474714	32.27330934121572	29.102566680794205	19.061984912515364
28	18.126618927382694	28.74566364474449	34.369577902131695	18.758139525741125
29	18.992037723701348	25.425304159136054	33.49236515698578	22.090292960176825
30	19.892157946377147	30.202307047595085	30.217654301517744	19.68788070451002
31	25.909687685650738	27.964284294043708	26.657560125324313	19.468467894981238
32	26.862230498819017	26.74905395141891	27.92961310858099	18.459102441181084
33	26.486820035378823	28.501846433411593	25.38639924845329	19.624934282756296
34	21.082953646303405	28.288451561874155	27.91249676233424	22.7160980294882
35	21.9875646418019	26.59656424126125	30.01529735644685	21.400573760489998
36	28.103362167522157	27.372636314989762	25.778245638015584	18.745755879472494
37	21.139715805146427	32.73983561957015	27.952686911030227	18.16776166425319
38	21.45370701200155	31.928820192329066	25.270606863685085	21.346865931984297
39	22.089854467207605	31.406922836275342	27.007568237444247	19.495654459072806
40	23.93373252322009	27.148173276091352	27.030127944688193	21.88796625600036
41	19.127275003587325	26.545154720732146	28.200511040874954	26.12705923480558
42	21.842453709884886	26.705567545230174	26.146247082320706	25.305731662564234
43	22.10443057832233	27.07278272628014	27.634613183427202	23.188173511970327
44	20.990461870692656	29.38268808588898	28.269203232570167	21.357646810848195
45	19.296714735072378	34.35655919708006	24.86776790899789	21.47895815884967
46	21.475949189854	32.154129976271484	26.331306235778275	20.038614598096245
47	21.95351339467497	28.24429985600798	27.45777955325429	22.344407196062757
48	22.32766886099637	26.21977781712454	30.20000873961849	21.2525445822606
49	21.740496534317895	26.670835877978593	30.592066815441786	20.99660077226172
50	20.420360528919293	31.92877483098742	27.263391083865407	20.387473556227878
51	20.46839818971958	31.26098027976154	25.86373664656605	22.406884883952834
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3314.0
1	7018.0
2	10722.0
3	30737.0
4	50752.0
5	38304.0
6	25856.0
7	26861.5
8	27867.0
9	29526.5
10	31186.0
11	32222.5
12	33259.0
13	33366.5
14	33474.0
15	32666.5
16	31859.0
17	30905.5
18	29952.0
19	32430.5
20	34909.0
21	34417.0
22	33925.0
23	34592.0
24	35259.0
25	40900.0
26	56978.5
27	67416.0
28	79033.0
29	90650.0
30	108991.0
31	127332.0
32	145534.0
33	163736.0
34	183865.0
35	203994.0
36	218626.5
37	233259.0
38	268369.0
39	303479.0
40	380649.5
41	457820.0
42	514235.5
43	570651.0
44	576873.0
45	583095.0
46	565644.0
47	548193.0
48	530313.0
49	512433.0
50	483589.0
51	454745.0
52	416797.5
53	378850.0
54	340407.0
55	301964.0
56	281191.5
57	260419.0
58	249063.5
59	237708.0
60	218354.0
61	199000.0
62	173881.5
63	148763.0
64	129127.0
65	109491.0
66	92997.0
67	76503.0
68	65046.0
69	53589.0
70	45316.5
71	37044.0
72	32608.5
73	28173.0
74	22368.0
75	12901.0
76	9239.0
77	7322.5
78	5406.0
79	4354.0
80	3302.0
81	2200.5
82	1099.0
83	745.5
84	392.0
85	328.0
86	264.0
87	158.0
88	52.0
89	41.0
90	30.0
91	25.5
92	21.0
93	13.0
94	5.0
95	4.5
96	4.0
97	2.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6613561.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	14.699771526934246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.32511726857838	11.80760871722303
2	8.238416407496127	2.42205677867879
3	2.7851984632695133	1.2282534320169065
4	1.3737098404657444	0.8077288319659096
5	0.8148742768907131	0.5989232846734619
6	0.5788994522632489	0.5105813811202282
7	0.4185021408527527	0.4306320097847825
8	0.3259036826252525	0.3832567739502161
9	0.25317855395060374	0.33495002087341236
>10	2.27453987186391	7.423867908965646
>50	0.8295431895500502	8.893614477437751
>100	1.6787228701766497	54.492975874444085
>500	0.09672340253217114	8.37971206734462
>1k	0.006149440462710897	1.338648178295307
>5k	3.1268341335818125E-4	0.29568478680722804
>10k+	2.0845560890545418E-4	0.6515054764186621
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	27998	0.4233422811099799	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	14525	0.21962449578978707	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	8274	0.1251065802523028	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5120447214443172E-5	0.0	0.0	0.023754222573890222	0.0
2	1.5120447214443172E-5	0.0	0.0	0.07762837599895124	0.0
3	1.5120447214443172E-5	0.0	0.0	0.1124961272754572	0.0
4	1.5120447214443172E-5	0.0	0.0	0.19115269368499058	0.0
5	1.5120447214443172E-5	0.0	0.0	0.3366869981240061	0.0
6	1.5120447214443172E-5	0.0	0.0	0.521504224426145	0.0
7	1.5120447214443172E-5	0.0	0.0	0.6343481219875344	0.0
8	1.5120447214443172E-5	0.0	0.0	0.892817046671226	0.0
9	3.0240894428886345E-5	0.0	0.0	1.0094410560362261	0.0
10	3.0240894428886345E-5	0.0	0.0	1.2153664266497277	0.0
11	3.0240894428886345E-5	0.0	0.0	1.3858343485453601	0.0
12	3.0240894428886345E-5	0.0	0.0	1.5495736714305652	0.0
13	4.5361341643329514E-5	0.0	0.0	1.6164967708016906	0.0
14	4.5361341643329514E-5	0.0	0.0	1.6454977885589928	0.0
15	4.5361341643329514E-5	0.0	0.0	1.6841456516391093	0.0
16	4.5361341643329514E-5	0.0	0.0	1.7665067276161814	0.0
17	4.5361341643329514E-5	0.0	0.0	1.8610851249425233	0.0
18	4.5361341643329514E-5	0.0	0.0	1.985299598809174	0.0
19	4.5361341643329514E-5	0.0	0.0	2.052842636516092	0.0
20	4.5361341643329514E-5	0.0	0.0	2.1212021783725894	0.0
21	4.5361341643329514E-5	0.0	0.0	2.214767505735564	0.0
22	6.048178885777269E-5	0.0	0.0	2.3213817790446023	0.0
23	6.048178885777269E-5	0.0	0.0	2.4358889257995804	0.0
24	6.048178885777269E-5	0.0	0.0	2.5270803429498874	0.0
25	6.048178885777269E-5	0.0	0.0	2.6044667917934077	0.0
26	6.048178885777269E-5	0.0	0.0	2.682306854053361	0.0
27	6.048178885777269E-5	0.0	0.0	2.7590884850083035	0.0
28	7.560223607221587E-5	0.0	0.0	2.839907275369502	0.0
29	7.560223607221587E-5	0.0	0.0	2.932202485166463	0.0
30	1.058431305011022E-4	0.0	0.0	3.0444264443920606	0.0
31	1.2096357771554538E-4	0.0	0.0	3.1502544544459483	0.0
32	1.3608402492998854E-4	0.0	0.0	3.253118856845805	0.0
33	1.3608402492998854E-4	0.0	0.0	3.3528230857778434	0.0
34	1.5120447214443173E-4	0.0	0.0	3.4591954319314513	0.0
35	1.8144536657331805E-4	0.0	0.0	3.5850580345444762	0.0
36	1.8144536657331805E-4	0.0	0.0	3.7004270467906775	0.0
37	1.8144536657331805E-4	0.0	0.0	3.816385756478242	0.0
38	1.8144536657331805E-4	0.0	0.0	3.935444157844768	0.0
39	1.8144536657331805E-4	0.0	0.0	4.055440026938589	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGAA	20	7.035721E-4	45.000004	31
GTCGACG	55	6.184564E-11	40.909092	1
TACGTAG	170	0.0	39.705883	1
CGTTTTT	25435	0.0	39.117355	1
TAGGGCG	2480	0.0	39.10282	5
TGTTACG	185	0.0	38.91892	1
TAACGGG	1730	0.0	38.106937	3
TTCGCGA	65	9.094947E-12	38.07692	15
TAGGGTA	3510	0.0	37.948715	5
TATCGTG	270	0.0	37.500004	1
CTATACG	180	0.0	37.5	1
CCGTAGA	90	0.0	37.5	34
AGGGCGA	5840	0.0	37.21747	6
TTACGCG	195	0.0	36.92308	1
ATAGGGC	3235	0.0	36.79289	4
GGACTAA	4245	0.0	36.78445	8
TATACGG	710	0.0	36.760563	2
CGACCCG	940	0.0	36.62234	32
GCTACGA	1150	0.0	36.586956	10
CGAATAT	1075	0.0	36.209305	14
>>END_MODULE