##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549017_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	777845
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.269395573668277	31.0	31.0	33.0	30.0	34.0
2	31.626079745964812	31.0	31.0	34.0	30.0	34.0
3	31.74698301075407	31.0	31.0	34.0	30.0	34.0
4	35.46117414137778	37.0	35.0	37.0	33.0	37.0
5	35.30968252029646	37.0	35.0	37.0	33.0	37.0
6	35.39296903624758	37.0	35.0	37.0	33.0	37.0
7	35.79971716730197	37.0	35.0	37.0	35.0	37.0
8	35.81949617211655	37.0	35.0	37.0	35.0	37.0
9	37.472015632934585	39.0	37.0	39.0	35.0	39.0
10	36.83882393021746	39.0	37.0	39.0	32.0	39.0
11	36.53015703642757	39.0	35.0	39.0	32.0	39.0
12	35.91356375627535	37.0	35.0	39.0	31.0	39.0
13	35.63172482949688	37.0	35.0	39.0	30.0	39.0
14	36.66345608700962	38.0	35.0	41.0	31.0	41.0
15	36.841805243975344	38.0	35.0	41.0	31.0	41.0
16	36.97305375749667	38.0	35.0	41.0	32.0	41.0
17	36.92802036395426	38.0	35.0	41.0	32.0	41.0
18	36.845557919636946	38.0	35.0	40.0	31.0	41.0
19	36.79901394236641	38.0	35.0	40.0	31.0	41.0
20	36.66764843895635	38.0	35.0	40.0	31.0	41.0
21	36.48543347324981	38.0	35.0	40.0	31.0	41.0
22	36.40475673173962	38.0	35.0	40.0	31.0	41.0
23	36.34255925023623	38.0	35.0	40.0	31.0	41.0
24	36.26393947380262	38.0	35.0	40.0	30.0	41.0
25	36.1638552667948	38.0	35.0	40.0	30.0	41.0
26	36.08187749487366	38.0	35.0	40.0	30.0	41.0
27	36.01992170676677	38.0	34.0	40.0	30.0	41.0
28	35.97761636315718	38.0	35.0	40.0	30.0	41.0
29	35.959770905514596	38.0	35.0	40.0	30.0	41.0
30	35.87997737338416	37.0	35.0	40.0	30.0	41.0
31	35.76260823171712	38.0	34.0	40.0	30.0	41.0
32	35.604836439136335	37.0	34.0	40.0	29.0	41.0
33	35.425902332726956	37.0	34.0	40.0	28.0	41.0
34	35.25789328208062	37.0	34.0	40.0	27.0	41.0
35	35.125339881338824	37.0	34.0	40.0	27.0	41.0
36	34.942677525728136	37.0	34.0	40.0	26.0	41.0
37	34.883717193014036	37.0	34.0	40.0	26.0	41.0
38	34.87728146353065	37.0	34.0	40.0	26.0	41.0
39	34.79496429237187	37.0	34.0	40.0	25.0	41.0
40	34.6388316438365	37.0	33.0	40.0	24.0	41.0
41	34.586885562033565	36.0	33.0	40.0	24.0	41.0
42	34.5036388997808	36.0	33.0	40.0	24.0	41.0
43	34.44436873670204	36.0	33.0	40.0	24.0	41.0
44	34.380539824772285	36.0	33.0	40.0	24.0	41.0
45	34.34529372818492	36.0	33.0	40.0	24.0	41.0
46	34.293280795017004	36.0	33.0	40.0	24.0	41.0
47	34.22694367129698	35.0	33.0	40.0	24.0	41.0
48	34.18283976884855	35.0	33.0	40.0	24.0	41.0
49	34.11858917907809	36.0	33.0	40.0	24.0	41.0
50	34.017104950215014	35.0	33.0	40.0	24.0	41.0
51	33.86258444805842	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	1.0
12	3.0
13	2.0
14	9.0
15	25.0
16	62.0
17	198.0
18	445.0
19	833.0
20	1387.0
21	2314.0
22	3544.0
23	4843.0
24	6886.0
25	9563.0
26	12356.0
27	14882.0
28	16130.0
29	18680.0
30	21848.0
31	26636.0
32	32676.0
33	42231.0
34	68119.0
35	100029.0
36	58423.0
37	71782.0
38	99665.0
39	164177.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.57481246263716	23.833154420225107	28.313867158624145	14.278165958513586
2	31.13705172624366	26.002095533171776	29.456896939621647	13.403955800962915
3	29.036376141776316	25.05974840745907	31.74167089844378	14.162204552320834
4	26.23298986301898	28.320937976074923	30.804594745739834	14.64147741516626
5	22.99802659912965	32.78300946846737	29.713503332926226	14.505460599476761
6	21.90744942758519	41.0010991907128	26.48933913568899	10.602112246013023
7	85.55033457822574	5.499938933849289	6.8823480256349265	2.067378462290045
8	86.95369900172913	4.399591178191028	6.696064125886263	1.9506456941935733
9	81.57974917882098	6.53922053879629	8.600942347125713	3.2800879352570242
10	46.465555476990914	25.103073234384745	16.256323560606546	12.175047728017793
11	39.7073967178551	23.066163567291685	21.792773624565307	15.433666090287911
12	36.118249779840454	22.43236120306745	26.292770410557374	15.156618606534721
13	22.87293741040953	35.78682128187493	25.459056752952065	15.88118455476348
14	17.586794284208292	36.989760170728104	27.374734040843613	18.04871150421999
15	17.11639208325566	23.79985729804781	41.47728660594334	17.606464012753182
16	18.050897029613868	19.955518130218746	40.46397418508828	21.529610655079097
17	18.699869511277953	21.312343718864298	29.16455077811132	30.82323599174643
18	21.835712770539118	23.663711922041024	32.990505820568366	21.510069486851492
19	27.942070721030536	25.32985363407877	25.135470434341034	21.59260521054966
20	29.6658074552128	23.05947843079277	26.8170393844532	20.457674729541235
21	22.967429243615374	27.369205947200275	27.7349600498814	21.928404759302946
22	23.513296350815395	25.572189832164504	25.474741111661064	25.439772705359037
23	20.882566578174313	28.565202578919962	26.004538179200225	24.547692663705494
24	20.358554724913063	23.493112381001357	37.25870835449222	18.889624539593363
25	19.44500511027261	26.19737865513052	32.631565414703445	21.726050819893423
26	19.467760286432387	32.16836259151887	26.54976248481381	21.814114637234923
27	19.108177078981033	32.62038066709948	27.958783562277834	20.31265869164165
28	17.994459050324938	28.104570962081134	33.72342818942077	20.177541798173156
29	17.935321304372977	24.06507723261061	34.334218256850654	23.665383206165753
30	18.851956366628315	30.669734972905914	30.235201100476317	20.24310755998946
31	26.09761584891592	28.494365844094904	25.80295560169443	19.605062705294756
32	27.056932936510485	26.274000604233493	26.413874229441596	20.255192229814423
33	26.466069718260066	28.26938528884289	24.94173003618973	20.32281495670731
34	20.017998444420158	26.99213853659791	27.74370215145691	25.24616086752502
35	22.09180492257455	26.270015234397597	29.026991238614375	22.611188604413478
36	27.127512550700978	27.61411335163175	25.30645565633256	19.951918441334712
37	20.44713278352371	32.880201068336234	27.67273685631456	18.99992929182549
38	21.061651100154915	30.713574041100735	26.599001086334685	21.62577377240967
39	21.534110266184136	31.78538140632131	26.94482833983634	19.73567998765821
40	24.13064299442691	27.64663911190533	26.17012386786571	22.052594025802055
41	18.874840103105374	25.978826115742855	28.351792452223773	26.794541328928002
42	20.73523645456357	26.77821416863257	25.899247279342287	26.587302097461578
43	22.117259865397347	26.950999235066114	26.287756558183183	24.643984341353352
44	20.331428497965533	29.018120576721586	27.827009237058796	22.823441688254086
45	18.019656872513163	35.05197050826322	24.29211475293921	22.636257866284414
46	20.64164454357873	32.852432039802274	25.96494160147587	20.54098181514312
47	21.56560754391942	28.62395464392006	27.410859490001222	22.3995783221593
48	21.829284754674774	25.464070605326256	30.659450147522964	22.04719449247601
49	20.878581208338424	26.871677519300118	31.03600331685619	21.213737955505273
50	19.980201711137823	32.080555894811944	27.487995680373338	20.45124671367689
51	19.336757323117073	31.68793268581787	25.77531513347775	23.19999485758731
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	387.0
1	753.5
2	1120.0
3	3441.0
4	5762.0
5	4334.0
6	2906.0
7	2939.0
8	2972.0
9	3147.0
10	3322.0
11	3444.0
12	3566.0
13	3493.0
14	3420.0
15	3322.0
16	3224.0
17	3133.5
18	3043.0
19	3021.0
20	2999.0
21	3239.5
22	3480.0
23	3361.0
24	3242.0
25	4147.0
26	5916.0
27	6780.0
28	9556.5
29	12333.0
30	13321.0
31	14309.0
32	16807.0
33	19305.0
34	21908.0
35	24511.0
36	26093.0
37	27675.0
38	30380.5
39	33086.0
40	42819.0
41	52552.0
42	59166.0
43	65780.0
44	67201.5
45	68623.0
46	66420.5
47	64218.0
48	60325.0
49	56432.0
50	54869.0
51	53306.0
52	49773.0
53	46240.0
54	41796.0
55	37352.0
56	34128.0
57	30904.0
58	29147.5
59	27391.0
60	25600.0
61	23809.0
62	22279.5
63	20750.0
64	18269.0
65	15788.0
66	13289.0
67	10790.0
68	9372.0
69	7954.0
70	6455.0
71	4956.0
72	4231.0
73	3506.0
74	2982.5
75	1852.5
76	1246.0
77	1011.5
78	777.0
79	521.0
80	265.0
81	203.0
82	141.0
83	108.5
84	76.0
85	46.0
86	16.0
87	11.5
88	7.0
89	4.5
90	2.0
91	2.0
92	2.0
93	2.5
94	3.0
95	4.0
96	5.0
97	3.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	777845.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.47980459066621
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.44371739865491	15.99036502725886
2	5.870084021355392	2.5217651541901183
3	2.473566315525238	1.593951632986089
4	1.556774766046361	1.3375687106542375
5	1.1583345146056683	1.2440399512176974
6	0.9134173159430057	1.177201527371195
7	0.7981862145002624	1.2001418740100456
8	0.7663355682479137	1.3168590605473869
9	0.6639504707471373	1.2835373732576416
>10	9.889932408255325	49.88214499781691
>50	1.3844737336122115	19.092690210766413
>100	0.07821885500633964	2.3043570463546006
>500	6.016835000487665E-4	0.07586413831801181
>1k	0.002406734000195066	0.9795132952507862
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3133	0.40277947405974196	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	2072	0.2663769774183803	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	1351	0.173684988654552	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1023	0.13151720458446092	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2856031728686307E-4	0.0	0.0	0.01838412537202142	0.0
2	1.2856031728686307E-4	0.0	0.0	0.056437979288932884	0.0
3	1.2856031728686307E-4	0.0	0.0	0.08292140465002668	0.0
4	1.2856031728686307E-4	0.0	0.0	0.12573199030655208	0.0
5	1.2856031728686307E-4	0.0	0.0	0.21045323939859484	0.0
6	1.2856031728686307E-4	0.0	0.0	0.3118873297379298	0.0
7	1.2856031728686307E-4	0.0	0.0	0.37012515346887875	0.0
8	1.2856031728686307E-4	0.0	0.0	0.5178409580314844	0.0
9	1.2856031728686307E-4	0.0	0.0	0.5859779261935218	0.0
10	1.2856031728686307E-4	0.0	0.0	0.7039962974628622	0.0
11	1.2856031728686307E-4	0.0	0.0	0.8074873528787869	0.0
12	1.2856031728686307E-4	0.0	0.0	0.9042932717957948	0.0
13	1.2856031728686307E-4	0.0	0.0	0.9462039352313122	0.0
14	1.2856031728686307E-4	0.0	0.0	0.9643309399687598	0.0
15	1.2856031728686307E-4	0.0	0.0	0.9914571669162879	0.0
16	1.2856031728686307E-4	0.0	0.0	1.0431384144656068	0.0
17	2.5712063457372614E-4	0.0	0.0	1.106004409618883	0.0
18	2.5712063457372614E-4	0.0	0.0	1.1948395888641052	0.0
19	3.856809518605892E-4	0.0	0.0	1.2371359332514833	0.0
20	3.856809518605892E-4	0.0	0.0	1.2789180363697137	0.0
21	3.856809518605892E-4	0.0	0.0	1.3458979616761695	0.0
22	3.856809518605892E-4	0.0	0.0	1.414806291741928	0.0
23	3.856809518605892E-4	0.0	0.0	1.496570653536373	0.0
24	3.856809518605892E-4	0.0	0.0	1.563936259794689	0.0
25	3.856809518605892E-4	0.0	0.0	1.6257737724096704	0.0
26	5.142412691474523E-4	0.0	0.0	1.6822117516986033	0.0
27	5.142412691474523E-4	0.0	0.0	1.737749808766528	0.0
28	5.142412691474523E-4	0.0	0.0	1.7963733134493376	0.0
29	5.142412691474523E-4	0.0	0.0	1.8556396197185814	0.0
30	5.142412691474523E-4	0.0	0.0	1.9379182227821738	0.0
31	5.142412691474523E-4	0.0	0.0	2.014925852837005	0.0
32	5.142412691474523E-4	0.0	0.0	2.0876909924213694	0.0
33	5.142412691474523E-4	0.0	0.0	2.160970373274881	0.0
34	5.142412691474523E-4	0.0	0.0	2.236049598570409	0.0
35	5.142412691474523E-4	0.0	0.0	2.33478392224672	0.0
36	5.142412691474523E-4	0.0	0.0	2.4177053268967468	0.0
37	5.142412691474523E-4	0.0	0.0	2.503069377575224	0.0
38	5.142412691474523E-4	0.0	0.0	2.5796913266781942	0.0
39	5.142412691474523E-4	0.0	0.0	2.6664695408468266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTTACG	30	2.1645938E-6	45.000004	1
ATAACGG	30	2.1645938E-6	45.000004	2
CCAACGG	60	0.0	45.000004	2
TACGTAG	30	2.1645938E-6	45.000004	1
TGATTCG	50	2.1827873E-11	45.0	15
TACTCGC	40	6.8102963E-9	45.0	45
TCGTAAG	20	7.0318783E-4	45.0	1
TATAGCG	20	7.0318783E-4	45.0	1
TTACGCA	20	7.0318783E-4	45.0	29
AATACGG	40	6.8102963E-9	45.0	2
ATCGACT	25	3.889638E-5	45.0	17
GCGTAAG	65	0.0	41.538464	1
ACGGGTA	175	0.0	41.142857	5
TCGAACG	55	6.002665E-11	40.909092	1
AGGGTAT	620	0.0	40.64516	6
AGGGCGC	100	0.0	40.5	6
CGTTTTT	2645	0.0	40.406425	1
CGTAAGG	140	0.0	40.178574	2
TATGGGC	225	0.0	40.0	4
GCGATAC	45	1.9268555E-8	40.0	9
>>END_MODULE