FastQCFastQC Report
Sat 18 Jun 2016
SRR3549016_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3549016_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038422
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT77380.3796073629503606No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC58250.2857602596518287No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG25840.12676472290821036No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT21620.10606243456948561No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTATCG207.0345734E-445.041
CGAGTTA253.891871E-545.023
ATTACGC253.891871E-545.011
GTAATCG502.1827873E-1145.036
CGTTAGT351.2125565E-745.044
TTATACG253.891871E-545.01
TACGATT207.0345734E-445.017
CGTGTAA253.891871E-545.023
TTACGTA253.891871E-545.030
CGGTCAT351.2125565E-745.010
TATACGT253.891871E-545.028
GTACGAT207.0345734E-445.01
TAACGCG302.1663327E-644.9999961
TATTGCG1100.042.9545441
TAATACG950.042.631581
TAACGGG4650.042.0967753
GCTACGA3400.041.02941510
CGTTTTT61400.040.822481
CGTTAGG3400.040.367652
GCGTTAG1750.039.8571431
Produced by FastQC (version 0.11.5)