##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549016_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2038422
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36941908986461	31.0	31.0	33.0	30.0	34.0
2	31.719000285514973	31.0	31.0	34.0	30.0	34.0
3	31.826399538466518	31.0	31.0	34.0	30.0	34.0
4	35.533525442719906	37.0	35.0	37.0	33.0	37.0
5	35.39428489292207	37.0	35.0	37.0	33.0	37.0
6	35.45625194390563	37.0	35.0	37.0	33.0	37.0
7	35.870785833355406	37.0	35.0	37.0	35.0	37.0
8	35.89525230791269	37.0	35.0	37.0	35.0	37.0
9	37.55503521841895	39.0	37.0	39.0	35.0	39.0
10	36.972881964578484	39.0	37.0	39.0	33.0	39.0
11	36.61811440418127	39.0	35.0	39.0	32.0	39.0
12	35.73256862416124	37.0	35.0	39.0	31.0	39.0
13	35.33523382302585	37.0	35.0	39.0	30.0	39.0
14	36.30141109152079	38.0	35.0	40.0	31.0	41.0
15	36.562694083953176	38.0	35.0	41.0	31.0	41.0
16	36.73432390349005	38.0	35.0	41.0	32.0	41.0
17	36.70040207572328	38.0	35.0	40.0	32.0	41.0
18	36.65003419311605	38.0	35.0	40.0	31.0	41.0
19	36.60799235879519	38.0	35.0	40.0	31.0	41.0
20	36.44619563564365	37.0	35.0	40.0	31.0	41.0
21	36.24509890493725	37.0	35.0	40.0	31.0	41.0
22	36.166817763937004	37.0	35.0	40.0	31.0	41.0
23	36.12443988536231	37.0	35.0	40.0	31.0	41.0
24	36.077169987372585	37.0	35.0	40.0	31.0	41.0
25	35.954712027244604	37.0	34.0	40.0	30.0	41.0
26	35.86147569050962	37.0	34.0	40.0	30.0	41.0
27	35.804461980885215	37.0	34.0	40.0	30.0	41.0
28	35.80738139600142	36.0	34.0	40.0	30.0	41.0
29	35.83589610002247	36.0	35.0	40.0	30.0	41.0
30	35.76866468277913	36.0	35.0	40.0	30.0	41.0
31	35.59765053556133	36.0	34.0	40.0	30.0	41.0
32	35.406354032678216	36.0	34.0	40.0	29.0	41.0
33	35.24670406814683	36.0	34.0	40.0	29.0	41.0
34	35.08232838931291	36.0	34.0	40.0	27.0	41.0
35	34.9523234148768	36.0	34.0	40.0	27.0	41.0
36	34.770143277496025	36.0	34.0	40.0	26.0	41.0
37	34.68614055382055	36.0	33.0	40.0	26.0	41.0
38	34.690578790849	35.0	33.0	40.0	27.0	41.0
39	34.61694290976059	36.0	33.0	40.0	26.0	41.0
40	34.44489659157917	35.0	33.0	40.0	24.0	41.0
41	34.4438963080265	35.0	33.0	40.0	25.0	41.0
42	34.373144520614474	35.0	33.0	40.0	25.0	41.0
43	34.28039826885699	35.0	33.0	40.0	24.0	41.0
44	34.202937370181445	35.0	33.0	40.0	24.0	41.0
45	34.15886749652427	35.0	33.0	40.0	24.0	41.0
46	34.14763478808608	35.0	33.0	40.0	24.0	41.0
47	34.095346792764204	35.0	33.0	40.0	24.0	41.0
48	34.05939005760338	35.0	33.0	39.0	24.0	41.0
49	34.02087644266006	35.0	33.0	39.0	24.0	41.0
50	33.89630115844511	35.0	33.0	39.0	24.0	41.0
51	33.72704327170723	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	4.0
13	6.0
14	20.0
15	59.0
16	158.0
17	453.0
18	1084.0
19	2124.0
20	3521.0
21	5650.0
22	8339.0
23	12158.0
24	17174.0
25	24303.0
26	31918.0
27	38052.0
28	42820.0
29	48907.0
30	58276.0
31	71221.0
32	89622.0
33	115883.0
34	201518.0
35	316230.0
36	143776.0
37	171891.0
38	238734.0
39	394252.0
40	268.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.12812557949238	24.509448975727302	28.152953608232252	14.209471836548074
2	29.829495560781822	26.552892384403233	30.288527105771035	13.329084949043917
3	28.01887930958359	26.607591558568345	31.243972052891895	14.129557078956173
4	25.999817505894264	28.01245276983863	31.006680657881443	14.981049066385665
5	23.29772735969294	32.278350606498556	29.17413567946186	15.249786354346648
6	22.29788532502102	40.52683889793183	26.97194202181884	10.203333755228309
7	86.1516898856076	5.203485833649755	6.564980166030391	2.079844114712263
8	87.3852421137527	4.119313861408481	6.504835603226418	1.990608421612404
9	82.11930601219963	5.869834607358045	8.492059053522775	3.518800326919549
10	51.97564586724437	21.493096130241923	14.9891926205663	11.542065381947408
11	46.809051315184	20.554183579258858	19.059890444667495	13.576874660889649
12	40.95609250685089	21.601611442576658	23.865323274572194	13.57697277600026
13	21.84783131265263	39.81781005110816	23.819846920804427	14.514511715434782
14	16.231231805779174	41.055532171454196	27.76250452555948	14.950731497207153
15	15.669719027757745	22.854786692843778	46.61345884218282	14.862035437215651
16	16.65597211961017	18.880830367804116	44.25168095713253	20.211516555453187
17	17.357642333138084	19.219523729630076	29.200921104658406	34.22191283257343
18	21.703111524502777	23.051409374506356	34.3423981884026	20.90308091258827
19	30.056779214510048	23.475070422120638	25.791224780737252	20.676925582632055
20	31.701433756111346	23.022465416876386	24.891509216442913	20.384591610569352
21	22.846005390444176	27.955349775463567	27.650064608800335	21.548580225291918
22	23.41747685219253	25.350589818987434	24.31321875450716	26.918714574312872
23	20.74496841184014	29.689926815938993	24.884493986034293	24.68061078618657
24	20.149507805547625	23.055530209151982	38.52808692213879	18.266875063161603
25	17.563831238085147	25.372763833985307	35.689763944855386	21.373640983074164
26	17.266738683157854	33.40907819872431	28.1232247297174	21.20095838840044
27	18.362684468672335	34.20901069552821	28.306847159224148	19.12145767657531
28	16.556630570117473	28.417619119102916	36.80234024161827	18.223410069161343
29	16.87599525515325	24.19420512533715	35.78405256615166	23.14574705335794
30	18.421553535038377	31.101508912286075	31.018895989152394	19.458041563523157
31	28.770882574854472	26.86946078878662	25.387235812800295	18.97242082355862
32	30.401212310306697	25.52131011144895	26.549605528197795	17.527872050046554
33	28.84186885738086	26.84856227022668	24.23330399691526	20.076264875477207
34	19.768821176380552	26.890555537567785	28.516371977931946	24.824251308119713
35	21.17476165386755	25.79446257938739	30.6311941295767	22.39958163716836
36	30.231620341617194	25.929125568699714	24.888712935790526	18.95054115389257
37	21.308492549629076	33.560469814395645	27.60753170835087	17.52350592762441
38	21.229362712922054	33.50071771203411	24.092116352747368	21.17780322229646
39	21.375407055065143	33.268626417886	25.80299859401046	19.5529679330384
40	24.57915976181576	26.970470295159686	25.062229508904437	23.388140434120118
41	18.45461832731397	25.523959219435426	26.234312620252332	29.787109832998272
42	21.09406197539077	25.94325414462756	25.00017170144357	27.962512178538105
43	22.187996401137745	25.8500938471033	26.38266266749476	25.5792470842642
44	20.077147911472697	30.133505231007124	27.863710262153763	21.925636595366417
45	17.546661093728382	37.172430438839456	23.21678239343963	22.06412607399253
46	21.554614304594434	33.550952648666474	24.998896205005636	19.895536841733456
47	21.64713685389973	27.31505056362225	27.431120739473965	23.60669184300405
48	22.938527939749473	24.210933751696164	31.18304256920304	21.667495739351324
49	21.249917828594864	24.85829725150141	32.34693306881499	21.544851851088733
50	19.517303090331637	33.55178662710666	26.97885913711685	19.95205114544486
51	19.422965411480057	32.67360732959123	24.96725408183389	22.936173177094833
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1357.0
1	2384.5
2	3412.0
3	8789.0
4	14166.0
5	10558.5
6	6951.0
7	7279.0
8	7607.0
9	8047.0
10	8487.0
11	8887.5
12	9288.0
13	8867.0
14	8446.0
15	8439.0
16	8432.0
17	7937.0
18	7442.0
19	7453.5
20	7465.0
21	7641.5
22	7818.0
23	8591.0
24	9364.0
25	10255.0
26	13737.0
27	16328.0
28	19699.0
29	23070.0
30	29118.5
31	35167.0
32	38536.0
33	41905.0
34	46805.5
35	51706.0
36	57423.0
37	63140.0
38	73813.0
39	84486.0
40	113762.0
41	143038.0
42	174358.5
43	205679.0
44	206695.5
45	207712.0
46	196093.0
47	184474.0
48	170308.0
49	156142.0
50	147223.0
51	138304.0
52	125008.5
53	111713.0
54	104089.0
55	96465.0
56	87300.0
57	78135.0
58	71004.0
59	63873.0
60	62305.0
61	60737.0
62	53196.0
63	45655.0
64	42761.5
65	39868.0
66	33046.0
67	26224.0
68	22262.5
69	18301.0
70	15882.5
71	13464.0
72	11219.0
73	8974.0
74	7554.5
75	4687.0
76	3239.0
77	2507.5
78	1776.0
79	1279.5
80	783.0
81	544.0
82	305.0
83	234.5
84	164.0
85	94.0
86	24.0
87	26.0
88	28.0
89	20.0
90	12.0
91	9.5
92	7.0
93	5.5
94	4.0
95	2.5
96	1.0
97	2.0
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2038422.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.863608666925996
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.34068321848729	12.744931586115507
2	7.091497503348647	2.249934825112113
3	2.396281135069361	1.1404099854803273
4	1.256200329844502	0.7971148175966614
5	0.7321266483607276	0.580708532211136
6	0.4999015156024077	0.47581452093118776
7	0.3671342137068762	0.4076851446139823
8	0.28296548292478824	0.35910829498932567
9	0.23880826988072215	0.3409524845832083
>10	3.3521851442377333	13.538107349692726
>50	1.690432854095121	19.59674406700951
>100	1.741195125282547	45.866789742724706
>500	0.008719989895883335	0.8710375621399794
>1k	0.001245712842269048	0.3605979902478424
>5k	6.22856421134524E-4	0.6700630965517861
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7738	0.3796073629503606	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	5825	0.2857602596518287	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2584	0.12676472290821036	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2162	0.10606243456948561	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022958935882756368	0.0
2	0.0	0.0	0.0	0.07785434026909051	0.0
3	0.0	0.0	0.0	0.11307766497810562	0.0
4	0.0	0.0	0.0	0.1928943074593975	0.0
5	0.0	0.0	0.0	0.3320215343044767	0.0
6	0.0	0.0	0.0	0.5061267980820459	0.0
7	0.0	0.0	0.0	0.6168006428502048	0.0
8	0.0	0.0	0.0	0.9066326795923514	0.0
9	0.0	0.0	0.0	1.0392352515818608	0.0
10	0.0	0.0	0.0	1.2537148833754739	0.0
11	0.0	0.0	0.0	1.4233559096202848	0.0
12	0.0	0.0	0.0	1.5748946979575378	0.0
13	0.0	0.0	0.0	1.6330769585493092	0.0
14	0.0	0.0	0.0	1.653779246888034	0.0
15	0.0	0.0	0.0	1.687481787382593	0.0
16	0.0	0.0	0.0	1.7562604799202521	0.0
17	0.0	0.0	0.0	1.8379903670584403	0.0
18	0.0	0.0	0.0	1.9640682841923802	0.0
19	4.9057555305034975E-5	0.0	0.0	2.021367508788661	0.0
20	9.811511061006995E-5	0.0	0.0	2.0812177262608036	0.0
21	1.4717266591510493E-4	0.0	0.0	2.1610834262974006	0.0
22	1.4717266591510493E-4	0.0	0.0	2.245707709198586	0.0
23	1.4717266591510493E-4	0.0	0.0	2.3461775824632976	0.0
24	1.4717266591510493E-4	0.0	0.0	2.4247677860619636	0.0
25	1.4717266591510493E-4	0.0	0.0	2.495508780811824	0.0
26	1.4717266591510493E-4	0.0	0.0	2.556879782498423	0.0
27	1.4717266591510493E-4	0.0	0.0	2.6181036115191065	0.0
28	1.4717266591510493E-4	0.0	0.0	2.683791678072548	0.0
29	1.4717266591510493E-4	0.0	0.0	2.7558081692603396	0.0
30	1.4717266591510493E-4	0.0	0.0	2.8570139058546267	0.0
31	1.4717266591510493E-4	0.0	0.0	2.9438457787445387	0.0
32	1.4717266591510493E-4	0.0	0.0	3.028470061645724	0.0
33	1.962302212201399E-4	0.0	0.0	3.1086791645694563	0.0
34	1.962302212201399E-4	0.0	0.0	3.1933034474706417	0.0
35	1.962302212201399E-4	0.0	0.0	3.3125133068618764	0.0
36	1.962302212201399E-4	0.0	0.0	3.408960460591575	0.0
37	1.962302212201399E-4	0.0	0.0	3.5034943696643777	0.0
38	2.4528777652517485E-4	0.0	0.0	3.5970961851863845	0.0
39	2.4528777652517485E-4	0.0	0.0	3.694671662688099	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTATCG	20	7.0345734E-4	45.0	41
CGAGTTA	25	3.891871E-5	45.0	23
ATTACGC	25	3.891871E-5	45.0	11
GTAATCG	50	2.1827873E-11	45.0	36
CGTTAGT	35	1.2125565E-7	45.0	44
TTATACG	25	3.891871E-5	45.0	1
TACGATT	20	7.0345734E-4	45.0	17
CGTGTAA	25	3.891871E-5	45.0	23
TTACGTA	25	3.891871E-5	45.0	30
CGGTCAT	35	1.2125565E-7	45.0	10
TATACGT	25	3.891871E-5	45.0	28
GTACGAT	20	7.0345734E-4	45.0	1
TAACGCG	30	2.1663327E-6	44.999996	1
TATTGCG	110	0.0	42.954544	1
TAATACG	95	0.0	42.63158	1
TAACGGG	465	0.0	42.096775	3
GCTACGA	340	0.0	41.029415	10
CGTTTTT	6140	0.0	40.82248	1
CGTTAGG	340	0.0	40.36765	2
GCGTTAG	175	0.0	39.857143	1
>>END_MODULE