##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549014_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3924724
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.282492221109052	31.0	31.0	33.0	30.0	34.0
2	31.623097063640653	31.0	31.0	34.0	30.0	34.0
3	31.726636573680086	31.0	31.0	34.0	30.0	34.0
4	35.42867855166376	37.0	35.0	37.0	33.0	37.0
5	35.26957131253051	37.0	35.0	37.0	33.0	37.0
6	35.345497161074256	37.0	35.0	37.0	32.0	37.0
7	35.82572583447906	37.0	35.0	37.0	35.0	37.0
8	35.8555506068707	37.0	35.0	37.0	35.0	37.0
9	37.501764200489	39.0	37.0	39.0	35.0	39.0
10	36.8872450648759	39.0	37.0	39.0	32.0	39.0
11	36.517534226610586	39.0	35.0	39.0	32.0	39.0
12	35.59661545627157	37.0	35.0	39.0	31.0	39.0
13	35.180699075909544	37.0	35.0	39.0	30.0	39.0
14	36.124681124073945	38.0	35.0	40.0	30.0	41.0
15	36.400637344180126	38.0	35.0	40.0	31.0	41.0
16	36.61463175499729	38.0	35.0	40.0	32.0	41.0
17	36.57543129147425	38.0	35.0	40.0	32.0	41.0
18	36.52267063875065	38.0	35.0	40.0	31.0	41.0
19	36.445868295452115	37.0	35.0	40.0	31.0	41.0
20	36.28650524215206	37.0	35.0	40.0	31.0	41.0
21	36.06703019116758	37.0	34.0	40.0	30.0	41.0
22	35.986057873114135	37.0	34.0	40.0	30.0	41.0
23	35.96700608756183	37.0	34.0	40.0	30.0	41.0
24	35.90039911086741	36.0	34.0	40.0	30.0	41.0
25	35.788656985816075	36.0	34.0	40.0	30.0	41.0
26	35.677590067479905	36.0	34.0	40.0	30.0	41.0
27	35.6202831078058	36.0	34.0	40.0	30.0	41.0
28	35.61546646337424	36.0	34.0	40.0	30.0	41.0
29	35.64916157161625	36.0	34.0	40.0	30.0	41.0
30	35.5839338001857	36.0	34.0	40.0	30.0	41.0
31	35.3992573745313	36.0	34.0	40.0	29.0	41.0
32	35.19969302299983	36.0	34.0	40.0	29.0	41.0
33	35.020123708062016	36.0	34.0	40.0	27.0	41.0
34	34.858066962160905	36.0	34.0	40.0	27.0	41.0
35	34.70996839522983	36.0	34.0	40.0	26.0	41.0
36	34.531269969557094	35.0	33.0	40.0	25.0	41.0
37	34.45321709246306	35.0	33.0	40.0	25.0	41.0
38	34.46135244159844	35.0	33.0	40.0	25.0	41.0
39	34.41857236330504	35.0	33.0	40.0	25.0	41.0
40	34.24723471000763	35.0	33.0	40.0	24.0	41.0
41	34.24026886986193	35.0	33.0	40.0	24.0	41.0
42	34.14960364091844	35.0	33.0	40.0	24.0	41.0
43	34.04634007384978	35.0	33.0	40.0	23.0	41.0
44	34.001484690388416	35.0	33.0	40.0	23.0	41.0
45	33.9522567701576	35.0	33.0	40.0	23.0	41.0
46	33.935214297871646	35.0	33.0	40.0	23.0	41.0
47	33.87882281658532	35.0	33.0	40.0	23.0	41.0
48	33.857556862597214	35.0	33.0	39.0	23.0	41.0
49	33.82639900283434	35.0	33.0	39.0	24.0	41.0
50	33.69005718618685	35.0	33.0	39.0	24.0	41.0
51	33.51983502534191	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	11.0
14	36.0
15	133.0
16	354.0
17	1028.0
18	2314.0
19	4398.0
20	7575.0
21	11981.0
22	18243.0
23	26238.0
24	37147.0
25	51137.0
26	66899.0
27	78547.0
28	86991.0
29	99843.0
30	118912.0
31	144165.0
32	178858.0
33	229832.0
34	387632.0
35	615934.0
36	273115.0
37	315580.0
38	435142.0
39	732198.0
40	479.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.43361826207397	23.74730044711424	29.518406899440574	14.300674391371215
2	29.447497454598082	26.39663324096166	30.716784161128274	13.439085143311988
3	28.567613926482473	25.493716246034115	32.40622780098677	13.532442026496641
4	25.774500321551276	27.784068382897754	31.644951339253407	14.796479956297565
5	23.0015664795792	32.22562911430205	29.94758357530364	14.825220830815109
6	20.925879119143154	40.89816252047277	27.36768241537494	10.808275945009127
7	85.52109651532184	5.1558529975611025	7.342223300288123	1.9808271868289338
8	86.77825498047761	4.238208852393187	6.7288808079243285	2.2546553592048766
9	81.49411270703366	6.112480775718241	9.043718743024986	3.349687774223105
10	52.175541515785575	21.84166326090701	15.08467856593228	10.898116657375143
11	46.94393796863168	20.72510066949931	19.182699216556372	13.14826214531264
12	41.997424532272845	22.54043851236418	23.405238177257814	12.056898778105161
13	21.61194010075613	41.89723914344041	22.89032298831714	13.600497767486324
14	15.429237826659914	42.71637954668914	26.981234858807905	14.873147767843037
15	13.639940031451895	23.58802810082951	48.213326593156616	14.558705274561982
16	15.036191079933264	18.786340135000575	47.07085135158549	19.10661743348067
17	15.260436147866704	19.948663906047916	29.18961944839943	35.60128049768595
18	20.827681131208205	23.77940461545831	34.89654304353631	20.496371209797175
19	29.29984375971406	25.06512559864082	25.357248051073146	20.277782590571974
20	31.838952242246844	22.59228929218972	26.19763835622581	19.371120109337625
21	21.319588332835636	28.83351797476714	28.626726363433452	21.22016732896377
22	21.890074308409968	26.036225732051477	24.797692780435007	27.27600717910355
23	19.004750397734973	31.560104608629803	24.86977428221704	24.565370711418176
24	19.431684877713696	23.229225800336533	40.187182589145124	17.15190673280465
25	16.512855426266917	26.08713377042564	36.41996226995835	20.9800485333491
26	16.627793444838414	35.197404964017856	27.596182559588904	20.57861903155483
27	17.0320511709868	35.698331908180045	28.068980137202004	19.200636783631158
28	14.80636600178764	30.22790901984445	37.33261243338385	17.633112544984055
29	15.279112620403371	25.508112162791573	36.90707932583285	22.305695890972206
30	17.404714318764835	32.868222071157106	30.728046099547385	18.999017510530678
31	27.541758350396105	29.052335909480515	24.70110000091726	18.704805739206122
32	29.05029754958565	28.099300740638068	25.682876044277254	17.167525665499024
33	27.571238130375537	29.232628842181	23.903668130548798	19.29246489689466
34	18.529939939725697	28.771500874965984	27.956768424989885	24.741790760318434
35	19.098336596407798	26.516157569296595	31.014537582770153	23.370968251525458
36	28.4701803235081	26.61364213126834	25.629471015031886	19.286706530191676
37	19.560764018055792	34.24681582704924	28.291772873710354	17.900647281184614
38	18.883901135468378	33.878840907029385	24.77516890359679	22.462089053905444
39	19.633329630312858	33.62654291104292	27.009669979341222	19.730457479303006
40	24.268560031227672	27.100300556166495	25.573110363938966	23.05802904866686
41	16.98764040477751	25.279000510609155	27.567645521060847	30.165713563552494
42	20.67686797848715	26.195243283349352	24.85504713197667	28.272841606186834
43	21.370445412212426	26.638637519479076	26.107619287368998	25.8832977809395
44	18.97878678857418	30.87513924546032	28.05443134345243	22.091642622513074
45	16.67495599690577	38.754725173031275	23.162622390771936	21.40769643929102
46	20.985348269075736	34.89988085786415	24.503863201590736	19.610907671469384
47	20.891456316418683	29.423139053854484	25.947276802139463	23.738127827587366
48	21.92215299725535	25.24478154387417	31.522242073582753	21.31082338528773
49	20.32221883627995	25.363821761734073	32.23554063929082	22.078418762695158
50	18.78282396418194	34.496489434671076	26.897967857102817	19.822718744044167
51	17.80272956773521	34.204010269257154	24.603870233932373	23.389389929075268
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	2436.0
1	4579.5
2	6723.0
3	16939.5
4	27156.0
5	20583.5
6	14011.0
7	15060.0
8	16109.0
9	17625.0
10	19141.0
11	20348.5
12	21556.0
13	21650.0
14	21744.0
15	20744.5
16	19745.0
17	19204.0
18	18663.0
19	18425.5
20	18188.0
21	18111.0
22	18034.0
23	17834.5
24	17635.0
25	20968.5
26	29611.0
27	34920.0
28	39367.5
29	43815.0
30	56785.0
31	69755.0
32	77562.5
33	85370.0
34	100210.5
35	115051.0
36	120059.5
37	125068.0
38	145311.5
39	165555.0
40	234830.5
41	304106.0
42	359761.0
43	415416.0
44	419137.5
45	422859.0
46	398043.5
47	373228.0
48	350808.5
49	328389.0
50	302038.5
51	275688.0
52	241505.5
53	207323.0
54	182565.5
55	157808.0
56	139754.5
57	121701.0
58	115249.5
59	108798.0
60	104246.5
61	99695.0
62	85376.0
63	71057.0
64	62546.5
65	54036.0
66	43825.0
67	33614.0
68	28438.5
69	23263.0
70	19506.0
71	15749.0
72	13688.0
73	11627.0
74	9871.5
75	5942.5
76	3769.0
77	2815.0
78	1861.0
79	1315.5
80	770.0
81	599.0
82	428.0
83	353.5
84	279.0
85	182.5
86	86.0
87	62.0
88	38.0
89	25.5
90	13.0
91	9.5
92	6.0
93	9.0
94	12.0
95	11.0
96	10.0
97	5.0
98	0.0
99	1.0
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3924724.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.398149998736018
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.64721090965926	12.264197005678971
2	8.315822891530008	2.5609657649340347
3	3.028355728621479	1.3989322727653513
4	1.5609931243923114	0.9614562510555362
5	0.96791422310748	0.7452044196659509
6	0.6541594421095677	0.6043707127615556
7	0.47680287020647055	0.5139317480686966
8	0.3608843054942111	0.4445560534551628
9	0.2798205652379361	0.38778471326383535
>10	2.206951373622676	7.586212567655745
>50	0.7979838097344437	8.99628177554591
>100	1.6433752019254475	56.52124159655312
>500	0.055375794097480814	5.226906203029155
>1k	0.003847864846652745	0.9103376099804548
>5k	1.6729847159359764E-4	0.16538478284211106
>10k+	3.345969431871953E-4	0.7122365227443437
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15483	0.39449907815173757	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	12165	0.30995810151236114	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	6420	0.16357838156262708	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.547949868576746E-5	0.0	0.0	0.06250121027618757	0.0
2	2.547949868576746E-5	0.0	0.0	0.18498116045867175	0.0
3	2.547949868576746E-5	0.0	0.0	0.25813280118551013	0.0
4	2.547949868576746E-5	0.0	0.0	0.3969451100255712	0.0
5	2.547949868576746E-5	0.0	0.0	0.65171461738456	0.0
6	2.547949868576746E-5	0.0	0.0	0.9690617735157937	0.0
7	2.547949868576746E-5	0.0	0.0	1.149507583208399	0.0
8	2.547949868576746E-5	0.0	0.0	1.574531100785686	0.0
9	2.547949868576746E-5	0.0	0.0	1.7700607737002654	0.0
10	2.547949868576746E-5	0.0	0.0	2.0738528365306705	0.0
11	2.547949868576746E-5	0.0	0.0	2.3616947331837856	0.0
12	2.547949868576746E-5	0.0	0.0	2.619827534369296	0.0
13	2.547949868576746E-5	0.0	0.0	2.725414576923116	0.0
14	2.547949868576746E-5	0.0	0.0	2.7714305515496123	0.0
15	2.547949868576746E-5	0.0	0.0	2.83278518438494	0.0
16	2.547949868576746E-5	0.0	0.0	2.9548829420871376	0.0
17	2.547949868576746E-5	0.0	0.0	3.098052245202465	0.0
18	5.095899737153492E-5	0.0	0.0	3.292002189198527	0.0
19	5.095899737153492E-5	0.0	0.0	3.3863018138345526	0.0
20	5.095899737153492E-5	0.0	0.0	3.481544179921951	0.0
21	5.095899737153492E-5	0.0	0.0	3.610852635752221	0.0
22	5.095899737153492E-5	0.0	0.0	3.7499452190778255	0.0
23	5.095899737153492E-5	0.0	0.0	3.905370161061007	0.0
24	5.095899737153492E-5	0.0	0.0	4.025862710346002	0.0
25	5.095899737153492E-5	0.0	0.0	4.124162616275692	0.0
26	5.095899737153492E-5	0.0	0.0	4.221494301255324	0.0
27	5.095899737153492E-5	0.0	0.0	4.311972001088484	0.0
28	5.095899737153492E-5	0.0	0.0	4.415979314723787	0.0
29	5.095899737153492E-5	0.0	0.0	4.52281485271321	0.0
30	5.095899737153492E-5	0.0	0.0	4.669296490657687	0.0
31	5.095899737153492E-5	0.0	0.0	4.800312072899903	0.0
32	7.643849605730237E-5	0.0	0.0	4.921263253161241	0.0
33	7.643849605730237E-5	0.0	0.0	5.035003735294508	0.0
34	1.0191799474306984E-4	0.0	0.0	5.156158751545331	0.0
35	1.273974934288373E-4	0.0	0.0	5.329928932582265	0.0
36	1.273974934288373E-4	0.0	0.0	5.468741241422327	0.0
37	1.273974934288373E-4	0.0	0.0	5.599986139152715	0.0
38	1.273974934288373E-4	0.0	0.0	5.72498855970509	0.0
39	1.273974934288373E-4	0.0	0.0	5.853787425561644	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCCGTT	30	2.1668475E-6	45.000004	38
GTCGAAC	40	6.8230293E-9	45.0	29
CCGTCTA	20	7.0353725E-4	45.0	28
TACGATA	45	3.8562575E-10	45.0	44
CCGGATA	115	0.0	44.999996	20
CGGTCTA	395	0.0	42.721516	31
CGACGGT	375	0.0	42.600002	28
ACGTTAG	245	0.0	42.2449	1
GAGTACG	80	0.0	42.1875	1
ATCGTAG	220	0.0	41.93182	1
TATAGCG	205	0.0	41.707317	1
TCACGAC	390	0.0	40.384617	25
CGTTTTT	13100	0.0	40.259544	1
ATCGGCG	45	1.9303116E-8	40.0	11
GTCGGTA	90	0.0	40.0	10
GCGTAAG	315	0.0	39.999996	1
GTTAGCG	255	0.0	39.705883	1
GCGATCG	40	3.461064E-7	39.375	9
AGGGCGA	3495	0.0	39.270386	6
TTAGCGG	980	0.0	39.030613	2
>>END_MODULE