##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549012_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2717557
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.329636508084285	31.0	31.0	33.0	30.0	34.0
2	31.668090126536445	31.0	31.0	34.0	30.0	34.0
3	31.752089836570125	31.0	31.0	34.0	30.0	34.0
4	35.45191214020534	37.0	35.0	37.0	33.0	37.0
5	35.30775141054999	37.0	35.0	37.0	33.0	37.0
6	35.37146414960201	37.0	35.0	37.0	33.0	37.0
7	35.85650162995661	37.0	35.0	37.0	35.0	37.0
8	35.89570338359048	37.0	35.0	37.0	35.0	37.0
9	37.567738965548834	39.0	37.0	39.0	35.0	39.0
10	36.91315030374708	39.0	37.0	39.0	32.0	39.0
11	36.5397884938568	39.0	35.0	39.0	32.0	39.0
12	35.520036930228144	37.0	35.0	39.0	31.0	39.0
13	35.02780438459985	37.0	34.0	39.0	30.0	39.0
14	35.938275811694105	38.0	35.0	40.0	30.0	41.0
15	36.246655727920334	38.0	35.0	40.0	31.0	41.0
16	36.469045911456504	38.0	35.0	40.0	32.0	41.0
17	36.44780624656631	37.0	35.0	40.0	31.0	41.0
18	36.386401462784406	37.0	35.0	40.0	31.0	41.0
19	36.33469399169916	37.0	35.0	40.0	31.0	41.0
20	36.162577270688345	36.0	35.0	40.0	31.0	41.0
21	35.95612640323644	36.0	34.0	40.0	31.0	41.0
22	35.85615168329496	36.0	34.0	40.0	30.0	41.0
23	35.84347559223229	36.0	34.0	40.0	30.0	41.0
24	35.788228912953805	36.0	34.0	40.0	30.0	41.0
25	35.687431395183246	35.0	34.0	40.0	30.0	41.0
26	35.5717267383904	35.0	34.0	40.0	30.0	41.0
27	35.50531929965038	35.0	34.0	40.0	30.0	41.0
28	35.53523881927776	36.0	34.0	40.0	30.0	41.0
29	35.57035933376926	36.0	34.0	40.0	30.0	41.0
30	35.50076005765472	36.0	34.0	40.0	30.0	41.0
31	35.29955912608273	36.0	34.0	40.0	29.0	41.0
32	35.08377046001243	35.0	34.0	40.0	29.0	41.0
33	34.90455471587165	35.0	34.0	40.0	27.0	41.0
34	34.751494816852045	35.0	34.0	40.0	27.0	41.0
35	34.58324554001995	35.0	34.0	40.0	26.0	41.0
36	34.37915856042762	35.0	33.0	40.0	24.0	41.0
37	34.29362769575762	35.0	33.0	40.0	24.0	41.0
38	34.30146598581005	35.0	33.0	40.0	25.0	41.0
39	34.26944016261665	35.0	33.0	40.0	24.0	41.0
40	34.08430255556737	35.0	33.0	40.0	23.0	41.0
41	34.11122636986087	35.0	33.0	40.0	24.0	41.0
42	34.03430139643805	35.0	33.0	40.0	24.0	41.0
43	33.95559541161418	35.0	33.0	40.0	23.0	41.0
44	33.8978078472687	35.0	33.0	40.0	23.0	41.0
45	33.83943409466664	35.0	33.0	39.0	23.0	41.0
46	33.818141808985054	35.0	33.0	39.0	23.0	41.0
47	33.768385354934594	35.0	33.0	39.0	23.0	41.0
48	33.746861611366384	35.0	33.0	39.0	23.0	41.0
49	33.71995214819781	35.0	33.0	39.0	24.0	41.0
50	33.585645857658186	35.0	33.0	39.0	24.0	41.0
51	33.40488166393566	35.0	33.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	7.0
14	27.0
15	96.0
16	208.0
17	703.0
18	1498.0
19	3089.0
20	5379.0
21	8280.0
22	12856.0
23	18597.0
24	26000.0
25	36106.0
26	46999.0
27	54955.0
28	60629.0
29	69362.0
30	82548.0
31	100197.0
32	125010.0
33	161515.0
34	284551.0
35	456947.0
36	182924.0
37	208578.0
38	291273.0
39	478933.0
40	287.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.64811004884166	23.836224962346698	29.792604166168363	13.72306082264328
2	30.13022357948702	26.375932501139808	30.17905420199098	13.314789717382192
3	28.192748118990696	25.026006814208497	32.53572970134573	14.245515365455075
4	25.05275878298045	27.822157916098906	32.31350805153305	14.811575249387593
5	23.629642358927523	32.58518588570543	30.11771234237221	13.667459412994834
6	21.347923888992945	40.42446947754914	27.359462929388418	10.868143704069501
7	85.64795513028798	5.273928016965238	7.221375669397183	1.8567411833496041
8	87.52585502346409	4.0828214458795165	6.6278646593245325	1.7634588713318615
9	82.93614448565384	5.629431139806819	8.534613993377139	2.8998103811621982
10	54.3401665540042	19.164234641628493	15.273902258535884	11.22169654583142
11	50.01952120967472	20.675960062659218	17.111766192944618	12.192752534721443
12	43.30602817162621	21.895952872377652	22.382897580437135	12.415121375559005
13	21.46755339446422	42.87991015459841	22.587934678095067	13.064601772842298
14	15.584217736739284	44.998835351015636	26.28500524552015	13.13194166672493
15	14.808005867034252	23.836519344396457	48.93030762556222	12.42516716300707
16	15.04292274274284	17.9867800381004	48.67588793905703	18.294409280099735
17	16.155834081861027	19.043170023664636	28.788650983217646	36.01234491125669
18	21.393185129143568	23.22306395045256	35.017664762873416	20.366086157530457
19	30.482635690806113	24.191396905382298	25.655211647814564	19.67075575599702
20	32.96011822383119	22.36733212955607	25.48689871086421	19.18565093574854
21	22.517282986152637	29.813137314139134	27.22886769256358	20.44071200714465
22	22.02750485086421	25.243739137762333	24.934969165320176	27.793786846053276
23	19.043280416933296	31.604268098148445	24.343371638570964	25.009079846347287
24	19.6661192387133	23.527712574198077	40.1651924872229	16.640975699865727
25	16.95430859407917	25.29654391793806	37.111236305255055	20.637911182727724
26	16.21103071619105	35.65205808010651	28.964507460193108	19.172403743509335
27	17.63793730913464	36.93335595168749	28.424426792151923	17.00427994702595
28	15.11269128853599	29.964412889959625	38.651148807550314	16.271747013954077
29	15.101541568401325	25.224530709015486	38.371706646815504	21.302221075767683
30	18.425262101218117	33.01906822929565	30.709935430977158	17.845734238509074
31	29.521073523020856	27.423895800529667	25.217023966746606	17.83800670970287
32	30.812417182049906	26.934927215878083	26.496518748272806	15.756136853799202
33	29.587824652803967	28.59082624577884	23.678583374700143	18.142765726717048
34	20.090434165686315	28.1600349137111	27.69332897157263	24.05620194902996
35	20.6347465756928	27.09370953396746	31.107314400397122	21.16422948994262
36	31.6834200717777	25.567448999229825	25.482446182361585	17.26668474663089
37	20.94189744686128	34.85862486049051	27.92103348706209	16.27844420558612
38	20.468015942259903	34.90318694327294	23.84362131134692	20.78517580312023
39	21.087211786174127	34.25429531008917	26.310027719749762	18.348465183986942
40	25.437368930992065	27.092164028206216	24.64073430658492	22.829732734216797
41	17.57449797741133	25.045141647442904	26.67815247297481	30.702207902170958
42	21.322864617007113	25.8712880723385	24.96657843791317	27.83926887274121
43	22.258079591338838	26.32857378888465	26.233451589055907	25.179895030720605
44	19.126627334771634	31.313639419522755	28.743831316141666	20.815901929563942
45	17.208029123216182	39.81520902781432	22.69795996919292	20.27880187977658
46	21.704015775934046	35.15893134900207	25.01213406011355	18.124918814950338
47	21.84793180051053	28.404188026230916	26.57618589048914	23.171694282769415
48	23.1795690026005	24.668884590093235	32.04263976799751	20.108906639308763
49	21.84307449668949	24.54369862343274	33.00596822808132	20.60725865179645
50	19.523196753554757	35.58582211891048	25.767886377360256	19.123094750174513
51	18.421103954765254	35.2295830409445	24.30506517434593	22.04424782994432
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1916.0
1	3066.0
2	4216.0
3	11715.0
4	19214.0
5	14583.5
6	9953.0
7	10585.5
8	11218.0
9	12289.5
10	13361.0
11	14147.5
12	14934.0
13	15007.5
14	15081.0
15	14349.0
16	13617.0
17	13179.5
18	12742.0
19	12180.0
20	11618.0
21	10725.0
22	9832.0
23	9982.0
24	10132.0
25	13128.5
26	19675.5
27	23226.0
28	26897.5
29	30569.0
30	33535.5
31	36502.0
32	43093.0
33	49684.0
34	59419.5
35	69155.0
36	75207.0
37	81259.0
38	99425.0
39	117591.0
40	168610.0
41	219629.0
42	261129.5
43	302630.0
44	305064.5
45	307499.0
46	292347.5
47	277196.0
48	245396.5
49	213597.0
50	200397.0
51	187197.0
52	167399.0
53	147601.0
54	132906.0
55	118211.0
56	104894.5
57	91578.0
58	84522.0
59	77466.0
60	66165.0
61	54864.0
62	51558.0
63	48252.0
64	41091.0
65	33930.0
66	28055.0
67	22180.0
68	19036.5
69	15893.0
70	13387.5
71	10882.0
72	9024.5
73	7167.0
74	6063.5
75	3646.5
76	2333.0
77	1901.0
78	1469.0
79	1074.5
80	680.0
81	415.0
82	150.0
83	143.5
84	137.0
85	105.5
86	74.0
87	48.0
88	22.0
89	16.0
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2717557.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.14090876703662
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.4511740956342	12.985550612786117
2	7.812846623389954	2.522128891179748
3	2.7609944795318606	1.3369488000124659
4	1.4084527708632926	0.9093483070873737
5	0.8383491652269308	0.67658586954246
6	0.5749096020004675	0.5567738061109727
7	0.4090084436613767	0.46212375818601437
8	0.30810503464183575	0.3978476203854824
9	0.24516454328273452	0.3561460673434937
>10	2.5033165750299164	9.497104787133335
>50	0.972965408643226	11.529987234725226
>100	1.6857321890151193	54.56447618022992
>500	0.02553094180778775	2.6585541677365603
>1k	0.0029901103018129802	0.6924269485051611
>5k	0.0	0.0
>10k+	4.6001696950968927E-4	0.8539969490356667
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	11846	0.43590622018231817	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11157	0.4105525661467266	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.035583430264756175	0.0
2	0.0	0.0	0.0	0.12489158461073678	0.0
3	0.0	0.0	0.0	0.18045619650296205	0.0
4	0.0	0.0	0.0	0.2841890712871892	0.0
5	0.0	0.0	0.0	0.47373431357649537	0.0
6	0.0	0.0	0.0	0.7067745037178613	0.0
7	0.0	0.0	0.0	0.8237177729850744	0.0
8	3.679775622001673E-5	0.0	0.0	1.130868644153554	0.0
9	3.679775622001673E-5	0.0	0.0	1.2645180947446548	0.0
10	7.359551244003346E-5	0.0	0.0	1.4897203628111573	0.0
11	7.359551244003346E-5	0.0	0.0	1.691850437727709	0.0
12	7.359551244003346E-5	0.0	0.0	1.8583234868670648	0.0
13	7.359551244003346E-5	0.0	0.0	1.9284968079786367	0.0
14	7.359551244003346E-5	0.0	0.0	1.9568310802680495	0.0
15	1.1039326866005018E-4	0.0	0.0	2.00168754510025	3.679775622001673E-5
16	1.1039326866005018E-4	0.0	0.0	2.1018878352873553	3.679775622001673E-5
17	1.1039326866005018E-4	0.0	0.0	2.2154089132261072	3.679775622001673E-5
18	1.1039326866005018E-4	0.0	0.0	2.3765462877135604	3.679775622001673E-5
19	1.1039326866005018E-4	0.0	0.0	2.452312867770575	3.679775622001673E-5
20	1.471910248800669E-4	0.0	0.0	2.531722425693371	3.679775622001673E-5
21	1.471910248800669E-4	0.0	0.0	2.6475985600302034	3.679775622001673E-5
22	1.471910248800669E-4	0.0	0.0	2.7709078411234795	3.679775622001673E-5
23	1.471910248800669E-4	0.0	0.0	2.917068528829386	3.679775622001673E-5
24	1.471910248800669E-4	0.0	0.0	3.0279769660765163	3.679775622001673E-5
25	1.471910248800669E-4	0.0	0.0	3.1168067495916367	3.679775622001673E-5
26	1.471910248800669E-4	0.0	0.0	3.209095522191439	3.679775622001673E-5
27	1.471910248800669E-4	0.0	0.0	3.296122215651778	3.679775622001673E-5
28	1.471910248800669E-4	0.0	0.0	3.3863503139032596	3.679775622001673E-5
29	1.8398878110008365E-4	0.0	0.0	3.484710716279364	3.679775622001673E-5
30	1.8398878110008365E-4	0.0	0.0	3.616373088034584	3.679775622001673E-5
31	1.8398878110008365E-4	0.0	0.0	3.7432885492374215	3.679775622001673E-5
32	1.8398878110008365E-4	0.0	0.0	3.853755413409912	3.679775622001673E-5
33	2.2078653732010036E-4	0.0	0.0	3.9654366035376625	3.679775622001673E-5
34	2.575842935401171E-4	0.0	0.0	4.089040266680699	3.679775622001673E-5
35	2.575842935401171E-4	0.0	0.0	4.243554045048549	3.679775622001673E-5
36	2.575842935401171E-4	0.0	0.0	4.37760827095807	3.679775622001673E-5
37	2.575842935401171E-4	0.0	0.0	4.510484968668551	3.679775622001673E-5
38	3.3117980598015053E-4	0.0	0.0	4.639461104219709	3.679775622001673E-5
39	3.3117980598015053E-4	0.0	0.0	4.770718700656508	3.679775622001673E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	35	1.2127384E-7	45.000004	1
CGTACTC	20	7.0349884E-4	45.0	36
CGTCGAA	25	3.8922168E-5	45.0	24
CGCAATA	20	7.0349884E-4	45.0	33
TCGTTGC	30	2.1666E-6	44.999996	38
TACGCTA	30	2.1666E-6	44.999996	10
TACGAGT	30	2.1666E-6	44.999996	41
TAATGCG	130	0.0	43.26923	1
CGTTTTT	9110	0.0	41.31998	1
CATAGCG	55	6.184564E-11	40.909092	1
CGTTAGG	255	0.0	40.588234	2
CCGGCAT	50	1.0822987E-9	40.5	44
TTAACGG	325	0.0	40.153843	2
AAATGCG	85	0.0	39.705883	1
GTTACCT	40	3.4605546E-7	39.375	27
TATTACG	115	0.0	39.130436	1
CATTGCG	75	0.0	39.0	1
GCGATGA	550	0.0	38.863636	9
GTTTACG	145	0.0	38.793106	1
CTTAACG	70	0.0	38.57143	1
>>END_MODULE