##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549006_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3450595
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.27149491609418	31.0	31.0	33.0	30.0	34.0
2	31.62859419897148	31.0	31.0	34.0	30.0	34.0
3	31.71723050662277	31.0	31.0	34.0	30.0	34.0
4	35.44147980275866	37.0	35.0	37.0	33.0	37.0
5	35.295650460282936	37.0	35.0	37.0	33.0	37.0
6	35.35443133720416	37.0	35.0	37.0	33.0	37.0
7	35.80516461653715	37.0	35.0	37.0	35.0	37.0
8	35.82839858053466	37.0	35.0	37.0	35.0	37.0
9	37.46663343568283	39.0	37.0	39.0	35.0	39.0
10	36.86091210356475	39.0	37.0	39.0	32.0	39.0
11	36.51969182126561	39.0	35.0	39.0	32.0	39.0
12	35.850327842009854	37.0	35.0	39.0	31.0	39.0
13	35.563168381105285	37.0	35.0	39.0	30.0	39.0
14	36.56530134657936	38.0	35.0	41.0	31.0	41.0
15	36.751672102927174	38.0	35.0	41.0	31.0	41.0
16	36.876097600558744	38.0	35.0	41.0	32.0	41.0
17	36.7934112812428	38.0	35.0	41.0	31.0	41.0
18	36.7064767090893	38.0	35.0	40.0	31.0	41.0
19	36.63335366799059	38.0	35.0	40.0	31.0	41.0
20	36.45891215862771	38.0	35.0	40.0	31.0	41.0
21	36.266199017850546	38.0	35.0	40.0	31.0	41.0
22	36.20099634990487	38.0	35.0	40.0	30.0	41.0
23	36.1336821620619	37.0	35.0	40.0	30.0	41.0
24	36.04856350861228	37.0	35.0	40.0	30.0	41.0
25	35.91455357699179	37.0	34.0	40.0	30.0	41.0
26	35.817022281664464	37.0	34.0	40.0	30.0	41.0
27	35.76151996974435	37.0	34.0	40.0	30.0	41.0
28	35.718081374371664	37.0	34.0	40.0	30.0	41.0
29	35.68286657808291	37.0	34.0	40.0	30.0	41.0
30	35.568675257455595	37.0	34.0	40.0	29.0	41.0
31	35.39836984635983	37.0	34.0	40.0	29.0	41.0
32	35.193298257257084	37.0	34.0	40.0	27.0	41.0
33	34.976850369284136	37.0	34.0	40.0	26.0	41.0
34	34.7554013148457	36.0	34.0	40.0	25.0	41.0
35	34.589994479213004	36.0	33.0	40.0	24.0	41.0
36	34.4037816666401	36.0	33.0	40.0	23.0	41.0
37	34.31999901466269	36.0	33.0	40.0	23.0	41.0
38	34.26320533125446	36.0	33.0	40.0	23.0	41.0
39	34.17510342419206	36.0	33.0	40.0	23.0	41.0
40	34.01855766903969	36.0	33.0	40.0	23.0	41.0
41	33.96261746162618	36.0	33.0	40.0	23.0	41.0
42	33.8535672833236	35.0	33.0	40.0	23.0	41.0
43	33.77086966160908	35.0	33.0	40.0	23.0	41.0
44	33.68762198983074	35.0	33.0	40.0	23.0	41.0
45	33.64044403936133	35.0	33.0	40.0	23.0	41.0
46	33.60898540686461	35.0	33.0	40.0	23.0	41.0
47	33.55625681947606	35.0	33.0	40.0	23.0	41.0
48	33.5040382890487	35.0	33.0	40.0	23.0	41.0
49	33.430875544652444	35.0	32.0	39.0	22.0	41.0
50	33.298711671465355	35.0	32.0	39.0	22.0	41.0
51	33.13699985075038	35.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	4.0
13	14.0
14	31.0
15	156.0
16	455.0
17	1215.0
18	2714.0
19	5213.0
20	8636.0
21	13455.0
22	19581.0
23	27495.0
24	37756.0
25	51116.0
26	65037.0
27	74394.0
28	80074.0
29	89338.0
30	103375.0
31	123331.0
32	149681.0
33	188995.0
34	308814.0
35	451012.0
36	256581.0
37	307670.0
38	419919.0
39	664150.0
40	379.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.935622696955164	24.15479069551773	27.867744548403973	16.041842059123137
2	31.854100524692118	26.12595798695587	28.85328472335931	13.166656764992704
3	28.38814755136433	24.537362396919953	32.911918089488914	14.162571962226805
4	25.860989191719106	27.917822868229976	31.748524529827467	14.472663410223454
5	23.398979016662345	32.08817609716585	30.268026238952995	14.244818647218812
6	22.195389490797965	39.17762009160739	28.17293829035282	10.454052127241823
7	83.52826106801871	5.347831316048392	8.907594197522457	2.216313418410448
8	84.73605276771107	4.407471754871262	8.655347845806304	2.2011276316113597
9	79.36132753916354	6.486997170053281	10.614285362379531	3.5373899284036523
10	47.661838030832364	22.023795896070098	17.45461290009404	12.859753173003497
11	40.84385446567911	22.45711246900897	21.582017014456927	15.117016050854998
12	35.40673420091318	21.711125182758337	27.245706899824523	15.636433716503964
13	21.85831718877469	34.42516435571257	27.672299994638607	16.044218460874138
14	17.496895462956388	35.96455104119724	30.36638608703716	16.172167408809205
15	17.963220835826867	22.975052128690848	43.116390071857175	15.945336963625115
16	18.957918851676308	19.901060541732658	41.4677468668447	19.67327373974633
17	18.89219105690468	20.476062823947753	30.831900005651196	29.79984611349637
18	22.39787630828886	22.819368833491037	34.23942827251532	20.543326585704786
19	29.13923540722687	23.748657840169592	27.186007050957876	19.926099701645658
20	30.51392585916342	22.177595458174604	27.729855285827515	19.57862339683446
21	23.61621691331495	26.56973652370099	28.958802757205643	20.85524380577842
22	23.446825837283136	24.27421937376018	27.079532660309308	25.19942212864738
23	21.850550412320192	27.592342769870125	27.253531637297336	23.303575180512347
24	20.84402834873406	23.207388870615066	37.2514595308925	18.697123249758373
25	19.18614036130001	25.133897197439865	34.607017050682565	21.072945390577566
26	18.81991366706322	30.97610701922422	29.424519539383788	20.779459774328775
27	19.60085144735908	30.81610562815978	29.73675554505817	19.846287379422968
28	17.883727299204917	27.777296379320088	35.690105619465626	18.64887070200936
29	19.147596284119114	24.1477484317922	34.74655820228106	21.958097081807633
30	19.73552387341893	29.066349426693076	31.329234523321343	19.86889217656665
31	27.087647202873704	26.289697863701768	27.00821162727008	19.614443306154445
32	28.488159288470538	25.258455425803376	27.890117501474382	18.363267784251704
33	27.02415090730729	27.043220082333626	26.10581653309067	19.826812477268412
34	20.294210128977756	26.90698270877921	29.361197126872323	23.437610035370714
35	21.872198852661644	26.172413743137053	30.398293627620742	21.55709377658056
36	28.041395759282096	27.30491408003547	26.08605182584453	18.567638334837905
37	22.298589083911615	32.00317626380378	28.195919834115568	17.50231481816904
38	21.865098627917796	31.92374068820015	25.603352465299462	20.607808218582594
39	22.271115561229298	31.13651413741688	27.008936139999047	19.58343416135478
40	23.963809140162784	26.254515525583265	27.046292016304434	22.735383317949513
41	19.332202127459176	25.911386297145857	27.869715223026752	26.886696352368215
42	21.86837342545271	26.15024365363075	25.88376787191774	26.097615048998797
43	22.216835067575303	25.95798695587283	27.776108178444588	24.049069798107283
44	21.00507303812821	28.869861574598005	28.281354375115015	21.843711012158774
45	19.511794342714808	33.85526264310938	25.285291377284207	21.34765163689161
46	21.211182419263924	32.015724824269434	26.470739104415326	20.302353652051313
47	21.810702212227167	27.292829207716352	28.09912493352596	22.79734364653053
48	22.94328949065306	24.44830529227568	30.736583111028676	21.871822106042583
49	21.692751539951804	25.566315374594815	31.38826782047734	21.35266526497604
50	20.238277746301726	31.88621092883981	27.161344637663937	20.714166687194528
51	19.841795400503393	31.57177240446937	26.27709134221779	22.30934085280944
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3350.0
1	5481.0
2	7612.0
3	22551.5
4	37491.0
5	27960.0
6	18429.0
7	18260.0
8	18091.0
9	19379.0
10	20667.0
11	21201.5
12	21736.0
13	21288.0
14	20840.0
15	20425.5
16	20011.0
17	19651.5
18	19292.0
19	18925.5
20	18559.0
21	18786.5
22	19014.0
23	19486.0
24	19958.0
25	22506.0
26	29336.0
27	33618.0
28	42033.5
29	50449.0
30	54758.5
31	59068.0
32	67693.0
33	76318.0
34	82731.5
35	89145.0
36	96239.5
37	103334.0
38	120012.0
39	136690.0
40	175597.5
41	214505.0
42	247292.5
43	280080.0
44	290151.5
45	300223.0
46	289194.5
47	278166.0
48	264454.0
49	250742.0
50	242777.5
51	234813.0
52	211966.0
53	189119.0
54	179346.0
55	169573.0
56	155517.0
57	141461.0
58	133933.0
59	126405.0
60	121715.0
61	117025.0
62	104381.5
63	91738.0
64	80585.0
65	69432.0
66	60904.5
67	52377.0
68	45520.5
69	38664.0
70	33921.5
71	29179.0
72	25338.5
73	21498.0
74	16904.0
75	9769.5
76	7229.0
77	5457.5
78	3686.0
79	2869.5
80	2053.0
81	1413.5
82	774.0
83	592.0
84	410.0
85	328.0
86	246.0
87	183.5
88	121.0
89	70.0
90	19.0
91	15.5
92	12.0
93	9.0
94	6.0
95	3.0
96	0.0
97	1.0
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3450595.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.78379751062978
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.31924022056238	13.648456615801797
2	6.524200851972194	2.1900173203635913
3	2.1852308908902622	1.1002941836002584
4	1.3027444994181585	0.8745999954528451
5	0.8178017709600608	0.6862909663814046
6	0.5834727633149774	0.5875733227467718
7	0.43610346319524396	0.5123630553967353
8	0.34914228787816076	0.4687946769716048
9	0.2749504997247012	0.41532421615432674
>10	3.097042780705659	12.682332802628258
>50	1.4835483619323697	18.12074062219303
>100	1.615358382395025	46.12399557137972
>500	0.008721271133426479	0.9384168484640023
>1k	0.001918679649353825	0.6671820301511344
>5k	3.488508453370591E-4	0.3785284620383575
>10k+	1.7442542266852954E-4	0.6050893102761647
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	20669	0.5989981437983883	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7597	0.2201649280776214	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5333	0.1545530553426293	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3917	0.1135166543741007	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3507	0.10163464561908889	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.016431948692906585	0.0
2	0.0	0.0	0.0	0.05280248768690617	0.0
3	0.0	0.0	0.0	0.08499983336207234	0.0
4	0.0	0.0	0.0	0.157509067276803	0.0
5	0.0	0.0	0.0	0.2693448521197069	0.0
6	0.0	0.0	0.0	0.4763526290393396	0.0
7	0.0	0.0	0.0	0.6043305574835645	0.0
8	0.0	0.0	0.0	0.8964251092927452	0.0
9	2.8980509158565407E-5	0.0	0.0	1.0340245667776138	0.0
10	2.8980509158565407E-5	0.0	0.0	1.2266869916637566	0.0
11	2.8980509158565407E-5	0.0	0.0	1.387036148838099	0.0
12	8.694152747569623E-5	0.0	0.0	1.5262874953450056	0.0
13	8.694152747569623E-5	0.0	0.0	1.5890882586916169	0.0
14	8.694152747569623E-5	0.0	0.0	1.6147939703152645	0.0
15	8.694152747569623E-5	0.0	0.0	1.64336875234561	0.0
16	8.694152747569623E-5	0.0	0.0	1.6997938036773368	0.0
17	8.694152747569623E-5	0.0	0.0	1.7611165610568613	0.0
18	8.694152747569623E-5	0.0	0.0	1.8586939353937508	0.0
19	1.1592203663426163E-4	0.0	0.0	1.909177982347972	0.0
20	1.1592203663426163E-4	0.0	0.0	1.9645017743316733	0.0
21	1.4490254579282703E-4	0.0	0.0	2.0328378149275705	0.0
22	1.4490254579282703E-4	0.0	0.0	2.107085879391815	0.0
23	1.4490254579282703E-4	0.0	0.0	2.192897167010327	0.0
24	1.7388305495139245E-4	0.0	0.0	2.2629720381557386	0.0
25	2.0286356410995784E-4	0.0	0.0	2.321425725128565	0.0
26	2.0286356410995784E-4	0.0	0.0	2.3786622307167313	0.0
27	2.0286356410995784E-4	0.0	0.0	2.435695872740788	0.0
28	2.0286356410995784E-4	0.0	0.0	2.495134897025006	0.0
29	2.0286356410995784E-4	0.0	0.0	2.563094191001842	0.0
30	2.0286356410995784E-4	0.0	0.0	2.652759886338443	0.0
31	2.0286356410995784E-4	0.0	0.0	2.7337024484183163	0.0
32	2.0286356410995784E-4	0.0	0.0	2.8139784587875423	0.0
33	2.0286356410995784E-4	0.0	0.0	2.8906608860211067	2.8980509158565407E-5
34	2.0286356410995784E-4	0.0	0.0	2.9676620988554148	2.8980509158565407E-5
35	2.3184407326852326E-4	0.0	0.0	3.064862726573243	2.8980509158565407E-5
36	2.6082458242708865E-4	0.0	0.0	3.1502682870635352	2.8980509158565407E-5
37	2.6082458242708865E-4	0.0	0.0	3.2354130229714007	2.8980509158565407E-5
38	3.187856007442195E-4	0.0	0.0	3.327078373439943	2.8980509158565407E-5
39	3.187856007442195E-4	0.0	0.0	3.41683101030402	2.8980509158565407E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACGTA	35	1.2128658E-7	45.000004	17
CAGTACG	140	0.0	41.785717	1
CTATGCG	155	0.0	40.64516	1
TATTACG	145	0.0	40.344826	1
CGTTTTT	16870	0.0	39.678425	1
GTAGGGC	1600	0.0	39.375	4
GCGATCG	100	0.0	38.25	9
ACATACG	1095	0.0	37.80822	17
TATAGCG	210	0.0	37.500004	1
CGTCGAA	30	1.14039576E-4	37.499996	14
CGTAAGG	415	0.0	37.409637	2
CATACGA	1110	0.0	37.2973	18
GGCGATA	1715	0.0	37.259476	8
TTACGAG	145	0.0	37.24138	1
CGCATCG	55	2.752131E-9	36.81818	21
CGAATAT	440	0.0	36.81818	14
AACGTAG	270	0.0	36.666664	1
GCTACGA	430	0.0	36.627907	10
TTACGGG	1235	0.0	36.43725	3
TAGGGCG	1570	0.0	36.40127	5
>>END_MODULE