##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549005_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	115545
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.395715954822798	31.0	31.0	33.0	30.0	34.0
2	31.778908650309404	31.0	31.0	34.0	30.0	34.0
3	31.961218572850406	31.0	31.0	34.0	30.0	34.0
4	35.709619628716084	37.0	35.0	37.0	33.0	37.0
5	35.63898048379419	37.0	35.0	37.0	33.0	37.0
6	35.704998052706735	37.0	35.0	37.0	33.0	37.0
7	35.98345233458826	37.0	35.0	37.0	35.0	37.0
8	36.026630317192435	37.0	35.0	37.0	35.0	37.0
9	37.951733091003504	39.0	38.0	39.0	35.0	39.0
10	37.405313946947075	39.0	37.0	39.0	35.0	39.0
11	36.98483707646371	39.0	37.0	39.0	33.0	39.0
12	35.84966030550868	37.0	35.0	39.0	32.0	39.0
13	35.28603574364966	37.0	35.0	39.0	30.0	39.0
14	36.184629365182396	38.0	35.0	40.0	31.0	41.0
15	36.56638539097322	38.0	35.0	41.0	32.0	41.0
16	36.77544679562075	38.0	35.0	41.0	32.0	41.0
17	36.75557574970791	38.0	35.0	41.0	32.0	41.0
18	36.73597299753343	38.0	35.0	40.0	32.0	41.0
19	36.67153057250422	37.0	35.0	40.0	32.0	41.0
20	36.51617118871435	37.0	35.0	40.0	32.0	41.0
21	36.37372452291315	37.0	35.0	40.0	32.0	41.0
22	36.24014885975161	36.0	35.0	40.0	31.0	41.0
23	36.20675927127959	36.0	35.0	40.0	31.0	41.0
24	36.09910424509931	36.0	35.0	40.0	31.0	41.0
25	35.973681249729545	36.0	35.0	40.0	31.0	41.0
26	35.81833917521312	35.0	35.0	40.0	31.0	41.0
27	35.70411527976113	35.0	34.0	40.0	31.0	41.0
28	35.688251330650395	36.0	35.0	40.0	30.0	41.0
29	35.87628196806439	36.0	35.0	40.0	31.0	41.0
30	35.90043705915444	36.0	35.0	40.0	31.0	41.0
31	35.73659613137738	36.0	35.0	40.0	31.0	41.0
32	35.56709507118439	35.0	34.0	40.0	30.0	41.0
33	35.38930286901207	35.0	34.0	40.0	30.0	41.0
34	35.289748582803234	35.0	34.0	40.0	30.0	41.0
35	35.14999350902246	35.0	34.0	40.0	29.0	41.0
36	34.95441602838721	35.0	34.0	40.0	28.0	41.0
37	34.80621402916613	35.0	34.0	40.0	27.0	41.0
38	34.82065861785451	35.0	34.0	40.0	28.0	41.0
39	34.81572547492319	35.0	34.0	40.0	28.0	41.0
40	34.61114717209745	35.0	34.0	40.0	27.0	41.0
41	34.63311264009693	35.0	34.0	40.0	27.0	41.0
42	34.599567268163916	35.0	34.0	40.0	27.0	41.0
43	34.46320481197802	35.0	33.0	40.0	27.0	41.0
44	34.32258427452508	35.0	33.0	39.0	27.0	41.0
45	34.29124583495608	35.0	33.0	39.0	27.0	41.0
46	34.33687307975248	35.0	34.0	39.0	27.0	41.0
47	34.33713271885413	35.0	33.0	39.0	27.0	41.0
48	34.33658747674066	35.0	34.0	39.0	27.0	41.0
49	34.32216885196244	35.0	34.0	39.0	27.0	41.0
50	34.165589164394824	35.0	34.0	39.0	27.0	41.0
51	33.930131117746335	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	0.0
15	2.0
16	10.0
17	23.0
18	55.0
19	91.0
20	118.0
21	219.0
22	311.0
23	510.0
24	723.0
25	1099.0
26	1524.0
27	1774.0
28	2086.0
29	2414.0
30	3038.0
31	3759.0
32	4806.0
33	6774.0
34	13529.0
35	21349.0
36	7770.0
37	9631.0
38	13044.0
39	20870.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.46700419749881	24.143840062313384	30.240166169025056	13.148989571162751
2	29.90176987320957	26.411354883378767	29.829070924747935	13.857804318663725
3	30.042840451772037	25.480981435804235	30.702323769959754	13.773854342463975
4	26.891687221428878	28.229694058591893	29.841187416158206	15.037431303821021
5	23.151153230343155	33.02349733869921	29.415379289454325	14.409970141503312
6	22.669955428620884	41.47128824267601	25.22740057986066	10.631355748842443
7	88.91860314163313	4.39136267255182	5.049980527067376	1.6400536587476742
8	90.92042061534467	2.6431260547838504	4.8145744082392135	1.6218789216322644
9	87.11757323986326	3.8513133411225065	6.410489419706608	2.6206239993076292
10	60.47773594703363	17.76710372582111	10.96715565364144	10.78800467350383
11	53.6950971482972	18.565926695226967	14.969059673720194	12.769916482755637
12	46.3256739798347	20.341858150504134	19.668527413561815	13.663940456099354
13	21.73006188065256	43.270587217101564	20.925180665541564	14.074170236704314
14	16.56584014885975	45.04911506339521	24.946124886407894	13.43891990133714
15	14.591717512657407	21.280020771128132	50.11121208187286	14.0170496343416
16	15.734995023583883	15.485741486001125	49.3400839499762	19.43917954043879
17	17.201955947899087	15.699511013025228	27.1625773508157	39.93595568825998
18	22.53667402310788	22.511575576615172	32.5890345752737	22.362715825003246
19	31.757323986325677	22.95123112207365	23.37011553940023	21.92132935220044
20	34.929248344800726	22.032108702237224	21.770738673244193	21.267904279717857
21	24.11614522480419	26.841490328443463	26.59829503656584	22.444069410186508
22	24.484832749145355	24.59993941754295	23.142498593621532	27.772729239690165
23	21.676403132978493	31.72703275779999	21.127699164827558	25.46886494439396
24	22.613700289930332	23.27751092647886	36.52949067462893	17.579298108961876
25	18.777965294906746	23.69120256177247	34.91193907135748	22.618893071963303
26	18.28032368341339	33.35583538880956	25.735427755419966	22.62841317235709
27	20.760742567830714	31.720974512094855	26.831104764377518	20.687178155696913
28	16.549396339088666	28.153533255441605	37.967891297762776	17.329179107706956
29	17.35687394521615	23.62888917737678	36.76230040244061	22.251936474966463
30	19.496300142801505	28.821671210350946	30.65905058635164	21.022978060495912
31	30.948115452853862	25.467134017049638	22.11865506945346	21.46609546064304
32	33.192262754770866	25.111428447790907	23.905837552468732	17.790471244969492
33	29.509714829720025	25.863516378899998	24.199229737331777	20.427539054048207
34	20.331472586438185	25.93534986368947	28.49106408758492	25.24211346228742
35	20.983166731576443	24.173265827166905	30.770695400060582	24.07287204119607
36	33.07975247738976	22.308191613657016	24.5159894413432	20.096066467610022
37	22.9408455580077	31.099571595482278	27.20411960707949	18.755463239430526
38	22.262322039032412	32.484313470942055	21.61322428491064	23.64014020511489
39	21.7889134103596	31.739149249210264	24.828421826993814	21.643515513436324
40	24.635423428101603	25.372798476783938	23.670431433640573	26.321346661473882
41	18.031935609502792	22.85170279977498	25.757064347224023	33.359297243498204
42	23.872949932926566	26.278938941537927	21.77420052793284	28.073910597602662
43	23.61763814963867	26.231338439568997	24.413864728028038	25.73715868276429
44	21.847764940066643	29.551257085983817	26.122290016876544	22.478687957073003
45	18.370331905318274	34.89030247955342	21.618417066943614	25.12094854818469
46	22.773811069280367	31.570383833138603	25.14691245834956	20.50889263923147
47	23.2627980440521	25.816781340603228	26.93236401402051	23.98805660132416
48	25.427322688130165	20.889696654982906	29.569431823099222	24.1135488337877
49	21.10433164567917	21.364836211000043	31.93041672075815	25.60041542256264
50	19.984421653901077	31.878488900428405	25.56925873036479	22.567830715305725
51	19.33965121814012	34.08715219178675	21.420225886018436	25.152970704054695
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	26.0
1	78.0
2	130.0
3	291.5
4	453.0
5	370.0
6	287.0
7	284.0
8	281.0
9	321.5
10	362.0
11	373.5
12	385.0
13	360.0
14	335.0
15	338.5
16	342.0
17	313.5
18	285.0
19	314.0
20	343.0
21	284.0
22	225.0
23	241.0
24	257.0
25	298.0
26	403.0
27	467.0
28	599.0
29	731.0
30	903.5
31	1076.0
32	1280.5
33	1485.0
34	1616.0
35	1747.0
36	1911.0
37	2075.0
38	2837.0
39	3599.0
40	5517.0
41	7435.0
42	9449.0
43	11463.0
44	11992.5
45	12522.0
46	11765.5
47	11009.0
48	10139.5
49	9270.0
50	8711.5
51	8153.0
52	7811.0
53	7469.0
54	6809.5
55	6150.0
56	5932.5
57	5715.0
58	5309.5
59	4904.0
60	4598.0
61	4292.0
62	3945.0
63	3598.0
64	3179.5
65	2761.0
66	2339.0
67	1917.0
68	1574.5
69	1232.0
70	1115.5
71	999.0
72	773.0
73	547.0
74	447.5
75	311.0
76	274.0
77	206.0
78	138.0
79	97.5
80	57.0
81	44.5
82	32.0
83	24.0
84	16.0
85	10.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	3.0
92	6.0
93	3.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	115545.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.43428967069107
#Duplication Level	Percentage of deduplicated	Percentage of total
1	59.97434557461162	21.85122679475529
2	8.34718989025607	6.082478687957073
3	6.380350610480308	6.9739062702843055
4	5.406432609625161	7.879181271366134
5	4.572663784502827	8.330087844562724
6	3.9004228229369566	8.52654809814358
7	3.142667110076488	8.015059067895624
8	2.4846786070597178	7.2422000086546365
9	1.757803221055632	5.763988056601324
>10	4.0215687206043045	18.688822536674024
>50	0.00712622927454986	0.1575143883335497
>100	0.0047508195163665735	0.4889869747717339
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	335	0.2899303301743909	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	230	0.199056644597343	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.050196892985416935	0.0
2	0.0	0.0	0.0	0.16703448872733567	0.0
3	0.0	0.0	0.0	0.25358085594357177	0.0
4	0.0	0.0	0.0	0.42667359037604397	0.0
5	0.0	0.0	0.0	0.6932364014020511	0.0
6	0.0	0.0	0.0	1.0221125968237483	0.0
7	0.0	0.0	0.0	1.1969362586005452	0.0
8	0.0	0.0	0.0	1.661690250551733	0.0
9	0.0	0.0	0.0	1.8373793760006925	0.0
10	0.0	0.0	0.0	2.1013457960102127	0.0
11	0.0	0.0	0.0	2.440607555497858	0.0
12	0.0	0.0	0.0	2.6907265567527805	0.0
13	0.0	0.0	0.0	2.8015059067895627	0.0
14	0.0	0.0	0.0	2.840451772036869	0.0
15	0.0	0.0	0.0	2.899303301743909	0.0
16	0.0	0.0	0.0	3.037777489289887	0.0
17	0.0	0.0	0.0	3.190964559262625	0.0
18	0.0	0.0	0.0	3.3969449132372667	0.0
19	0.0	0.0	0.0	3.5016660175689127	0.0
20	0.0	0.0	0.0	3.612445367605695	0.0
21	0.0	0.0	0.0	3.778614392660868	0.0
22	0.0	0.0	0.0	3.933532389977931	0.0
23	0.0	0.0	0.0	4.136050889263923	0.0
24	0.0	0.0	0.0	4.290103422908824	0.0
25	0.0	0.0	0.0	4.396555454584794	0.0
26	0.0	0.0	0.0	4.509931195638063	0.0
27	0.0	0.0	0.0	4.615517763641871	0.0
28	0.0	0.0	0.0	4.745337314466226	0.0
29	0.0	0.0	0.0	4.845731100437059	0.0
30	0.0	0.0	0.0	4.989398070016011	0.0
31	0.0	0.0	0.0	5.157298022415509	0.0
32	0.0	0.0	0.0	5.2931758189449996	0.0
33	0.0	0.0	0.0	5.422995369769354	0.0
34	0.0	0.0	0.0	5.574451512397767	0.0
35	0.0	0.0	0.0	5.783028257388896	0.0
36	0.0	0.0	0.0	5.928426154312173	0.0
37	0.0	0.0	0.0	6.072958587563287	0.0
38	0.0	0.0	0.0	6.222683802847375	0.0
39	0.0	0.0	0.0	6.376736336492276	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATC	35	1.1992779E-7	45.000004	16
TTGATAG	20	7.006932E-4	45.000004	1
ACACGGG	20	7.006932E-4	45.000004	3
CCGATAG	20	7.006932E-4	45.000004	11
GAACTTT	20	7.006932E-4	45.000004	40
GGGTACC	35	1.1992779E-7	45.000004	7
GGCCGAT	20	7.006932E-4	45.000004	8
AGGGTAA	20	7.006932E-4	45.000004	6
CGACAAT	20	7.006932E-4	45.000004	20
AGAACTT	20	7.006932E-4	45.000004	39
ACGGGAC	20	7.006932E-4	45.000004	5
AGAAATC	20	7.006932E-4	45.000004	43
ATGAGAG	20	7.006932E-4	45.000004	1
TACGAAG	20	7.006932E-4	45.000004	1
TGTTATG	20	7.006932E-4	45.000004	1
TACCTGG	35	1.1992779E-7	45.000004	10
AGCTGCG	20	7.006932E-4	45.000004	1
GCGATGC	35	1.1992779E-7	45.000004	9
TCATCGA	20	7.006932E-4	45.000004	16
CGTAAGG	20	7.006932E-4	45.000004	2
>>END_MODULE