##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549003_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	845132
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.15001680210902	31.0	31.0	33.0	30.0	33.0
2	31.51167983226289	31.0	31.0	34.0	30.0	34.0
3	31.64783134468935	31.0	31.0	34.0	30.0	34.0
4	35.46699923798886	37.0	35.0	37.0	33.0	37.0
5	35.39033665746889	37.0	35.0	37.0	33.0	37.0
6	35.44450097736212	37.0	35.0	37.0	33.0	37.0
7	35.850300308117546	37.0	35.0	37.0	35.0	37.0
8	35.907240525740356	37.0	35.0	37.0	35.0	37.0
9	37.80817197786855	39.0	37.0	39.0	35.0	39.0
10	37.05621725363612	39.0	37.0	39.0	33.0	39.0
11	36.741171793282	39.0	35.0	39.0	32.0	39.0
12	36.17305344017266	38.0	35.0	39.0	32.0	39.0
13	35.9561050818097	38.0	35.0	39.0	31.0	39.0
14	36.971673064089394	39.0	35.0	41.0	31.0	41.0
15	37.175293326959576	39.0	35.0	41.0	32.0	41.0
16	37.23362504318852	39.0	35.0	41.0	32.0	41.0
17	37.157316253555656	39.0	35.0	41.0	32.0	41.0
18	37.07420497626406	39.0	35.0	41.0	32.0	41.0
19	37.012072670304754	38.0	35.0	41.0	32.0	41.0
20	36.87097518494152	38.0	35.0	41.0	31.0	41.0
21	36.77258463766607	38.0	35.0	41.0	31.0	41.0
22	36.65098706474255	38.0	35.0	41.0	31.0	41.0
23	36.54733816729221	38.0	35.0	40.0	31.0	41.0
24	36.48259916794063	38.0	35.0	40.0	31.0	41.0
25	36.36841700468093	38.0	35.0	40.0	30.0	41.0
26	36.206133479740444	38.0	35.0	40.0	30.0	41.0
27	36.07049431331437	38.0	35.0	40.0	30.0	41.0
28	36.00796680281897	38.0	35.0	40.0	30.0	41.0
29	36.095244293199165	38.0	35.0	40.0	30.0	41.0
30	36.07268687021672	38.0	35.0	40.0	30.0	41.0
31	35.96426238741404	38.0	35.0	40.0	30.0	41.0
32	35.79134265416527	38.0	34.0	40.0	29.0	41.0
33	35.55508725264219	38.0	34.0	40.0	28.0	41.0
34	35.333275748640446	38.0	34.0	40.0	27.0	41.0
35	35.19509378416626	38.0	34.0	40.0	26.0	41.0
36	35.04523553717053	38.0	34.0	40.0	25.0	41.0
37	34.924773881476504	38.0	34.0	40.0	25.0	41.0
38	34.874761575706515	37.0	34.0	40.0	25.0	41.0
39	34.79802326737125	37.0	34.0	40.0	24.0	41.0
40	34.68764761007748	37.0	34.0	40.0	24.0	41.0
41	34.629797475423956	37.0	34.0	40.0	24.0	41.0
42	34.567185954383454	37.0	33.0	40.0	24.0	41.0
43	34.463897947302904	37.0	33.0	40.0	24.0	41.0
44	34.35963612784749	37.0	33.0	40.0	23.0	41.0
45	34.34195368297497	37.0	33.0	40.0	23.0	41.0
46	34.32284542533001	37.0	33.0	40.0	23.0	41.0
47	34.30530733660541	36.0	33.0	40.0	23.0	41.0
48	34.227487540407886	36.0	33.0	40.0	23.0	41.0
49	34.192577017554655	36.0	33.0	40.0	24.0	41.0
50	34.08514527908066	36.0	33.0	40.0	23.0	41.0
51	33.85410444758925	36.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	9.0
15	28.0
16	60.0
17	188.0
18	431.0
19	896.0
20	1500.0
21	2421.0
22	3523.0
23	5184.0
24	7864.0
25	11107.0
26	14715.0
27	16486.0
28	17371.0
29	19356.0
30	22745.0
31	27436.0
32	33703.0
33	43307.0
34	69510.0
35	95931.0
36	63084.0
37	80003.0
38	115490.0
39	192700.0
40	78.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.446846173142184	23.830360227751406	27.99716493991471	14.7256286591917
2	31.970272099506346	25.277234798824328	29.484388237577086	13.268104864092237
3	28.643217864191627	24.754239574409677	32.7680173038058	13.834525257592897
4	25.031356048522596	27.556168740504443	32.54604014520809	14.866435065764874
5	22.200437328133358	32.52935636089983	31.3314369826252	13.938769328341607
6	20.539868328261147	41.12174192907144	28.296171485637743	10.04221825702967
7	88.73903721548824	3.10566870027404	6.79030021345778	1.3649938707799492
8	89.91849793878353	2.228645939332554	6.345044324436892	1.507811797447026
9	85.10090731388706	4.118883204043866	8.216822934168864	2.5633865479002096
10	44.04708376916269	22.454953782367724	19.098318369201497	14.39964407926809
11	36.99138122802118	25.573046577339397	20.54223482248927	16.89333737215015
12	31.242811773782087	23.156146022159852	28.10424880373717	17.496793400320897
13	21.87492604705537	32.99034943653773	28.398404036292558	16.73632048011435
14	19.550200442061122	34.29653592574888	29.384048882304775	16.769214749885226
15	19.007918289687293	25.250256764623753	39.44673731440769	16.29508763128127
16	19.644623561763133	21.815408717218137	38.89842060175216	19.641547119266576
17	20.092127620300733	23.17413137829357	29.03357108712012	27.700169914285578
18	23.416460387253117	24.397017270674876	31.78462062731029	20.401901714761717
19	27.03979969992853	26.939696994079032	25.78401953777635	20.23648376821609
20	28.399942257540832	24.977399980121447	26.79451257318383	19.828145189153883
21	24.521613191785423	26.886924172791943	28.27641125883294	20.315051376589693
22	24.05612377711411	25.364913409976193	26.693226620220273	23.88573619268943
23	21.969467491468787	28.902585631593645	26.746946039198612	22.381000837738956
24	21.83445899575451	25.505601491837957	33.48033206647009	19.17960744593744
25	20.38237813737972	26.782088478486198	31.741077133512874	21.094456250621203
26	20.19897483470038	30.4973660919241	28.35296734711264	20.95069172626288
27	20.763383708107135	29.448062551175436	29.23945608496661	20.549097655750817
28	19.478850641083287	27.85482031209326	33.49370275885897	19.172626287964484
29	20.200513055948658	25.09690793864154	32.86102052697093	21.84155847843887
30	21.087001793802624	28.018463387967795	30.23918157163615	20.65535324659343
31	25.04543668917992	26.713460145870705	27.009863547942807	21.231239617006576
32	25.782008017682443	26.217797929790848	27.04252116829087	20.957672884235834
33	24.757552666329047	26.851426759370135	26.635957459899757	21.755063114401064
34	19.991196641471394	26.92632630169015	29.355532626855922	23.726944429982535
35	21.609523719371648	26.40960228698002	29.077942853897383	22.90293113975095
36	25.963044826133668	26.939105370522004	26.103851232706845	20.993998570637487
37	22.039042421775534	29.68163553149094	28.257479305007976	20.021842741725553
38	22.67551104442856	30.105356323035927	25.73325823658316	21.485874395952347
39	22.130389098980988	29.79144086367573	26.78031360781511	21.29785642952817
40	23.29470425921631	27.028085553499338	25.852884519814655	23.824325667469697
41	19.821164031180928	25.577542916372824	27.569421108181917	27.03187194426433
42	22.073356588083282	26.87272520742322	25.95641864229493	25.097499562198568
43	21.588580245452782	27.55818026059834	27.14570031663693	23.707539177311947
44	21.135041626633473	29.708140266845888	26.847167069759514	22.30965103676112
45	20.391489140157987	31.027697448445924	25.109450358050577	23.471363053345513
46	22.334262576733575	30.30189366868134	26.19614450760355	21.167699246981535
47	22.460751693226623	26.612174192907144	27.73235423578802	23.194719878078217
48	22.937600280192918	24.885106705224747	28.77136352664436	23.40592948793798
49	21.359148630036493	25.19582739737698	29.374937879526513	24.07008609306002
50	20.32262415811968	29.322401707662234	27.75708410047188	22.597890033746207
51	19.99474638281357	29.884680736263686	26.036169497782595	24.08440338314015
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	230.0
1	455.0
2	680.0
3	3362.0
4	6044.0
5	4719.0
6	3394.0
7	3375.0
8	3356.0
9	3513.0
10	3670.0
11	3682.5
12	3695.0
13	3771.0
14	3847.0
15	3727.5
16	3608.0
17	3617.0
18	3626.0
19	3661.0
20	3696.0
21	3610.5
22	3525.0
23	3834.0
24	4143.0
25	4478.5
26	5664.0
27	6514.0
28	8301.0
29	10088.0
30	11840.5
31	13593.0
32	16223.5
33	18854.0
34	21575.0
35	24296.0
36	26419.5
37	28543.0
38	32586.0
39	36629.0
40	44347.5
41	52066.0
42	59155.0
43	66244.0
44	66749.0
45	67254.0
46	66069.5
47	64885.0
48	65860.5
49	66836.0
50	66101.5
51	65367.0
52	62736.0
53	60105.0
54	54579.0
55	49053.0
56	44084.5
57	39116.0
58	35858.5
59	32601.0
60	30028.0
61	27455.0
62	24300.0
63	21145.0
64	18105.0
65	15065.0
66	13021.0
67	10977.0
68	9117.0
69	7257.0
70	6005.0
71	4753.0
72	3932.5
73	3112.0
74	2604.0
75	1784.0
76	1472.0
77	1101.0
78	730.0
79	575.0
80	420.0
81	279.5
82	139.0
83	94.5
84	50.0
85	50.0
86	50.0
87	38.5
88	27.0
89	17.0
90	7.0
91	5.0
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	845132.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.76630500060501
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.15988684618596	21.479332006727162
2	6.461031492521818	3.8464206804983725
3	3.096288878095196	2.7649523734608805
4	2.1115858358256014	2.5141643209656923
5	1.7161843005880522	2.554223266427697
6	1.4110245163336703	2.5200591669911514
7	1.2571592064613086	2.619468906169211
8	1.0851050901856423	2.583965525773986
9	0.9693089597844673	2.596747152308726
>10	9.642841973644655	54.04946345780755
>50	0.07718526748213711	1.4385095339113525
>100	0.011197861966494742	0.454742315414479
>500	7.998472833210459E-4	0.21320510128464043
>1k	3.9992364166052295E-4	0.3647461922591503
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3064	0.36254691574807246	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	954	0.11288177468135156	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03171102265681574	0.0
2	0.0	0.0	0.0	0.10625559084261393	0.0
3	0.0	0.0	0.0	0.17346402692123833	0.0
4	0.0	0.0	0.0	0.28291438497181504	0.0
5	0.0	0.0	0.0	0.5119910262538869	0.0
6	0.0	0.0	0.0	0.913585096765949	0.0
7	0.0	0.0	0.0	1.1298826692161699	0.0
8	0.0	0.0	0.0	1.6195103250143172	0.0
9	0.0	0.0	0.0	1.8781681441478963	0.0
10	0.0	0.0	0.0	2.2029694769574455	0.0
11	0.0	0.0	0.0	2.482097471164268	0.0
12	0.0	0.0	0.0	2.708570968795407	0.0
13	0.0	0.0	0.0	2.8248841601075334	0.0
14	0.0	0.0	0.0	2.8817983462938335	0.0
15	0.0	0.0	0.0	2.9529114978488566	0.0
16	0.0	0.0	0.0	3.0901681630798503	0.0
17	0.0	0.0	0.0	3.2584259026992233	0.0
18	0.0	0.0	0.0	3.461589432183375	0.0
19	0.0	0.0	0.0	3.5717497385023877	0.0
20	0.0	0.0	0.0	3.69504408778747	0.0
21	0.0	0.0	0.0	3.8564389941452935	0.0
22	0.0	0.0	0.0	4.0400789462474505	0.0
23	0.0	0.0	0.0	4.242532527463165	0.0
24	0.0	0.0	0.0	4.412328488330817	0.0
25	0.0	0.0	0.0	4.550886725387277	0.0
26	0.0	0.0	0.0	4.686013545812962	0.0
27	0.0	0.0	0.0	4.818300573164902	0.0
28	0.0	0.0	0.0	4.971294425012897	0.0
29	0.0	0.0	0.0	5.127601368780262	0.0
30	0.0	0.0	0.0	5.323310441445833	0.0
31	0.0	0.0	0.0	5.49949593672941	0.0
32	0.0	0.0	0.0	5.667398702214565	0.0
33	0.0	0.0	0.0	5.845595717592045	0.0
34	0.0	0.0	0.0	6.025804253063427	0.0
35	0.0	0.0	0.0	6.236422239366158	0.0
36	0.0	0.0	0.0	6.429528168380798	0.0
37	0.0	0.0	0.0	6.634703217958851	0.0
38	0.0	0.0	0.0	6.842008112342214	0.0
39	0.0	0.0	0.0	7.046118239517614	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGTATG	60	0.0	45.000004	1
CTATGCG	35	1.2114106E-7	45.0	1
TCCAACG	20	7.0322264E-4	45.0	43
CGTTTTT	3470	0.0	42.47118	1
TAGGGCG	355	0.0	41.830982	5
CGTTAGG	185	0.0	41.351353	2
TGGGCGA	640	0.0	39.726562	6
TAGGGTA	540	0.0	39.583336	5
GTGTACG	40	3.456771E-7	39.375	1
CGTATGG	150	0.0	39.0	2
ATTTGCG	110	0.0	38.863636	1
GGGCGAT	1875	0.0	38.4	7
AGGGCGA	915	0.0	38.114754	6
ATAGGGC	510	0.0	37.941177	4
GACCGAT	505	0.0	37.871284	9
ACGGGTA	155	0.0	37.741936	5
TTGGGCG	335	0.0	37.611942	5
AAACCGT	30	1.1396658E-4	37.500004	17
TAGGCGA	30	1.1396658E-4	37.500004	5
TACAGCG	30	1.1396658E-4	37.500004	1
>>END_MODULE