##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3549002_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3200787
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.136054039209732	31.0	31.0	33.0	30.0	33.0
2	31.490940509318488	31.0	31.0	34.0	30.0	34.0
3	31.684525711957715	31.0	31.0	34.0	30.0	34.0
4	35.481856180995486	37.0	35.0	37.0	33.0	37.0
5	35.39243067408109	37.0	35.0	37.0	33.0	37.0
6	35.43990399861034	37.0	35.0	37.0	33.0	37.0
7	35.78732886630694	37.0	35.0	37.0	35.0	37.0
8	35.82759146422427	37.0	35.0	37.0	35.0	37.0
9	37.72076304983743	39.0	37.0	39.0	35.0	39.0
10	37.04172755013064	39.0	37.0	39.0	33.0	39.0
11	36.67447474636707	39.0	35.0	39.0	32.0	39.0
12	36.0269421239214	37.0	35.0	39.0	32.0	39.0
13	35.74056599205133	37.0	35.0	39.0	31.0	39.0
14	36.72729144426043	39.0	35.0	41.0	31.0	41.0
15	36.95865891732252	39.0	35.0	41.0	31.0	41.0
16	37.0331809020719	38.0	35.0	41.0	32.0	41.0
17	36.97521172136727	38.0	35.0	41.0	32.0	41.0
18	36.914866562504784	38.0	35.0	41.0	31.0	41.0
19	36.86577488598898	38.0	35.0	41.0	31.0	41.0
20	36.736488557345425	38.0	35.0	41.0	31.0	41.0
21	36.615951639393685	38.0	35.0	41.0	31.0	41.0
22	36.48486887756042	38.0	35.0	40.0	31.0	41.0
23	36.38829856532159	38.0	35.0	40.0	30.0	41.0
24	36.27891827853587	38.0	35.0	40.0	30.0	41.0
25	36.13951162635939	38.0	35.0	40.0	30.0	41.0
26	35.977115628125205	38.0	34.0	40.0	30.0	41.0
27	35.83682325628041	38.0	34.0	40.0	30.0	41.0
28	35.75544327067062	38.0	34.0	40.0	29.0	41.0
29	35.89906607343757	38.0	35.0	40.0	30.0	41.0
30	35.87543501020218	38.0	35.0	40.0	30.0	41.0
31	35.79159625429621	38.0	34.0	40.0	30.0	41.0
32	35.66074437318072	38.0	34.0	40.0	29.0	41.0
33	35.48432213702443	37.0	34.0	40.0	28.0	41.0
34	35.34996455559211	37.0	34.0	40.0	27.0	41.0
35	35.232818678656216	37.0	34.0	40.0	27.0	41.0
36	35.08367692070731	37.0	34.0	40.0	26.0	41.0
37	34.95732861949264	37.0	34.0	40.0	26.0	41.0
38	34.902394004974404	37.0	33.0	40.0	26.0	41.0
39	34.83753464382353	37.0	33.0	40.0	26.0	41.0
40	34.69679113293075	37.0	33.0	40.0	25.0	41.0
41	34.629820728464594	36.0	33.0	40.0	25.0	41.0
42	34.55883662361788	36.0	33.0	40.0	25.0	41.0
43	34.448706208816766	36.0	33.0	40.0	24.0	41.0
44	34.34047345231032	36.0	33.0	40.0	24.0	41.0
45	34.295887230234314	36.0	33.0	40.0	24.0	41.0
46	34.282507708260496	35.0	33.0	40.0	24.0	41.0
47	34.24809679619418	35.0	33.0	40.0	24.0	41.0
48	34.1710897976029	35.0	33.0	40.0	24.0	41.0
49	34.12927539383283	35.0	33.0	40.0	24.0	41.0
50	33.99635714591442	35.0	33.0	40.0	24.0	41.0
51	33.74064534753484	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	8.0
12	10.0
13	23.0
14	51.0
15	122.0
16	328.0
17	785.0
18	1853.0
19	3371.0
20	5790.0
21	8980.0
22	13649.0
23	19186.0
24	26722.0
25	36934.0
26	48140.0
27	57396.0
28	66712.0
29	76710.0
30	91916.0
31	110713.0
32	136659.0
33	174764.0
34	288440.0
35	391918.0
36	248567.0
37	310595.0
38	427935.0
39	652219.0
40	279.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.99504559347435	23.84835354554989	28.43572533879949	13.720875522176264
2	30.486595952807853	26.191777209792466	30.044767115087634	13.276859722312043
3	29.665985271747232	25.867107058357835	30.373186344483404	14.093721325411531
4	26.241390008144872	28.362524591608253	29.99984066418665	15.396244736060224
5	23.905214561293832	32.90578223418178	28.339686458361648	14.84931674616274
6	22.319135887517664	41.14853628185818	25.88029131585451	10.652036514769648
7	89.41041687559965	3.75126492328293	5.230932267595438	1.6073859335219745
8	90.418825120197	2.9238746595759104	4.798538609410748	1.85876161081634
9	85.55470888878266	4.769983132273406	6.727501705049415	2.947806273894514
10	49.32724358103179	23.174925416780308	14.03520446690142	13.462626535286478
11	39.79814964257228	23.58876113905736	19.758015762998287	16.855073455372068
12	34.99283145051514	22.08691175014145	25.447522749873702	17.47273404946971
13	23.1301239351447	32.54465229957507	26.2921900145183	18.033033750761923
14	20.026543471964864	33.828961439795904	27.576093004626674	18.56840208361256
15	19.306595534160817	23.329418671095578	39.58938848476953	17.774597309974077
16	20.555632099230596	19.6247360414798	38.457479363668995	21.36215249562061
17	21.073973369674395	20.834907165019104	27.54597541167219	30.54514405363431
18	24.767440007723103	23.344914860001616	29.727407665677223	22.16023746659806
19	29.730032020250018	24.653468037704478	24.095167844658206	21.5213320973873
20	31.30064574743649	23.44726468834071	24.079577928803133	21.172511635419664
21	26.036190474405203	25.702866201343603	25.377290022735032	22.88365330151616
22	25.204426286410186	24.36503897322752	24.686397439129813	25.744137301232477
23	24.024903875203194	27.7283680544816	24.673556847112913	23.573171223202294
24	23.434923973385295	24.15430954949517	32.43271108011873	19.978055397000798
25	21.99996438375937	25.20683194476858	29.805169791054514	22.988033880417536
26	21.80729301887317	29.952102404814816	25.380101831205888	22.860502745106125
27	22.020115677800494	27.73477272933188	27.497581063657154	22.747530529210472
28	19.99564482110181	26.785006312510017	32.48101170118474	20.738337165203433
29	21.241650881486336	23.995817278688023	31.345259775174046	23.417272064651602
30	22.19610364575962	26.764886260785236	28.56647443269421	22.472535660760933
31	27.626111953091538	25.70349104767046	23.858351086779596	22.812045912458405
32	28.644142831122473	25.740606919485735	24.686366196813474	20.92888405257832
33	26.432780438061016	26.44137207505529	24.357884482785014	22.76796300409868
34	21.892428330907368	25.7202994138629	27.824219481021384	24.563052774208344
35	22.827104708935646	25.462331607820204	28.19328496397917	23.51727871926498
36	27.718651694098984	25.298684354816487	24.69577013403266	22.28689381705187
37	23.415116344823943	30.01233759072378	25.837708038679235	20.734838025773037
38	23.44813947319831	29.244651393547898	23.984976194917063	23.32223293833673
39	22.833384414520555	29.462472823090067	25.271003662536746	22.43313909985263
40	24.791184168143648	26.031910277066235	25.235574875803984	23.941330678986137
41	21.020299070197424	24.396406258835718	25.65078526000012	28.932509410966738
42	22.752310603610926	26.447339357476768	23.77209105135706	27.028258987555247
43	22.772711836182786	26.533037031205136	25.664156971394846	25.030094161217225
44	22.542955841797657	28.115116688489422	25.96355208890813	23.378375380804783
45	21.22524866540635	29.581443563723546	23.908869912305942	25.284437858564157
46	22.741344550574595	28.81272637010835	25.51859901955363	22.927330059763428
47	23.260123213447194	25.273065655415373	27.406759650048567	24.06005148108887
48	24.38303454744099	23.0924769439516	28.207062825486357	24.31742568312106
49	21.656705054100758	24.39709358979526	28.650078871227606	25.296122484876378
50	21.091000432081238	28.716093885659994	26.784194012285106	23.408711669973666
51	20.824628442942313	29.493840108698265	24.728699535458	24.95283191290142
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	729.0
1	2145.5
2	3562.0
3	10043.5
4	16525.0
5	12346.0
6	8167.0
7	8174.0
8	8181.0
9	8547.5
10	8914.0
11	9091.5
12	9269.0
13	9155.0
14	9041.0
15	9200.5
16	9360.0
17	8752.5
18	8145.0
19	8312.0
20	8479.0
21	9166.0
22	9853.0
23	10626.0
24	11399.0
25	12309.5
26	17984.0
27	22748.0
28	26436.5
29	30125.0
30	36004.0
31	41883.0
32	50712.0
33	59541.0
34	67145.5
35	74750.0
36	79460.5
37	84171.0
38	94617.0
39	105063.0
40	135076.5
41	165090.0
42	200015.0
43	234940.0
44	239680.0
45	244420.0
46	239168.0
47	233916.0
48	233536.5
49	233157.0
50	232864.0
51	232571.0
52	228809.0
53	225047.0
54	210268.0
55	195489.0
56	184355.0
57	173221.0
58	166497.0
59	159773.0
60	150511.0
61	141249.0
62	130008.0
63	118767.0
64	107038.0
65	95309.0
66	80175.0
67	65041.0
68	56321.5
69	47602.0
70	41319.5
71	35037.0
72	29337.0
73	23637.0
74	19422.0
75	11502.5
76	7798.0
77	6559.5
78	5321.0
79	3984.5
80	2648.0
81	1983.5
82	1319.0
83	960.0
84	601.0
85	435.0
86	269.0
87	191.5
88	114.0
89	89.5
90	65.0
91	53.5
92	42.0
93	25.5
94	9.0
95	5.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3200787.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.068996974576663
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.65652043233553	14.39313426697175
2	7.191952005320319	2.599027180508668
3	2.2668678114320833	1.2288008287959458
4	1.1031367446348466	0.7973029800540556
5	0.6804418480608646	0.6147450846993558
6	0.48793723956990986	0.5289921903343193
7	0.3675925613770505	0.4649420215579168
8	0.30793842749472816	0.445131081180651
9	0.24278113816423164	0.39481304858764027
>10	4.0557329558379065	19.14193935696964
>50	2.647555646904199	35.14779415515331
>100	0.9862979546814721	23.078863610695706
>500	0.0036716639308769844	0.4052626919218703
>1k	0.0013987291165245654	0.5030084643576566
>5k	1.7484113956557068E-4	0.25624303821159106
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8111	0.253406427856649	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	4920	0.15371219640669623	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3442	0.10753605285200171	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.02465018759448848	0.0
2	0.0	0.0	0.0	0.07979287593957361	0.0
3	3.124231634282444E-5	0.0	0.0	0.12984306672077836	0.0
4	3.124231634282444E-5	0.0	0.0	0.24875132272156816	0.0
5	3.124231634282444E-5	0.0	0.0	0.4439220729151924	0.0
6	3.124231634282444E-5	0.0	0.0	0.7943983776489969	0.0
7	3.124231634282444E-5	0.0	0.0	0.9899440356387351	0.0
8	3.124231634282444E-5	0.0	0.0	1.4850097804071312	0.0
9	3.124231634282444E-5	0.0	0.0	1.7286686055648188	0.0
10	3.124231634282444E-5	0.0	0.0	2.0130986535498927	0.0
11	3.124231634282444E-5	0.0	0.0	2.2885621567445757	0.0
12	3.124231634282444E-5	0.0	0.0	2.526472395695184	0.0
13	3.124231634282444E-5	0.0	0.0	2.639913246335979	0.0
14	3.124231634282444E-5	0.0	0.0	2.6888387137288423	0.0
15	3.124231634282444E-5	0.0	0.0	2.7458871833708396	0.0
16	3.124231634282444E-5	0.0	0.0	2.863264565870831	0.0
17	3.124231634282444E-5	0.0	0.0	2.9964818027566347	0.0
18	3.124231634282444E-5	0.0	0.0	3.1595042094334924	0.0
19	3.124231634282444E-5	0.0	0.0	3.25748011348459	0.0
20	3.124231634282444E-5	0.0	0.0	3.36389144294825	0.0
21	3.124231634282444E-5	0.0	0.0	3.506418890104215	0.0
22	3.124231634282444E-5	0.0	0.0	3.6659421573506767	0.0
23	3.124231634282444E-5	0.0	0.0	3.8339633346423865	0.0
24	3.124231634282444E-5	0.0	0.0	3.968211567967503	0.0
25	3.124231634282444E-5	0.0	0.0	4.082370991884184	0.0
26	3.124231634282444E-5	0.0	0.0	4.190938041175499	0.0
27	3.124231634282444E-5	0.0	0.0	4.292131903809906	0.0
28	3.124231634282444E-5	0.0	0.0	4.410259101902126	0.0
29	3.124231634282444E-5	0.0	0.0	4.531510531628627	0.0
30	3.124231634282444E-5	0.0	0.0	4.685472666566066	0.0
31	3.124231634282444E-5	0.0	0.0	4.824875882087749	0.0
32	3.124231634282444E-5	0.0	0.0	4.9658724557429155	0.0
33	3.124231634282444E-5	0.0	0.0	5.103713555447457	0.0
34	3.124231634282444E-5	0.0	0.0	5.2454599446948516	0.0
35	3.124231634282444E-5	0.0	0.0	5.417917530907243	0.0
36	6.248463268564887E-5	0.0	0.0	5.571629727313939	0.0
37	6.248463268564887E-5	0.0	0.0	5.72540440835332	0.0
38	6.248463268564887E-5	0.0	0.0	5.884646494752697	0.0
39	6.248463268564887E-5	0.0	0.0	6.0448883352750435	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGAATC	20	7.035175E-4	45.000004	19
TAATGCG	105	0.0	40.714287	1
CTATGCG	85	0.0	39.705883	1
GTACGAG	360	0.0	38.75	1
CGTTTTT	7065	0.0	38.312103	1
GGGCGAT	7720	0.0	37.976036	7
GCGATAA	425	0.0	37.588238	9
CGTACGT	30	1.1403773E-4	37.499996	31
CGACTTA	30	1.1403773E-4	37.499996	22
AGGGCGA	4395	0.0	37.474403	6
TAGGGCG	1290	0.0	37.15116	5
TATGGGC	1305	0.0	36.551723	4
CATACGA	925	0.0	36.486485	18
ATACTAT	2005	0.0	36.471317	45
TGGGCGA	2175	0.0	36.413795	6
CGTTAGG	555	0.0	36.08108	2
TAGTTCG	75	1.8189894E-12	36.000004	1
GTATACG	175	0.0	36.000004	1
GTCGAAT	25	0.0021076696	36.0	38
GTATCGA	25	0.0021076696	36.0	26
>>END_MODULE