FastQCFastQC Report
Sat 18 Jun 2016
SRR3548999_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548999_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5303891
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC111320.20988364956972155No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT105830.1995327581204063No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATACG1400.041.7857131
TAATGCG1300.039.807691
CGTTTTT117950.039.029251
CTATGCG2550.038.823531
GCGATAT6300.038.571439
CATACGA7500.038.39999818
AGGGCGA62450.038.334676
GGGCGAT124750.038.0921867
TGGGCGA38050.037.904076
GGCGATA31800.037.58
CGAACTA601.5643309E-1037.49999622
GACCGAT31400.037.4761129
TAAGGGC29050.037.4096374
GCTACGA8550.037.10526310
CACGACC4450.036.9101127
AGCTACG8500.036.7941179
CGGTCTA2400.036.562531
ATAGGGC26600.036.372184
ATGGGCG20750.036.3253025
GGCGATT37450.036.2883838
Produced by FastQC (version 0.11.5)