##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548998_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3416425
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.142438367591854	31.0	31.0	33.0	30.0	34.0
2	31.49320942212986	31.0	31.0	34.0	30.0	34.0
3	31.680004097850823	31.0	31.0	34.0	30.0	34.0
4	35.459928726666035	37.0	35.0	37.0	33.0	37.0
5	35.36305494778899	37.0	35.0	37.0	33.0	37.0
6	35.42020240456032	37.0	35.0	37.0	33.0	37.0
7	35.813581741147544	37.0	35.0	37.0	35.0	37.0
8	35.859364394066894	37.0	35.0	37.0	35.0	37.0
9	37.756320129960415	39.0	37.0	39.0	35.0	39.0
10	37.07297130772665	39.0	37.0	39.0	33.0	39.0
11	36.6711492276283	39.0	35.0	39.0	32.0	39.0
12	35.789233189664635	37.0	35.0	39.0	31.0	39.0
13	35.36020869768837	37.0	35.0	39.0	30.0	39.0
14	36.28589797814968	38.0	35.0	41.0	30.0	41.0
15	36.60446695010135	38.0	35.0	41.0	31.0	41.0
16	36.74451978310661	38.0	35.0	41.0	32.0	41.0
17	36.68268467769671	38.0	35.0	41.0	32.0	41.0
18	36.6430569967144	38.0	35.0	40.0	31.0	41.0
19	36.61043020116057	38.0	35.0	40.0	31.0	41.0
20	36.468336053037895	38.0	35.0	40.0	31.0	41.0
21	36.33148510504402	38.0	35.0	40.0	31.0	41.0
22	36.2069798107671	38.0	35.0	40.0	30.0	41.0
23	36.13514829097668	37.0	35.0	40.0	30.0	41.0
24	36.04159113693407	37.0	34.0	40.0	30.0	41.0
25	35.92274585275544	37.0	34.0	40.0	30.0	41.0
26	35.738604535442754	37.0	34.0	40.0	30.0	41.0
27	35.61959592263843	37.0	34.0	40.0	30.0	41.0
28	35.56181446980396	37.0	34.0	40.0	29.0	41.0
29	35.71473718872798	37.0	34.0	40.0	30.0	41.0
30	35.723932180568866	37.0	34.0	40.0	30.0	41.0
31	35.60070014708357	37.0	34.0	40.0	30.0	41.0
32	35.471215085945104	37.0	34.0	40.0	29.0	41.0
33	35.29006197999371	36.0	34.0	40.0	29.0	41.0
34	35.14789582677799	36.0	34.0	40.0	27.0	41.0
35	35.04013610718807	36.0	34.0	40.0	27.0	41.0
36	34.8432446197414	36.0	34.0	40.0	26.0	41.0
37	34.733775218247146	36.0	33.0	40.0	26.0	41.0
38	34.69963455951763	36.0	33.0	40.0	26.0	41.0
39	34.63299940727515	36.0	33.0	40.0	26.0	41.0
40	34.46011956943296	36.0	33.0	40.0	25.0	41.0
41	34.41897919608948	36.0	33.0	40.0	24.0	41.0
42	34.33473177371082	35.0	33.0	40.0	24.0	41.0
43	34.230174817243174	35.0	33.0	40.0	24.0	41.0
44	34.10734495854585	35.0	33.0	40.0	24.0	41.0
45	34.075400162450514	35.0	33.0	40.0	24.0	41.0
46	34.08721075393137	35.0	33.0	40.0	24.0	41.0
47	34.06677945513219	35.0	33.0	40.0	24.0	41.0
48	34.00318929875528	35.0	33.0	39.0	24.0	41.0
49	33.98202419195504	35.0	33.0	39.0	24.0	41.0
50	33.838184359381515	35.0	33.0	39.0	24.0	41.0
51	33.59043707969588	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	3.0
11	4.0
12	3.0
13	19.0
14	30.0
15	115.0
16	324.0
17	846.0
18	1736.0
19	3355.0
20	5961.0
21	9453.0
22	14292.0
23	20723.0
24	29181.0
25	40646.0
26	53495.0
27	63980.0
28	74034.0
29	84732.0
30	101011.0
31	123028.0
32	152556.0
33	197003.0
34	341581.0
35	492385.0
36	245587.0
37	301781.0
38	411886.0
39	646326.0
40	348.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.18863285451898	24.29065470484498	28.63212275990253	12.888589680733514
2	29.211646677447916	26.154445070504988	30.829595264055264	13.804312987991835
3	29.50309168209459	25.632525227394133	30.64729944313134	14.217083647379935
4	26.270326437723647	28.213527298272318	30.426864339184966	15.089281924819073
5	23.3615255713209	33.585634105827	28.30953408899654	14.743306233855566
6	21.77717936146703	41.756602296259985	25.787306907073916	10.678911435199074
7	88.69037078232363	4.230796812457466	5.347548972976137	1.7312834322427684
8	89.98098889921482	3.222842591305239	4.7365886855411725	2.0595798239387664
9	85.72346824531492	4.447426769210505	6.823594839634999	3.005510145839584
10	52.65152901058855	21.901754026504314	12.927519263557667	12.519197699349466
11	44.82890741052416	21.153018140307488	19.200362952501518	14.817711496666838
12	40.446958443402096	20.972273648623926	23.43877005934566	15.141997848628316
13	22.757385278470917	37.46416209927044	24.044754385066263	15.733698237192387
14	17.771325288861895	39.41289505843096	25.98011078832405	16.83566886438309
15	16.81488690663486	23.271021608845505	43.824055847852655	16.090035636666983
16	17.982393876640053	18.417234389749517	42.552463466928145	21.047908266682278
17	18.6798480868159	19.73191274504782	26.82479492451905	34.76344424361723
18	22.980776689082884	23.34416824604667	32.23477758182896	21.440277483041484
19	29.46082527788551	24.992879984194005	23.48290976678838	22.063384971132106
20	31.638306124091702	23.238092450441616	23.435345426871656	21.68825599859502
21	24.557190630556796	27.48958340955824	25.632291064489927	22.32093489539504
22	24.330491668922924	24.683784950642853	23.419861404831074	27.565861975603152
23	22.270941115347185	29.799483378092596	23.422290844962205	24.507284661598018
24	22.80260798934559	23.910257066963272	34.52170031538816	18.765434628302977
25	20.095450653826735	25.59277607440526	31.454868759009784	22.85690451275822
26	19.784833561398248	31.14223201153252	25.748172431708582	23.324761995360646
27	20.522036924562954	30.286132433757512	26.698024982254843	22.49380565942469
28	17.32117637589	28.121384195467485	34.3990867646736	20.158352663968916
29	18.654090167353303	24.31366121018316	33.26114286132434	23.771105761139204
30	20.337575096775137	28.473799366296642	29.307507116357012	21.881118420571212
31	27.993501979408297	27.05679182186057	22.83050264530906	22.11920355342207
32	29.1621504935715	26.14282473638379	23.642755219271606	21.052269550773104
33	26.978435060040834	27.127713911471783	24.133970451568526	21.759880576918857
34	20.277541582209473	25.949289096058013	27.83667137431672	25.936497947415795
35	21.715594517661003	25.157994102021853	28.638650050857255	24.48776132945989
36	29.373921570062272	24.749555456361545	24.28242973283476	21.59409324074142
37	22.41383902763854	30.258471940698247	26.150757004763754	21.176932026899465
38	21.776389061665338	30.504167367935782	24.200092201643532	23.519351368755352
39	21.85978032592549	29.709886796871	25.45883489319976	22.971497984003747
40	24.10007537118479	25.3772583914472	24.640464813364847	25.88220142400316
41	19.47026496996129	23.64808242534228	25.823748509040882	31.057904095655548
42	22.442875227760013	26.70502059901798	22.95771164301865	27.894392530203355
43	22.80199331172205	26.599237507043185	24.970049101033975	25.628720080200797
44	21.264772386339523	28.24736383793	26.472965160950405	24.01489861478007
45	19.102541399269704	32.661451663654255	22.667203289988805	25.568803647087236
46	22.479082666822777	30.83940083566886	25.34740262116101	21.334113876347352
47	22.6951272163153	25.72780025904271	26.93950547721668	24.637567047425303
48	23.78120403638306	22.665037283124903	28.717358057033305	24.836400623458733
49	21.396957345763482	23.111088345273203	30.09414812267209	25.397806186291227
50	19.991570135448605	29.946040085762167	26.815223515809656	23.247166262979576
51	19.55307082696093	31.211514960814302	23.850896770747198	25.384517441477566
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	787.0
1	2084.5
2	3382.0
3	9195.0
4	15008.0
5	11498.0
6	7988.0
7	8225.0
8	8462.0
9	8980.0
10	9498.0
11	9547.0
12	9596.0
13	9774.5
14	9953.0
15	9844.5
16	9736.0
17	9142.5
18	8549.0
19	8206.0
20	7863.0
21	7867.5
22	7872.0
23	8375.5
24	8879.0
25	11455.0
26	15900.5
27	17770.0
28	23797.0
29	29824.0
30	33909.5
31	37995.0
32	45025.0
33	52055.0
34	65910.0
35	79765.0
36	86224.0
37	92683.0
38	107531.0
39	122379.0
40	166890.5
41	211402.0
42	253220.5
43	295039.0
44	294036.5
45	293034.0
46	285275.5
47	277517.0
48	275091.5
49	272666.0
50	268195.5
51	263725.0
52	251171.5
53	238618.0
54	213203.5
55	187789.0
56	175611.0
57	163433.0
58	158695.5
59	153958.0
60	144647.0
61	135336.0
62	126332.0
63	117328.0
64	99939.0
65	82550.0
66	69523.0
67	56496.0
68	49155.0
69	41814.0
70	35099.5
71	28385.0
72	23009.5
73	17634.0
74	14027.5
75	9313.5
76	8206.0
77	5876.5
78	3547.0
79	2785.5
80	2024.0
81	1402.5
82	781.0
83	524.0
84	267.0
85	198.0
86	129.0
87	115.0
88	101.0
89	96.0
90	91.0
91	68.5
92	46.0
93	26.0
94	6.0
95	6.5
96	7.0
97	3.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3416425.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.555970398633296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.00794533626181	14.046171199788832
2	8.537319629151254	2.997618613861008
3	2.7181998453969416	1.4316190807007494
4	1.3086662075283666	0.9189962080423882
5	0.7212942166077161	0.6331509957735227
6	0.48479730093757545	0.5106652238758436
7	0.3147109295358063	0.38675390341398896
8	0.27906155052109544	0.3919357056276049
9	0.21012500640269224	0.33200535531764674
>10	2.389502028617406	10.143887925498683
>50	1.2089134181877856	15.594753640436835
>100	1.8096987825796016	50.71821676360453
>500	0.007576873659300154	0.8701831565660759
>1k	0.0018521246722733712	0.523475559468328
>5k	3.367499404133402E-4	0.5005666680239803
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	9176	0.268584851123616	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7758	0.2270794763532055	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	4809	0.14076117579048275	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.049671806054574594	0.0
2	2.9270363025677427E-5	0.0	0.0	0.19458937339470353	0.0
3	2.9270363025677427E-5	0.0	0.0	0.29012783831051464	0.0
4	2.9270363025677427E-5	0.0	0.0	0.490717636125482	0.0
5	2.9270363025677427E-5	0.0	0.0	0.8384788192335555	0.0
6	2.9270363025677427E-5	0.0	0.0	1.2579816621175643	0.0
7	2.9270363025677427E-5	0.0	0.0	1.4857928975464119	0.0
8	2.9270363025677427E-5	0.0	0.0	2.0505352817638323	0.0
9	2.9270363025677427E-5	0.0	0.0	2.274951155081701	0.0
10	2.9270363025677427E-5	0.0	0.0	2.6193462464418213	0.0
11	2.9270363025677427E-5	0.0	0.0	2.98935290544941	0.0
12	2.9270363025677427E-5	0.0	0.0	3.2888179895651155	0.0
13	2.9270363025677427E-5	0.0	0.0	3.4181344534125584	0.0
14	2.9270363025677427E-5	0.0	0.0	3.4689770739881602	0.0
15	2.9270363025677427E-5	0.0	0.0	3.5502316017474405	0.0
16	2.9270363025677427E-5	0.0	0.0	3.7328493966646423	0.0
17	2.9270363025677427E-5	0.0	0.0	3.94576201731342	0.0
18	2.9270363025677427E-5	0.0	0.0	4.218678882164836	2.9270363025677427E-5
19	2.9270363025677427E-5	0.0	0.0	4.355108044227519	2.9270363025677427E-5
20	2.9270363025677427E-5	0.0	0.0	4.500201233745802	2.9270363025677427E-5
21	2.9270363025677427E-5	0.0	0.0	4.703922960404516	2.9270363025677427E-5
22	2.9270363025677427E-5	0.0	0.0	4.904220054589227	2.9270363025677427E-5
23	2.9270363025677427E-5	0.0	0.0	5.152022947964612	2.9270363025677427E-5
24	2.9270363025677427E-5	0.0	0.0	5.338680052979357	2.9270363025677427E-5
25	2.9270363025677427E-5	0.0	0.0	5.493432462296114	2.9270363025677427E-5
26	2.9270363025677427E-5	0.0	0.0	5.638379299999269	2.9270363025677427E-5
27	2.9270363025677427E-5	0.0	0.0	5.774486488068669	2.9270363025677427E-5
28	2.9270363025677427E-5	0.0	0.0	5.927833919960192	2.9270363025677427E-5
29	2.9270363025677427E-5	0.0	0.0	6.086508557922389	2.9270363025677427E-5
30	2.9270363025677427E-5	0.0	0.0	6.291108395471875	2.9270363025677427E-5
31	2.9270363025677427E-5	0.0	0.0	6.480663266426162	2.9270363025677427E-5
32	2.9270363025677427E-5	0.0	0.0	6.650753345968373	2.9270363025677427E-5
33	2.9270363025677427E-5	0.0	0.0	6.8305319156720845	2.9270363025677427E-5
34	2.9270363025677427E-5	0.0	0.0	7.015374258179237	2.9270363025677427E-5
35	2.9270363025677427E-5	0.0	0.0	7.25465947591415	2.9270363025677427E-5
36	2.9270363025677427E-5	0.0	0.0	7.449248849308853	2.9270363025677427E-5
37	2.9270363025677427E-5	0.0	0.0	7.642608867456478	2.9270363025677427E-5
38	2.9270363025677427E-5	0.0	0.0	7.832514982767074	2.9270363025677427E-5
39	2.9270363025677427E-5	0.0	0.0	8.024265130948287	2.9270363025677427E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGCGTA	20	7.035242E-4	45.000004	38
CGTGCTA	55	1.8189894E-12	45.0	13
GCGTAAG	350	0.0	41.14286	1
TATAGCG	280	0.0	40.98214	1
CGTTTTT	7145	0.0	40.59132	1
TACGTAG	145	0.0	38.793106	1
ACACCGT	35	6.2519102E-6	38.571426	43
CCGTAAC	35	6.2519102E-6	38.571426	34
ACCGTAT	35	6.2519102E-6	38.571426	20
TCGACGA	35	6.2519102E-6	38.571426	28
AATACGG	295	0.0	38.13559	2
GTAGCGT	65	9.094947E-12	38.076927	9
TGTAACG	155	0.0	37.741936	1
TACGGGA	795	0.0	37.641506	4
CGTTAGG	485	0.0	37.57732	2
AGGGTAT	1740	0.0	37.5	6
ACCTACG	30	1.14039336E-4	37.499996	35
ACGTCGA	30	1.14039336E-4	37.499996	34
GCGTAGC	60	1.5643309E-10	37.499996	9
GCGTAAT	60	1.5643309E-10	37.499996	9
>>END_MODULE