FastQCFastQC Report
Sat 18 Jun 2016
SRR3548997_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548997_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1603597
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39770.2480049538630965No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC30100.18770302014783016No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGAC302.1660398E-645.00000416
CGGTTAT207.034122E-445.044
TAATGCG253.8914975E-545.01
GCGTAAG1300.041.538461
TCTAACG451.9290383E-840.01
GCGATTC1650.039.5454529
TATTGCG1200.039.3750041
TAGGGCG6750.038.6666685
CATACGA4950.038.63636418
ATACGAA4950.038.63636419
CTAAGCG1050.038.571431
AGGGCGA20650.038.2445536
AGTACGG1650.038.1818162
CGTTTTT34600.038.041911
GCGATAT1600.037.968759
GGGCGAT39250.037.8343937
CCCATTA5150.037.5728233
CACGACC6500.037.38461327
TAGCATA6000.037.12500430
CGAAAAA5250.036.85714322
Produced by FastQC (version 0.11.5)