##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548997_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1603597
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.22563960895412	31.0	31.0	33.0	30.0	33.0
2	31.59118032772573	31.0	31.0	34.0	30.0	34.0
3	31.790560221801364	31.0	31.0	34.0	30.0	34.0
4	35.59174655477654	37.0	35.0	37.0	33.0	37.0
5	35.516582408173626	37.0	35.0	37.0	33.0	37.0
6	35.571795158010396	37.0	35.0	37.0	33.0	37.0
7	35.82689166916626	37.0	35.0	37.0	35.0	37.0
8	35.85533085931191	37.0	35.0	37.0	35.0	37.0
9	37.76781136407713	39.0	37.0	39.0	35.0	39.0
10	37.184046864642426	39.0	37.0	39.0	33.0	39.0
11	36.837444819365466	39.0	37.0	39.0	33.0	39.0
12	36.14612150060146	38.0	35.0	39.0	32.0	39.0
13	35.86169966643739	38.0	35.0	39.0	31.0	39.0
14	36.85155933816289	39.0	35.0	41.0	31.0	41.0
15	37.07942456864162	39.0	35.0	41.0	32.0	41.0
16	37.1646024531101	39.0	35.0	41.0	32.0	41.0
17	37.09093057669726	38.0	35.0	41.0	32.0	41.0
18	37.048191659126324	38.0	35.0	41.0	32.0	41.0
19	36.99777687286768	38.0	35.0	41.0	32.0	41.0
20	36.881067999004735	38.0	35.0	41.0	32.0	41.0
21	36.7612149436548	38.0	35.0	41.0	31.0	41.0
22	36.639512296418616	38.0	35.0	41.0	31.0	41.0
23	36.53830045828222	38.0	35.0	40.0	31.0	41.0
24	36.4228206962223	38.0	35.0	40.0	31.0	41.0
25	36.301916878118384	38.0	35.0	40.0	31.0	41.0
26	36.137960472612505	38.0	35.0	40.0	30.0	41.0
27	36.03162078751706	38.0	35.0	40.0	30.0	41.0
28	35.93734024196853	38.0	35.0	40.0	30.0	41.0
29	36.0678661783478	38.0	35.0	40.0	30.0	41.0
30	36.049663350579976	38.0	35.0	40.0	30.0	41.0
31	35.95040462161004	38.0	35.0	40.0	30.0	41.0
32	35.83634292156945	38.0	34.0	40.0	30.0	41.0
33	35.66864305682787	38.0	34.0	40.0	29.0	41.0
34	35.51007329148159	37.0	34.0	40.0	29.0	41.0
35	35.390010707178924	37.0	34.0	40.0	28.0	41.0
36	35.23333543278018	37.0	34.0	40.0	27.0	41.0
37	35.11888460754167	37.0	34.0	40.0	27.0	41.0
38	35.04484169027505	37.0	34.0	40.0	27.0	41.0
39	34.988333103641374	37.0	34.0	40.0	27.0	41.0
40	34.82327854192793	36.0	34.0	40.0	26.0	41.0
41	34.75789989629564	36.0	34.0	40.0	26.0	41.0
42	34.68474311189158	36.0	33.0	40.0	26.0	41.0
43	34.54605240593491	36.0	33.0	40.0	26.0	41.0
44	34.41983303785178	36.0	33.0	40.0	25.0	41.0
45	34.37690579366262	35.0	33.0	40.0	25.0	41.0
46	34.38685904251505	35.0	33.0	40.0	25.0	41.0
47	34.339024081486805	35.0	33.0	40.0	25.0	41.0
48	34.277585328483404	35.0	33.0	40.0	25.0	41.0
49	34.21341334512349	35.0	33.0	39.0	25.0	41.0
50	34.092199598777	35.0	33.0	39.0	24.0	41.0
51	33.85126936505868	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	2.0
12	5.0
13	8.0
14	23.0
15	47.0
16	116.0
17	278.0
18	740.0
19	1296.0
20	2363.0
21	3655.0
22	5548.0
23	8220.0
24	11429.0
25	16360.0
26	21450.0
27	26452.0
28	30336.0
29	35996.0
30	43587.0
31	53635.0
32	67365.0
33	88486.0
34	150867.0
35	207624.0
36	127495.0
37	158934.0
38	218672.0
39	322474.0
40	127.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.57315210741851	23.708388080047545	27.93507346297106	12.783386349562889
2	31.074640324221107	25.609863326010213	29.484652316012067	13.830844033756613
3	30.194743442398558	26.048938729618477	29.785974905166324	13.970342922816641
4	26.840097605570477	28.201599279619504	29.533791844210235	15.424511270599783
5	24.3468277877796	32.54234075020096	28.10774776954559	15.003083692473856
6	22.376257875264173	41.76323602501127	25.03540478062755	10.825101319097005
7	88.64982910294793	4.193073446757508	5.275702062301189	1.8813953879933671
8	89.89521681569622	3.176920385857544	5.013603791975166	1.9142590064710772
9	85.4718486003653	4.502814609905107	6.894687380931742	3.1306494087978463
10	50.38716086398266	23.185251656120585	13.063070085563892	13.364517394332864
11	41.259618220787395	23.208137705421	19.17196153397643	16.360282539815177
12	37.061244190404445	21.75097608688467	24.01095786534896	17.17682185736192
13	24.201591796442624	32.73815054530533	25.077560010401616	17.982697647850426
14	19.458317769364747	34.65665001867676	27.031105695508284	18.853926516450205
15	18.855048992982653	22.93581242668825	39.5384875377043	18.6706510426248
16	20.929385624941926	19.43767667312922	38.48784950333531	21.145088198593537
17	20.943541301212214	19.674207422438432	27.84028655578677	31.54196472056259
18	24.813092067395985	22.2406876540677	29.625897279678128	23.32032299885819
19	30.31023380562573	23.72310499458405	23.74436968889316	22.222291510897065
20	32.19611909974888	22.852811523094644	23.079489422841277	21.871579954315205
21	27.208394627827314	25.897653836967766	24.654261638054948	22.23968989714997
22	26.642728815282148	23.809598047389713	23.41897621409868	26.128696923229466
23	24.941989789205145	27.974671940643443	23.433007170754248	23.650331099397167
24	24.05660524433508	23.9680543178866	31.48995664122594	20.485383796552377
25	22.40138887762948	24.127009466842356	29.51832661198543	23.953275043542735
26	21.435622541074846	29.584178568555565	25.6973541357336	23.282844754635985
27	22.692796257413804	28.102135386883365	25.86404190080176	23.341026454901076
28	20.157932448115083	27.090596951727896	31.45066996259035	21.30080063756667
29	20.84345381040249	24.25017008637457	31.329879015737745	23.576497087485198
30	22.7996186074182	26.571389195664498	28.775122427891798	21.85386976902551
31	28.01664009099543	25.63399657145779	23.658313154739	22.691050182807775
32	29.328690437809502	25.358615662164496	24.378070051266	20.934623848760005
33	27.170978743412466	24.98408265917185	24.651767245760624	23.19317135165506
34	21.85517932498003	25.8458328370532	27.391483022230652	24.90750481573612
35	23.413052032399662	25.181638528882257	27.764955908498205	23.640353530219873
36	28.903209472205297	25.98763903898548	23.71106955176394	21.39808193704528
37	24.477721023424216	28.719310400306313	25.253664106380842	21.549304469888632
38	24.072631714826105	29.368101836059807	23.315084775040113	23.24418167407397
39	24.03808438154973	28.416304096353386	24.202402473938278	23.34320904815861
40	24.89104182659359	25.228470744208174	24.65164252614591	25.228844903052327
41	21.183377120311402	24.120461687069756	25.791143286000164	28.905017906618685
42	24.06676989293445	25.850509822605055	23.29014085209688	26.792579432363617
43	23.85231451543	26.312783074550527	24.912244161095337	24.922658248924137
44	22.811217531586802	28.148406363943064	25.29413562135624	23.7462404831139
45	21.128937008487796	29.999744324789834	23.38212156795005	25.48919709877232
46	23.57144594308919	28.705903041724323	24.86372823097075	22.858922784215736
47	23.950718291441053	25.28789964062043	27.143041549716045	23.61834051822247
48	24.96032357256842	23.075934913821865	27.726604626972986	24.23713688663673
49	23.19123819762696	24.222295252485505	28.287468734351584	24.298997815535948
50	21.34569969886449	28.495189252661362	26.343464099770703	23.815646948703446
51	21.3935920309155	29.272628970994585	24.357366595223116	24.976412402866803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	435.0
1	1174.0
2	1913.0
3	5013.5
4	8114.0
5	6077.5
6	4041.0
7	3970.0
8	3899.0
9	4122.5
10	4346.0
11	4411.0
12	4476.0
13	4629.5
14	4783.0
15	4573.5
16	4364.0
17	4231.0
18	4098.0
19	4162.0
20	4226.0
21	4226.5
22	4227.0
23	4581.0
24	4935.0
25	5516.5
26	8042.0
27	9986.0
28	12361.0
29	14736.0
30	17223.5
31	19711.0
32	22097.5
33	24484.0
34	28627.0
35	32770.0
36	34972.0
37	37174.0
38	41827.5
39	46481.0
40	64341.0
41	82201.0
42	97321.0
43	112441.0
44	114159.0
45	115877.0
46	115108.0
47	114339.0
48	113225.0
49	112111.0
50	114955.0
51	117799.0
52	114421.0
53	111043.0
54	106535.0
55	102027.0
56	96231.5
57	90436.0
58	88490.0
59	86544.0
60	82887.5
61	79231.0
62	72972.5
63	66714.0
64	58808.5
65	50903.0
66	44133.5
67	37364.0
68	31735.0
69	26106.0
70	22732.5
71	19359.0
72	16359.0
73	13359.0
74	11193.0
75	7168.0
76	5309.0
77	4247.0
78	3185.0
79	2353.0
80	1521.0
81	1185.0
82	849.0
83	591.5
84	334.0
85	235.0
86	136.0
87	94.5
88	53.0
89	36.0
90	19.0
91	13.0
92	7.0
93	6.0
94	5.0
95	3.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1603597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.763210713274386
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.87637477231075	15.39087204211103
2	6.175983831812705	2.441145396597805
3	2.0517837643689916	1.216495046198991
4	1.077455740582233	0.8517593934141511
5	0.7386644256589613	0.7299190345348928
6	0.5809711564872835	0.6889113230395734
7	0.4770038222947493	0.6598988935733895
8	0.39258288776092465	0.6206958666595922
9	0.3626652539505053	0.6450686848986303
>10	7.668443867207676	40.61012750919906
>50	2.458939460397351	31.48679221579338
>100	0.1353192084783995	3.7846961972955953
>500	0.0028588565170987977	0.37158287510576204
>1k	9.52952172366266E-4	0.5020355215781008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3977	0.2480049538630965	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	3010	0.18770302014783016	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045210860334610255	0.0
2	0.0	0.0	0.0	0.12415837644994347	0.0
3	0.0	0.0	0.0	0.17560521752036204	0.0
4	0.0	0.0	0.0	0.26827189125447354	0.0
5	0.0	0.0	0.0	0.44618442164708466	0.0
6	0.0	0.0	0.0	0.6911961047569932	0.0
7	0.0	0.0	0.0	0.8251449709621557	0.0
8	0.0	0.0	0.0	1.144988422901764	0.0
9	0.0	0.0	0.0	1.2900373348166654	0.0
10	0.0	0.0	0.0	1.5192719866649789	0.0
11	0.0	0.0	0.0	1.780684299110063	0.0
12	0.0	0.0	0.0	1.9909615695215195	0.0
13	0.0	0.0	0.0	2.0901136632208717	0.0
14	0.0	0.0	0.0	2.134264406830394	0.0
15	0.0	0.0	0.0	2.1938803826647217	0.0
16	6.235980735808312E-5	0.0	0.0	2.3189741562250368	0.0
17	6.235980735808312E-5	0.0	0.0	2.4631500308369247	0.0
18	6.235980735808312E-5	0.0	0.0	2.640438963155955	0.0
19	6.235980735808312E-5	0.0	0.0	2.7356623889917477	0.0
20	6.235980735808312E-5	0.0	0.0	2.8301374971392437	0.0
21	6.235980735808312E-5	0.0	0.0	2.9633380456561094	0.0
22	6.235980735808312E-5	0.0	0.0	3.1074515604606394	0.0
23	6.235980735808312E-5	0.0	0.0	3.27270504995956	0.0
24	6.235980735808312E-5	0.0	0.0	3.4052820004028446	0.0
25	6.235980735808312E-5	0.0	0.0	3.513850425013267	0.0
26	6.235980735808312E-5	0.0	0.0	3.6216081721280347	0.0
27	6.235980735808312E-5	0.0	0.0	3.719388350065509	0.0
28	6.235980735808312E-5	0.0	0.0	3.8287050923642285	0.0
29	6.235980735808312E-5	0.0	0.0	3.9433847780957434	0.0
30	6.235980735808312E-5	0.0	0.0	4.089992685194597	0.0
31	6.235980735808312E-5	0.0	0.0	4.226560663308799	0.0
32	6.235980735808312E-5	0.0	0.0	4.359012894137368	0.0
33	6.235980735808312E-5	0.0	0.0	4.4836077892388175	0.0
34	6.235980735808312E-5	0.0	0.0	4.6198639683162295	0.0
35	6.235980735808312E-5	0.0	0.0	4.7828101449429	0.0
36	6.235980735808312E-5	0.0	0.0	4.9286073745460985	0.0
37	6.235980735808312E-5	0.0	0.0	5.071286613781393	0.0
38	6.235980735808312E-5	0.0	0.0	5.212905736291599	0.0
39	6.235980735808312E-5	0.0	0.0	5.361758596455344	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTCGAC	30	2.1660398E-6	45.000004	16
CGGTTAT	20	7.034122E-4	45.0	44
TAATGCG	25	3.8914975E-5	45.0	1
GCGTAAG	130	0.0	41.53846	1
TCTAACG	45	1.9290383E-8	40.0	1
GCGATTC	165	0.0	39.545452	9
TATTGCG	120	0.0	39.375004	1
TAGGGCG	675	0.0	38.666668	5
CATACGA	495	0.0	38.636364	18
ATACGAA	495	0.0	38.636364	19
CTAAGCG	105	0.0	38.57143	1
AGGGCGA	2065	0.0	38.244553	6
AGTACGG	165	0.0	38.181816	2
CGTTTTT	3460	0.0	38.04191	1
GCGATAT	160	0.0	37.96875	9
GGGCGAT	3925	0.0	37.834393	7
CCCATTA	515	0.0	37.57282	33
CACGACC	650	0.0	37.384613	27
TAGCATA	600	0.0	37.125004	30
CGAAAAA	525	0.0	36.857143	22
>>END_MODULE