FastQCFastQC Report
Sat 18 Jun 2016
SRR3548995_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548995_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200843
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC4630.23052832311805738No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.21210597332244588No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATG207.0193695E-445.0000041
GGTACAT207.0193695E-445.0000048
CCCTCGT207.0193695E-445.00000414
GTACACT207.0193695E-445.00000418
GGATACA207.0193695E-445.0000048
GTTGTAG207.0193695E-445.0000041
GCTAGTT207.0193695E-445.0000049
TGAGCTT207.0193695E-445.00000430
CGATCTG207.0193695E-445.00000410
CGATAGT207.0193695E-445.00000412
TACGTAG207.0193695E-445.0000041
CTAGCGA207.0193695E-445.00000414
ACTATAG253.879275E-545.01
CGACAGG253.879275E-545.02
CAGGGCG551.8189894E-1245.05
AATGCGG253.879275E-545.02
CTTGCGG253.879275E-545.02
GTACTGG253.879275E-545.02
GCGATTG253.879275E-545.09
GCGATCT253.879275E-545.09