##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548995_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	200843
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.216372987856186	31.0	31.0	33.0	30.0	34.0
2	31.56936014698048	31.0	31.0	34.0	30.0	34.0
3	31.772538749172238	31.0	31.0	34.0	30.0	34.0
4	35.55146059359799	37.0	35.0	37.0	33.0	37.0
5	35.46820153054874	37.0	35.0	37.0	33.0	37.0
6	35.52456894190985	37.0	35.0	37.0	33.0	37.0
7	35.81177835423689	37.0	35.0	37.0	35.0	37.0
8	35.84429131211942	37.0	35.0	37.0	35.0	37.0
9	37.7578307434165	39.0	37.0	39.0	35.0	39.0
10	37.144436201411054	39.0	37.0	39.0	33.0	39.0
11	36.76901360764378	39.0	35.0	39.0	32.0	39.0
12	35.86080669976051	37.0	35.0	39.0	32.0	39.0
13	35.466892050009214	37.0	35.0	39.0	30.0	39.0
14	36.393376916297804	38.0	35.0	41.0	31.0	41.0
15	36.69338239321261	38.0	35.0	41.0	31.0	41.0
16	36.82441509039399	38.0	35.0	41.0	32.0	41.0
17	36.77306154558536	38.0	35.0	41.0	32.0	41.0
18	36.72316685172	38.0	35.0	40.0	32.0	41.0
19	36.67493016933625	38.0	35.0	40.0	31.0	41.0
20	36.534765961472395	38.0	35.0	40.0	31.0	41.0
21	36.40343950249697	38.0	35.0	40.0	31.0	41.0
22	36.28704510488292	38.0	35.0	40.0	31.0	41.0
23	36.2264654481361	37.0	35.0	40.0	31.0	41.0
24	36.110862713661916	37.0	35.0	40.0	31.0	41.0
25	35.99123195729998	37.0	35.0	40.0	30.0	41.0
26	35.7969209780774	37.0	34.0	40.0	30.0	41.0
27	35.63701000283804	37.0	34.0	40.0	30.0	41.0
28	35.57903934914336	36.0	34.0	40.0	29.0	41.0
29	35.729993079171294	37.0	34.0	40.0	30.0	41.0
30	35.72417261243857	37.0	34.0	40.0	30.0	41.0
31	35.59838281642875	36.0	34.0	40.0	30.0	41.0
32	35.480917930921166	36.0	34.0	40.0	29.0	41.0
33	35.30675701916422	36.0	34.0	40.0	29.0	41.0
34	35.17471856126427	36.0	34.0	40.0	28.0	41.0
35	35.047011845073015	36.0	34.0	40.0	27.0	41.0
36	34.88434747539122	36.0	34.0	40.0	26.0	41.0
37	34.757651498932	36.0	33.0	40.0	26.0	41.0
38	34.72270380346838	36.0	33.0	40.0	27.0	41.0
39	34.686750347286186	36.0	33.0	40.0	26.0	41.0
40	34.50252187031661	35.0	33.0	40.0	25.0	41.0
41	34.46946122095368	35.0	33.0	40.0	25.0	41.0
42	34.42171746090229	35.0	33.0	40.0	25.0	41.0
43	34.29957230274393	35.0	33.0	40.0	25.0	41.0
44	34.17856733866752	35.0	33.0	40.0	24.0	41.0
45	34.134393531265715	35.0	33.0	40.0	24.0	41.0
46	34.16866905991247	35.0	33.0	40.0	25.0	41.0
47	34.14811569235672	35.0	33.0	39.0	25.0	41.0
48	34.08335864331841	35.0	33.0	39.0	25.0	41.0
49	34.0665594519102	35.0	33.0	39.0	25.0	41.0
50	33.924667526376325	35.0	33.0	39.0	24.0	41.0
51	33.661317546541326	35.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	2.0
13	2.0
14	2.0
15	12.0
16	18.0
17	53.0
18	101.0
19	201.0
20	323.0
21	512.0
22	834.0
23	1172.0
24	1577.0
25	2326.0
26	2886.0
27	3519.0
28	4214.0
29	4702.0
30	5898.0
31	6978.0
32	8622.0
33	11699.0
34	20649.0
35	29886.0
36	14406.0
37	17743.0
38	25156.0
39	37324.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.91433607345041	23.792215810359334	28.62434837161365	12.66909974457661
2	29.9985560860971	26.17218424341401	30.2808661491812	13.54839352130769
3	29.968184104001633	25.56673620688797	30.368994687392643	14.096085001717759
4	26.72585053997401	28.39232634445811	29.331866184034293	15.549956931533588
5	24.70935008937329	32.062855065897246	28.138894559431993	15.088900285297472
6	22.263658678669408	42.127930771796876	24.90402951559178	10.704381033941935
7	88.75390230179792	4.448748524967263	4.990465189227407	1.8068839840074087
8	90.4218718103195	3.1034190885417963	4.68873697365604	1.7859721274826605
9	86.17875654117894	4.541856076636975	6.415956742331074	2.8634306398530196
10	54.5968741753509	21.523777278769984	11.86100586029884	12.01834268558028
11	46.214705018347665	21.203626713403008	17.17211951623905	15.409548752010277
12	40.988732492543924	21.060231125804734	22.443898965858907	15.507137415792435
13	23.5128931553502	37.02942099052493	23.137475540596387	16.320210313528477
14	18.118132073310996	39.50946759409091	25.939166413566817	16.433233919031284
15	17.55948676329272	22.322411037477032	44.30674706113731	15.811355138092939
16	19.1253864959197	17.455923283360637	42.72790189351882	20.69078832720085
17	19.824439985461282	18.243603212459483	26.796552531081492	35.135404270997746
18	24.370279272864874	22.765543235263365	30.80465836499156	22.0595191268802
19	31.753658330138467	23.687656527735594	22.7516019975802	21.80708314454574
20	32.819167210208974	23.05830922660984	22.316934122672933	21.805589440508257
21	25.194803901554945	26.284709947570988	25.40043715738164	23.12004899349243
22	25.171402538301063	23.99336795407358	24.138755147055164	26.6964743605702
23	23.39837584580991	29.301494201938826	23.019472921635305	24.280657030615956
24	23.788232599592718	23.199215307478976	33.81248039513451	19.2000716977938
25	21.791648202825094	23.667242572556674	31.499230742420696	23.041878482197536
26	20.47171173503682	31.219410186065733	24.442972869355668	23.865905209541783
27	21.862350193932574	28.849897681273433	27.17296594852696	22.114786176267035
28	18.593129957230275	27.484652191014874	34.25013567811674	19.672082173638113
29	19.736809348595667	24.641635506340773	33.09948566790976	22.522069477153796
30	21.940520705227467	27.524484298681056	28.85188928665674	21.683105709434734
31	29.055033035754295	25.96655098758732	23.023954033747753	21.954461942910633
32	30.72399834696753	26.075093480977678	23.147931468858758	20.05297670319603
33	28.762267044407817	25.958584566054082	23.754375308076458	21.52477308146164
34	21.287772040847827	26.732323257469766	27.37909710569948	24.600807595982932
35	22.46481082238365	25.549309659784008	28.30818101701329	23.677698500819048
36	30.00602460628451	25.522920888455165	23.793211613050993	20.677842892209338
37	23.489491792096313	30.40683518967552	25.609057821283294	20.494615196944878
38	23.37248497582689	31.22837241029062	22.2835747325025	23.115567881379985
39	23.01001279606459	30.269912319573	24.436002250514083	22.28407263384833
40	25.595116583600124	25.13455783870984	24.080002788247537	25.190322789442497
41	19.85979098101502	23.791717909013506	25.012074107636312	31.33641700233516
42	23.13100282310063	27.339762899379117	22.49767231120826	27.031561966311994
43	23.674711092744083	26.438561463431636	24.476830160871923	25.409897282952354
44	22.23776780868639	28.86881793241487	26.108452871148113	22.78496138775063
45	20.46623482023272	32.00360480574379	22.2890516473066	25.241108726716888
46	23.33364867085236	29.996066579367962	25.133064134672356	21.537220615107323
47	23.597038482795018	25.6220032562748	26.6491737327166	24.13178452821358
48	24.949836439407896	22.419501799913366	28.582026757218326	24.048635003460415
49	21.88973476795308	23.07274836563883	29.929347799027102	25.108169067380988
50	20.627554856280778	30.282359853218683	25.75942402772315	23.330661262777394
51	20.562827681323224	31.952320967123576	23.337133980273148	24.147717371280056
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	43.0
1	127.0
2	211.0
3	552.0
4	893.0
5	671.5
6	450.0
7	470.0
8	490.0
9	510.5
10	531.0
11	568.0
12	605.0
13	563.0
14	521.0
15	515.5
16	510.0
17	499.5
18	489.0
19	477.5
20	466.0
21	486.5
22	507.0
23	575.0
24	643.0
25	698.5
26	834.5
27	915.0
28	1126.5
29	1338.0
30	1701.0
31	2064.0
32	2489.5
33	2915.0
34	3394.5
35	3874.0
36	4212.5
37	4551.0
38	5542.0
39	6533.0
40	9123.0
41	11713.0
42	14323.5
43	16934.0
44	17039.0
45	17144.0
46	16590.0
47	16036.0
48	15343.0
49	14650.0
50	14801.5
51	14953.0
52	14483.0
53	14013.0
54	12937.0
55	11861.0
56	11035.0
57	10209.0
58	9829.5
59	9450.0
60	9439.5
61	9429.0
62	8462.0
63	7495.0
64	6451.5
65	5408.0
66	4729.0
67	4050.0
68	3373.5
69	2697.0
70	2308.0
71	1919.0
72	1710.5
73	1502.0
74	1267.0
75	785.5
76	539.0
77	394.5
78	250.0
79	186.5
80	123.0
81	98.5
82	74.0
83	53.0
84	32.0
85	22.0
86	12.0
87	8.5
88	5.0
89	4.5
90	4.0
91	3.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	200843.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.678973128264364
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.16699410609037	20.010655088800704
2	6.763064833005894	4.284938982190069
3	4.543811394891946	4.3182983723605
4	3.663654223968566	4.642432148494097
5	3.311591355599214	5.245390678291003
6	2.8275049115913555	5.374347126860284
7	2.6153241650294694	5.79955487619683
8	2.3544204322200395	5.966849728394815
9	2.0589390962671907	5.870256867304312
>10	8.666404715127701	37.41828194161609
>50	0.02200392927308448	0.45607763277784136
>100	0.006286836935166995	0.6129165567134528
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	463	0.23052832311805738	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.21210597332244588	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.03335939017043163	0.0
2	0.0	0.0	0.0	0.11252570415697834	0.0
3	0.0	0.0	0.0	0.16231583873971211	0.0
4	0.0	0.0	0.0	0.27235203616755377	0.0
5	0.0	0.0	0.0	0.4735041798817982	0.0
6	0.0	0.0	0.0	0.7289275702912225	0.0
7	0.0	0.0	0.0	0.8509134000189202	0.0
8	0.0	0.0	0.0	1.2019338488271933	0.0
9	0.0	0.0	0.0	1.3159532570216537	0.0
10	0.0	0.0	0.0	1.5195949074650348	0.0
11	0.0	0.0	0.0	1.7486295265456102	0.0
12	0.0	0.0	0.0	1.960237598522229	0.0
13	0.0	0.0	0.0	2.0558346569210775	0.0
14	0.0	0.0	0.0	2.0891940470915094	0.0
15	0.0	0.0	0.0	2.141971589749207	0.0
16	0.0	0.0	0.0	2.2539993925603583	0.0
17	0.0	0.0	0.0	2.392913868046185	0.0
18	0.0	0.0	0.0	2.566183536394099	0.0
19	0.0	0.0	0.0	2.653316271913883	0.0
20	0.0	0.0	0.0	2.7334783885920846	0.0
21	0.0	0.0	0.0	2.8569579223572643	0.0
22	0.0	0.0	0.0	2.982429061505753	0.0
23	0.0	0.0	0.0	3.134288971983091	0.0
24	0.0	0.0	0.0	3.24681467614007	0.0
25	0.0	0.0	0.0	3.33096000358489	0.0
26	0.0	0.0	0.0	3.4260591606379114	0.0
27	0.0	0.0	0.0	3.520162514999278	0.0
28	0.0	0.0	0.0	3.618746981473091	0.0
29	0.0	0.0	0.0	3.7178293492927312	0.0
30	0.0	0.0	0.0	3.8437983897870476	0.0
31	0.0	0.0	0.0	3.9463660670274794	0.0
32	0.0	0.0	0.0	4.049431645613738	0.0
33	0.0	0.0	0.0	4.156978336312443	0.0
34	0.0	0.0	0.0	4.271495645852731	0.0
35	0.0	0.0	0.0	4.41190382537604	0.0
36	0.0	0.0	0.0	4.541856076636975	0.0
37	0.0	0.0	0.0	4.647411161952371	0.0
38	0.0	0.0	0.0	4.773380202446687	0.0
39	0.0	0.0	0.0	4.908809368511723	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTGATG	20	7.0193695E-4	45.000004	1
GGTACAT	20	7.0193695E-4	45.000004	8
CCCTCGT	20	7.0193695E-4	45.000004	14
GTACACT	20	7.0193695E-4	45.000004	18
GGATACA	20	7.0193695E-4	45.000004	8
GTTGTAG	20	7.0193695E-4	45.000004	1
GCTAGTT	20	7.0193695E-4	45.000004	9
TGAGCTT	20	7.0193695E-4	45.000004	30
CGATCTG	20	7.0193695E-4	45.000004	10
CGATAGT	20	7.0193695E-4	45.000004	12
TACGTAG	20	7.0193695E-4	45.000004	1
CTAGCGA	20	7.0193695E-4	45.000004	14
ACTATAG	25	3.879275E-5	45.0	1
CGACAGG	25	3.879275E-5	45.0	2
CAGGGCG	55	1.8189894E-12	45.0	5
AATGCGG	25	3.879275E-5	45.0	2
CTTGCGG	25	3.879275E-5	45.0	2
GTACTGG	25	3.879275E-5	45.0	2
GCGATTG	25	3.879275E-5	45.0	9
GCGATCT	25	3.879275E-5	45.0	9
>>END_MODULE