FastQCFastQC Report
Sat 18 Jun 2016
SRR3548992_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548992_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6000762
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239550.39919930168868556No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC72110.12016807198819084No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTTTT262600.042.6865961
TACGCTA403.4614823E-739.37524
CGCAATA356.2526997E-638.57142621
TCGATAG3250.038.0769231
TACGGGT9800.037.8826524
CTACGAA4650.037.74193611
CGAGGGA29350.037.6405454
ACGACGT301.1404962E-437.50000415
TACGGGA15600.037.54
GACCGAT31450.037.201919
CTATACG1650.036.818181
TAACGCG2200.036.818181
TAACGGG20000.036.4499973
ACGTAGG10500.036.02
CGATCGA504.884896E-836.010
TCGTCCC13350.035.89887638
CTACGGG12200.035.778693
TGGGCGA35650.035.72236
TAGTCCG1450.035.689661
CATACGA11250.035.618
Produced by FastQC (version 0.11.5)