##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548992_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6000762
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.18699658476707	31.0	31.0	33.0	30.0	34.0
2	31.555254316035196	31.0	31.0	34.0	30.0	34.0
3	31.66221773168141	31.0	31.0	34.0	30.0	34.0
4	35.48129587542382	37.0	35.0	37.0	33.0	37.0
5	35.40139552276861	37.0	35.0	37.0	33.0	37.0
6	35.468287694129515	37.0	35.0	37.0	33.0	37.0
7	35.89125664373958	37.0	35.0	37.0	35.0	37.0
8	35.94860935994462	37.0	35.0	37.0	35.0	37.0
9	37.829183860316405	39.0	38.0	39.0	35.0	39.0
10	37.10107966288281	39.0	37.0	39.0	33.0	39.0
11	36.76651731896716	39.0	35.0	39.0	32.0	39.0
12	36.19117072131839	38.0	35.0	39.0	32.0	39.0
13	35.9810964007571	38.0	35.0	39.0	31.0	39.0
14	36.98923753349991	39.0	35.0	41.0	31.0	41.0
15	37.18831425075682	39.0	35.0	41.0	32.0	41.0
16	37.246988299152676	39.0	35.0	41.0	32.0	41.0
17	37.180428252278624	39.0	35.0	41.0	32.0	41.0
18	37.07477966964862	39.0	35.0	41.0	32.0	41.0
19	37.026169176514586	38.0	35.0	41.0	32.0	41.0
20	36.867562486230916	38.0	35.0	41.0	32.0	41.0
21	36.77072545120103	38.0	35.0	41.0	31.0	41.0
22	36.66004667407239	38.0	35.0	41.0	31.0	41.0
23	36.57834571676064	38.0	35.0	40.0	31.0	41.0
24	36.484531964440514	38.0	35.0	40.0	31.0	41.0
25	36.37665483150307	38.0	35.0	40.0	31.0	41.0
26	36.21935964132555	38.0	35.0	40.0	30.0	41.0
27	36.09362894245764	38.0	35.0	40.0	30.0	41.0
28	36.02216801799505	38.0	35.0	40.0	30.0	41.0
29	36.130177634107135	38.0	35.0	40.0	30.0	41.0
30	36.0847667346247	38.0	35.0	40.0	30.0	41.0
31	35.94688374576429	38.0	35.0	40.0	30.0	41.0
32	35.7710579089789	38.0	34.0	40.0	29.0	41.0
33	35.52452421875755	38.0	34.0	40.0	28.0	41.0
34	35.326039093035185	38.0	34.0	40.0	27.0	41.0
35	35.139417960585675	38.0	34.0	40.0	26.0	41.0
36	34.979334457857185	38.0	34.0	40.0	25.0	41.0
37	34.87178511662352	38.0	34.0	40.0	25.0	41.0
38	34.81132679483039	37.0	34.0	40.0	25.0	41.0
39	34.74753856260255	37.0	34.0	40.0	24.0	41.0
40	34.62717084930214	37.0	33.0	40.0	24.0	41.0
41	34.56627891591101	37.0	33.0	40.0	23.0	41.0
42	34.51467763594024	37.0	33.0	40.0	23.0	41.0
43	34.414040250221554	37.0	33.0	40.0	23.0	41.0
44	34.3084258299196	37.0	33.0	40.0	23.0	41.0
45	34.26953176946528	37.0	33.0	40.0	23.0	41.0
46	34.26107884298694	36.0	33.0	40.0	23.0	41.0
47	34.22658189076654	36.0	33.0	40.0	23.0	41.0
48	34.127514472328684	36.0	33.0	40.0	23.0	41.0
49	34.080383624613006	36.0	33.0	40.0	23.0	41.0
50	33.954794907713385	36.0	33.0	40.0	23.0	41.0
51	33.72058348589729	35.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	5.0
11	14.0
12	10.0
13	23.0
14	51.0
15	150.0
16	448.0
17	1238.0
18	2896.0
19	5679.0
20	10245.0
21	16446.0
22	25003.0
23	37610.0
24	54888.0
25	80379.0
26	105831.0
27	117573.0
28	124266.0
29	137446.0
30	160750.0
31	195004.0
32	240180.0
33	307481.0
34	497871.0
35	697341.0
36	448886.0
37	563964.0
38	811541.0
39	1356862.0
40	679.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.963174010234034	23.881150427229077	29.385568032859826	14.770107529677064
2	32.42558195109221	25.152605619086376	29.50380301701684	12.918009412804574
3	28.38572834583341	24.658235070812673	33.119377172432436	13.836659410921479
4	25.527024734525384	27.644389162576356	32.341559288637015	14.487026814261256
5	22.68530229994124	32.3181289309591	31.14144503648037	13.85512373261929
6	20.7243846698136	40.57986302406261	27.90742242401882	10.788329882104973
7	87.9079856858179	3.422881960657663	7.232248171148931	1.436884182375505
8	89.13198023850971	2.429924732892256	6.80191948955816	1.6361755390398753
9	84.22042067324116	4.537073791628464	8.70739416094156	2.5351113741888116
10	44.2318992154663	24.771420696238245	17.737530666938632	13.259149421356822
11	37.27724912269475	24.825247193606412	22.00313893468863	15.89436474901021
12	31.541444236581956	23.452621517067332	28.006143219811086	16.99979102653963
13	22.295851760159792	32.911186945924534	28.600917683454202	16.19204361046147
14	19.102557308555145	34.881686692456725	29.38608463391816	16.62967136506997
15	18.4090953782203	25.31830124240888	40.423682858943586	15.84892052042724
16	19.67423470552573	21.89556926270364	39.33482114438133	19.0953748873893
17	20.323202286642932	22.9968627317664	29.499986835005288	27.17994814658538
18	22.96593332646754	24.70247945177629	32.26985172883044	20.06173549292573
19	27.6513049509379	25.943238542038493	26.500484438476313	19.904972068547295
20	28.73573389512865	24.719643938553137	26.95129385234742	19.593328313970794
21	24.255369568064854	27.080360794179136	28.386278275992282	20.27799136176372
22	23.676776382732726	25.448268069955116	27.239190622790904	23.63576492452125
23	21.528465884832627	29.371519816983245	26.96799173171674	22.13202256646739
24	21.553779336690905	26.128548341027358	33.47639849739083	18.841273824890905
25	19.936418074904488	27.227108823846034	31.98188830018588	20.8545848010636
26	20.204717334231887	31.151777057647013	28.476000214639406	20.167505393481694
27	20.963187675165255	29.77208561179397	29.26873287092539	19.995993842115382
28	19.49890697214787	28.20768429076174	33.52957507729852	18.763833659791874
29	19.922836466435427	25.288688336581256	33.430354344998186	21.35812085198513
30	21.59632393352711	28.207167689703404	29.93903107638663	20.25747730038285
31	25.4743814202263	26.793013953894523	26.991272108442228	20.741332517436952
32	26.735254622662925	27.20537825029555	26.485236374980374	19.574130752061155
33	25.365628565172223	27.96866464625659	26.13478088282788	20.530925905743306
34	21.636202202320305	27.490525369944685	28.32708579343757	22.546186634297445
35	23.145160564608293	26.547111850128367	28.725201899358783	21.582525685904557
36	26.7419371073207	26.661597310474903	26.309375375993916	20.28709020621048
37	22.6479570427889	30.430668638416254	27.620092248284468	19.30128207051038
38	22.659788873479737	30.036335385406055	26.051908074341224	21.251967666772988
39	22.833066867174537	29.781867702801744	26.48077027550834	20.904295154515378
40	23.63224870441454	26.810378415274595	26.368401213045946	23.188971667264923
41	20.14389172575083	26.07935458863391	27.399903545583044	26.376850140032214
42	22.3237148882092	28.04657141876315	25.27570665192187	24.35400704110578
43	22.78042355287545	27.66570312237013	26.685944218417596	22.867929106336828
44	21.785016636220533	29.874239304941607	26.644716121052625	21.696027937785235
45	20.804374511103756	31.511831330754326	24.70342933114161	22.980364827000304
46	22.51299085016203	30.267955969591863	26.29269416117486	20.926359019071246
47	22.997296010073388	27.053080925389146	27.316997407995853	22.63262565654162
48	23.519113072639776	25.245060543977583	28.81992320308654	22.4159031802961
49	21.800681313473188	26.008346939938626	28.714569916287296	23.476401830300887
50	20.895696246576684	29.53228273342619	27.516572062014795	22.05544895798234
51	20.42145647502767	30.121541230930337	25.954470448919654	23.502531845122338
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1219.0
1	3138.0
2	5057.0
3	25310.5
4	45564.0
5	35304.0
6	25044.0
7	25496.5
8	25949.0
9	26949.0
10	27949.0
11	28612.0
12	29275.0
13	28708.0
14	28141.0
15	28446.0
16	28751.0
17	27415.0
18	26079.0
19	25259.0
20	24439.0
21	25263.0
22	26087.0
23	29850.5
24	33614.0
25	35536.5
26	44164.0
27	50869.0
28	60735.0
29	70601.0
30	85782.0
31	100963.0
32	115503.0
33	130043.0
34	150618.0
35	171193.0
36	182560.5
37	193928.0
38	222425.0
39	250922.0
40	310672.0
41	370422.0
42	419103.5
43	467785.0
44	483336.5
45	498888.0
46	492041.0
47	485194.0
48	484405.5
49	483617.0
50	480207.0
51	476797.0
52	450031.5
53	423266.0
54	379722.5
55	336179.0
56	306438.0
57	276697.0
58	249119.5
59	221542.0
60	202355.5
61	183169.0
62	163106.5
63	143044.0
64	123503.5
65	103963.0
66	86057.0
67	68151.0
68	57006.5
69	45862.0
70	38419.0
71	30976.0
72	26486.0
73	21996.0
74	18375.5
75	11495.0
76	8235.0
77	5848.0
78	3461.0
79	2810.5
80	2160.0
81	1563.0
82	966.0
83	632.5
84	299.0
85	193.0
86	87.0
87	67.5
88	48.0
89	39.5
90	31.0
91	23.0
92	15.0
93	10.5
94	6.0
95	5.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	6000762.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.502783503343256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.84026056226186	14.587642725307026
2	8.890162558315208	3.2898550625206897
3	2.9219083046082175	1.6219031033035978
4	1.3835496588036538	1.0239807921187376
5	0.836006703961254	0.773422552536933
6	0.5715052383387129	0.6344662617604677
7	0.3915074308840353	0.5070784063518199
8	0.3113449088136161	0.46085979541171895
9	0.2501885722923425	0.41662664893221807
>10	2.9800058470316015	12.916028151992744
>50	1.1494356505294352	15.452862286352337
>100	1.4639501461652928	46.323434897448585
>500	0.008630086692491531	1.0358230060326454
>1k	0.0013626452668126258	0.4321630069205458
>5k	9.084301778750837E-5	0.12120599910170962
>10k+	9.084301778750837E-5	0.4026473039081201
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	23955	0.39919930168868556	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	7211	0.12016807198819084	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0340623407493915	0.0
2	0.0	0.0	0.0	0.13139997886935026	0.0
3	0.0	0.0	0.0	0.23090400852425075	0.0
4	1.666455026878253E-5	0.0	0.0	0.3611707979753238	0.0
5	1.666455026878253E-5	0.0	0.0	0.6237041229097238	0.0
6	1.666455026878253E-5	0.0	0.0	1.040167898676868	0.0
7	1.666455026878253E-5	0.0	0.0	1.2767711833930424	0.0
8	1.666455026878253E-5	0.0	0.0	1.788156237491172	0.0
9	1.666455026878253E-5	0.0	0.0	2.041657376179892	0.0
10	1.666455026878253E-5	0.0	0.0	2.3865969021934212	0.0
11	1.666455026878253E-5	0.0	0.0	2.742351721331391	0.0
12	1.666455026878253E-5	0.0	0.0	3.028698688599881	0.0
13	1.666455026878253E-5	0.0	0.0	3.1746468198538786	0.0
14	1.666455026878253E-5	0.0	0.0	3.2415383246327716	0.0
15	1.666455026878253E-5	0.0	0.0	3.323044639997387	1.666455026878253E-5
16	1.666455026878253E-5	0.0	0.0	3.490006769140319	1.666455026878253E-5
17	1.666455026878253E-5	0.0	0.0	3.689031493000389	1.666455026878253E-5
18	1.666455026878253E-5	0.0	0.0	3.9253181512614566	1.666455026878253E-5
19	1.666455026878253E-5	0.0	0.0	4.060217685687251	1.666455026878253E-5
20	1.666455026878253E-5	0.0	0.0	4.199233364029435	1.666455026878253E-5
21	1.666455026878253E-5	0.0	0.0	4.390592394765865	1.666455026878253E-5
22	1.666455026878253E-5	0.0	0.0	4.5983493429667766	1.666455026878253E-5
23	1.666455026878253E-5	0.0	0.0	4.838318866837245	1.666455026878253E-5
24	1.666455026878253E-5	0.0	0.0	5.02084568593122	1.666455026878253E-5
25	1.666455026878253E-5	0.0	0.0	5.1822085261838415	1.666455026878253E-5
26	1.666455026878253E-5	0.0	0.0	5.331122947385682	1.666455026878253E-5
27	1.666455026878253E-5	0.0	0.0	5.484303493456331	1.666455026878253E-5
28	1.666455026878253E-5	0.0	0.0	5.653182045880174	1.666455026878253E-5
29	1.666455026878253E-5	0.0	0.0	5.8342257200002265	1.666455026878253E-5
30	1.666455026878253E-5	0.0	0.0	6.059780407888198	1.666455026878253E-5
31	1.666455026878253E-5	0.0	0.0	6.260054973018427	1.666455026878253E-5
32	3.332910053756506E-5	0.0	0.0	6.451330681003513	1.666455026878253E-5
33	3.332910053756506E-5	0.0	0.0	6.647205804862782	1.666455026878253E-5
34	3.332910053756506E-5	0.0	0.0	6.851779823962357	1.666455026878253E-5
35	3.332910053756506E-5	0.0	0.0	7.0830671171427895	1.666455026878253E-5
36	3.332910053756506E-5	0.0	0.0	7.295673449471917	1.666455026878253E-5
37	3.332910053756506E-5	0.0	0.0	7.507729851642175	1.666455026878253E-5
38	3.332910053756506E-5	0.0	0.0	7.72516890354925	1.666455026878253E-5
39	3.332910053756506E-5	0.0	0.0	7.948057263394215	1.666455026878253E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTTTT	26260	0.0	42.686596	1
TACGCTA	40	3.4614823E-7	39.375	24
CGCAATA	35	6.2526997E-6	38.571426	21
TCGATAG	325	0.0	38.076923	1
TACGGGT	980	0.0	37.882652	4
CTACGAA	465	0.0	37.741936	11
CGAGGGA	2935	0.0	37.640545	4
ACGACGT	30	1.1404962E-4	37.500004	15
TACGGGA	1560	0.0	37.5	4
GACCGAT	3145	0.0	37.20191	9
CTATACG	165	0.0	36.81818	1
TAACGCG	220	0.0	36.81818	1
TAACGGG	2000	0.0	36.449997	3
ACGTAGG	1050	0.0	36.0	2
CGATCGA	50	4.884896E-8	36.0	10
TCGTCCC	1335	0.0	35.898876	38
CTACGGG	1220	0.0	35.77869	3
TGGGCGA	3565	0.0	35.7223	6
TAGTCCG	145	0.0	35.68966	1
CATACGA	1125	0.0	35.6	18
>>END_MODULE