FastQCFastQC Report
Sat 18 Jun 2016
SRR3548990_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR3548990_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2302145
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25580.11111376564030503No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATCCG302.1664546E-645.0000041
ATGCGAT302.1664546E-645.00000412
TTACGCG302.1664546E-645.0000041
ACACCGT207.0347625E-445.018
CGCATCG351.2126475E-745.021
TCTAGTC453.8562575E-1045.013
TCGATAG1400.045.01
TATTGCG1400.045.01
TCTACCG253.8920294E-545.01
GTCCGTT207.0347625E-445.010
TATTACG551.8189894E-1245.01
TATAGCG750.045.01
ACGTATG207.0347625E-445.01
ACGTAAG900.045.01
TCGACGA207.0347625E-445.029
TATCGGA207.0347625E-445.043
TAGTCGA253.8920294E-545.013
TACCGAA406.8193913E-945.013
TAATCGC207.0347625E-445.014
TTAACGA650.044.99999639
Produced by FastQC (version 0.11.5)