##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548990_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2302145
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24151693312107	31.0	31.0	33.0	30.0	34.0
2	31.56635442163721	31.0	31.0	34.0	30.0	34.0
3	31.692439876723665	31.0	31.0	34.0	30.0	34.0
4	30.336827176394188	35.0	33.0	37.0	0.0	37.0
5	32.90273158293678	35.0	33.0	37.0	19.0	37.0
6	34.52472976289504	35.0	33.0	37.0	30.0	37.0
7	35.46975668344088	36.0	35.0	37.0	33.0	37.0
8	35.63526580645441	37.0	35.0	37.0	35.0	37.0
9	37.67056853499671	39.0	37.0	39.0	35.0	39.0
10	37.148577522267274	39.0	37.0	39.0	33.0	39.0
11	36.66888097839189	39.0	35.0	39.0	32.0	39.0
12	35.4555025856321	37.0	35.0	39.0	31.0	39.0
13	35.01624702179924	37.0	34.0	39.0	30.0	39.0
14	35.764536551781056	37.0	34.0	40.0	30.0	41.0
15	36.059114000204154	37.0	35.0	40.0	31.0	41.0
16	36.21886414626359	37.0	35.0	40.0	31.0	41.0
17	36.10687076617676	37.0	34.0	40.0	31.0	41.0
18	36.09640053080931	36.0	34.0	40.0	30.0	41.0
19	36.137293697833975	37.0	35.0	40.0	31.0	41.0
20	36.046344170328105	36.0	34.0	40.0	31.0	41.0
21	35.88929498359139	36.0	34.0	40.0	30.0	41.0
22	35.770190409379076	36.0	34.0	40.0	30.0	41.0
23	35.72088161258305	36.0	34.0	40.0	30.0	41.0
24	35.636894287718626	36.0	34.0	40.0	30.0	41.0
25	35.51422521170473	35.0	34.0	40.0	30.0	41.0
26	35.389206153391726	35.0	34.0	40.0	29.0	41.0
27	35.42071416005508	35.0	34.0	40.0	30.0	41.0
28	35.4636202324354	36.0	34.0	40.0	30.0	41.0
29	35.49480289034791	36.0	34.0	40.0	30.0	41.0
30	35.38401360470344	36.0	34.0	40.0	29.0	41.0
31	35.26570003192675	35.0	34.0	40.0	29.0	41.0
32	35.15871806510884	35.0	34.0	40.0	29.0	41.0
33	35.03261610367722	35.0	34.0	40.0	29.0	41.0
34	34.855434388363896	35.0	34.0	40.0	27.0	41.0
35	34.778554348227416	35.0	33.0	40.0	27.0	41.0
36	34.66340434681569	35.0	33.0	40.0	27.0	41.0
37	34.6579685467249	35.0	33.0	40.0	27.0	41.0
38	34.65816010720437	35.0	33.0	40.0	27.0	41.0
39	34.61666793360106	35.0	33.0	40.0	27.0	41.0
40	34.49677800486069	35.0	33.0	40.0	27.0	41.0
41	34.49276348796449	35.0	33.0	39.0	27.0	41.0
42	34.44446765950885	35.0	33.0	39.0	27.0	41.0
43	34.36426202519824	35.0	33.0	39.0	27.0	41.0
44	34.277520312578055	35.0	33.0	39.0	27.0	41.0
45	34.15541723045247	35.0	33.0	39.0	26.0	41.0
46	34.10215603274337	35.0	33.0	39.0	26.0	41.0
47	34.03565196805588	35.0	33.0	39.0	26.0	41.0
48	33.95291260976177	35.0	33.0	39.0	26.0	41.0
49	33.91115416274822	35.0	33.0	38.0	26.0	40.0
50	33.80688053967061	35.0	33.0	38.0	26.0	40.0
51	33.7223185333678	35.0	33.0	38.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	2.0
11	1.0
12	2.0
13	5.0
14	13.0
15	47.0
16	153.0
17	497.0
18	974.0
19	2015.0
20	3637.0
21	5976.0
22	9196.0
23	13334.0
24	19285.0
25	26081.0
26	33835.0
27	41710.0
28	50291.0
29	61516.0
30	75204.0
31	94597.0
32	121022.0
33	179430.0
34	270094.0
35	354734.0
36	165924.0
37	205171.0
38	261050.0
39	306191.0
40	158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.47857975931143	25.497438258667458	29.75294779433963	12.271034187681488
2	28.50115870199314	26.010307778180785	31.811549663466028	13.676983856360048
3	29.14859837238749	25.845809017242615	30.621529052253443	14.384063558116452
4	22.59292963735994	38.707857237489385	26.0070065091469	12.692206616003771
5	23.990669571204247	31.880311622421697	29.264750917079507	14.864267889294549
6	22.182573208898656	41.34465900279956	25.80002562827276	10.672742160029017
7	90.5976817272587	3.6016845159622877	4.089316702466612	1.7113170543123915
8	92.4366188923808	2.235958204196521	3.536614765794509	1.7908081376281686
9	88.90956043168437	3.9460590014964305	4.67985291977699	2.464527647042215
10	62.14938676755808	20.027626409283517	8.57899915079198	9.243987672366423
11	53.35741232633045	18.09060680365485	15.653184312890803	12.898796557123902
12	47.57237272196148	19.666615265328637	18.36656683223689	14.394445180472996
13	24.227535624385084	37.46158473944951	19.972764530470496	18.338115105694907
14	16.613940477250562	38.14325335719514	27.535754698335683	17.707051467218616
15	17.738239772038686	21.126080242556398	45.7738761025044	15.361803882900512
16	22.290125079002408	17.075292824735193	42.354760451665726	18.27982164459667
17	20.523555206122985	18.87661289797124	26.275278055900042	34.324553840005734
18	25.981856051638797	23.08885843419941	30.16134952403085	20.767935990130944
19	33.2456904321839	26.3873908898006	20.34533011604395	20.021588561971555
20	37.44455714127477	23.928814214569456	20.936517899611015	17.69011074454476
21	27.515252080125276	29.905240547402528	22.834139465585356	19.745367906886838
22	25.729786785801938	24.535422399544775	24.153213633372356	25.581577181280934
23	25.935160469909587	28.962337298475987	22.845129216448136	22.25737301516629
24	26.626081328500163	21.28097926064605	34.319297872201794	17.773641538651997
25	21.314296015237964	22.77901696026966	31.80403493263891	24.102652091853464
26	19.17850526356941	33.88600631150514	25.291717072556246	21.643771352369203
27	19.54147110629435	31.7881801537262	26.604449328778163	22.065899411201293
28	17.018519684902557	28.8613010909391	34.82469609863844	19.295483125519898
29	20.153813074328504	25.22738576414605	33.291821323157315	21.326979838368132
30	22.69757117818382	28.774599341049328	28.78893379869643	19.738895682070417
31	34.22881703802324	23.830688336312438	21.857181020309323	20.083313605355006
32	36.90797061001805	24.922192129514	21.44751959585517	16.722317664612785
33	33.59636339153268	25.26535035803566	21.967469468691156	19.170816781740506
34	21.95713128408506	26.08601977720778	28.048537342348112	23.90831159635905
35	21.444826455327533	26.580949505786993	30.066351163805926	21.907872875079544
36	30.88584776371601	28.01135462796653	22.271099344307157	18.831698264010306
37	25.195372142067505	32.887676493009785	23.72126864293952	18.195682721983193
38	22.713730021349654	35.41379887018411	19.831461528270374	22.041009580195862
39	24.89178570420195	31.278525027745864	22.519172337103004	21.31051693094918
40	27.04356154803455	24.16611464525475	21.7447206844052	27.0456031223055
41	17.540511132009495	24.99894663455169	24.41357950954436	33.04696272389446
42	21.693246950126944	25.615458626628644	21.410119692721352	31.281174730523055
43	22.073805081782425	25.013498280951023	23.367511603309087	29.54518503395746
44	21.176468033073505	27.715891049434333	25.639436264874714	25.468204652617448
45	20.961103666363325	34.09863409993723	20.499447254625576	24.440814979073863
46	22.407233254204233	33.02359321415462	22.967015544198997	21.602157987442148
47	22.063901274680788	25.2160485112797	25.749246898001648	26.970803316037866
48	23.7379487391107	20.97022559395694	31.91766808780507	23.374157579127292
49	23.753412578269398	22.22101561804317	30.180244945474765	23.84532685821267
50	19.635253209506786	31.027541705670146	24.67529195598018	24.661913128842883
51	18.376557514839423	32.11027107328165	24.506145355744316	25.007026056134606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	389.0
1	865.5
2	1342.0
3	3898.0
4	6454.0
5	4626.0
6	2798.0
7	2915.0
8	3032.0
9	3188.0
10	3344.0
11	3459.0
12	3574.0
13	3496.5
14	3419.0
15	3305.5
16	3192.0
17	3314.5
18	3437.0
19	3788.5
20	4140.0
21	4032.0
22	3924.0
23	4498.5
24	5073.0
25	6254.5
26	9772.0
27	12108.0
28	16118.5
29	20129.0
30	22221.5
31	24314.0
32	28950.5
33	33587.0
34	36691.5
35	39796.0
36	45342.5
37	50889.0
38	65286.0
39	79683.0
40	114957.5
41	150232.0
42	181103.5
43	211975.0
44	212655.5
45	213336.0
46	196083.5
47	178831.0
48	167513.5
49	156196.0
50	154632.0
51	153068.0
52	144030.5
53	134993.0
54	129445.5
55	123898.0
56	125557.5
57	127217.0
58	130258.5
59	133300.0
60	131761.5
61	130223.0
62	117603.5
63	104984.0
64	84909.5
65	64835.0
66	51860.5
67	38886.0
68	32569.0
69	26252.0
70	21872.0
71	17492.0
72	14588.0
73	11684.0
74	8625.0
75	4660.5
76	3755.0
77	2877.0
78	1999.0
79	1381.5
80	764.0
81	594.5
82	425.0
83	267.5
84	110.0
85	70.5
86	31.0
87	26.0
88	21.0
89	11.5
90	2.0
91	5.5
92	9.0
93	5.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2302145.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.32575802045377
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.5414844881864	12.822492703213545
2	8.370850625887252	2.733209634871985
3	2.7957874696828906	1.369300491199788
4	1.2871758077100524	0.8405648306582578
5	0.7271487607573721	0.5935627356498844
6	0.49102591016597197	0.4809822114685638
7	0.36247111203024257	0.4142330965087371
8	0.2602384282166041	0.3398871685350005
9	0.22138939501672775	0.32529147222039756
>10	3.42539556731817	14.650540199077772
>50	1.7691052586235472	20.77508260730834
>100	1.739515313126484	43.623267440037736
>500	0.006961542023404611	0.69777869102549
>1k	0.0014503212548759605	0.3338067182245456
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2558	0.11111376564030503	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.059596593611610044	0.0
2	0.0	0.0	0.0	0.18673888916640785	0.0
3	0.0	0.0	0.0	0.2680977957513536	0.0
4	0.0	0.0	0.0	0.4189136652991015	0.0
5	0.0	0.0	0.0	0.6941786898740088	0.0
6	0.0	0.0	0.0	0.9593227185950494	0.0
7	0.0	0.0	0.0	1.105056371340641	0.0
8	0.0	0.0	0.0	1.4440445758195075	0.0
9	0.0	0.0	0.0	1.5489901808965116	0.0
10	0.0	0.0	0.0	1.7232624356849808	0.0
11	0.0	0.0	0.0	2.0213757169943682	0.0
12	0.0	0.0	0.0	2.229659730381883	0.0
13	0.0	0.0	0.0	2.3254834078652733	0.0
14	0.0	0.0	0.0	2.3673139615445593	0.0
15	0.0	0.0	0.0	2.431167454699856	0.0
16	0.0	0.0	0.0	2.5632182160550268	0.0
17	0.0	0.0	0.0	2.713599708098317	0.0
18	0.0	0.0	0.0	2.8897832239064005	0.0
19	0.0	0.0	0.0	3.018185214224126	0.0
20	0.0	0.0	0.0	3.14124436123702	0.0
21	0.0	0.0	0.0	3.2980980780967317	0.0
22	0.0	0.0	0.0	3.454560855202431	0.0
23	4.343775044577991E-5	0.0	0.0	3.6293978007466947	0.0
24	4.343775044577991E-5	0.0	0.0	3.76696515640848	0.0
25	4.343775044577991E-5	0.0	0.0	3.8825964480951463	0.0
26	4.343775044577991E-5	0.0	0.0	3.9930152097283185	0.0
27	4.343775044577991E-5	0.0	0.0	4.096310180288383	0.0
28	4.343775044577991E-5	0.0	0.0	4.214460861500904	0.0
29	4.343775044577991E-5	0.0	0.0	4.339474707283859	0.0
30	4.343775044577991E-5	0.0	0.0	4.489204633070463	0.0
31	8.687550089155982E-5	0.0	0.0	4.630985450525488	0.0
32	1.3031325133733973E-4	0.0	0.0	4.764122155641804	0.0
33	1.3031325133733973E-4	0.0	0.0	4.9056423465941545	0.0
34	1.3031325133733973E-4	0.0	0.0	5.058065412908396	0.0
35	1.3031325133733973E-4	0.0	0.0	5.240677715782455	0.0
36	1.3031325133733973E-4	0.0	0.0	5.4031783402001174	0.0
37	1.3031325133733973E-4	0.0	0.0	5.569023671402105	0.0
38	1.3031325133733973E-4	0.0	0.0	5.761496343627356	0.0
39	1.3031325133733973E-4	0.0	0.0	5.97143099153181	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTATCCG	30	2.1664546E-6	45.000004	1
ATGCGAT	30	2.1664546E-6	45.000004	12
TTACGCG	30	2.1664546E-6	45.000004	1
ACACCGT	20	7.0347625E-4	45.0	18
CGCATCG	35	1.2126475E-7	45.0	21
TCTAGTC	45	3.8562575E-10	45.0	13
TCGATAG	140	0.0	45.0	1
TATTGCG	140	0.0	45.0	1
TCTACCG	25	3.8920294E-5	45.0	1
GTCCGTT	20	7.0347625E-4	45.0	10
TATTACG	55	1.8189894E-12	45.0	1
TATAGCG	75	0.0	45.0	1
ACGTATG	20	7.0347625E-4	45.0	1
ACGTAAG	90	0.0	45.0	1
TCGACGA	20	7.0347625E-4	45.0	29
TATCGGA	20	7.0347625E-4	45.0	43
TAGTCGA	25	3.8920294E-5	45.0	13
TACCGAA	40	6.8193913E-9	45.0	13
TAATCGC	20	7.0347625E-4	45.0	14
TTAACGA	65	0.0	44.999996	39
>>END_MODULE