##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548986_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4361256
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.218437074090584	31.0	31.0	33.0	30.0	34.0
2	31.541663685873978	31.0	31.0	34.0	30.0	34.0
3	31.65930617235035	31.0	31.0	34.0	30.0	34.0
4	30.329971916347034	35.0	33.0	37.0	0.0	37.0
5	32.86593242863982	35.0	33.0	37.0	19.0	37.0
6	34.4801128390537	35.0	33.0	37.0	30.0	37.0
7	35.44912153746535	36.0	35.0	37.0	33.0	37.0
8	35.61739829076761	37.0	35.0	37.0	35.0	37.0
9	37.634924434612415	39.0	37.0	39.0	35.0	39.0
10	37.082289597308666	39.0	37.0	39.0	33.0	39.0
11	36.64829306053119	39.0	35.0	39.0	32.0	39.0
12	35.67494570371471	37.0	35.0	39.0	31.0	39.0
13	35.264866588890904	37.0	35.0	39.0	30.0	39.0
14	36.10833782745154	38.0	35.0	40.0	30.0	41.0
15	36.41090617014915	38.0	35.0	40.0	31.0	41.0
16	36.56826565558179	38.0	35.0	40.0	32.0	41.0
17	36.510120479054656	38.0	35.0	40.0	31.0	41.0
18	36.48823389408923	38.0	35.0	40.0	31.0	41.0
19	36.465918304268314	38.0	35.0	40.0	31.0	41.0
20	36.36075616748936	38.0	35.0	40.0	31.0	41.0
21	36.194565739777715	37.0	35.0	40.0	31.0	41.0
22	36.077115629075664	37.0	34.0	40.0	30.0	41.0
23	36.02568732493575	37.0	34.0	40.0	30.0	41.0
24	35.943588956942676	37.0	34.0	40.0	30.0	41.0
25	35.808034887197635	37.0	34.0	40.0	30.0	41.0
26	35.65598855008741	37.0	34.0	40.0	30.0	41.0
27	35.64106991197031	37.0	34.0	40.0	30.0	41.0
28	35.65007213518307	36.0	34.0	40.0	30.0	41.0
29	35.687920864998524	36.0	34.0	40.0	30.0	41.0
30	35.649210686095934	36.0	34.0	40.0	30.0	41.0
31	35.537591923060695	36.0	34.0	40.0	30.0	41.0
32	35.43879744734086	36.0	34.0	40.0	29.0	41.0
33	35.284408207177016	36.0	34.0	40.0	29.0	41.0
34	35.198210790652965	36.0	34.0	40.0	28.0	41.0
35	35.081382060580715	36.0	34.0	40.0	28.0	41.0
36	34.9088216788925	36.0	33.0	40.0	27.0	41.0
37	34.84245914479682	36.0	33.0	40.0	27.0	41.0
38	34.80964497383322	35.0	33.0	40.0	27.0	41.0
39	34.75162269768158	35.0	33.0	40.0	27.0	41.0
40	34.62007274968495	35.0	33.0	40.0	26.0	41.0
41	34.58733928941571	35.0	33.0	40.0	26.0	41.0
42	34.51486154447251	35.0	33.0	40.0	26.0	41.0
43	34.446644728032474	35.0	33.0	40.0	26.0	41.0
44	34.3695174050778	35.0	33.0	39.0	26.0	41.0
45	34.27836361818706	35.0	33.0	39.0	26.0	41.0
46	34.20719031398294	35.0	33.0	39.0	26.0	41.0
47	34.117807576533	35.0	33.0	39.0	26.0	41.0
48	34.005536937065834	35.0	33.0	39.0	26.0	41.0
49	33.972766331533855	35.0	33.0	39.0	26.0	41.0
50	33.81375479907623	35.0	33.0	39.0	25.0	40.0
51	33.722627610027935	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	4.0
12	3.0
13	7.0
14	32.0
15	115.0
16	314.0
17	877.0
18	1997.0
19	3816.0
20	6831.0
21	11345.0
22	17078.0
23	25145.0
24	34866.0
25	47572.0
26	61502.0
27	76618.0
28	92456.0
29	113024.0
30	138679.0
31	172990.0
32	219926.0
33	316100.0
34	469546.0
35	618065.0
36	343510.0
37	427428.0
38	536078.0
39	624943.0
40	388.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.651648974515595	25.352306766674555	29.478320006897096	12.517724251912751
2	28.13116221565531	26.7221644407024	31.499480883488612	13.647192460153681
3	29.496525771475007	26.077625344625492	30.16835058524425	14.25749829865525
4	23.260134236559377	38.502165431242744	25.492266448014057	12.745433884183822
5	23.806100811325912	32.50274691510886	28.8059448929391	14.885207380626131
6	22.601883494112705	41.63364865534149	24.92655785397601	10.837909996569795
7	89.86094831397193	4.239604370850966	4.001301459946401	1.898145855230695
8	91.78195455621041	2.9871211412492182	3.4431594935037064	1.7877648090366627
9	88.16120860596122	4.5583428260115895	4.721690265373094	2.558758302654098
10	57.77895175151379	22.935411266846064	9.09483414869478	10.190802832945371
11	48.644335485007076	20.503405441001398	16.418297848142828	14.433961225848702
12	44.371896536227176	21.063542245628323	19.11967561638207	15.44488560176243
13	23.683980027771817	38.22871209578158	20.031683533367453	18.05562434307915
14	16.23016397111291	39.30888257878006	25.218239883189614	19.24271356691742
15	16.6869360569524	22.95634560319321	43.472981177899214	16.88373716195518
16	18.750676410648676	18.364503253191284	42.0043446199902	20.880475716169837
17	18.993198289667014	20.592599929928443	25.5702027122462	34.84399906815835
18	24.49149969641773	24.977208400515817	28.38017305106602	22.151118852000433
19	32.96078010554758	25.413527662673324	20.11049110623178	21.51520112554732
20	35.164709432328664	24.13630385375222	21.158354382315554	19.540632331603554
21	26.429748677903795	28.367584934248296	23.546404980583574	21.656261407264328
22	25.65735650463995	25.08311825767623	21.96454415883865	27.294981078845176
23	25.092312856663312	29.35085672567719	21.866774158636872	23.69005625902263
24	24.347504480360705	22.977646806332856	33.11830353457811	19.556545178728328
25	21.678594423257888	25.062413213074397	30.183254548689643	23.07573781497807
26	20.473161859794516	34.79759041890685	22.97159350425657	21.757654217042063
27	21.629938714902313	32.10093606062107	26.328355868126064	19.940769356350557
28	18.599985875628487	28.237255506211973	34.6523340982506	18.510424519908945
29	19.562827772549927	24.788340789900893	32.83421106213439	22.814620375414787
30	22.831106451902848	29.532983158979892	26.66988592277087	20.966024466346393
31	30.50082361594917	26.874689309685103	20.772914958443163	21.85157211592257
32	33.947972785821335	26.090419824014	21.408419959754713	18.553187430409956
33	30.351806910669772	26.96195774795151	21.44774349407602	21.238491847302704
34	21.93673107013209	26.701619900322292	25.70502625849067	25.656622771054945
35	24.084483919311317	24.515712904722857	27.115032917122956	24.284770258842865
36	30.80174610249891	26.20105309112788	22.345604110375543	20.651596695997668
37	23.635347248590772	32.2300273132327	25.138354639122305	18.99627079905422
38	23.37987038596221	32.78186375667927	21.23690973426004	22.601356123098483
39	23.922122434454664	30.281804140825486	23.883234554449455	21.9128388702704
40	26.64149960470103	24.8420638458279	24.0222770688077	24.49415948066337
41	19.05153010967483	23.748411008204975	26.156272413268105	31.04378646885209
42	23.1358351814248	25.958691716331263	21.99412279398412	28.91135030825982
43	23.215720425492105	26.526532723600727	23.589305466131773	26.66844138477539
44	22.224629785548018	28.956520782086624	25.524504867405167	23.294344564960188
45	19.844741973413164	34.04707267814593	21.078560854946375	25.029624493494534
46	22.682938126081112	32.017405077803275	22.87157644495072	22.428080351164894
47	23.039372144171313	26.197865935868016	26.043896528889842	24.71886539107083
48	24.049929653292537	21.60753691138516	30.025501827913793	24.317031607408506
49	21.982360127449525	24.164277446680497	29.1268616196802	24.726500806189776
50	19.77471627439435	31.145706649644044	25.172427392475928	23.907149683485677
51	19.395032073329336	32.78037336033473	22.702519641130902	25.122074925205034
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	605.0
1	1578.5
2	2552.0
3	6658.0
4	10764.0
5	8148.0
6	5532.0
7	5757.0
8	5982.0
9	6293.5
10	6605.0
11	6631.5
12	6658.0
13	6680.0
14	6702.0
15	6495.5
16	6289.0
17	6217.0
18	6145.0
19	5971.5
20	5798.0
21	7056.5
22	8315.0
23	7723.0
24	7131.0
25	11269.0
26	22770.5
27	30134.0
28	36460.0
29	42786.0
30	47490.5
31	52195.0
32	59938.0
33	67681.0
34	79639.0
35	91597.0
36	102417.0
37	113237.0
38	136121.0
39	159005.0
40	220307.5
41	281610.0
42	337474.5
43	393339.0
44	393529.5
45	393720.0
46	368741.5
47	343763.0
48	332443.0
49	321123.0
50	294980.5
51	268838.0
52	255695.5
53	242553.0
54	230005.0
55	217457.0
56	223175.0
57	228893.0
58	238439.0
59	247985.0
60	233696.0
61	219407.0
62	194956.5
63	170506.0
64	145425.5
65	120345.0
66	103367.5
67	86390.0
68	74941.0
69	63492.0
70	53928.5
71	44365.0
72	36293.5
73	28222.0
74	22438.0
75	14298.5
76	11943.0
77	8864.0
78	5785.0
79	3982.0
80	2179.0
81	1572.0
82	965.0
83	687.5
84	410.0
85	238.0
86	66.0
87	75.0
88	84.0
89	55.5
90	27.0
91	19.0
92	11.0
93	6.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4361256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	13.94088065605974
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.96933986464526	10.869612618847809
2	9.263974386494462	2.582959226458271
3	3.2958302658651286	1.378403291971662
4	1.717605335262147	0.9577972397240427
5	0.9631191397195495	0.6713364492198584
6	0.6582647018768958	0.5506073789377527
7	0.4489092466617964	0.43807331631796514
8	0.31378566239255795	0.34995587767978426
9	0.24221664037146745	0.30390419486973325
>10	2.0768988471242666	6.665038748631937
>50	0.8923911737421452	9.18400289933313
>100	2.1185851137631073	61.765142338869374
>500	0.034217180624113	2.993461380797093
>1k	0.004322170184098484	0.9001824829616191
>5k	5.402712730123105E-4	0.3895225553799894
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5397	0.12374875494582296	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5060	0.11602162312875006	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	5058	0.11597576477968732	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05314982656372384	0.0
2	0.0	0.0	0.0	0.1529834524733242	0.0
3	0.0	0.0	0.0	0.21645140757616613	0.0
4	0.0	0.0	0.0	0.37645118745609063	0.0
5	0.0	0.0	0.0	0.6430716289069021	0.0
6	0.0	0.0	0.0	0.9208815075290238	0.0
7	0.0	0.0	0.0	1.0647391485388613	0.0
8	0.0	0.0	0.0	1.4287168650498847	0.0
9	0.0	0.0	0.0	1.5322420880590362	0.0
10	2.2929174531373532E-5	0.0	0.0	1.7118233829887537	0.0
11	2.2929174531373532E-5	0.0	0.0	1.9847264182611615	0.0
12	2.2929174531373532E-5	0.0	0.0	2.2137659426550518	0.0
13	2.2929174531373532E-5	0.0	0.0	2.30488648224273	0.0
14	2.2929174531373532E-5	0.0	0.0	2.337789847695251	0.0
15	2.2929174531373532E-5	0.0	0.0	2.395846517608689	0.0
16	2.2929174531373532E-5	0.0	0.0	2.528262500527371	0.0
17	2.2929174531373532E-5	0.0	0.0	2.675995172033011	0.0
18	2.2929174531373532E-5	0.0	0.0	2.845304196772673	0.0
19	2.2929174531373532E-5	0.0	0.0	2.9530713170701284	0.0
20	2.2929174531373532E-5	0.0	0.0	3.0663872976041766	0.0
21	2.2929174531373532E-5	0.0	0.0	3.224346380950809	0.0
22	2.2929174531373532E-5	0.0	0.0	3.3865702907602766	0.0
23	2.2929174531373532E-5	0.0	0.0	3.561703325830907	0.0
24	2.2929174531373532E-5	0.0	0.0	3.6933167876409914	0.0
25	2.2929174531373532E-5	0.0	0.0	3.8115854698738163	0.0
26	2.2929174531373532E-5	0.0	0.0	3.916119576562348	0.0
27	2.2929174531373532E-5	0.0	0.0	4.015861485773823	0.0
28	2.2929174531373532E-5	0.0	0.0	4.127434849043486	0.0
29	2.2929174531373532E-5	0.0	0.0	4.245244947785684	0.0
30	2.2929174531373532E-5	0.0	0.0	4.390363693394747	0.0
31	2.2929174531373532E-5	0.0	0.0	4.534565272022555	0.0
32	2.2929174531373532E-5	0.0	0.0	4.6709709313096965	0.0
33	4.5858349062747064E-5	0.0	0.0	4.801552580265868	0.0
34	4.5858349062747064E-5	0.0	0.0	4.9474509178089985	0.0
35	4.5858349062747064E-5	0.0	0.0	5.125450099696051	0.0
36	4.5858349062747064E-5	0.0	0.0	5.282492016061428	0.0
37	6.878752359412059E-5	0.0	0.0	5.448017727003414	0.0
38	9.171669812549413E-5	0.0	0.0	5.599212703863291	0.0
39	1.1464587265686765E-4	0.0	0.0	5.761046817705725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	100	0.0	45.0	1
TCTAGCG	175	0.0	45.0	1
ATCGTAC	20	7.0354587E-4	45.0	27
TCGAATA	25	3.8926053E-5	45.0	41
ATTGGGC	1090	0.0	42.522938	4
TATTACG	75	0.0	42.0	1
CGTTTTT	4165	0.0	40.94838	1
CGTATTT	760	0.0	40.855267	1
ACTATCG	50	1.0822987E-9	40.5	1
ACTCGAT	50	1.0822987E-9	40.5	13
AATCGAC	50	1.0822987E-9	40.5	35
CGAATAT	685	0.0	40.07299	14
CGTATCA	45	1.9304935E-8	40.000004	22
CGCATTA	90	0.0	40.000004	12
ATCGATA	45	1.9304935E-8	40.000004	36
CAACCCG	85	0.0	39.705883	23
CGTATCT	40	3.4611912E-7	39.375	33
GCGAAGT	40	3.4611912E-7	39.375	37
TATATCG	40	3.4611912E-7	39.375	12
ATCCGTT	40	3.4611912E-7	39.375	17
>>END_MODULE