##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548984_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2166219
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.287508326720427	31.0	31.0	33.0	30.0	34.0
2	31.603663803151942	31.0	31.0	34.0	30.0	34.0
3	31.71995629250782	31.0	31.0	34.0	30.0	34.0
4	30.329895084476686	35.0	33.0	37.0	0.0	37.0
5	32.900390034433265	35.0	33.0	37.0	19.0	37.0
6	34.534030492761815	35.0	33.0	37.0	30.0	37.0
7	35.487257751870885	36.0	35.0	37.0	33.0	37.0
8	35.65741367793376	37.0	35.0	37.0	35.0	37.0
9	37.68099578112832	39.0	37.0	39.0	35.0	39.0
10	37.15122801526531	39.0	37.0	39.0	33.0	39.0
11	36.74701680670329	39.0	35.0	39.0	32.0	39.0
12	35.79360904876192	37.0	35.0	39.0	31.0	39.0
13	35.38594343415878	37.0	35.0	39.0	30.0	39.0
14	36.25886533171392	38.0	35.0	40.0	31.0	41.0
15	36.5329844304754	38.0	35.0	40.0	31.0	41.0
16	36.69330617079806	38.0	35.0	40.0	32.0	41.0
17	36.64420264063791	38.0	35.0	40.0	32.0	41.0
18	36.61284062230089	38.0	35.0	40.0	31.0	41.0
19	36.60088569068963	38.0	35.0	40.0	31.0	41.0
20	36.52030981170417	38.0	35.0	40.0	31.0	41.0
21	36.35450801604085	38.0	35.0	40.0	31.0	41.0
22	36.23971999137668	38.0	35.0	40.0	31.0	41.0
23	36.178458410714704	37.0	34.0	40.0	31.0	41.0
24	36.08984040856441	37.0	34.0	40.0	30.0	41.0
25	35.95440904174509	37.0	34.0	40.0	30.0	41.0
26	35.80406551692142	37.0	34.0	40.0	30.0	41.0
27	35.823251942670616	37.0	34.0	40.0	30.0	41.0
28	35.84081618709835	37.0	34.0	40.0	30.0	41.0
29	35.8733853779327	37.0	34.0	40.0	30.0	41.0
30	35.816731826283494	37.0	34.0	40.0	30.0	41.0
31	35.7367759215481	37.0	34.0	40.0	30.0	41.0
32	35.63701084701039	37.0	34.0	40.0	30.0	41.0
33	35.49251391479809	36.0	34.0	40.0	29.0	41.0
34	35.41883807685188	36.0	34.0	40.0	29.0	41.0
35	35.31046029972039	36.0	34.0	40.0	29.0	41.0
36	35.15643939970982	36.0	34.0	40.0	28.0	41.0
37	35.135748509268915	36.0	34.0	40.0	28.0	41.0
38	35.10225512748249	36.0	34.0	40.0	28.0	41.0
39	35.04164398890417	36.0	34.0	40.0	28.0	41.0
40	34.88538693456202	36.0	34.0	40.0	27.0	41.0
41	34.853211055761214	36.0	34.0	40.0	27.0	41.0
42	34.81828568579631	36.0	33.0	40.0	27.0	41.0
43	34.76358761510263	35.0	33.0	40.0	27.0	41.0
44	34.68349368184842	35.0	33.0	40.0	27.0	41.0
45	34.58919019729769	35.0	33.0	40.0	27.0	41.0
46	34.529285358497916	35.0	33.0	40.0	27.0	41.0
47	34.45586203426339	35.0	33.0	39.0	27.0	41.0
48	34.34370024452745	35.0	33.0	39.0	26.0	41.0
49	34.31048291977866	35.0	33.0	39.0	26.0	41.0
50	34.175230666890094	35.0	33.0	39.0	26.0	41.0
51	34.07439229366929	35.0	33.0	39.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	3.0
12	4.0
13	2.0
14	4.0
15	45.0
16	112.0
17	380.0
18	793.0
19	1559.0
20	2953.0
21	4895.0
22	7171.0
23	10754.0
24	15466.0
25	21213.0
26	28085.0
27	35095.0
28	42659.0
29	52810.0
30	65277.0
31	83185.0
32	105944.0
33	153415.0
34	229338.0
35	306799.0
36	168327.0
37	212588.0
38	276254.0
39	340907.0
40	182.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	33.37391094806203	24.25174924603653	30.6409924388993	11.733347367002137
2	28.758449630439024	26.83703725246616	30.838941030431364	13.565572086663444
3	29.783830720716605	26.34142715948849	30.105451018571994	13.769291101222914
4	23.06913566910825	38.52274400695405	25.37965921266502	13.028461111272682
5	24.061279122747976	32.89099578574465	28.434982797214868	14.612742294292497
6	23.05884123442736	40.645659557043864	25.374812057322	10.92068715120678
7	89.18318046328649	4.798268319131168	4.0585924137864176	1.9599588037959228
8	91.67503378005641	2.9963267795176756	3.4769337726240974	1.8517056678018242
9	87.789323240171	5.042380294882466	4.715220390920771	2.4530760740257564
10	56.85934801605932	23.540648475523483	9.328465866101258	10.271537642315943
11	49.261916731410814	20.67140949276135	16.30061411150027	13.766059664327567
12	45.468902267037635	20.788849142215078	19.511785281174248	14.230463309573038
13	23.13764213128959	38.03156559886143	20.41123265930176	18.419559610547225
14	15.423555974719083	41.04044881888673	24.893235633146972	18.64275957324721
15	15.983979459140556	24.042767605676065	44.07029021534757	15.902962719835806
16	18.018122821376785	18.904459798386036	43.408999736407075	19.668417643830104
17	18.06216268992193	19.600742122564707	26.718305028254296	35.61879015925906
18	23.143181737395896	24.998257332245725	29.3836865063043	22.47487442405408
19	32.48318844955197	25.207792933216815	20.990583131253118	21.318435485978103
20	36.21485177629778	22.860661825974198	21.073584896079296	19.850901501648725
21	25.450427680673098	28.254022331075486	23.466048446625205	22.829501541626215
22	25.26115780537425	26.209030573547736	21.842990020861233	26.68682160021678
23	24.757145976468674	30.305338472241267	21.414593815306763	23.522921735983296
24	23.825753536461455	23.139211686353043	33.14923375706704	19.885801020118464
25	20.975441541229213	25.417559351109002	30.681477726859562	22.92552138080222
26	19.95033743125695	35.686050210066476	23.02574208794217	21.3378702707344
27	21.68995840217448	33.126152064957424	25.890318568898156	19.29357096396994
28	18.82921348210869	29.349479438597854	33.67037220151794	18.150934877775516
29	19.2766751653457	25.609091232234597	33.18925741118511	21.924976191234588
30	21.37083092706693	29.449423165432485	27.05492842598094	22.124817481519642
31	30.317340952138267	27.31136602531877	21.270979527000733	21.100313495542235
32	32.76321553822582	27.43683810362664	21.522477644227106	18.27746871392043
33	29.527531611531426	28.557454255548492	21.68155666624658	20.2334574666735
34	21.850560815873187	27.775354200106268	25.62700262531166	24.747082358708884
35	22.94154007512629	24.555365824046415	27.83965979432366	24.663434306503635
36	29.323674106819304	26.305696700102803	23.671521669785005	20.699107523292888
37	23.64696274938037	31.272369044865734	25.248924508556154	19.831743697197744
38	22.00248451333868	32.54763253392201	21.278134851554714	24.171748101184598
39	23.99854308359404	30.52719969679889	23.60116867223489	21.87308854737217
40	26.59698765452616	25.09649301386425	24.13375563597217	24.17276369563742
41	18.68029040461745	25.258249512168433	26.444140689376283	29.617319393837832
42	23.443474551742	26.605666370759373	21.557054019007314	28.393805058491317
43	23.664458671999462	27.021552299190432	23.50044016786853	25.813548860941577
44	22.14716055948175	29.63624638136772	25.772278795449584	22.444314263700946
45	19.401962590116696	34.530765356595985	21.072984772084446	24.99428728120287
46	22.17495091678173	32.77664908303362	23.139673320195232	21.908726679989417
47	24.46663979957705	25.890918692893006	24.85547398485564	24.7869675226743
48	24.0826989330257	21.551699066437884	29.662836490677996	24.70276550985842
49	21.61960540462437	24.255626970310942	29.670130305384635	24.45463731968005
50	19.74846495206625	32.148088443504555	24.84762620953837	23.255820394890822
51	18.628910557981442	33.50880035675063	22.639123745106104	25.223165340161824
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	408.0
1	811.0
2	1214.0
3	3165.5
4	5117.0
5	3883.5
6	2650.0
7	2795.5
8	2941.0
9	3195.0
10	3449.0
11	3462.0
12	3475.0
13	3447.5
14	3420.0
15	3310.5
16	3201.0
17	3317.0
18	3433.0
19	3535.5
20	3638.0
21	3732.5
22	3827.0
23	4075.0
24	4323.0
25	6359.5
26	10788.5
27	13181.0
28	18209.0
29	23237.0
30	25964.5
31	28692.0
32	32133.0
33	35574.0
34	40558.5
35	45543.0
36	46139.5
37	46736.0
38	63411.5
39	80087.0
40	111927.5
41	143768.0
42	175166.0
43	206564.0
44	203914.5
45	201265.0
46	186061.0
47	170857.0
48	165879.5
49	160902.0
50	153677.5
51	146453.0
52	136748.5
53	127044.0
54	120731.0
55	114418.0
56	113362.5
57	112307.0
58	117576.5
59	122846.0
60	117209.5
61	111573.0
62	98402.5
63	85232.0
64	66373.0
65	47514.0
66	39470.5
67	31427.0
68	27322.5
69	23218.0
70	19497.0
71	15776.0
72	13146.0
73	10516.0
74	8407.5
75	4722.0
76	3145.0
77	2108.0
78	1071.0
79	973.5
80	876.0
81	594.5
82	313.0
83	235.5
84	158.0
85	125.5
86	93.0
87	60.0
88	27.0
89	20.0
90	13.0
91	7.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2166219.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.603486793843544
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.42946867013511	13.022026473127543
2	8.153641235114666	2.7075774913792903
3	2.6409974175052	1.3154929723236748
4	1.2620597850182744	0.8381837189436777
5	0.7348285662670144	0.6100358197876681
6	0.5032757968783793	0.5013679828278754
7	0.35292227826183564	0.41018182704614964
8	0.29349429430710694	0.3898422911677185
9	0.23804417116489465	0.35571269270589845
>10	3.8022973029196785	16.46128145781756
>50	1.9573453705331356	23.805661923023447
>100	1.6237399347741284	38.357261080232846
>500	0.005762244818925409	0.6458958601345001
>1k	0.002122932301709361	0.5794784094821726
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2294	0.10589880339891766	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.045886403913916365	0.0
2	0.0	0.0	0.0	0.16508026196797276	0.0
3	0.0	0.0	0.0	0.2418961333087744	0.0
4	0.0	0.0	0.0	0.37641623492361576	0.0
5	0.0	0.0	0.0	0.660505701408768	0.0
6	0.0	0.0	0.0	0.9173587712045735	0.0
7	0.0	0.0	0.0	1.0662818486958152	0.0
8	0.0	0.0	0.0	1.4055827227071687	0.0
9	0.0	0.0	0.0	1.5145744728487747	0.0
10	0.0	0.0	0.0	1.6917495414821861	0.0
11	4.616338421923176E-5	0.0	0.0	1.9601896207170189	0.0
12	4.616338421923176E-5	0.0	0.0	2.1664476214085466	0.0
13	9.232676843846352E-5	0.0	0.0	2.2607594153684367	0.0
14	9.232676843846352E-5	0.0	0.0	2.2995828214968106	0.0
15	9.232676843846352E-5	0.0	0.0	2.3652733172407774	0.0
16	9.232676843846352E-5	0.0	0.0	2.505794658804119	0.0
17	9.232676843846352E-5	0.0	0.0	2.665704621739538	0.0
18	9.232676843846352E-5	0.0	0.0	2.850219668463807	0.0
19	9.232676843846352E-5	0.0	0.0	2.9638277570273366	0.0
20	1.3849015265769528E-4	0.0	0.0	3.073050324090039	0.0
21	1.3849015265769528E-4	0.0	0.0	3.228066968298219	0.0
22	1.3849015265769528E-4	0.0	0.0	3.3955477262455918	0.0
23	1.3849015265769528E-4	0.0	0.0	3.5681526198412996	0.0
24	1.3849015265769528E-4	0.0	0.0	3.700733859318933	0.0
25	1.3849015265769528E-4	0.0	0.0	3.81147981806087	0.0
26	1.3849015265769528E-4	0.0	0.0	3.9208870386604495	0.0
27	1.3849015265769528E-4	0.0	0.0	4.0230466079376095	0.0
28	1.3849015265769528E-4	0.0	0.0	4.1270065491993195	0.0
29	1.3849015265769528E-4	0.0	0.0	4.243245950663344	0.0
30	1.3849015265769528E-4	0.0	0.0	4.394523360749767	0.0
31	1.3849015265769528E-4	0.0	0.0	4.537537525060947	0.0
32	1.3849015265769528E-4	0.0	0.0	4.674273469118312	0.0
33	1.3849015265769528E-4	0.0	0.0	4.802099880021364	0.0
34	1.3849015265769528E-4	0.0	0.0	4.941467136979225	0.0
35	1.3849015265769528E-4	0.0	0.0	5.114764481338221	0.0
36	1.3849015265769528E-4	0.0	0.0	5.283860957733267	0.0
37	1.3849015265769528E-4	0.0	0.0	5.448987383085459	0.0
38	1.3849015265769528E-4	0.0	0.0	5.596063925207932	0.0
39	1.3849015265769528E-4	0.0	0.0	5.745263983004488	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCTA	35	1.2125929E-7	45.000004	35
ACGTTAG	30	2.1663946E-6	45.000004	1
ATAGCCG	20	7.034669E-4	45.000004	2
GAACGTA	35	1.2125929E-7	45.000004	9
CACGTTA	30	2.1663946E-6	45.000004	38
CACGTGC	30	2.1663946E-6	45.000004	37
CAGTACG	70	0.0	45.000004	1
TACGTTC	20	7.034669E-4	45.000004	31
CGAATCT	25	3.8919534E-5	45.0	38
TAGTCCG	25	3.8919534E-5	45.0	24
TAACTCG	25	3.8919534E-5	45.0	38
AAGTACG	60	3.6379788E-12	41.250004	1
TACGGGT	60	3.6379788E-12	41.250004	4
CGAATAT	250	0.0	40.499996	14
GGACTAA	560	0.0	40.178574	8
CTATGCG	45	1.929584E-8	40.0	1
ATACGTA	45	1.929584E-8	40.0	36
TGTCCGA	45	1.929584E-8	40.0	41
CTACCTT	350	0.0	39.857143	17
GACTAAT	530	0.0	39.481133	9
>>END_MODULE