##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548983_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4906009
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223534852871243	31.0	31.0	33.0	30.0	34.0
2	31.547587050900233	31.0	31.0	34.0	30.0	34.0
3	31.665996943747963	31.0	31.0	34.0	30.0	34.0
4	30.30965862475996	35.0	33.0	37.0	0.0	37.0
5	32.861808039895564	35.0	33.0	37.0	19.0	37.0
6	34.472066806237	35.0	33.0	37.0	30.0	37.0
7	35.46684973468251	36.0	35.0	37.0	33.0	37.0
8	35.63051943035571	37.0	35.0	37.0	35.0	37.0
9	37.63155611822155	39.0	37.0	39.0	35.0	39.0
10	37.067956662941306	39.0	37.0	39.0	33.0	39.0
11	36.63061217376487	39.0	35.0	39.0	32.0	39.0
12	35.63672651232397	37.0	35.0	39.0	31.0	39.0
13	35.23702728633396	37.0	35.0	39.0	30.0	39.0
14	36.07287124829979	38.0	35.0	40.0	30.0	41.0
15	36.305261364176054	38.0	35.0	40.0	31.0	41.0
16	36.42844825600605	38.0	35.0	40.0	31.0	41.0
17	36.29206734027598	38.0	35.0	40.0	31.0	41.0
18	36.30901390519259	38.0	35.0	40.0	31.0	41.0
19	36.341948618520675	37.0	35.0	40.0	31.0	41.0
20	36.27149889044231	37.0	35.0	40.0	31.0	41.0
21	36.133378882916844	37.0	34.0	40.0	30.0	41.0
22	36.014674453308174	37.0	34.0	40.0	30.0	41.0
23	35.95110485936736	37.0	34.0	40.0	30.0	41.0
24	35.9040270818908	37.0	34.0	40.0	30.0	41.0
25	35.78547817584517	36.0	34.0	40.0	30.0	41.0
26	35.609890646348184	36.0	34.0	40.0	30.0	41.0
27	35.64122935771214	36.0	34.0	40.0	30.0	41.0
28	35.65414453988976	36.0	34.0	40.0	30.0	41.0
29	35.643462537471905	36.0	34.0	40.0	30.0	41.0
30	35.5273532926662	36.0	34.0	40.0	29.0	41.0
31	35.457984483925735	36.0	34.0	40.0	29.0	41.0
32	35.37435398100574	36.0	34.0	40.0	29.0	41.0
33	35.21227722982163	36.0	34.0	40.0	29.0	41.0
34	35.00672644505952	36.0	34.0	40.0	27.0	41.0
35	34.95503758757882	36.0	33.0	40.0	27.0	41.0
36	34.87247455110661	36.0	33.0	40.0	27.0	41.0
37	34.862547541188775	35.0	33.0	40.0	27.0	41.0
38	34.81247201136402	35.0	33.0	40.0	27.0	41.0
39	34.740025955924665	35.0	33.0	40.0	27.0	41.0
40	34.632027784702395	35.0	33.0	40.0	26.0	41.0
41	34.596900250284904	35.0	33.0	40.0	27.0	41.0
42	34.52707710075542	35.0	33.0	40.0	27.0	41.0
43	34.43777192418522	35.0	33.0	40.0	27.0	41.0
44	34.32523666385447	35.0	33.0	39.0	26.0	41.0
45	34.19600514389599	35.0	33.0	39.0	26.0	41.0
46	34.114010593947135	35.0	33.0	39.0	25.0	41.0
47	34.065943009888485	35.0	33.0	39.0	25.0	41.0
48	33.96847865546109	35.0	33.0	39.0	25.0	41.0
49	33.92628488859275	35.0	33.0	39.0	25.0	41.0
50	33.82194304983949	35.0	33.0	39.0	25.0	40.0
51	33.73512706560465	35.0	33.0	38.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	2.0
12	5.0
13	13.0
14	31.0
15	112.0
16	375.0
17	952.0
18	2297.0
19	4474.0
20	7650.0
21	12499.0
22	18736.0
23	28219.0
24	39560.0
25	53824.0
26	69806.0
27	87014.0
28	106259.0
29	130130.0
30	159244.0
31	200316.0
32	253421.0
33	364879.0
34	537125.0
35	684750.0
36	383466.0
37	477449.0
38	595434.0
39	687613.0
40	352.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.46999750713869	25.13593024391109	30.07805326080731	12.31601898814291
2	28.069964812539073	26.788617795034618	31.379375781821846	13.762041610604465
3	29.99118020370529	26.32865125196468	29.799048473005247	13.881120071324776
4	23.206928483009307	38.30716576345457	25.859634582814667	12.626271170721456
5	24.157232487751244	32.67172970942369	28.413889986748902	14.757147816076163
6	22.7255188484163	40.94464563762521	25.54948431607035	10.780351197888141
7	89.40964030029296	4.259021946351912	4.402376758787031	1.9289609945680897
8	90.95201415244041	2.935909819977909	3.8856838623818257	2.22639216519986
9	86.84719086328623	4.620395111382796	5.513137052948741	3.0192769723822357
10	57.03778774152269	21.668794329566047	10.27719272426936	11.0162252046419
11	48.3168090396899	19.20049474022571	18.04536029183803	14.43733592824636
12	44.611577353404776	19.38663381987273	20.16881746446042	15.832971362262075
13	23.830918369697244	33.8986129051129	21.827497666636976	20.44297105855289
14	17.46645797021571	35.07341710950796	27.897462071512706	19.56266284876363
15	19.363274710666044	21.760335131876033	41.71219416841673	17.164195989041193
16	22.5115159796894	17.84854043276317	39.41101616405514	20.228927423492294
17	20.34174825199057	20.171446892983685	27.036334421726497	32.450470433299245
18	25.107414193492104	24.646163510910803	28.506021900897448	21.740400394699645
19	31.38946544941112	26.855617264460786	20.283350478973848	21.471566807154247
20	35.04859041228828	24.623436279876373	21.334938439778647	18.993034868056704
21	26.677223788215638	29.496521510661722	23.546838173350274	20.27941652777237
22	25.6059864545703	25.202787031169326	24.241129602493594	24.950096911766774
23	26.226083156390462	27.913422091153926	23.06815988311477	22.792334869340845
24	27.104964544500426	21.658215465972443	31.98198372648725	19.254836263039877
25	22.46053767940499	23.28352842402042	28.904308981088295	25.351624915486294
26	19.583107165111194	31.767185914253314	25.588191949912854	23.061514970722637
27	20.21188709600818	29.957935258577795	26.420171671107816	23.410005974306202
28	18.875383229015682	28.85147581262081	31.916023798570286	20.357117159793226
29	21.193825775696702	24.847589965693093	31.404671291878998	22.553912966731207
30	23.170687212355297	27.604984010424765	27.805228241529928	21.41910053569001
31	32.114352011991826	25.034748203682465	21.617714113447406	21.233185670878303
32	34.62863602573905	24.439152068412433	21.305015950847217	19.627195955001305
33	31.060318071165383	25.250259426756045	22.65238404576918	21.037038456309396
34	21.14494694159754	25.735399180882055	28.23804440635963	24.881609471160772
35	21.491725759165952	26.83735394696585	28.258284891038727	23.41263540282947
36	28.733844556746636	28.469271051072266	22.577210926437356	20.219673465743742
37	24.968115631259543	31.73353330578888	23.80490944880044	19.493441614151134
38	23.52686266984019	32.434000834486845	20.6274591016853	23.41167739398766
39	25.026350338941487	30.010360763708345	22.50266968527779	22.46061921207238
40	26.02528857978043	24.369400871461917	22.78016611873317	26.82514443002449
41	18.65589728840693	25.54614147670744	24.782221149614685	31.015740085270938
42	21.630677807562115	25.587539688573745	21.76194540205695	31.01983710180719
43	22.507561645321076	24.736073659873025	23.92415097485553	28.832213719950374
44	22.495474427380792	26.595018476321584	25.7697040506856	25.139803045612023
45	21.42484858874087	31.763292729385533	21.162007652248498	25.649851029625097
46	21.63771000012434	31.91783790041967	23.416202457027698	23.028249642428296
47	21.68173764051391	24.552665924583504	26.68195268292414	27.083643751978443
48	23.22643517368191	21.104792102908903	31.259645059762427	24.409127663646764
49	23.23558721559622	22.413737928324224	29.58115241941056	24.769522436668993
50	19.178154789361372	28.5265885162461	26.11538217724427	26.179874517148257
51	17.98959194734457	29.85775199352468	25.695223959026574	26.457432100104178
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1143.0
1	2239.0
2	3335.0
3	9027.5
4	14720.0
5	10605.5
6	6491.0
7	6744.0
8	6997.0
9	7436.5
10	7876.0
11	8167.5
12	8459.0
13	8393.5
14	8328.0
15	8289.0
16	8250.0
17	8273.5
18	8297.0
19	8233.5
20	8170.0
21	9249.0
22	10328.0
23	11076.0
24	11824.0
25	13263.0
26	21782.0
27	28862.0
28	38026.5
29	47191.0
30	54034.5
31	60878.0
32	71309.5
33	81741.0
34	90009.0
35	98277.0
36	103510.5
37	108744.0
38	135619.0
39	162494.0
40	225690.0
41	288886.0
42	335161.5
43	381437.0
44	388655.0
45	395873.0
46	382110.0
47	368347.0
48	359963.5
49	351580.0
50	335823.5
51	320067.0
52	300830.0
53	281593.0
54	279023.5
55	276454.0
56	283339.5
57	290225.0
58	299031.0
59	307837.0
60	295372.0
61	282907.0
62	248769.0
63	214631.0
64	182179.5
65	149728.0
66	126048.0
67	102368.0
68	85450.5
69	68533.0
70	59284.5
71	50036.0
72	40232.5
73	30429.0
74	23797.5
75	13275.5
76	9385.0
77	7595.5
78	5806.0
79	4653.5
80	3501.0
81	2345.5
82	1190.0
83	874.5
84	559.0
85	381.0
86	203.0
87	141.5
88	80.0
89	71.5
90	63.0
91	36.5
92	10.0
93	8.5
94	7.0
95	4.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	4906009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.744215213198345
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.21016542812545	12.156134611470538
2	9.483903466152247	2.9863323446459744
3	3.465720884333005	1.6369516641544477
4	1.7167853249965934	1.0811775052642811
5	1.0340265548034218	0.813996830749355
6	0.6625821097291088	0.6259101199194054
7	0.4914146282475864	0.5415856366230701
8	0.3371749872527122	0.4246844451051288
9	0.25893755505979205	0.3669091734276686
>10	2.4062618140121494	8.764493524912368
>50	1.0826440159515642	12.562469982294747
>100	1.8266505594967637	54.77632430652907
>500	0.020059758340658887	2.1050191234289963
>1k	0.0035316475951864238	1.0196926594310234
>5k	1.4126590380745692E-4	0.1383180720440058
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6219	0.12676291462164052	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.038316684702372E-5	0.0	0.0	0.07808791219094788	0.0
2	4.076633369404744E-5	0.0	0.0	0.25028490571460427	0.0
3	4.076633369404744E-5	0.0	0.0	0.35533974764416454	0.0
4	4.076633369404744E-5	0.0	0.0	0.5548298015759857	0.0
5	4.076633369404744E-5	0.0	0.0	0.9350370127735191	0.0
6	4.076633369404744E-5	0.0	0.0	1.3029939406959914	0.0
7	6.114950054107116E-5	0.0	0.0	1.5161407164153184	0.0
8	6.114950054107116E-5	0.0	0.0	2.0058666830819103	0.0
9	6.114950054107116E-5	0.0	0.0	2.166914084340245	0.0
10	6.114950054107116E-5	0.0	0.0	2.4242923321176133	0.0
11	6.114950054107116E-5	0.0	0.0	2.811307521042053	0.0
12	6.114950054107116E-5	0.0	0.0	3.0956119322243394	0.0
13	1.0191583423511861E-4	0.0	0.0	3.219888100490643	0.0
14	1.2229900108214233E-4	0.0	0.0	3.2723951382885765	0.0
15	1.2229900108214233E-4	0.0	0.0	3.3499123218078077	0.0
16	1.2229900108214233E-4	0.0	0.0	3.5210290074885715	0.0
17	1.2229900108214233E-4	0.0	0.0	3.716503577551529	0.0
18	1.2229900108214233E-4	0.0	0.0	3.93442001431306	4.076633369404744E-5
19	1.4268216792916606E-4	0.0	0.0	4.095345116570312	4.076633369404744E-5
20	1.6306533477618976E-4	0.0	0.0	4.24842269959146	4.076633369404744E-5
21	1.6306533477618976E-4	0.0	0.0	4.457227860772371	4.076633369404744E-5
22	1.6306533477618976E-4	0.0	0.0	4.67049693549278	4.076633369404744E-5
23	1.6306533477618976E-4	0.0	0.0	4.894263341139407	4.076633369404744E-5
24	1.6306533477618976E-4	0.0	0.0	5.075795825079	4.076633369404744E-5
25	1.6306533477618976E-4	0.0	0.0	5.226305129077423	4.076633369404744E-5
26	1.6306533477618976E-4	0.0	0.0	5.365461009142054	4.076633369404744E-5
27	1.834485016232135E-4	0.0	0.0	5.493446913774516	4.076633369404744E-5
28	2.0383166847023722E-4	0.0	0.0	5.647095225467381	4.076633369404744E-5
29	2.0383166847023722E-4	0.0	0.0	5.79872560364239	4.076633369404744E-5
30	2.0383166847023722E-4	0.0	0.0	5.96768167363737	4.076633369404744E-5
31	2.0383166847023722E-4	0.0	0.0	6.142467329350598	4.076633369404744E-5
32	2.0383166847023722E-4	0.0	0.0	6.302067525762794	4.076633369404744E-5
33	2.2421483531726092E-4	0.0	0.0	6.482438169192107	4.076633369404744E-5
34	2.2421483531726092E-4	0.0	0.0	6.664174484800171	4.076633369404744E-5
35	2.4459800216428465E-4	0.0	0.0	6.876037121008135	4.076633369404744E-5
36	2.4459800216428465E-4	0.0	0.0	7.06851536554458	4.076633369404744E-5
37	2.649811690113084E-4	0.0	0.0	7.262991160432033	4.076633369404744E-5
38	2.853643358583321E-4	0.0	0.0	7.502024558047081	4.076633369404744E-5
39	2.853643358583321E-4	0.0	0.0	7.730621774236452	4.076633369404744E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATCG	20	7.035543E-4	45.000004	1
CGTCGAT	20	7.035543E-4	45.000004	41
TAGTCCG	20	7.035543E-4	45.000004	13
ACGTTAG	150	0.0	45.0	1
TAAGACG	165	0.0	40.909092	1
GTCGATA	50	1.0822987E-9	40.5	30
CGTTTTT	4635	0.0	39.417477	1
TACGGGA	925	0.0	38.675674	4
AGGGCGA	3575	0.0	38.39161	6
AACGTTC	170	0.0	38.38235	39
CGAATAT	475	0.0	38.36842	14
CGTTAGG	695	0.0	38.20144	2
CGAACGT	165	0.0	38.18182	37
TTACGAG	255	0.0	37.941177	1
CGACCCG	1870	0.0	37.90107	32
ATAGGGC	2460	0.0	37.591465	4
ATTGGGA	2850	0.0	37.57895	4
TCGACGA	30	1.14046576E-4	37.500004	28
AATGGGC	1615	0.0	37.337463	4
TAGGGAT	4550	0.0	37.335167	5
>>END_MODULE