##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548979_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1568550
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.416926460743998	31.0	31.0	33.0	30.0	34.0
2	31.756883746134964	31.0	31.0	34.0	30.0	34.0
3	31.851863185744797	31.0	31.0	34.0	30.0	34.0
4	30.348569698128845	35.0	35.0	37.0	0.0	37.0
5	32.968681266137516	35.0	33.0	37.0	19.0	37.0
6	34.65865034586083	36.0	33.0	37.0	32.0	37.0
7	35.570107424054065	37.0	35.0	37.0	33.0	37.0
8	35.75100379331229	37.0	35.0	37.0	35.0	37.0
9	37.7857014440088	39.0	37.0	39.0	35.0	39.0
10	37.28310095310956	39.0	37.0	39.0	34.0	39.0
11	36.88267508208218	39.0	35.0	39.0	33.0	39.0
12	35.85293870134838	37.0	35.0	39.0	32.0	39.0
13	35.46850722004399	37.0	35.0	39.0	31.0	39.0
14	36.33600204010073	38.0	35.0	40.0	31.0	41.0
15	36.62144400879794	38.0	35.0	40.0	32.0	41.0
16	36.790029645213735	38.0	35.0	40.0	32.0	41.0
17	36.74918619106818	38.0	35.0	40.0	32.0	41.0
18	36.73699658920659	38.0	35.0	40.0	32.0	41.0
19	36.721410857161075	38.0	35.0	40.0	32.0	41.0
20	36.606250358611454	38.0	35.0	40.0	32.0	41.0
21	36.46224347327149	38.0	35.0	40.0	31.0	41.0
22	36.32942781549839	37.0	35.0	40.0	31.0	41.0
23	36.29414363584202	37.0	35.0	40.0	31.0	41.0
24	36.20743234197189	37.0	35.0	40.0	31.0	41.0
25	36.11441649931465	37.0	35.0	40.0	31.0	41.0
26	35.98180357655159	37.0	35.0	40.0	31.0	41.0
27	35.99181282075803	37.0	34.0	40.0	31.0	41.0
28	36.018125019922856	37.0	35.0	40.0	31.0	41.0
29	36.06455452487967	37.0	35.0	40.0	31.0	41.0
30	36.03802811513818	36.0	35.0	40.0	31.0	41.0
31	35.92224411080297	36.0	35.0	40.0	30.0	41.0
32	35.801352841796565	36.0	34.0	40.0	30.0	41.0
33	35.66528386089063	36.0	34.0	40.0	30.0	41.0
34	35.5923553600459	36.0	34.0	40.0	30.0	41.0
35	35.477206974594374	36.0	34.0	40.0	30.0	41.0
36	35.286960568678076	36.0	34.0	40.0	29.0	41.0
37	35.2383481559402	36.0	34.0	40.0	29.0	41.0
38	35.21631060533614	36.0	34.0	40.0	29.0	41.0
39	35.161998661183894	36.0	34.0	40.0	29.0	41.0
40	35.05007618501163	36.0	34.0	40.0	28.0	41.0
41	35.03432086959294	36.0	34.0	40.0	29.0	41.0
42	34.96327308660865	35.0	34.0	40.0	28.0	41.0
43	34.9226540435434	35.0	34.0	40.0	28.0	41.0
44	34.82904912180039	35.0	34.0	40.0	28.0	41.0
45	34.72791495330082	35.0	34.0	40.0	27.0	41.0
46	34.6618265276848	35.0	34.0	40.0	27.0	41.0
47	34.61728985368653	35.0	33.0	40.0	27.0	41.0
48	34.53158012176851	35.0	33.0	39.0	27.0	41.0
49	34.481378980587166	35.0	33.0	39.0	27.0	41.0
50	34.32827005833413	35.0	33.0	39.0	27.0	41.0
51	34.2432890248956	35.0	33.0	39.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	4.0
12	1.0
13	4.0
14	6.0
15	15.0
16	66.0
17	177.0
18	438.0
19	929.0
20	1699.0
21	2878.0
22	4534.0
23	6656.0
24	9824.0
25	13895.0
26	18623.0
27	23198.0
28	28158.0
29	34871.0
30	43853.0
31	55817.0
32	74054.0
33	111654.0
34	172548.0
35	241097.0
36	117244.0
37	149316.0
38	199927.0
39	256866.0
40	198.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.171464091039496	25.62723534474515	30.646903190845048	12.55439737337031
2	27.866118389595485	27.040578878582128	31.579356730738578	13.513946001083804
3	29.141818877307067	26.858244875840743	30.573969589748494	13.425966657103693
4	23.174715501577893	38.95164323738485	25.318606356188837	12.555034904848428
5	23.590131012718754	32.96152497529565	29.041981447833987	14.406362564151607
6	22.263109241018775	40.57773102546938	25.936246852188326	11.222912881323516
7	90.11290682477447	4.440661757674285	3.9159733511842147	1.5304580663670269
8	92.09091198877944	2.755984826750821	3.5551305345701447	1.597972649899589
9	88.01345191418827	4.967581524337764	4.650218354531255	2.3687482069427177
10	56.265595613783425	24.506965031398426	9.804405342513785	9.423034012304358
11	49.70667176691849	19.373370310159064	17.884288036721813	13.035669886200631
12	46.68451754805393	20.57008064773198	19.204424468458132	13.540977335755953
13	22.00337891683402	42.120238436772816	18.88400114755666	16.992381498836508
14	14.868509132638424	42.77504701794651	23.8399795989927	18.516464250422366
15	14.77236938573842	24.06247808485544	45.54212489241657	15.623027636989576
16	16.230340123043575	19.212648624525837	44.508877625832774	20.048133626597814
17	17.079659558190684	21.341111217366358	25.25045424117816	36.3287749832648
18	21.61282713333971	26.067514583532564	30.097669822447482	22.221988460680244
19	31.50043033374773	25.604220458385136	20.74457301329253	22.150776194574608
20	33.82589015332632	23.813012017468363	22.57339581141819	19.78770201778713
21	24.1185171017819	30.11169551496605	24.239520576328456	21.530266806923592
22	24.392655637372094	26.03959070479105	21.878167734531893	27.689585923304964
23	23.57973924962545	31.039048803034653	21.90175639922221	23.479455548117688
24	21.316502502311053	23.688438366644355	36.96382008861688	18.031239042427718
25	18.848618150521183	26.977973287431066	31.99037327468044	22.183035287367314
26	17.589939753275317	37.55736189474355	23.664212170475917	21.188486181505212
27	19.75429536833381	35.00570590672915	26.368556947499282	18.871441777437763
28	16.753944726020848	29.98061904306525	36.06381690095948	17.201619329954415
29	16.711995154760768	25.04051512543432	35.02215421886456	23.225335500940357
30	20.01523700232699	31.820917407797012	27.25096426636065	20.912881323515347
31	30.63364253610022	27.307194542730546	21.795734914411398	20.263428006757835
32	31.974052468840647	28.042395843294766	22.67935354308119	17.3041981447834
33	28.86124127378789	28.752287144177746	22.03614803480922	20.350323547225145
34	20.48930540945459	27.78859456185649	26.060055465238598	25.66204456345032
35	22.52991616461063	24.951196965350164	28.944694144273374	23.574192725765837
36	30.768480443721906	26.34509578910459	22.646329412514742	20.240094354658762
37	21.720059927958943	32.91995792292244	26.48860412482866	18.87137802428995
38	21.806572949539383	32.80705109814797	23.07041535175799	22.315960600554654
39	22.191705715469702	31.949124988046286	25.170571546970134	20.68859774951388
40	25.873194989002585	25.858085492971217	24.359057728475346	23.90966178955086
41	18.183481559401997	24.64052787606388	26.868445379490613	30.307545185043512
42	22.272544706894905	26.546619489337285	22.817379108093462	28.36345669567435
43	23.1316183736572	27.717318542603042	23.64177106244621	25.50929202129355
44	21.245035223614167	31.363233559657004	25.665040961397466	21.726690255331356
45	18.487839087054923	36.691657900608845	20.612093972139874	24.20840904019636
46	21.064805074750566	34.93972139874406	23.123712983328552	20.87176054317682
47	22.784546236970453	26.324503522361415	26.341270600235887	24.549679640432245
48	23.64489496668898	23.05849351311721	30.035446750183294	23.26116477001052
49	21.1643874916324	25.210226004908993	30.363775461413407	23.261611042045203
50	19.113894998565552	33.09955054030792	25.542698670746866	22.243855790379648
51	18.78072104810175	33.5603582926907	22.994294093270856	24.664626565936693
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	341.0
1	664.0
2	987.0
3	2990.5
4	4994.0
5	3649.0
6	2304.0
7	2525.0
8	2746.0
9	2888.5
10	3031.0
11	3079.0
12	3127.0
13	3047.0
14	2967.0
15	2817.0
16	2667.0
17	2640.5
18	2614.0
19	2578.5
20	2543.0
21	2887.5
22	3232.0
23	3859.5
24	4487.0
25	5715.5
26	9094.5
27	11245.0
28	16536.5
29	21828.0
30	26183.0
31	30538.0
32	32656.0
33	34774.0
34	38809.5
35	42845.0
36	41755.0
37	40665.0
38	51293.5
39	61922.0
40	86772.5
41	111623.0
42	137361.5
43	163100.0
44	159802.5
45	156505.0
46	141759.5
47	127014.0
48	121498.5
49	115983.0
50	112423.5
51	108864.0
52	98263.5
53	87663.0
54	79494.5
55	71326.0
56	69993.5
57	68661.0
58	66067.5
59	63474.0
60	62266.5
61	61059.0
62	53862.0
63	46665.0
64	39730.0
65	32795.0
66	27804.5
67	22814.0
68	18806.0
69	14798.0
70	13341.0
71	11884.0
72	10805.0
73	9726.0
74	7042.0
75	3236.5
76	2115.0
77	1478.5
78	842.0
79	597.0
80	352.0
81	220.0
82	88.0
83	55.0
84	22.0
85	16.5
86	11.0
87	7.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	1.5
96	3.0
97	1.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1568550.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.22633440085123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.89295118300153	12.801434077654145
2	7.931464150054534	2.573971791742963
3	2.4777520207192367	1.206144985517256
4	1.1015715695292543	0.7149787461460888
5	0.657002556172052	0.5330371589330879
6	0.4649794782637962	0.45269475023050154
7	0.33589293031277706	0.3815217707095857
8	0.274702814319889	0.35659357808075703
9	0.25920942893043364	0.37854169863110076
>10	3.862541502903505	16.135575051779266
>50	2.066914869085238	24.65473651592265
>100	1.6677123921812853	38.45598376387612
>500	0.004726832340632347	0.5055172055136148
>1k	0.002578272185799462	0.8492689052628727
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	2754	0.175576169073348	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2554	0.16282553951101336	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2391	0.15243377641771064	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	1901	0.12119473398999075	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.37531478116732E-5	0.0	0.0	0.05744158617831755	0.0
2	6.37531478116732E-5	0.0	0.0	0.19380956934748653	0.0
3	6.37531478116732E-5	0.0	0.0	0.27044085301711773	0.0
4	6.37531478116732E-5	0.0	0.0	0.3869816072168563	0.0
5	6.37531478116732E-5	0.0	0.0	0.6394440725510822	0.0
6	1.275062956233464E-4	0.0	0.0	0.8643014248828536	0.0
7	1.275062956233464E-4	0.0	0.0	1.0004143954607758	0.0
8	1.275062956233464E-4	0.0	0.0	1.305473207739632	0.0
9	1.275062956233464E-4	0.0	0.0	1.4029517707436805	0.0
10	1.275062956233464E-4	0.0	0.0	1.5731089222530363	0.0
11	1.275062956233464E-4	0.0	0.0	1.8572567020496638	0.0
12	1.275062956233464E-4	0.0	0.0	2.0655382359504	0.0
13	1.275062956233464E-4	0.0	0.0	2.166331962640655	0.0
14	1.275062956233464E-4	0.0	0.0	2.204647604475471	0.0
15	1.275062956233464E-4	0.0	0.0	2.263364253610022	0.0
16	1.275062956233464E-4	0.0	0.0	2.408657677472825	0.0
17	1.275062956233464E-4	0.0	0.0	2.5804086576774727	0.0
18	1.275062956233464E-4	0.0	0.0	2.7707118038953173	0.0
19	1.275062956233464E-4	0.0	0.0	2.8791559083229736	0.0
20	1.275062956233464E-4	0.0	0.0	2.9897676197762264	0.0
21	1.912594434350196E-4	0.0	0.0	3.158012176851232	0.0
22	1.912594434350196E-4	0.0	0.0	3.3296356507602565	0.0
23	1.912594434350196E-4	0.0	0.0	3.5080807114851296	0.0
24	1.912594434350196E-4	0.0	0.0	3.648082624079564	0.0
25	1.912594434350196E-4	0.0	0.0	3.7626470306971407	0.0
26	1.912594434350196E-4	0.0	0.0	3.868094737177648	0.0
27	1.912594434350196E-4	0.0	0.0	3.975391284944694	0.0
28	1.912594434350196E-4	0.0	0.0	4.082432820120493	0.0
29	1.912594434350196E-4	0.0	0.0	4.203436294667049	0.0
30	1.912594434350196E-4	0.0	0.0	4.356188836823818	0.0
31	1.912594434350196E-4	0.0	0.0	4.506582512511555	0.0
32	1.912594434350196E-4	0.0	0.0	4.643460520863218	0.0
33	1.912594434350196E-4	0.0	0.0	4.76816167798285	0.0
34	1.912594434350196E-4	0.0	0.0	4.91256255777629	0.0
35	1.912594434350196E-4	0.0	0.0	5.096107870326097	0.0
36	1.912594434350196E-4	0.0	0.0	5.2535781454209305	0.0
37	1.912594434350196E-4	0.0	0.0	5.410028370150776	0.0
38	1.912594434350196E-4	0.0	0.0	5.546842625354627	0.0
39	1.912594434350196E-4	0.0	0.0	5.701699021389181	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTATACG	30	2.1660107E-6	45.000004	1
GTCGTTT	30	2.1660107E-6	45.000004	9
TATGTCG	30	2.1660107E-6	45.000004	34
TCGCGAT	30	2.1660107E-6	45.000004	38
CCCGCAT	30	2.1660107E-6	45.000004	23
TAGGTCG	35	1.2123019E-7	45.0	1
CGCATCG	35	1.2123019E-7	45.0	21
CCAATCG	20	7.0340745E-4	45.0	23
ATTAACG	55	1.8189894E-12	45.0	1
ATTCGAT	50	2.1827873E-11	45.0	14
CGTCTAG	20	7.0340745E-4	45.0	15
TATGCGA	20	7.0340745E-4	45.0	11
CACCTCG	20	7.0340745E-4	45.0	43
CGTAGGT	20	7.0340745E-4	45.0	24
TCGACCG	20	7.0340745E-4	45.0	24
ATGTTCG	25	3.8914593E-5	45.0	1
TGTCGTA	25	3.8914593E-5	45.0	23
TACGTAG	80	0.0	45.0	1
CTATGCG	105	0.0	42.857143	1
CGTACAT	70	0.0	41.785713	35
>>END_MODULE