##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548976_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3295481
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.228530827518046	31.0	31.0	33.0	30.0	34.0
2	31.549843861943067	31.0	31.0	34.0	30.0	34.0
3	31.663015505172083	31.0	31.0	34.0	30.0	34.0
4	30.316215144314288	35.0	33.0	37.0	0.0	37.0
5	32.849364629928075	35.0	33.0	37.0	19.0	37.0
6	34.47547626583191	35.0	33.0	37.0	30.0	37.0
7	35.459917383835624	36.0	35.0	37.0	33.0	37.0
8	35.62751780392604	37.0	35.0	37.0	35.0	37.0
9	37.63456988524589	39.0	37.0	39.0	35.0	39.0
10	37.07202863557702	39.0	37.0	39.0	33.0	39.0
11	36.65577346675644	39.0	35.0	39.0	32.0	39.0
12	35.759581378257074	37.0	35.0	39.0	31.0	39.0
13	35.3691634089227	37.0	35.0	39.0	30.0	39.0
14	36.279281537353725	38.0	35.0	40.0	31.0	41.0
15	36.52465846412102	38.0	35.0	40.0	31.0	41.0
16	36.66240952382975	38.0	35.0	40.0	32.0	41.0
17	36.58767506169813	38.0	35.0	40.0	31.0	41.0
18	36.55210938858394	38.0	35.0	40.0	31.0	41.0
19	36.55634458217177	38.0	35.0	40.0	31.0	41.0
20	36.46423116989599	38.0	35.0	40.0	31.0	41.0
21	36.31597602899243	38.0	35.0	40.0	31.0	41.0
22	36.1933872475672	38.0	35.0	40.0	30.0	41.0
23	36.13876365847656	38.0	34.0	40.0	30.0	41.0
24	36.05891886495477	37.0	34.0	40.0	30.0	41.0
25	35.93806518684222	37.0	34.0	40.0	30.0	41.0
26	35.76855609241868	37.0	34.0	40.0	30.0	41.0
27	35.79478443359255	37.0	34.0	40.0	30.0	41.0
28	35.77356446600663	37.0	34.0	40.0	30.0	41.0
29	35.789208919729774	37.0	34.0	40.0	30.0	41.0
30	35.73974937194297	37.0	34.0	40.0	30.0	41.0
31	35.66560784298256	37.0	34.0	40.0	30.0	41.0
32	35.58855717875478	37.0	34.0	40.0	29.0	41.0
33	35.43547118007963	37.0	34.0	40.0	29.0	41.0
34	35.31889487452666	37.0	34.0	40.0	28.0	41.0
35	35.194851980636514	36.0	34.0	40.0	28.0	41.0
36	35.07760202531892	36.0	34.0	40.0	27.0	41.0
37	35.04167980334282	36.0	34.0	40.0	27.0	41.0
38	35.02624624447842	36.0	34.0	40.0	27.0	41.0
39	34.95246460228416	36.0	34.0	40.0	27.0	41.0
40	34.78340642837874	36.0	33.0	40.0	26.0	41.0
41	34.71991584839967	36.0	33.0	40.0	26.0	41.0
42	34.68223637156458	35.0	33.0	40.0	27.0	41.0
43	34.63389138034782	35.0	33.0	40.0	27.0	41.0
44	34.54717020064749	35.0	33.0	40.0	27.0	41.0
45	34.454979713128374	35.0	33.0	40.0	26.0	41.0
46	34.366778324620896	35.0	33.0	39.0	26.0	41.0
47	34.31722319139452	35.0	33.0	39.0	26.0	41.0
48	34.2111367050819	35.0	33.0	39.0	26.0	41.0
49	34.15080833420068	35.0	33.0	39.0	26.0	41.0
50	34.0432826042693	35.0	33.0	39.0	26.0	41.0
51	33.93402480548363	35.0	33.0	39.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	2.0
11	0.0
12	1.0
13	9.0
14	28.0
15	78.0
16	263.0
17	645.0
18	1506.0
19	2871.0
20	5053.0
21	8170.0
22	12417.0
23	18498.0
24	25667.0
25	35111.0
26	45890.0
27	56592.0
28	68267.0
29	83153.0
30	103412.0
31	128703.0
32	162070.0
33	231368.0
34	341462.0
35	437000.0
36	261822.0
37	329368.0
38	421796.0
39	513960.0
40	298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.37588078948111	25.0807090072739	30.041229186270535	12.502181016974456
2	27.848256445720672	27.110458230528412	31.143890679387926	13.897394644362993
3	30.069631716887457	26.02369729942306	30.030335480617243	13.876335503072237
4	23.701911799825275	38.72263259900451	24.979934643834998	12.595520957335212
5	23.990670861097364	32.84060809332537	28.225166523490802	14.943554522086458
6	22.918323607388423	41.7246222933769	24.64681180076596	10.710242298468721
7	89.38346177689994	4.538002191485855	4.114634555623291	1.9639014759909101
8	91.02455756837925	3.1985619094754303	3.8436270759867828	1.9332534461585424
9	87.11395999552114	5.127991938050925	4.797812519629153	2.960235546798783
10	55.78563493462715	24.1435165306673	9.852218841498402	10.218629693207152
11	48.1821925236407	21.45953807653572	16.56902285280965	13.789246547013926
12	46.16816179489428	20.386826687818864	19.111292099696524	14.333719417590329
13	24.27918716569751	35.47858415812441	20.014043473471702	20.22818520270637
14	16.511368143224008	38.116651256675425	24.467839444378527	20.904141155722034
15	17.7549802289863	24.2643790087092	41.880047252586195	16.10059350971831
16	19.7574800158156	18.894267634982572	41.66244624077638	19.685806108425446
17	19.651516728513986	20.512544299299556	26.099983583580062	33.7359553886064
18	23.524760118477396	26.637264787750254	27.171208087681283	22.666767006091067
19	32.85059146145889	26.594934093080795	20.165705704265932	20.38876874119438
20	36.88499493700616	23.42935674640515	20.96486066829091	18.720787648297776
21	27.43250530044021	27.815514639592827	22.882577687445323	21.869402372521645
22	24.771861831398816	26.53658145806333	22.177490933796918	26.514065776740935
23	26.93800995969936	29.155865259123026	22.125783762673795	21.780341018503822
24	26.064055596133006	21.774848648801193	31.624943369420123	20.536152385645675
25	22.805927268280414	24.22927032502994	28.759291890925788	24.20551051576386
26	21.13873513456761	34.500487182296	22.841764221975485	21.519013461160906
27	22.45875488282287	32.51546587584635	24.84074403706166	20.18503520426912
28	19.923768336094184	29.902524092841077	31.841360942454227	18.332346628610512
29	21.649828962752327	26.31215291485522	30.255795739681098	21.782222382711357
30	23.38593364671197	29.367306320382365	24.949468681506584	22.297291351399082
31	29.9172715606614	27.527241091664617	20.33521055044772	22.220276797226262
32	33.49353250709078	27.224523521756005	21.27883607886072	18.003107892292505
33	30.19941550262314	28.877484045576352	20.806735041106293	20.116365410694222
34	22.710796997464104	27.984594661598717	25.147102957049366	24.157505383887816
35	23.81461158477321	25.30710994844152	26.601943691983053	24.276334774802223
36	28.49920846152656	29.43294165555802	21.03419804271364	21.033651840201777
37	24.172677675884035	32.960256788007584	23.837278989015566	19.029786547092822
38	23.92245623628235	30.972959637758496	21.272220959550367	23.832363166408786
39	25.30774718470536	29.770707219977904	23.032449587783997	21.88909600753274
40	26.628889682568342	25.00102412970974	24.559328365115746	23.810757822606167
41	18.295022790299807	26.22494258046094	26.4547724596197	29.02526216961955
42	23.676695450527554	26.880233871777747	21.808440103280827	27.634630574413872
43	24.161086044798925	27.728820163126418	22.655327097925916	25.454766694148745
44	22.519535084559735	29.89924687776989	24.6414104648153	22.93980757285507
45	21.46017531279956	33.00628952192412	20.062139639099723	25.471395526176604
46	21.43338104513423	33.114346585521204	22.381679639482066	23.0705927298625
47	23.974952366589278	26.23747489364982	25.04007760930802	24.747495130452883
48	24.16994666332472	22.516591659912468	28.668682963124354	24.644778713638466
49	21.856172133900937	25.695884758552694	27.591450231392628	24.856492876153737
50	19.141454616185012	31.513730469087818	24.76372948288884	24.581085431838325
51	18.865440280189752	32.126782099487144	23.24995956584183	25.757818054481273
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	443.0
1	1205.0
2	1967.0
3	5394.0
4	8821.0
5	6670.5
6	4520.0
7	4692.5
8	4865.0
9	4967.5
10	5070.0
11	5201.5
12	5333.0
13	5226.0
14	5119.0
15	5094.0
16	5069.0
17	5265.5
18	5462.0
19	5459.0
20	5456.0
21	6326.5
22	7197.0
23	8102.0
24	9007.0
25	11530.5
26	18924.5
27	23795.0
28	30947.0
29	38099.0
30	42180.0
31	46261.0
32	52606.0
33	58951.0
34	65213.5
35	71476.0
36	73796.5
37	76117.0
38	98852.0
39	121587.0
40	161600.5
41	201614.0
42	236599.5
43	271585.0
44	272870.5
45	274156.0
46	256875.5
47	239595.0
48	231815.5
49	224036.0
50	213797.5
51	203559.0
52	193629.5
53	183700.0
54	175766.0
55	167832.0
56	173388.5
57	178945.0
58	192350.5
59	205756.0
60	201470.5
61	197185.0
62	173958.5
63	150732.0
64	122275.0
65	93818.0
66	77922.0
67	62026.0
68	52166.0
69	42306.0
70	37774.0
71	33242.0
72	26236.5
73	19231.0
74	16082.5
75	10120.0
76	7306.0
77	5508.5
78	3711.0
79	2818.0
80	1925.0
81	1476.5
82	1028.0
83	635.5
84	243.0
85	263.5
86	284.0
87	165.0
88	46.0
89	28.0
90	10.0
91	7.0
92	4.0
93	3.5
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	3295481.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	15.601908890286003
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.9732113097273	12.165309387373835
2	8.79333926936748	2.743857562440911
3	3.057251512588402	1.4309687866227996
4	1.5833806822478158	0.9881504457227726
5	0.911329016307435	0.7109236140751285
6	0.6038126764678237	0.5652378219030434
7	0.4004018429311971	0.4372923151040602
8	0.3206045042256839	0.4001633811795547
9	0.26259846981185003	0.36873336606597035
>10	2.712656233450632	10.525111563004016
>50	1.4407071667593363	16.404540654771566
>100	1.927984148393981	51.15108426546002
>500	0.00954237579079427	1.0052990514959204
>1k	0.0029687391349137735	0.9150782092189055
>5k	2.1205279535098379E-4	0.18824957556150157
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	5690	0.17266068291700057	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3873	0.1175245738027317	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3702	0.11233564994002393	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3645	0.11060600865245467	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.060021587137052225	0.0
2	0.0	0.0	0.0	0.19833220097460735	0.0
3	0.0	0.0	0.0	0.28190118528979535	0.0
4	0.0	0.0	0.0	0.41505321984863514	0.0
5	0.0	0.0	0.0	0.6960440676186572	0.0
6	0.0	0.0	0.0	0.9633495080080874	0.0
7	0.0	0.0	0.0	1.1122807262429977	0.0
8	0.0	0.0	0.0	1.4709233644496813	0.0
9	0.0	0.0	0.0	1.5758852804795416	0.0
10	0.0	0.0	0.0	1.7579527844341993	0.0
11	0.0	0.0	0.0	2.0453766840106193	0.0
12	0.0	0.0	0.0	2.25678740068597	0.0
13	0.0	0.0	0.0	2.356772804941069	0.0
14	3.0344583992442986E-5	0.0	0.0	2.3991641887785122	0.0
15	3.0344583992442986E-5	0.0	0.0	2.4676822594334484	0.0
16	3.0344583992442986E-5	0.0	0.0	2.612456269661394	0.0
17	3.0344583992442986E-5	0.0	0.0	2.769398458070309	0.0
18	3.0344583992442986E-5	0.0	0.0	2.953134914144551	0.0
19	3.0344583992442986E-5	0.0	0.0	3.06540987491659	0.0
20	3.0344583992442986E-5	0.0	0.0	3.187122001310279	0.0
21	3.0344583992442986E-5	0.0	0.0	3.341849035087746	0.0
22	3.0344583992442986E-5	0.0	0.0	3.522551032762744	0.0
23	3.0344583992442986E-5	0.0	0.0	3.7093826364042153	0.0
24	3.0344583992442986E-5	0.0	0.0	3.847389804401846	0.0
25	3.0344583992442986E-5	0.0	0.0	3.97550463801794	0.0
26	6.068916798488597E-5	0.0	0.0	4.091147847613141	0.0
27	6.068916798488597E-5	0.0	0.0	4.197050445746767	0.0
28	1.2137833596977194E-4	0.0	0.0	4.318641193804486	0.0
29	1.5172291996221494E-4	0.0	0.0	4.441809860229812	0.0
30	1.5172291996221494E-4	0.0	0.0	4.585643188353991	0.0
31	1.820675039546579E-4	0.0	0.0	4.742190897171005	0.0
32	1.820675039546579E-4	0.0	0.0	4.887541454494807	0.0
33	1.820675039546579E-4	0.0	0.0	5.029402384659478	0.0
34	1.820675039546579E-4	0.0	0.0	5.177301887038645	0.0
35	2.124120879471009E-4	0.0	0.0	5.351934967915154	0.0
36	2.124120879471009E-4	0.0	0.0	5.531848006406348	0.0
37	2.124120879471009E-4	0.0	0.0	5.713551375353097	0.0
38	2.124120879471009E-4	0.0	0.0	5.882327951519065	0.0
39	2.427566719395439E-4	0.0	0.0	6.05826584950725	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATAA	20	7.0352055E-4	45.000004	26
TACGTCG	20	7.0352055E-4	45.000004	44
TACGATA	35	1.2128476E-7	45.0	31
TATTACG	35	1.2128476E-7	45.0	1
CTCGCTA	25	3.8923972E-5	45.0	21
ACGTAAG	95	0.0	45.0	1
CGAACTA	30	2.166742E-6	44.999996	14
ACGTCAC	30	2.166742E-6	44.999996	16
AATCGGC	30	2.166742E-6	44.999996	35
TATAGCG	145	0.0	41.896553	1
CGTATTT	650	0.0	40.5	1
TCGATAG	200	0.0	40.5	1
GTACGTA	50	1.0822987E-9	40.5	23
ATAGGGT	725	0.0	40.03448	4
CGTTTCA	45	1.9301297E-8	40.0	25
TAGTACG	45	1.9301297E-8	40.0	1
CGTTAGG	395	0.0	39.873417	2
CGTCGAT	40	3.4608456E-7	39.375004	20
ATCTACC	1170	0.0	39.23077	15
ATACCGG	105	0.0	38.57143	2
>>END_MODULE