##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR3548975_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2531775
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.196793553929556	31.0	31.0	33.0	30.0	34.0
2	31.526621441478806	31.0	31.0	34.0	30.0	34.0
3	31.63126739145461	31.0	31.0	34.0	30.0	34.0
4	30.296680787194756	35.0	33.0	37.0	0.0	37.0
5	32.83897660732095	35.0	33.0	37.0	19.0	37.0
6	34.46603430399621	35.0	33.0	37.0	30.0	37.0
7	35.42967167303571	35.0	35.0	37.0	33.0	37.0
8	35.58856493961746	37.0	35.0	37.0	35.0	37.0
9	37.59749503806618	39.0	37.0	39.0	35.0	39.0
10	37.05100690227212	39.0	37.0	39.0	33.0	39.0
11	36.623930641545954	39.0	35.0	39.0	32.0	39.0
12	35.693428523466736	37.0	35.0	39.0	31.0	39.0
13	35.288030334449154	37.0	35.0	39.0	30.0	39.0
14	36.17166177879156	38.0	35.0	40.0	30.0	41.0
15	36.457570676699156	38.0	35.0	40.0	31.0	41.0
16	36.62211649929397	38.0	35.0	40.0	32.0	41.0
17	36.564643382607066	38.0	35.0	40.0	31.0	41.0
18	36.54270462422608	38.0	35.0	40.0	31.0	41.0
19	36.51578398554374	38.0	35.0	40.0	31.0	41.0
20	36.40377798185068	38.0	35.0	40.0	31.0	41.0
21	36.259849710183566	38.0	35.0	40.0	31.0	41.0
22	36.10767070533519	37.0	34.0	40.0	30.0	41.0
23	36.03982699884468	37.0	34.0	40.0	30.0	41.0
24	35.96518134510373	37.0	34.0	40.0	30.0	41.0
25	35.82677449615388	37.0	34.0	40.0	30.0	41.0
26	35.64898697554087	37.0	34.0	40.0	30.0	41.0
27	35.63263955130294	37.0	34.0	40.0	30.0	41.0
28	35.6583918397172	37.0	34.0	40.0	30.0	41.0
29	35.69321997412882	37.0	34.0	40.0	30.0	41.0
30	35.632489853956216	36.0	34.0	40.0	30.0	41.0
31	35.5446870278757	36.0	34.0	40.0	29.0	41.0
32	35.46063019028152	36.0	34.0	40.0	29.0	41.0
33	35.312422312409275	36.0	34.0	40.0	29.0	41.0
34	35.2295579188514	36.0	34.0	40.0	28.0	41.0
35	35.10884103050232	36.0	34.0	40.0	28.0	41.0
36	34.93411934314858	36.0	33.0	40.0	27.0	41.0
37	34.87961015493083	36.0	33.0	40.0	27.0	41.0
38	34.84766576808761	35.0	33.0	40.0	27.0	41.0
39	34.7483180772383	36.0	33.0	40.0	27.0	41.0
40	34.61254376869982	35.0	33.0	40.0	26.0	41.0
41	34.559851487592695	35.0	33.0	40.0	26.0	41.0
42	34.506847172438306	35.0	33.0	40.0	26.0	41.0
43	34.44568573431683	35.0	33.0	40.0	26.0	41.0
44	34.37260459559005	35.0	33.0	40.0	26.0	41.0
45	34.26395355037474	35.0	33.0	39.0	26.0	41.0
46	34.1742508714242	35.0	33.0	39.0	26.0	41.0
47	34.10833111157192	35.0	33.0	39.0	26.0	41.0
48	34.00639748792843	35.0	33.0	39.0	25.0	41.0
49	33.946639413060005	35.0	33.0	39.0	25.0	41.0
50	33.821625934374104	35.0	33.0	39.0	24.0	40.0
51	33.71304875038264	35.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	4.0
13	5.0
14	22.0
15	55.0
16	223.0
17	529.0
18	1214.0
19	2367.0
20	4234.0
21	6674.0
22	10173.0
23	14916.0
24	20747.0
25	27871.0
26	36246.0
27	44995.0
28	53858.0
29	65370.0
30	80923.0
31	100660.0
32	127158.0
33	181009.0
34	265952.0
35	348996.0
36	203244.0
37	251671.0
38	316172.0
39	366280.0
40	206.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	32.133305684746865	25.072962644784784	30.883352588598907	11.91037908186944
2	29.17861974306564	26.44583345676452	31.113467823957503	13.26207897621234
3	29.81264132871207	26.073367499086608	30.142093985444994	13.971897186756326
4	23.44323646453575	38.159947072705904	25.567714350603826	12.829102112154517
5	24.313534970524632	32.9703468910152	28.06351275291051	14.652605385549663
6	22.64664119046914	42.076250851675205	24.654126057805296	10.62298190005036
7	89.64722378568396	4.390121555035499	4.161151761116213	1.8015028981643315
8	90.9277483188672	3.1734652565887567	3.8479327744369067	2.0508536501071384
9	87.02613778870555	5.362206357199988	4.911534397803912	2.7001214562905473
10	56.856158228910544	22.9899971363964	9.843370757670014	10.310473877023037
11	48.157675938817626	20.738572740468644	16.552932231339675	14.550819089374055
12	44.3347848841228	20.776016826139767	19.67765697978691	15.21154130995053
13	23.68117230006616	37.49480107829487	20.54230727454059	18.28171934709838
14	16.9035953036901	39.27375062949906	23.746343968164627	20.076310098646207
15	16.347621727839165	23.77766586683256	41.928370412062684	17.946341993265595
16	18.823513147890313	19.25882039280742	41.66345745573757	20.254209003564693
17	19.540717480818792	20.75148858014634	25.207058289144967	34.500735649889904
18	22.966219352035626	25.5418036752871	28.269376228140334	23.222600744536937
19	32.36349991606679	25.024538120488586	20.845414778169467	21.766547185275154
20	35.39102013409565	23.595027204234185	21.69825517670409	19.315697484966083
21	26.57791470411075	27.98747914012896	23.142893819553475	22.29171233620681
22	25.55337658362216	25.1660988831946	21.759082066929327	27.521442466253916
23	25.32511775335486	29.87880044632718	21.650423122117882	23.145658678200075
24	24.53914743608733	23.464486378133916	32.19342161132012	19.802944574458632
25	21.15420999101421	25.729656071333352	29.78337332503876	23.33276061261368
26	21.309792536856552	34.3815307442407	22.63289589319746	21.675780825705285
27	22.689733487375456	32.49392224822506	25.076754450928696	19.739589813470786
28	19.047150714419725	28.90434576532275	33.02007484867337	19.028428671584166
29	19.786671406424347	25.15993719821074	31.6553011227301	23.39809027263481
30	22.16271193135251	29.727602176338735	26.016845888753938	22.092840003554816
31	30.60639274817075	26.21299286073999	20.925200699114257	22.255413691974997
32	33.176289362206354	26.797681468534922	21.100887717115462	18.92514145214326
33	29.82247632589784	26.859298318373476	21.133750037029355	22.18447531869933
34	21.923156678614806	26.96499491463499	24.710450178234638	26.401398228515564
35	23.710795785565463	24.037088603845127	27.108175094548294	25.14394051604112
36	30.77694502868541	25.571150674921743	22.235269721835472	21.416634574557374
37	23.809580235210476	32.03712810182579	25.308923581281906	18.84436808168182
38	24.127301991685677	31.19633458739422	21.252954942678556	23.42340847824155
39	24.0383920372071	30.154140869548048	23.98313436225573	21.82433273098913
40	26.953816986106588	25.323340344224903	23.953708366659754	23.76913430300876
41	19.06990155128319	24.27407648783956	26.189136080417892	30.46688588045936
42	23.527959633063762	25.99820284187971	22.425136514895676	28.048701010160855
43	23.67773597574824	26.86818536402327	23.29697544213052	26.157103218097976
44	22.046153390407916	29.683127450109115	25.3830612909915	22.887657868491473
45	20.61107325887964	33.73111749661798	20.35674576137295	25.301063483129425
46	22.376435504734822	31.90394091102092	23.040949531455205	22.678674052789052
47	23.993008857422165	25.04243070573017	25.888279961686962	25.076280475160708
48	25.192483534279308	21.485163571012432	29.267885179370207	24.054467715338053
49	21.504201597693317	24.31839322214652	29.27997156145392	24.897433618706245
50	20.167866417829387	31.67066583720907	24.96635759496796	23.19511014999358
51	19.85429194932409	32.168419389558714	22.68665264488353	25.29063601623367
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	289.0
1	939.0
2	1589.0
3	3849.0
4	6109.0
5	4658.0
6	3207.0
7	3414.5
8	3622.0
9	3845.5
10	4069.0
11	4102.0
12	4135.0
13	4059.5
14	3984.0
15	3947.5
16	3911.0
17	3994.5
18	4078.0
19	4184.5
20	4291.0
21	4234.0
22	4177.0
23	4584.0
24	4991.0
25	7080.5
26	13516.0
27	17862.0
28	24208.0
29	30554.0
30	33438.5
31	36323.0
32	38478.0
33	40633.0
34	47433.0
35	54233.0
36	53585.5
37	52938.0
38	71914.0
39	90890.0
40	124703.5
41	158517.0
42	188163.0
43	217809.0
44	218566.0
45	219323.0
46	210042.0
47	200761.0
48	189578.0
49	178395.0
50	165746.5
51	153098.0
52	149177.0
53	145256.0
54	138053.0
55	130850.0
56	132068.5
57	133287.0
58	134069.5
59	134852.0
60	134343.5
61	133835.0
62	121751.0
63	109667.0
64	92824.0
65	75981.0
66	65656.5
67	55332.0
68	45114.0
69	34896.0
70	31372.0
71	27848.0
72	23190.5
73	18533.0
74	14423.5
75	8474.0
76	6634.0
77	4677.5
78	2721.0
79	2068.0
80	1415.0
81	1118.0
82	821.0
83	603.5
84	386.0
85	252.5
86	119.0
87	84.0
88	49.0
89	28.5
90	8.0
91	6.5
92	5.0
93	5.0
94	5.0
95	3.5
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	2531775.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	16.244024661419374
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.0950270070205	12.848215692976725
2	7.970711857577055	2.58952879967099
3	2.7818247902249293	1.355640914884846
4	1.4479103172925394	0.9407956360649424
5	0.851034066957816	0.6912109185685393
6	0.5652828597926213	0.5509481229089406
7	0.3872274339056082	0.4403092390158588
8	0.30163325678414593	0.39197904495247265
9	0.2403140283926596	0.35133003033258575
>10	2.9522249170591817	12.039576897618588
>50	1.545857224933777	18.302028348266667
>100	1.8492914741026867	47.75342515074061
>500	0.009805644099821183	1.0437780104142311
>1k	0.0018551218567229267	0.7012331935839895
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	3513	0.13875640607873924	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	3071	0.12129829862448283	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	2676	0.10569659626151615	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2674	0.10561760030018466	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.038273543265100574	0.0
2	0.0	0.0	0.0	0.12378667140642435	0.0
3	0.0	0.0	0.0	0.18425807980566994	0.0
4	3.9497980665738466E-5	0.0	0.0	0.33909016401536474	0.0
5	3.9497980665738466E-5	0.0	0.0	0.5905738069141215	0.0
6	3.9497980665738466E-5	0.0	0.0	0.8467972074927669	0.0
7	7.899596133147693E-5	0.0	0.0	0.9784439770516732	0.0
8	7.899596133147693E-5	0.0	0.0	1.3011029811100907	0.0
9	7.899596133147693E-5	0.0	0.0	1.3944367094232308	0.0
10	7.899596133147693E-5	0.0	0.0	1.5551144947714548	0.0
11	7.899596133147693E-5	0.0	0.0	1.8145372317840252	0.0
12	7.899596133147693E-5	0.0	0.0	2.0179123342319123	0.0
13	1.184939419972154E-4	0.0	0.0	2.0990016885386735	0.0
14	1.184939419972154E-4	0.0	0.0	2.129573125573955	0.0
15	1.184939419972154E-4	0.0	0.0	2.1793800791934514	0.0
16	1.184939419972154E-4	0.0	0.0	2.297992515132664	0.0
17	1.5799192266295386E-4	0.0	0.0	2.437183398998726	0.0
18	1.5799192266295386E-4	0.0	0.0	2.5908700417691146	0.0
19	1.5799192266295386E-4	0.0	0.0	2.68850904997482	0.0
20	1.5799192266295386E-4	0.0	0.0	2.7905718320150883	0.0
21	1.5799192266295386E-4	0.0	0.0	2.932883056353744	0.0
22	1.5799192266295386E-4	0.0	0.0	3.0879126304667674	0.0
23	1.5799192266295386E-4	0.0	0.0	3.252540213881565	0.0
24	1.5799192266295386E-4	0.0	0.0	3.37391750846738	0.0
25	1.5799192266295386E-4	0.0	0.0	3.4787056511735837	0.0
26	1.5799192266295386E-4	0.0	0.0	3.5776480927412586	0.0
27	2.369878839944308E-4	0.0	0.0	3.6714557968223875	0.0
28	2.369878839944308E-4	0.0	0.0	3.773281590978661	0.0
29	2.369878839944308E-4	0.0	0.0	3.885653345972687	0.0
30	2.369878839944308E-4	0.0	0.0	4.021486901482162	0.0
31	2.369878839944308E-4	0.0	0.0	4.157833930740291	0.0
32	2.369878839944308E-4	0.0	0.0	4.2848199385806405	0.0
33	2.369878839944308E-4	0.0	0.0	4.408764601909727	0.0
34	2.369878839944308E-4	0.0	0.0	4.536896051189383	0.0
35	2.369878839944308E-4	0.0	0.0	4.708001303433362	0.0
36	2.369878839944308E-4	0.0	0.0	4.859594553228466	0.0
37	2.369878839944308E-4	0.0	0.0	5.025604565966565	0.0
38	2.369878839944308E-4	0.0	0.0	5.161082639650048	0.0
39	2.369878839944308E-4	0.0	0.0	5.314413800594445	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTT	20	7.0348964E-4	45.0	14
CTCCGAT	25	3.892141E-5	45.0	12
ATTACGA	35	1.212702E-7	45.0	31
CTACGTC	20	7.0348964E-4	45.0	20
CATACCG	25	3.892141E-5	45.0	1
CGTAAGA	25	3.892141E-5	45.0	37
TCGACCA	20	7.0348964E-4	45.0	44
TCGGTAA	20	7.0348964E-4	45.0	27
TACCGTT	20	7.0348964E-4	45.0	16
GTCGATA	30	2.166542E-6	44.999996	11
GTCGTAC	60	0.0	44.999996	9
TAAACGG	225	0.0	43.000004	2
GAATAAC	90	0.0	42.5	9
TACCGGT	90	0.0	42.5	40
AACGCAC	55	6.184564E-11	40.909092	15
ATAGGGC	1170	0.0	40.769234	4
CGTTGAT	800	0.0	40.5	25
AGTAATG	465	0.0	40.161293	1
CGTTAGG	290	0.0	39.56897	2
CGGATAT	40	3.4604273E-7	39.375	43
>>END_MODULE